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GJB2 (NM_004004) Human Untagged Clone
CAT＃: SC122666
GJB2 (untagged)-Human gap junction protein, beta 2, 26kDa (GJB2)
CNY 2,400.00
CNY 2,950.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BAPS; CX26; DFNA3; DFNA3A; DFNB1; DFNB1A; HID; KID; NSRD1; PPK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004004 edited
CGGAGCCCCTCGGCGGCGCCCGGCCCAGGACCCGCCTAGGAGCGCAGGAGCCCCAGCGCA GAGACCCCAACGCCGAGACCCCCGCCCCGGCCCCGCCGCGCTTCCTCCCGACGCAGAGCA AACCGCCCAGAGTAGAAGATGGATTGGGGCACGCTGCAGACGATCCTGGGGGGTGTGAAC AAACACTCCACCAGCATTGGAAAGATCTGGCTCACCGTCCTCTTCATTTTTCGCATTATG ATCCTCGTTGTGGCTGCAAAGGAGGTGTGGGGAGATGAGCAGGCCGACTTTGTCTGCAAC ACCCTGCAGCCAGGCTGCAAGAACGTGTGCTACGATCACTACTTCCCCATCTCCCACATC CGGCTATGGGCCCTGCAGCTGATCTTCGTGTCCACGCCAGCGCTCCTAGTGGCCATGCAC GTGGCCTACCGGAGACATGAGAAGAAGAGGAAGTTCATCAAGGGGGAGATAAAGAGTGAA TTTAAGGACATCGAGGAGATCAAAACCCAGAAGGTCCGCATCGAAGGCTCCCTGTGGTGG ACCTACACAAGCAGCATCTTCTTCCGGGTCATCTTCGAAGCCGCCTTCATGTACGTCTTC TATGTCATGTACGACGGCTTCTCCATGCAGCGGCTGGTGAAGTGCAACGCCTGGCCTTGT CCCAACACTGTGGACTGCTTTGTGTCCCGGCCCACGGAGAAGACTGTCTTCACAGTGTTC ATGATTGCAGTGTCTGGAATTTGCATCCTGCTGAATGTCACTGAATTGTGTTATTTGCTA ATTAGATATTGTTCTGGGAAGTCAAAAAAGCCAGTTTAACGCATTGCCCAGTTGTTAGAT TAAGAAATAGACAGCATGAGAGGGATGAGGCAACCCGTGCTCAGCTGTCAAGGCTCAGTC GCCAGCATTTCCCAACACAAAGATTCTGACCTTAAATGCAACCATTTGAAACCCCTGTAG GCCTCAGGTGAAACTCCAGATGCCACAATGGAGCTCTGCTCCCCTAAAGCCTCAAAACAA AGGCCTAATTCTATGCCTGTCTTAATTTTCTTTCACTTAAGTTAGTTCCACTGAGACCCC AGGCTGTTAGGGGTTATTGGTGTAAGGTACTTTCATATTTTAAACAGAGGATATCGGCAT TTGTTTCTTTCTCTGAGGACAAGAGAAAAAAGCCAGGTTCCACAGAGGACACAGAGAAGG TTTGGGTGTCCTCCTGGGGTTCTTTTTGCCAACTTTCCCCACGTTAAAGGTGAACATTGG TTCTTTCATTTGCTTTGGAAGTTTTAATCTCTAACAGTGGACAAAGTTACCAGTGCCTTA AACTCTGTTACACTTTTTGGAAGTGAAAACTTTGTAGTATGATAGGTTATTTTGATGTAA AGATGTTCTGGATACCATTATATGTTCCCCCTGTTTCAGAGGCTCAGATTGTAATATGTA AATGGTATGTCATTCGCTACTATGATTTAATTTGAAATATGGTCTTTTGGTTATGAATAC TTTGCAGCACAGCTGAGAGGCTGTCTGTTGTATTCATTGTGGTCATAGCACCTAACAACA TTGTAGCCTCAATCGAGTGAGACAGACTAGAAGTTCCTAGTGATGGCTTATGATAGCAAA TGGCCTCATGTCAAATATTTAGATGTAATTTTGTGTAAGAAATACAGACTGGATGTACCA CCAACTACTACCTGTAATGACAGGCCTGTCCAACACATCTCCCTTTTCCATGACTGTGGT AGCCAGCATCGGAAAGAACGCTGATTTAAAGAGGTCGCTTGGGAATTTTATTGACACAGT ACCATTTAATGGGGAGGACAAAATGGGGCAGGGGAGGGAGAAGTTTCTGTCGTTAAAAAC AGATTTGGAAAGACTGGACTCTAAATTCTGTTGATTAAAGATGAGCTTTGTCTACTTCAA AAGTTTGTTTGCTTACCCCTTCAGCCTCCAATTTTTTAAGTGAAAATATAACTAATAACA TGTGAAAAGAATAGAAGCTAAGGTTTAGATAAATATTGAGCAGATCTATAGGAAGATTGA ACCTGAATATTGCCATTATGCTTGACATGGTTTCCAAAAAATGGTACTCCACATACTTCA GTGAGGGTAAGTATTTTCCTGTTGTCAAGAATAGCATTGTAAAAGCATTTTGTAATAATA AAGAATAGCTTTAATGATATGCTTGTAACTAAAATAATTTTGTAATGTATCAAATACATT TAAAACATTAAAATATAATCTCTATAATAAAAAAAAAAAAAAA >OriGene 5' read for NM_004004 unedited
