United States
Germany
Japan
United Kingdom
China
NGF (NM_002506) Human Untagged Clone
CAT＃: SC123827
NGF (untagged)-Human nerve growth factor (beta polypeptide) (NGF)
CNY 3,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Beta-NGF; HSAN5; NGFB |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_002506 edited
CCACGCGTCCGCGGACGCGTGGGCGCTGGGAGCCGGAGGGGAGCGCAGCGAGTTTTGGCC AGTGGTCGTGCAGTCCAAGGGGCTGGATGGCATGCTGGACCCAAGCTCAGCTCAGCGTCC GGACCCAATAACAGTTTTACCAAGGGAGCAGCTTTCTATCCTGGCCACACTGAGGTGCAT AGCGTAATGTCCATGTTGTTCTACACTCTGATCACAGCTTTTCTGATCGGCATACAGGCG GAACCACACTCAGAGAGCAATGTCCCTGCAGGACACACCATCCCCCAAGTCCACTGGACT AAACTTCAGCATTCCCTTGACACTGCCCTTCGCAGAGCCCGCAGCGCCCCGGCAGCGGCG ATAGCTGCACGCGTGGCGGGGCAGACCCGCAACATTACTGTGGACCCCAGGCTGTTTAAA AAGCGGCGACTCCGTTCACCCCGTGTGCTGTTTAGCACCCAGCCTCCCCGTGAAGCTGCA GACACTCAGGATCTGGACTTCGAGGTCGGTGGTGCTGCCCCCTTCAACAGGACTCACAGG AGCAAGCGGTCATCATCCCATCCCATCTTCCACAGGGGCGAATTCTCGGTGTGTGACAGT GTCAGCGTGTGGGTTGGGGATAAGACCACCGCCACAGACATCAAGGGCAAGGAGGTGATG GTGTTGGGAGAGGTGAACATTAACAACAGTGTATTCAAACAGTACTTTTTTGAGACCAAG TGCCGGGACCCAAATCCCGTTGACAGCGGGTGCCGGGGCATTGACTCAAAGCACTGGAAC TCATATTGTACCACGACTCACACCTTTGTCAAGGCGCTGACCATGGATGGCAAGCAGGCT GCCTGGCGGTTTATCCGGATAGATACGGCCTGTGTGTGTGTGCTCAGCAGGAAGGCTGTG AGAAGAGCCTGACCTGCCGACACGCTCCCTCCCCCTGCCCCTTCTACACTCTCCTGGGCC CCTCCCTACCTCAACCTGTAAATTATTTTAAATTATAAGGACTGCATGGTAATTTATAGT TTATACAGTTTTAAAGAATCATTATTTATTAAATTTTTGGAAGCAAAAAAAAAAAAAAA >OriGene 5' read for NM_002506 unedited
GGGGAGTTCACATTTGTATACGACTCATATAGGCGGCCGACGATTCATATCTGGTACCGG TCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCGGACGCGTGGGCGCTGGGAGCC GGAGGGGAGCGCAGCGAGTTTTGGCCAGTGGTCGTGCAGTCCAAGGGGCTGGATGGCATG CTGGACCCAAGCTCAGCTCAGCGTCCGGACCCAATAACAGTTTTACCAAGGGAGCAGCTT TCTATCCTGGCCACACTGAGGTGCATAGCGTAATGTCCATGTTGTTCTACACTCTGATCA CAGCTTTTCTGATCGGCATACAGGCGGAACCACACTCAGAGAGCAATGTCCCTGCAGGAC ACACCATCCCCCAAGTCCACTGGACTAAACTTCAGCATTCCCTTGACACTGCCCTTCGCA GAGCCCGCAGCGCCCCGGCAGCGGCGATAGCTGCACGCGTGGCGGGGCAGACCCGCAACA TTACTGTGGACCCCAGGCTGTTTAAAAAGCGGCGACTCCGTTCACCCCGTGTGCTGTTTA GCACCCAGCCTCCCCGTGAAGCTGCAGACACTCAGGATCTGGACTTCGAGGTCGGTGGTG CTGCCCCCTTCAACAGGACTCACAGGAGCAAGCGGTCATCATCCCATCCCATCTTCCACA GGGGCGAATTCTCGGTGTGTGACAGTGTCAGCGTGTGGGTTGGGGATAAGACCACCGCCA CAGACATCAAGGGCAAGGAGGTGATGGTGTTGGGAGAGGTGAACATTAACAACAGTGTAT TCAAACAGTACTTTTTTGAGACCAAGTGCCGGGACCCAAATCCCGTTGACAGCGGGTGCC GGNGCATTGACTCANACACTGGNACTCATATTGTACCACGACTCACACCTTTNGTCAGGC GCTGACCAG >OriGene 3' read for NM_002506 unedited
