APOBEC3F (BC017022) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A3D; APOBEC3DE; APOBEC3E; ARP6
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC017022 edited CTGGGACAAGCGTATCTAAGAGGCTGAACATGAATCCACAGATCAGAAATCCGATGGAGC GGATGTATCGAGACACATTCTACGACAACTTTGAAAACGAACCCATCCTCTATGGTCGGA GCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGCTCAAATCTCCTTTGGG ACACAGGGGTCTTTCGAGGCCCGGTACTACCCAAACGTCAGTCGAATCACAGGCAGGAGG TGTATTTCCGGTTTGAGAACCACGCAGAAATGTGCTTCTTATCTTGGTTCTGTGGCAACC GACTGCCTGCTAACAGGCGCTTCCAGATCACCTGGTTTGTATCATGGAACCCCTGCCTGC CCTGTGTGGTGAAGGTGACCAAATTCTTGGCTGAGCACCCCAATGTCACCCTGACCATCT CTGCCGCCCGCCTCTACTACTACCGGGATAGAGATTGGCGGTGGGTGCTCCTCAGGCTGC ATAAGGCAGGGGCCCGTGTGAAGATCATGGACTATGAAGACTTTGCATACTGCTGGGAAA ACTTTGTGTGCAATGAAGGTCAGCCATTCATGCCTTGGTACAAATTCGATGACAATTATG CATCCCTGCACCGCACGCTAAAGGAGATTCTCAGAAACCCGATGGAGGCAATGTACCCAC ACATATTCTACTTCCACTTTAAAAACCTACTGAAAGCCTGTGGTCGGAACGAAAGCTGGC TGTGCTTCACCATGGAAGTTACAAAGCACCACTCAGCTGTCTTCCGGAAGAGGGGCGTCT TCCGAAACCAGGTGGATCCTGAGACCCATTGTCATGCAGAAAGGTGCTTCCTCTCTTGGT TCTGTGACGACATACTGTCTCCTAACACAAACTACGAGGTCACCTGGTACACATCTTGGA GCCCTTGCCCAGAGTGTGCAGGGGAGGTGGCCGAGTTCCTGGCCAGGCACAGCAACGTGA ATCTCACCATCTTCACCGCCCGCCTCTGCTATTTCTGGGATACAGATTACCAGGAGGGGC TCTGCAGCCTGAGTCAGGAAGGGGCCTCCGTGAAGATCATGGGCTACAAAGATTTTGTAT CTTGTTGGAAAAACTTTGTGTACAGTGATGATGAGCCATTCAAGCCTTGGAAGGGACTAC AAACCAACTTTCGACTTCTGAAAAGAAGGCTACGGGAGATTCTCCAGTGAGGGGTCTCCC TGGGCCTCATGGTCTGTCTCTTCTAGCCTCCTGCTCATGCTGCACGGGCCTCCCCTCCAT CCTGCACCAGCTGTGCTTTTGCCTGGTCATCCTGAGCCCCTCCTGGCCTCAGGGCCATTC CATAGTGCCCCCCTGCCTCACCACCTCCTCCCCGCTCTCCCAGGCTCTTCTTGTAGAGGC TCTCCATCCACCTCCCCAGTCCTGTTCCCCCAGCCTGGGTGCCCCTAACTTGACTCTTCC CATCTCCCCAGCATAACCAAATCTTTTTTTTTTTTTTTTTGAGACGGAGTTTCACTCTGT CGCCCAGACTAGAGTGCAATGGCTGGATCTCAGCTCACTGCAAACTCTGCTTACTGGGTT CAAGTGATTCTCCTGTCTCAGCTTCTGAGTGGCTGGGATTACAGATGCCTGCCACCACGC CCAGCTAATTTTTTTTTTTTTTTGTATTTTTAGTAGTGACTGGGTTTCACCATGTTGGCC AGGCTGGTCTTGAACTCCTGACCTCGGGTGATCTGCCCATCTCTGTCTCCCAAAGTGCTG GGATTACGGGCGTGAGCCACCCGGCTCGGCTGCACAACCAAATCTTATTAAACTCAATCT AGTCTGGCCCTGGTGACTCACGCCTTTCGGAGGCAGAGGTGGGAGAATCGCTTGAGCCCA GAAGTTTGAGACCAGCCTGGGCCACACGACAAAGCCCCATTTCTACAAAAAAAAATGCAA AAAGAAAGCCAGATGTGGTGGCATGCACCTGTAGTTTAAGCTACTTGGGAGGATGAAGTG GGAGGACTGCTTGAGCCGGGGAGGTGGAGGCTGGAGTAAACTGAGATCGCGCCACAGAAC TCCAGTTTGAGCAACAGATCGAGACCCTGCCTGAAAATAAATCAATAAATACACTCAACC TAAATGGAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017022 unedited CCCCCGCAGTTCGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATCCCGGGAT ATCGTCGACCCACGCGTCCGCTGGGACAAGCGTATCTAAGAGGCTGAACATGAATCCACA GATCAGAAATCCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAAAACGA ACCCATCCTCTATGGTCGGAGCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGG CCGCTCAAATCTCCTTTGGGACACAGGGGTCTTTCGAGGCCCGGTACTACCCAAACGTCA GTCGAATCACAGGCAGGAGGTGTATTTCCGGTTTGAGAACCACGCAGAAATGTGCTTCTT ATCTTGGTTCTGTGGCAACCGACTGCCTGCTAACAGGCGCTTCCAGATCACCTGGTTTGT ATCATGGAACCCCTGCCTGCCCTGTGTGGTGAAGGTGACCAAATTCTTGGCTGAGCACCC CAATGTCACCCTGACCATCTCTGCCGCCCGCCTCTACTACTACCGGGATAGAGATTGGCG GTGGGTGCTCCTCAGGCTGCATAAGGCAGGGGCCCGTGTGAAGATCATGGACTATGAAGA CTTTGCATACTGCTGGGAAAACTTTGTGTGCAATGAAGGTCAGCCATTCATGCCTTGGTA CAAATTCNATGACAATTATGCATCCCTGCACCGCACGCTAAAGGAGATTCTCAAAAACCC GATGGAGGGCATGGACCCACACATATTCTACTTCCACTTTAAAAACCTACTGAAAGCCTG TGGTCGGAACGAAAGCTGGCTTGGGCTTCCCCCTGGGAGTTACCAAGCACNCCCTCAACT GTTTTTCGGAAGAGGGGCCGTCTTCCCAAACCAGGTGGATCCTGC
Restriction Sites	Please inquire
ACCN	BC017022
Insert Size	2124 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC017022.1
RefSeq Size	2124 bp
Locus ID	200316
Gene Summary	This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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