TRPA1 (NM_007332) Human Untagged Clone

Name: TRPA1 (NM_007332) Human Untagged Clone
SKU: SC124028
Price: 17440.00

CAT＃: SC124028

TRPA1 (untagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)

Datasheet

SDS

"NM_007332" in other vectors (6)

CNY 17,440.00

货期*

现货

规格

Cited in 14 publications.

Product images

经常一起买 (3)

TA328744

Rabbit Polyclonal Anti-TRPA1 (extracellular)

CNY 11,000.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANKTM1; FEPS; FEPS1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_007332 edited ATGAAGCGCAGCCTGAGGAAGATGTGGCGCCCTGGAGAAAAGAAGGAGCCCCAGGGCGTT GTCTATGAGGATGTGCCGGACGACACGGAGGATTTCAAGGAATCGCTTAAGGTGGTTTTT GAAGGAAGTGCATATGGATTACAAAACTTTAATAAGCAAAAGAAATTAAAAAGATGTGAC GATATGGACACCTTCTTCTTGCATTATGCTGCAGCAGAAGGCCAAATTGAGCTAATGGAG AAGATCACCAGAGATTCCTCTTTGGAAGTGCTGCATGAAATGGATGATTATGGAAATACC CCTCTGCATTGTGCTGTAGAAAAAAACCAAATTGAAAGCGTTAAGTTTCTTCTCAGCAGA GGAGCAAACCCAAACCTCCGAAACTTCAACATGATGGCTCCTCTCCACATAGCTGTGCAG GGCATGAATAATGAGGTGATGAAGGTCTTGCTTGAGCATAGAACTATTGATGTTAATTTG GAAGGAGAAAATGGAAACACAGCTGTGATCATTGCGTGCACCACAAATAATAGCGAAGCA TTGCAGATTTTGCTTAACAAAGGAGCTAAGCCATGTAAATCAAATAAATGGGGATGTTTC CCTATTCACCAAGCTGCATTTTCAGGTTCCAAAGAATGCATGGAAATAATACTAAGGTTT GGTGAAGAGCATGGGTACAGTAGACAGTTGCACATTAACTTTATGAATAATGGGAAAGCC ACCCCTCTCCACCTGGCTGTGCAAAATGGTGACTTGGAAATGATCAAAATGTGCCTGGAC AATGGTGCACAAATAGACCCAGTGGAGAAGGGAAGGTGCACAGCCATTCATTTTGCTGCC ACCCAGGGAGCCACTGAGATTGTTAAACTGATGATATCGTCCTATTCTGGTAGCGTGGAT ATTGTTAACACAACCGATGGATGTCATGAGACCATGCTTCACAGAGCTTCATTGTTTGAT CACCATGAGCTAGCAGACTATTTAATTTCAGTGGGAGCAGATATTAATAAGATCGATTCT GAAGGACGCTCTCCACTTATATTAGCAACTGCTTCTGCATCTTGGAATATTGTAAATTTG CTACTCTCTAAAGGTGCCCAAGTAGACATAAAAGATAATTTTGGACGTAATTTTCTGCAT TTAACTGTACAGCAACCTTATGGATTAAAAAATCTGCGACCTGAATTTATGCAGATGCAA CAGATCAAAGAGCTGGTAATGGATGAAGACAACGATGGGTGTACTCCTCTACATTATGCA TGTAGACAGGGGGGCCCTGGTTCTGTAAATAACCTACTTGGCTTTAATGTGTCCATTCAT TCCAAAAGCAAAGATAAGAAATCACCTCTGCATTTTGCAGCCAGTTATGGGCGTATCAAT ACCTGTCAGAGGCTCCTACAAGACATAAGTGATACGAGGCTTCTGAATGAAGGTGACCTT CATGGAATGACTCCTCTCCATCTGGCAGCAAAGAATGGACATGATAAAGTAGTTCAGCTT