PPP2R1A (BC001537) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	alpha isoform of regulatory subunit A, protein phosphatase 2; medium tumor antigen-associated 61 KDA protein; MGC786; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PR65A; protein phosphatase 2 (formerly 2A), regulatory subunit A
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC001537, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGACGAACTCCGCAATGAGG ACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGGTTGAAAGGAC CCGAAGTGAGCTTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCA GAACAGCTGGGAACCTTCACTACCCTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGG AGTCGCTGGCCACAGTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACA CGAGCACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGCGACTGG TTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGG CGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGGCCGCAGCCTC CAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTGGACAACGTCAAGAGTGAGATCATCCCCATGTTCTCC AACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGGCGGTGGAGGCGTGCGTGAACATCGCCCAGC TTCTGCCCCAGGAGGATCTGGAGGCCCTGGTGATGCCCACTCTGCGCCAGGCCGCTGAAGACAAGTCCTG GCGCGTCCGCTACATGGTGGCTGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAG ACAGACCTGGTCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTGAGGGCCGCAGCCTCCC ACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGCTGACTGTCGGGAGAATGTGATCATGTCCCAGATCTT GCCCTGCATCAAGGAGCTGGTGTCCGATGCCAACCAACATGTCAAGTCTGCCCTGGCCTCAGTCATCATG GGTCTCTCTCCCATCTTGGGCAAAGACAACACCATCGAGCACCTCTTGCCCCTCTTCCTGGCTCAGCTGA AGGATGAGTGCCCTGAGGTACGGCTGAACATCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCAT CCGGCAGCTGTCCCAGTCCCTGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGG CTGGCCATCATTGAGTACATGCCCCTCCTGGCTGGACAGCTGGGAGTGGAGTTCTTTGATGAGAAACTTA ACTCCTTGTGCATGGCCTGGCTTGTGGATCATGTATATGCCATCCGCGAGGCAGCCACCAGCAACCTGAA GAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCCACAATCATCCCCAAGGTCTTGGCCATGTCC GGAGACCCCAACTACCTGCACCGCATGACTACGCTCTTCTGCATCAATGTGCTGTCTGAGGTCTGTGGGC AGGACATCACCACCAAGCACATGCTACCCACGGTTCTGCGCATGGCTGGGGACCCGGTTGCCAATGTCCG CTTCAATGTGGCCAAGTCTCTGCAGAAGATAGGGCCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTC AAGCCCATCCTAGAGAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAGGAGGCTCTGA CTGTTCTGTCTCTCGCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001537 unedited AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTCTGACAGGAAAGGACGGA GCCAAGATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGAC GAACTCCGCAATGAGGACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATC GCCTTGGCCCTTGGGGTTGAAAGGACCCGAAGTGAGCTTCTGCCTTTCCTTACAGATACC ATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACC CTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACA GTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACACGAG CACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGC GACTGGTTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTG TCCAGTGCTGTGAAGGCGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACC CCCATGGTGCGGCGGGCCGCAGCCTNCAAGCTGGNGGAGTTTGCCAAGGTGCTGGAGCTG GNACACGTCAAGAGTGAGATCATCCCCCATGTCTCCAACCTGGCCTCTGACGAGCANGAC NNTCGTGCGCTGCTGGCNGNGGNNAGCTTGCCTAAACATCNNCCAGCTTCTGCCCCAGGA GATCTGGAGGCCCTGNGGATGCCCACTCTGCGCCACGCCGCTGAAAACAGTCCTGGCGCG TCCGCTACATGTTGCTGACAAGTCACAGAGCTCAAAAGCAGGGGGGCTGAAATCACCAGA AGAACTGGTCCCTGCTCCAAACCTGATGAAGACTGGAGGCGAGGTGANGCCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC001537 unedited TTTCTCTGTGAAGGGGGAAATAAAGGATAAATAAAACGGCACAGTTGACACACAAAAAAA AACCAATGATGGGGAGGACGGGAGGTGGAGAAGTAAATGGGGGAGGGGTTCCCATTACAG CAGCAGGATCCAGTGACCCGGGATGCTCACATCTCTCCCTGACGTGGGCGGAGTAGCCCC TTCCTCCCAAGGTCACTGTCCTGTCCAACCCCGTGCTCCCCTAGCCCGTTGGGAGGTGGA CAGTGAGACATCTTCCCAGGCTGGGGAGAGGAGGAACCGTGCTGGGGGAAGGGGCCTGGG GTCAGACCATGCACAGGGAGTGACAGCCAAAGGCCCCCCAGTGTCTCCCCAAAGAGGGAC ACGTGGGGGTTGGAGGGTGGACACCAGAGGCCAGCGTTTGCTCCTCTTCCAGCATCAGGC GAGAGACAGAACAGTCAGAGCCTCCTGGGCAAAGTATTTGACGTCCACATCCTGGTCCTG GGTCAGCTTCTCTAGGATGGGCTCGACTTCACTCTGCCAGGTGCTGTTGCCCAGGATGGG CCCTATCCTCTGAACAGACTTGGCCACATTGAAGCCGGACATTGGCACCCGGTCCCCCAC CTTGCCCATTACCGTGGGCACCCTGTGCTTGTTGGTGAGGTCTTGCTACAGACCTATTAC GTACAATTGCGCCTAAAAACTTTTCCCTGCCGCTCCGGTTAACTGGGCCCTCCCGCCTTG GCCCACACCTTTGGGAAAATTTGCCCTGGGCCCCCTCCTTTCCCAACTTTTCCACTACCC TTTTCAGGTTTCTCGGTGCTGTCTCCGCGTGGGTTTTACTCATTCCCAACCTCGCCACTC ACTCGATTACCCCCCCACACCAACTTCCCCCCCCCCTTCTCCCGGAGTTATTTACCCACA CGGTCACCCCCCTCTCCTGGCGTCCCCCCCACCCTTCATCCCTGAACCCCCCTGGACACT TCTCCGCCCTCCTTTCTCCCATTCTCTCATCCTCCTCCTCCCCAGG
Restriction Sites	NotI-NotI
ACCN	BC001537
Insert Size	2390 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001537.2, AAH01537.1
RefSeq Size	2282 bp
Locus ID	5518
Protein Families	Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways	Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
Gene Summary	This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

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