CATAGTCATGGCATGGCACTTGCCAGGGCCAGGANAGCACTGGGNGAGGGTCACAGGGAT GCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCCCTTTTTTTTTTTTTTT GCTTCCAAAAATTTAATAAATAATGATTCTTTAAAACTGTATAAACTATAAATTACCATG CAGTCCTTATAATTTAAAATAATTTACAGGTTGAGGTAGGGAGGGGCCCAGGAGAGTGTA AAAGGGGCAGGGGGAGGGAGCGTGTCGGCAGGTCAGGCTCTTCTCACAGCCTTCCTGCTG AGCACACACACACAGGCCGTATCTATCCGGATAAACCGCCAGGCAGCCTGCTTGCCATCC ATGGTCAGCGCCTTGACAAAGGTGTGAGTCGTGGTACAATATGAGTTCCAGTGCTTTGAG TCAATGCCCCGGCACCCGCTGTCAACGGGATTTGGGTCCCGGCACTTGGTCTCAAAAAAG TACTGTTTGAATACACTGTTGTTAATGTTCACCTCTCCCAACACCATCACCTCCTTGCCC TTGATGTCTGTGGCGGTGGTCTTATCCCCAACCCACACGCTGACACTGTCACACACCGAG AATTCNCCCCTGTGGAAGATGGGATGGGATGATGACCGCTTGCTCCTGTGAGTCCTGTTG AAGGGGGCAGCACCACCGACCTCGAAGTCCAGATCCTGAGTGTCTGCAGCTTCACGGGGA GGCTGGGTGCTAAACAGCACACGGTGTGAACGGAGTCGCCGCTTTTTAAACAGCCTGGGG TCCACAGTAATGGTTGCGGGTCTGCCCCGCCACGCGTGCAACTTTCGCCGCTGCCGGGGC GCTCGGGCTCTTGAAAGGGCAGTGTCAAGGTATGCTTAAATTTAATCCGTGGACTGGGGG AGTGC |
| Restriction Sites | Please inquire |
| ACCN | NM_002506 |
| Insert Size | 1200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002506.2, NP_002497.2 |
| RefSeq Size | 1052 bp |
| RefSeq ORF | 726 bp |
| Locus ID | 4803 |
| UniProt ID | P01138 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Apoptosis, MAPK signaling pathway, Neurotrophin signaling pathway |
| Gene Summary | This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The NGFR100W mutation specifically impairs nociception without affecting cognitive performance in a mouse model of Hereditary Sensory and Autonomic Neuropathy type V
,Testa, G;Mainardi, M;Morelli, C;Olimpico, F;Pancrazi, L;Petrella, C;Severini, C;Florio, R;Malerba, F;Stefanov, A;Strettoi, E;Brandi, R;Arisi, I;Heppenstall, P;Costa, M;Capsoni, S;Cattaneo, A;,
J. Neurosci.
,PubMed ID 31685654
[NGF]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221463 | NGF (Myc-DDK-tagged)-Human nerve growth factor (beta polypeptide) (NGF) |
CNY 3,600.00 |
|
| RC221463L1 | Lenti ORF clone of Human nerve growth factor (beta polypeptide) (NGF), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC221463L2 | Lenti ORF clone of Human nerve growth factor (beta polypeptide) (NGF), mGFP tagged |
CNY 6,000.00 |
|
| RC221463L3 | Lenti ORF clone of Human nerve growth factor (beta polypeptide) (NGF), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221463L4 | Lenti ORF clone of Human nerve growth factor (beta polypeptide) (NGF), mGFP tagged |
CNY 6,000.00 |
|
| RG221463 | NGF (tGFP-tagged) - Human nerve growth factor (beta polypeptide) (NGF) |
CNY 5,200.00 |