CTTCTGAAAAAAGGTGCATTGTTTCTCAGTGACCACAATGGCTGGACAGCTTTGCATCAT GCGTCCATGGGCGGGTACACTCAGACCATGAAGGTCATTCTTGATACTAATTTGAAGTGC ACAGATCGCTTGGATGAAGACGGGAACACTGCACTTCACTTTGCTGCAAGGGAAGGCCAT GCCAAAGCCGTTGCGCTTCTTCTGAGCCACAATGCTGACATAGTCCTGAACAAGCAGCAG GCCTCCTTTTTGCACCTTGCACTTCACAATAAGAGGAAGGAGGTTGTTCTTACGATCATC AGGAGCAAAAGATGGGATGAATGTCTTAAGATTTTCAGTCATAATTCTCCAGGCAATAAA TGTCCAATTACAGAAATGATAGAATACCTCCCTGAATGCATGAAGGTACTTTTAGATTTC TGCATGTTGCATTCCACAGAAGACAAGTCCTGCCGAGACTATTATATCGAGTATAATTTC AAATATCTTCAATGTCCATTAGAATTCACCAAAAAAACACCTACACAGGATGTTATATAT GAACCGCTTACAGCCCTCAACGCAATGGTACAAAATAACCGCATAGAGCTTCTCAATCAT CCTGTGTGTAAAGAATATTTACTCATGAAATGGTTGGCTTATGGATTTAGAGCTCATATG ATGAATTTAGGATCTTACTGTCTTGGTCTCATACCTATGACCATTCTCGTTGTCAATATA AAACCAGGAATGGCTTTCAACTCAACTGGCATCATCAATGAAACTAGTGATCATTCAGAA ATACTAGATACCACGAATTCATATCTAATAAAAACTTGTATGATTTTAGTGTTTTTATCA AGTATATTTGGGTATTGCAAAGAAGCGGGGCAAATTTTCCAACAGAAAAGGAATTATTTT ATGGATATAAGCAATGTTCTTGAATGGATTATCTACACGACGGGCATCATTTTTGTGCTG CCCTTGTTTGTTGAAATACCAGCTCATCTGCAGTGGCAATGTGGAGCAATTGCTGTTTAC TTCTATTGGATGAATTTCTTATTGTATCTTCAAAGATTTGAAAATTGTGGAATTTTTATT GTTATGTTGGAGGTAATTTTGAAAACTTTGTTGAGGTCTACAGTTGTATTTATCTTCCTT CTTCTGGCTTTTGGACTCAGCTTTTACATCCTCCTGAATTTACAGGATCCCTTCAGCTCT CCATTGCTTTCTATAATCCAGACCTTCAGCATGATGCTAGGAGATATCAATTATCGAGAG TCCTTCCTAGAACCATATCTGAGAAATGAATTGGCACATCCAGTTCTGTCCTTTGCACAA CTTGTTTCCTTCACAATATTTGTCCCAATTGTCCTCATGAATTTACTTATTGGTTTGGCA GTTGGCGACATTGCTGAGGTCCAGAAACATGCATCATTGAAGAGGATAGCTATGCAGGTG GAACTTCATACCAGCTTAGAGAAGAAGCTGCCACTTTGGTTTCTACGCAAAGTGGATCAG AAATCCACCATCGTGTATCCCAACAAACCCAGATCTGGTGGGATGTTATTCCATATATTC TGTTTTTTATTTTGCACTGGGGAAATAAGACAAGAAATACCAAATGCTGATAAATCTTTA GAAATGGAAATATTAAAGCAGAAATACCGGCTGAAGGATCTTACTTTTCTCCTGGAAAAA CAGCATGAGCTCATTAAACTGATCATTCAGAAGATGGAGATCATCTCTGAGACAGAGGAT GATGATAGCCATTGTTCTTTTCAAGACAGGTTTAAGAAAGAGCAGATGGAACAAAGGAAT AGCAGATGGAATACTGTGTTGAGAGCAGTCAAGGCAAAAACACACCATCTTGAGCCTTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_007332 unedited NNNGGGGGGTTCGACAATATTTGTNAATACGNATTCACTATAGGGCCGGCCGCGNAATTC GCACGAGGAAGGTGCCCGCGCGCGTGGTGAGCAGCTGCACCAGGTGGCGTCCGGGGTGGG