GAGTTANCATTTGTATACGACTTATATAGGCGGCCGCGACTTCCCGGGATATCGTCGACC CACGCGTCCGCGGAGCCCCTCGGCGGCGCCCGGCCCAGGACCCGCCTAGGAGCGCAGGAG CCCCAGCGCAGAGACCCCAACGCCGAGACCCCCGCCCCGGCCCCGCCGCGCTTCCTCCCG ACGCAGAGCAAACCGCCCAGAGTAGAAGATGGATTGGGGCACGCTGCAGACGATCCTGGG GGGTGTGAACAAACACTCCACCAGCATTGGAAAGATCTGGCTCACCGTCCTCTTCATTTT TCGCATTATGATCCTCGTTGTGGCTGCAAAGGAGGTGTGGGGAGATGAGCAGGCCGACTT TGTCTGCAACACCCTGCAGCCAGGCTGCAAGAACGTGTGCTACGATCACTACTTCCCCAT CTCCCACATCCGGCTATGGGCCCTGCAGCTGATCTTCGTGTCCACGCCAGCGCTCCTAGT GGCCATGCACGTGGCCTACCGGAGACATGAGAAGAAGAGGAAGTTCATCAAGGGGGAGAT AAAGAGTGAATTTAAGGACATCGAGGAGATCAAAACCCAGAAGGTCCGCATCGAAGGCTC CCTGTGGTGGACCTACACAAGCAGCATCTTCTTCCGGGTCATCTTCGAAGCCGCCTTCAT GTACGTCTTCTATGTCATGTACGACGGGCTCTCCATGCAGCGGCTGGTGAAGTGCAACGC CTGGCCTTGTNCCCAACACTGTGGACTGCTTTGTGTCCCGGCCACAGAGAAGACTGGTCT CACAGTGTTCATGATTGCAGTGTCTGGAATTTGCATCCTGCTGAATGTCACTGGATTGGG GTATTTGCTAATAAGAAATGGTCCTGGGGAGTCAAAAAAGCCAGTTTAACGCATTGCCCA GTTGGTAGAT |
| Restriction Sites | Please inquire |
| ACCN | NM_004004 |
| Insert Size | 2263 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004004.3, NP_003995.2 |
| RefSeq Size | 2263 bp |
| RefSeq ORF | 681 bp |
| Locus ID | 2706 |
| UniProt ID | P29033 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Gene Summary | This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq, Oct 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules
,Tong, X;Lopez, W;Ramachandran, J;Ayad, WA;Liu, Y;Lopez-Rodriguez, A;Harris, AL;Contreras, JE;,
J. Gen. Physiol.
,PubMed ID 26324677
[GJB2]
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Surdez em Portugal: estudos genéticos e funcionais do gene da conexina
,Assunção, JC;,
Thesis
[GJB2]
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|
Insights on the mechanisms of Ca2+ regulation of connexin26 hemichannels revealed by human pathogenic mutations (D50N/Y)
,William Lopez, Jorge Gonzalez, Yu Liu, Andrew L. Harris, and Jorge E. Contreras,
J. Gen. Physiol., Jun 2013; 142: 23 - 35.
,PubMed ID 23797420
[GJB2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202092 | GJB2 (Myc-DDK-tagged)-Human gap junction protein, beta 2, 26kDa (GJB2) |
CNY 2,400.00 |
|
| RC202092L1 | Lenti ORF clone of Human gap junction protein, beta 2, 26kDa (GJB2), Myc-DDK-tagged |
CNY 4,800.00 |
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| RC202092L2 | Lenti ORF clone of Human gap junction protein, beta 2, 26kDa (GJB2), mGFP tagged |
CNY 5,890.00 |
|
| RC202092L3 | Lenti ORF clone of Human gap junction protein, beta 2, 26kDa (GJB2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202092L4 | Lenti ORF clone of Human gap junction protein, beta 2, 26kDa (GJB2), mGFP tagged |
CNY 5,890.00 |
|
| RG202092 | GJB2 (tGFP-tagged) - Human gap junction protein, beta 2, 26kDa (GJB2) |
CNY 4,000.00 |
