HLA (BC001023) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DRB1; DRw10; HLA-DR1B; HLA-DR3B; HLA-DRB; SS1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for BC001023 edited GTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGC ATGGTGTGTCTGAAGCTCCCTGGAGGCTCCTGCATGGCAGCTCTGACAGTGACACTGATG GTGCTGAGCTCCCCACTGGCTTTGGCTGGGGACACCCAACCACGTTTCCTGTGGCAGGGT AAGTATAAGTGTCATTTCTTCAACGGGACGGAGCGGGTGCAGTTCCTGGAAAGACTCTTC TATAACCAGGAGGAGTTCGTGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGACG GAGCTAGGGCGGCCTGTCGCCGAGTCCTGGAACAGCCAGAAGGACATCCTGGAGGACAGG CGGGGCCAGGTGGACACCGTGTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTG CAGCGGCGAGTCCATCCTGAGGTGACTGTGTATCCTGCCAAGACTCAGCCCCTGCAGCAC CACAACCTCCTGGTCTGCTCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGG TTCCGGAACGGCCAGGAAGAGAAGGCTGGGGTGGTGTCCACAGGCCTGATCCAGAATGGA GACTGGACCTTCCAGACCCTGGTGATGCTGGAAACAGTTCCTCGGAGTGGAGAAGTTTAC ACCTGCCAAGTGGAGCACCCAAGTGTGATGAGCCCTCTCACAGTGGAATGGAGAGCACGG TCTGAATCTGCACAGAGCAAGATGCTGAGTGGAGTCGGGGGCTTTGTGCTGGGCCTGCTC TTCCTTGGGGCCGGGTTGTTCATCTACTTCAGGAATCAGAAAGGACACTCTGGACTTCAG CCAACAGGATTCCTGAGCTGAAGTGAAGATGACCACATTCAAGGAAAAACCTTCTGCCCC AGCTTTGCAGGATGAAACACTTCCCCGCTTGGCTCTCATTCTTCCACAAGAGAGACCTTT CTCCGGACCTGGTTGCTACTGGTTCAGCAACTCTGCAGAAAATGTCCTCCCCTGTGGCTG CCTCAGCTCATGCCTTTGGCCTGAAGTCCCAGCATTGATGGCAGCCCCTCATCTTCCAAG TTTTGTGCTCCCCTTTACCTAACGCTTCCTGCCTCCCATGCATCTGTACTCCTCCTGTGC CACAAACACATTACATTATTAAATGTTTCTCAAACATGGAAAAAAAAAAAAAAAAAAAAA AAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001023 unedited NNNAAGGTCAAAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTTCT CCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGT GTGTCTGAAGCTCCCTGGAGGCTCCTGCATGGCAGCTCTGACAGTGACACTGATGGTGCT GAGCTCCCCACTGGCTTTGGCTGGGGACACCCAACCACGTTTCCTGTGGCAGGGTAAGTA TAAGTGTCATTTCTTCAACGGGACGGAGCGGGTGCAGTTCCTGGAAAGACTCTTCTATAA CCAGGAGGAGTTCGTGCGCTTCGACAGCGACGTGGGGGAGTACCGGGCGGTGACGGAGCT AGGGCGGCCTGTCGCCGAGTCCTGGAACAGCCAGAAGGACATCCTGGAGGACAGGCGGGG CCAGGTGGACACCGTGTGCAGACACAACTACGGGGTTGGTGAGAGCTTCACAGTGCAGCG GCGAGTCCATCCTGAGGTGACTGTGTATCCTGCCAAGACTCAGCCCCTGCAGCACCACAA CCTCCTGGTCTGCTCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCG GAACGGCCAGGAAGAGAAGGCTGGGGTGGTGTCCACAGGCCTGATCCAGAATGGAGACTG GACCTTCCAGACCCTGGTGATGCTGGAAACAGTTCCTCGGAGTGGAGAAGTTTACACCTG CCAAGTGGAGCACCCAAGTGTGATGAGCCCTCTCACAGTGGAATGGAGAGCACGGTCTGA ATCTGCACAGAGCAAGATGCTGAGTGGAGTCGGGGGCTTTGTGCTGGGCCTGCTCTTCCT GGGGCCGGGTTGGTCATCTACTTCAGGAATCAGAAAGACACTCTGGACTTCACCAACAGG ATCCTGAGCTG
Restriction Sites	Please inquire
ACCN	BC001023
Insert Size	1212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001023.2, AAH01023.1
RefSeq Size	1203 bp
RefSeq ORF	798 bp
Locus ID	3125
Protein Families	Transmembrane
Protein Pathways	Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
Gene Summary	HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene. [provided by RefSeq, Feb 2020]

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