GTCAATGAAGCGCAGCCTGAGGAAGATGTGGCGCCCTGGAGAAAAGAAGGAGCCCCAGGG CGTTGTCTATGAGGATGTGCCGGACGACACGGAGGATTTCAAGGAATCGCTTAAGGTGGT TTTTGAAGGAAGTGCATATGGATTACAAAACTTTAATAAGCAAAAGAAATTAAAAAGATG TGACGATATGGACACCTTCTTCTTGCATTATGCTGCAGCAGAAGGCCAAATTGAGCTAAT GGAGAAGATCACCAGAGATTCCTCTTTGGAAGTGCTGCATGAAATGGATGATTATGGAAA TACCCCTCTGCATTGTGCTGTAGAAAAAAACCAAATTGAAAGCGTTAAGTTTCTTCTCAG CAGAGGAGCAAACCCAAACCTCCGAAACTTCAACATGATGGCTCCTCTCCACATAGCTGT GCAGGGCATGATATAATGAGGTGATGAAGGTCTTGCTTGAGCATAGAACTATTGATGTTA ATTTGGAAGGAGAAAATGGAAACACAGCTGTGATCATTGCGTGCACCACANATAATAGCG AAGCATTGCAGATTTTGCTTAACANAGGAGCTAAGCCATGTAAATCAAATAAATGGGGAT TGTTTCCCTATTCACCAAGCTGCATTTTCAGGTTCAAAGAATGCATGGAAATAATACTAA GGTTTGGTGAAGAGCATGGGTTCAGTATACAGTTGCACATTAACTTTATGAATAATGGGA AAGCCACCCCTTTCCACTGGCAGTGCCAAATGTGGACTTGGA 3' Read Nucleotide Sequence (showhide) >Forward primer walk for NM_007332 unedited GAAACATGCATCATTGAAGAGGATAGCTATGCAGGTGGAACTTCATACCAGCTTAGAGAA GAAGCTGCCACTTTGGTTTCTACGCAAAGTGGATCAGAAATCCACCATCGTGTATCCCAA CAAACCCAGATCTGGTGGGATGTTATTCCATATATTCTGTTTTTTATTTTGCACTGGGGA AATAAGACAAGAAATACCAAATGCTGATAAATCTTTAGAAATGGAAATATTAAAGCAGAA ATACCGGCTGAAGGATCTTACTTTTCTCCTGGAAAAACAGCATGAGCTCATTAAACTGAT CATTCAGAAGATGGAGATCATCTCTGAGACAGAGGATGATGATAGCCATTGTTCTTTTCA AGACAGGTTTAAGAAAGAGCAGATGGAACAAAGGAATAGCAGATGGAATACTGTGTTGAG AGCAGTCAAGGCAAAAACACACCATCTTGAGCCTTAGCTCCTCAGACCTTCAGTGAGGCT TCTAATGGGGGGTGCATGACTTGCTGGTTCTAACTTTCAATTTAAAAAGAGTGAGGAAGA AGCAGAATGATTCATTTTGCTGCGTGTGAAATCATGGTTCCTGCATGCTGTATAAAAGTA AACCATCTTTTATCCTCTATTCATATTTTCTACCAATCACTATGTATTGGGGATATCTTT GCAGATATGTTCAAATTGGACTGGACTTTGATGAGATATAATCTCATTATTTGAATGGGT AGAAAATGAATTTTGCTAGACACN
Restriction Sites	NotI-NotI
ACCN	NM_007332
Insert Size	4000 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	The ORF of this clone has been fully sequenced and found to contain 1 SNPs compared with NM_007332.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007332.1, NP_015628.1
RefSeq Size	5190 bp
RefSeq ORF	5190 bp
Locus ID	8989
UniProt ID	O75762
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Gene Summary	The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (14)

