United States
Germany
Japan
United Kingdom
China
PCB (PC) (NM_000920) Human Untagged Clone
CAT＃: SC122580
PC (untagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 8,208.00
| Cited in 1 publication. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PCB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000920 edited
CAGCAGCGGTAGAGGCGGCGGCGAGGACTGGCGACGGCGAGGAGATAGTGTCTGCCTTCT GGAGAGCTGACCAAACACTAAGGATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGC TCCTGGGAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCCCCAAATGTCCGGCGCCTGG AGTATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGT TCCGGGCCTGCACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAGGACACGG GCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCG TGCAGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATG CAGTGCACCCTGGCTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGG ATGCAGGGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGG TGGAGGCCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCC CCATCACGTCCCTGCATGAGGCCCACGAGTTCTCCAACACCTACGGCTTCCCCATCATCT TCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTACGAGGAGC TGGAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGC TGTTTGTGGAGAAGTTCATCGAGAAGCCACGGCACATCGAGGTGCAGATCTTGGGGGACC AGTATGGGAACATCCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGGCGGCACCAGA AGGTGGTCGAGATTGCCCCCGCCGCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCA GCGACTCTGTGAAACTCGCTAAACAGGTGGGCTACGAGAACGCAGGCACCGTGGAGTTCC TGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAACTCCCGCCTGCAGGTGGAGC ACACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCACGTGGCTG AGGGCAGGAGCCTACCCGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAACGGGTGTG CCATCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGCTTCCAGCCGGACACCGGCC GCATTGAGGTGTTCCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCT TCCAAGGAGCCGTCATCTCGCCCCACTACGACTCCCTGCTGGTCAAAGTCATTGCCCACG GCAAAGACCACCCCACGGCCGCCACCAAGATGAGCAGGGCCCTTGCGGAGTTCCGCGTCC GAGGTGTGAAGACCAACATCGCCTTCCTGCAGAATGTGCTCAACAACCAGCAGTTCCTGG CAGGCACTGTGGACACCCAGTTCATCGACGAGAACCCAGAGCTGTTCCAGCTGCGGCCTG CACAGAACCGGGCCCAAAAGCTGTTGCACTACCTCGGCCATGTCATGGTAAACGGTCCAA CCACCCCGATTCCCGTCAAGGCCAGCCCCAGCCCCACGGACCCCGTTGTCCCTGCAGTGC CCATAGGCCCGCCCCCGGCTGGTTTCAGAGACATCCTGCTGCGAGAGGGGCCTGAGGGCT TTGCTCGAGCTGTGCGGAACCACCCGGGGCTGCTGCTGATGGACACGACCTTCAGGGACG CCCACCAGTCACTGCTGGCCACTCGTGTGCGCACCCACGATCTCAAAAAGATCGCCCCCT ATGTTGCCCACAACTTCAGCAAGCTCTTCAGCATGGAGAACTGGGGAGGAGCCACGTTTG ACGTCGCCATGCGCTTCCTGTATGAGTGCCCCTGGCGGCGGCTGCAGGAGCTCCGGGAGC TCATCCCCAACATCCCTTTCCAGATGCTGCTGCGGGGGGCCAATGCTGTGGGCTACACCA ACTACCCAGACAACGTGGTCTTCAAGTTCTGTGAAGTGGCCAAAGAGAATGGCATGGATG TCTTCCGTGTGTTTGACTCCCTCAACTACTTGCCCAACATGCTGCTGGGCATGGAGGCGG CAGGAAGTGCCGGAGGCGTGGTGGAGGCTGCCATCTCATACACGGGCGACGTGGCCGACC CCAGCCGCACCAAGTACTCACTGCAGTACTACATGGGCTTGGCCGAAGAGCTGGTGCGAG CTGGCACCCACATCCTGTGCATCAAGGACATGGCCGGGCTGCTGAAGCCCACGGCCTGCA CCATGCTGGTCAGCTCCCTCCGGGACCGCTTCCCCGACCTCCCACTGCACATCCACACCC ACGACACGTCAGGGGCAGGCGTGGCAGCCATGCTGGCCTGTGCCCAGGCTGGAGCTGATG TGGTGGATGTGGCAGCTGATTCCATGTCTGGGATGACTTCACAGCCCAGCATGGGGGCCC TGGTGGCCTGTACCAGAGGGACTCCCCTGGACACAGAGGTGCCCATGGAGCGCGTGTTTG ACTACAGTGAGTACTGGGAGGGGGCTCGGGGACTGTACGCGGCCTTCGACTGCACGGCCA CCATGAAGTCTGGCAACTCGGACGTGTATGAAAATGAGATCCCAGGGGGCCAGTACACCA ACCTGCACTTCCAGGCCCACAGCATGGGGCTTGGCTCCAAGTTCAAGGAGGTCAAGAAGG CCTATGTGGAGGCCAACCAGATGCTGGGCGATCTCATCAAGGTGACGCCCTCCTCCAAGA TCGTGGGGGACCTGGCCCAGTTTATGGTGCAGAATGGATTGAGCCGGGCAGAGGCCGAAG CTCAGGCGGAAGAGCTGTCCTTTCCCCGCTCCGTGGTGGAGTTCCTGCAGGGCTACATCG