Rad51L1 (RAD51B) (BC030219) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DNA repair protein RAD51 homolog 2; hREC2; MGC34245; R51H2; RAD51 homolog B; RAD51-like 1; RAD51-like 1 (S. cerevisiae); RAD51B; REC2; RecA-like protein; recombination repair protein
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC030219 edited GGGGAAACTTGAAAGTTGGATGCTGCAGACCCGGCATGGGTAGCAAGAAACTAAAACGAG TGGGTTTATCACAAGAGCTGTGTGACCGTCTGAGTAGACATCAGATCCTTACCTGTCAGG ACTTTTTATGTCTTTCCCCACTGGAGCTTATGAAGGTGACTGGTCTGAGTTATCGAGGTG TCCATGAACTTCTATGTATGGTCAGCAGGGCCTGTGCCCCAAAGATGCAAACGGCTTATG GGATAAAAGCACAAAGGTCTGCTGATTTCTCACCAGCATTCTTATCTACTACCCTTTCTG CTTTGGACGAAGCCCTGCATGGTGGTGTGGCTTGTGGATCCCTCACAGAGATTACAGGTC CACCAGGTTGTGGAAAAACTCAGTTTTGTATAATGATGAGCATTTTGGCTACATTACCCA CCAACATGGGAGGATTAGAAGGAGCTGTGGTGTACATTGACACAGAGTCTGCATTTAGTG CTGAAAGACTGGTTGAAATAGCAGAATCCCGTTTTCCCAGATATTTTAACACTGAAGAAA AGTTACTTTTGACAAGTAGTAAAGTTCATCTTTATCGGGAACTCACCTGTGATGAAGTTC TACAAAGGATTGAATCTTTGGAAGAAGAAATTATCTCAAAAGGAATTAAACTTGTGATTC TTGACTCTGTTGCTTCTGTGGTCAGAAAGGAGTTTGATGCACAACTTCAAGGCAATCTCA AAGAAAGAAACAAGTTCTTGGCAAGAGAGGCATCCTCCTTGAAGTATTTGGCTGAGGAGT TTTCAATCCCAGTTATCTTGACGAATCAGATTACAACCCATCTGAGTGGAGCCCTGGCTT CTCAGGCAGACCTGGTGTCTCCAGCTGATGATTTGTCCCTGTCTGAAGGCACTTCTGGAT CCAGCTGTGTGATAGCCGCACTAGGAAATACCTGGAGTCACAGTGTGAATACCCGGCTGA TCCTCCAGTACCTTGATTCAGAGAGAAGACAGATTCTTATTGCCAAGTCCCCTCTGGCTC CCTTCACCTCATTTGTCTACACCATCAAGGAGGAAGGCCTGGTTCTTCAAGAGACAACAT TTTGCTCTGTCACCCAAGCTGAACTGAACTGGGCTCCAGAAATCCTCCCACCTCAGCCTC CTGAGCAGCTAGGACTACAGATGTGCCACCATACCCAGCTAATTTTTTAATTTTTTGTAG AGGTGGGGTCTCATTATGTTGCCCAAGCTGATCTCAAACTCCTGGCTTCAAGAGATCCAC CCACCTCAGCCTCCCAAAGCGCTAGGATTACAAGTATGAGACTTTGCCATTCCAATGAGA ATTTTGTCCTTAGCAATCTCATCCAATGGCCACTGGAGATGTGTGTAGGGCCACAAGAAT GAGAAAGGGGGCAATGGAGTGACAGGTGACCTGCTCAGGTGCCCCTCCCCAGACCAAATG TCCCAGGCACACAGCCCATGCCCTCCACCATGGAGTACATTTTCTCCCTGGCTTGCTGCT GAGCTAAAGGACCAGGGACGTGGAAACAGGAGGTGAACTAAGGCACCCCGCCACCCACTG TGGCAGCTTGAATTTCTTGAGGCTGAGACAAACTAGGATCCAGTAGCCTAGATTTTGCCC CAGTTTCTCCACTGAGTATTTACTCAGTGCTCCTGAGCCGATGCCTTCCCCTCTGCCTAG ATGAACCACAGCATTTTGGTAAATGCCTCAGCATGTTAGGAGCGCTGGAACGTTTATTCT CTTCCCTCCCAATTATCCACTCTAATGGAGCAGAACACTTACATGGAAAATCCTAAACAT