GTGTCCCCCATGGGGGGTTCCCCGAACCCTTTCGCTCTAAGGTACTGAAGGACCTGCCAA GGGTGGAGGGGCGGCCTGGAGCCTCCCTCCCTCCCCTGGATCTGCAGGCACTGGAGAAGG AGCTGGTAGACCGGCATGGGGAGGAGGTGACGCCGGAAGATGTGCTCTCAGCAGCTATGT ACCCCGATGTGTTTGCCCACTTCAAGGACTTCACTGCCACCTTTGGCCCCCTGGATAGCC TGAATACTCGCCTCTTCCTGCAGGGACCCAAGATCGCAGAGGAGTTTGAGGTGGAGCTGG AGCGGGGCAAGACGCTGCACATCAAAGCCCTGGCCGTGAGCGACCTGAACCGGGCCGGCC AGAGGCAGGTCTTCTTTGAGCTCAATGGGCAGCTGCGGTCCATCTTGGTCAAGGACACCC AGGCCATGAAGGAGATGCACTTCCACCCCAAGGCCCTAAAGGACGTGAAGGGCCAGATCG GGGCGCCCATGCCTGGGAAGGTGATAGACATCAAAGTGGTGGCAGGGGCCAAGGTGGCCA AGGGCCAGCCCCTGTGTGTGCTCAGTGCCATGAAGATGGAGACTGTGGTGACCTCACCCA TGGAGGGTACTGTCCGCAAGGTTCATGTGACCAAGGACATGACACTGGAAGGTGACGACC TCATCCTGGAGATCGAGTGATCTTGCCCCAGACCGGCAGCCTGGCCATCCCCAAGCCTTC AACAGAAGCTGTGCTGCCACGGCAGGCCCAGGCCAGCCAGTGCCCGAGGCCAGGAAGGCC GGGCCGTGGAGGTCCTGTCCACAGCTGGACAGGAGAGACACCGCCTGCGGTGGTTCATTC CTTTCAGCCATCGTCCTTTCCTCCGGCGGACAGCTGCTTACATGTTCATCTCTTGCCAAA TAAGGGTCCCCTCCTCACTGGAGACTACAAGTGGTGGGTCAGGTGGTCCTAGGACCCAGG GGAGGTTTAGGGGTCCTATCTCCTGGGGGAAGGGGAGATCTAAGATGTCCCAGGTCCTGG GAAGTTTACTCAATAAAGCTGGCTTTCCCCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_000920 unedited
GAAGCGTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCAGCAG CGGTAGAGGCGGCGGCGAGGACTGGCGACGGCGAGGAGATAGTGTCTGCCTTCTGGAGAG CTGACCAAACACTAAGGATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGG GAATCCGCCGAACCTCCACCGCCCCCGCTGCCTCCCCAAATGTCCGGCGCCTGGAGTATA AGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGAGATTGCCATCCGTGTGTTCCGGG CCTGCACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAGGACACGGGCCAGA TGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCGTGCAGG CCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGC ACCCTGGCTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAG GGGTCCGGTTTATTGGGCCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGGTGGAGG CCCGGGCCATCGCCATTGCTGCGGGTGTTCCCGTTGTCCCTGGCACAGATGCCCCCATCA CGTCCCTGCATGAGGCCCACGAGTTCTCCAACACCTACGGCTTCCCCATCATCTTCAAGG CGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTACGAGGAGCTGGAGG AGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGTTTG TGGAGAAGTTCATCGAGAAGCACGGCACATCGAGGTGCAGATCTTTGGGGACCAGTATGG GAACATCCT |
| Restriction Sites | Please inquire |
| ACCN | NM_000920 |
| Insert Size | 4058 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000920.2, NP_000911.1 |
| RefSeq Size | 4111 bp |
| RefSeq ORF | 3537 bp |
| Locus ID | 5091 |
| UniProt ID | P11498 |
| Protein Families | Druggable Genome |
| Protein Pathways | Citrate cycle (TCA cycle), Metabolic pathways, Pyruvate metabolism |
| Gene Summary | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) results from the use of a more distal promoter. Transcript variants 1-3 encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
H2S-induced S-sulfhydration of pyruvate carboxylase contributes to gluconeogenesis in liver cells
,Ju, Y;Untereine, A;Wu, L;Yang, G;,
Biochim. Biophys. Acta
,PubMed ID 26272431
[PC]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221895 | PC (Myc-DDK-tagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 8,200.00 |
|
| RC221895L1 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 10,600.00 |
|
| RC221895L2 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 10,600.00 |
|
| RC221895L3 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC221895L4 | Lenti ORF clone of Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 11,500.00 |
|
| RG221895 | PC (tGFP-tagged) - Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 9,800.00 |