GGAAGATGATTTAAAAATGATTCACCCTGGAATACATGCTTCCAGTTGCTTCTACAGGTT TACCTTGCTAAAGGCATTTCGCTTCCTTTGCTGTTACAATAAGCTTGCCTGCCTTGAAGA AAAGAAGACCAGGGCCAAAGGGAAATCAGTCAAGGGAGCCTGTTTTGTCTGAAATAATGC ATCTATGAACAAATGAATTGAGTATAACTGTCTTTTTACATCAGTGTTTCTGTGGAATTA AGAGAACATCCAAGGCCACTCCTAGCTTGAGTAGATTAAATTTGTCCTTGTGTTTGGAGC AAGGGTTGTGAAGTCTGTCTGGCCCATCAAATGACCAATGGCTTTTAAGGAAGGACTAAG TGACTTATGACCAGAACAAGGGAGCAGCCTGAGTCAGTCTCCCCAGTGTCTACATTGTGT TATTTATACTAGCAAAAAAATAGACACAACTAAATGTTCGACCATATGGCATTGGTTAAA TTAACATCTATACGATGGAATACTAGATGGTCATTAAAATTATGTGTCAGAAGAAGGCTT AATCTCATGGGAAAGTGTTCACACCATATTATTAAAGAGAAAAAACAGATTATAAAGCAA TATGCATACAATGTAGTAGTTCTGTAAGAAAAAAGTATTATGTGCATTTTAACTGGAAAA ATATGTATAAAAATGTTAACAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC030219 unedited GACTCTGGTTTTGTAATACGACTTTCTATAGGCGGCCGCGCATTCCCGGGCTGGGGAAAC TTGAAAGTTGGATGCTGCAGACCCGGCATGGGTAGCAAGAAACTAAAACGAGTGGGTTTA TCACAAGAGCTGTGTGACCGTCTGAGTAGACATCAGATCCTTACCTGTCAGGACTTTTTA TGTCTTTCCCCACTGGAGCTTATGAAGGTGACTGGTCTGAGTTATCGAGGTGTCCATGAA CTTCTATGTATGGTCAGCAGGGCCTGTGCCCCAAAGATGCAAACGGCTTATGGGATAAAA GCACAAAGGTCTGCTGATTTCTCACCAGCATTCTTATCTACTACCCTTTCTGCTTTGGAC GAAGCCCTGCATGGTGGTGTGGCTTGTGGATCCCTCACAGAGATTACAGGTCCACCAGGT TGTGGAAAAACTCAGTTTTGTATAATGATGAGCATTTTGGCTACATTACCCACCAACATG GGAGGATTAGAAGGAGCTGTGGTGTACATTGACACAGAGTCTGCATTTAGTGCTGAAAGA CTGGTTGAAATAGCAGAATCCCGTTTTCCCAGATATTTTAACACTGAAGAAAAGTTACTT TTGACAAGTAGTAAAGTTCATCTTTATCGGGAACTCACCTGTGATGAAGTTCTACAAAGG ATTGAATCTTTGGAAGAAGAAATTATCTCANAAGAATTAAACTTGTGATTCTTGACTCTG TTGCTTCTGTGGTCAGAAAGGAGTTTGATGCACAACTTCAAGGGCATCTCAAAGAAAGAA AACAGTTNCTGGCAAGAGAAGCATCCTCCTTGAAGTATTTGGGCTGAGAGTTTTCATCCC AGTTATCTTGACGAATCAGATACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGAC CTGGTGTCTTCAGCTGATGATTTGTCCCTGTCTG
Restriction Sites	Please inquire
ACCN	BC030219
Insert Size	2524 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC030219.1, AAH30219.1
RefSeq Size	2524 bp
Locus ID	5890
Protein Families	Druggable Genome
Protein Pathways	Homologous recombination
Gene Summary	The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata. [provided by RefSeq, Mar 2016]

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