The use of this cDNA Clones has been cited in the following citations:
Proximal C-Terminus Serves as a Signaling Hub for TRPA1 Channel Regulation via Its Interacting Molecules and Supramolecular Complexes ,Zimova, L;Barvikova, K;Macikova, L;Vyklicka, L;Sinica, V;Barvik, I;Vlachova, V;, Front Physiol ,PubMed ID 32226391 [TRPA1]
Human and Mouse TRPA1 Are Heat and Cold Sensors Differentially Tuned by Voltage ,Sinica, V;Zimova, L;Barvikova, K;Macikova, L;Barvik, I;Vlachova, V;, Cells ,PubMed ID 31878344 [TRPA1]
Putative interaction site for membrane phospholipids controls activation of TRPA1 channel at physiological membrane potentials ,Macikova, L;Sinica, V;Kadkova, A;Villette, S;Ciaccafava, A;Faherty, J;Lecomte, S;Alves, ID;Vlachova, V;, FEBS J. ,PubMed ID 31116904 [TRPA1]
Intracellular cavity of sensor domain controls allosteric gating of TRPA1 channel ,Zimova, L;Sinica, V;Kadkova, A;Vyklicka, L;Zima, V;Barvik, I;Vlachova, V;, Sci Signal ,PubMed ID 29363587 [TRPA1]
Acute exposure to high-induction electromagnetic field affects activity of model peripheral sensory neurons ,Prucha, J;Krusek, J;Dittert, I;Sinica, V;Kadkova, A;Vlachova, V;, J. Cell. Mol. Med. ,PubMed ID 29210178 [TRPA1]
The First Extracellular Linker Is Important for Several Aspects of the Gating Mechanism of Human TRPA1 Channel ,Marsakova, L;Barvik, I;Zima, V;Zimova, L;Vlachova, V;, Front Mol Neurosci ,PubMed ID 28197074 [TRPA1]
N-terminal tetrapeptide T/SPLH motifs contribute to multimodal activation of human TRPA1 channel ,Hynkova, A;Marsakova, L;Vaskova, J;Vlachova, V;, Sci Rep ,PubMed ID 27345869 [TRPA1]
Pinosylvin Inhibits TRPA1-Induced Calcium Influx In Vitro and TRPA1-Mediated Acute Paw Inflammation In Vivo ,Moilanen, LJ;Hämäläinen, M;Lehtimäki, L;Nieminen, RM;Muraki, K;Moilanen, E;, Basic Clin. Pharmacol. Toxicol. ,PubMed ID 26335783 [TRPA1]
Structural modeling and patch-clamp analysis of pain-related mutation TRPA1-N855S reveal inter-subunit salt bridges stabilizing the channel open state ,Zíma, V;Witschas, K;Hynkova, A;Zímová, L;Barvík, I;Vlachova, V;, Neuropharmacology ,PubMed ID 25724085 [TRPA1]
Urate crystal induced inflammation and joint pain are reduced in transient receptor potential ankyrin 1 deficient mice - potential role for transient receptor potential ankyrin 1 in gout ,Moilanen, LJ;Hämäläinen, M;Lehtimäki, L;Nieminen, RM;Moilanen, E;, PLoS ONE ,PubMed ID 25658427 [TRPA1]
Tramadol and Its Metabolite M1 Selectively Suppress Transient Receptor Potential Ankyrin 1 Activity, but Not Transient Receptor Potential Vanilloid 1 Activity ,Miyano, K;Minami, K;Yokoyama, T;Ohbuchi, K;Yamaguchi, T;Murakami, S;Shiraishi, S;Yamamoto, M;Matoba, M;Uezono, Y;, Anesth. Analg. ,PubMed ID 25642661 [TRPA1]
C-terminal Acidic Cluster Is Involved in Ca2+-induced Regulation of Human Transient Receptor Potential Ankyrin 1 Channel ,Sura L, Zíma V, Marsakova L, Hynkova A, Barvík I, Vlachova V., J. Biol. Chem., May 2012; 287: 18067 - 18077. [TRPA1]
Novel Menthol-Derived Cooling Compounds Activate Primary and Second-Order Trigeminal Sensory Neurons and Modulate Lingual Thermosensitivity ,Amanda H. Klein, Mirela Iodi Carstens, T. Scott McCluskey, Guillaume Blancher, Christopher T. Simons, Jay P. Slack, Stefan Furrer, and Earl Carstens, Chem Senses, Sep 2011; 36: 649 - 658. [TRPA1]
Artificial Sweeteners and Salts Producing a Metallic Taste Sensation Activate TRPV1 Receptors ,Celine E Riera, Horst Vogel, Sidney A Simon, and Johannes le Coutre, Am J Physiol Regul Integr Comp Physiol. 2007 Aug;293(2):R626-34. [TRPA1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC219290	TRPA1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,688.00
RC219290L1	Lenti-ORF clone of TRPA1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,088.00
RC219290L2	Lenti-ORF clone of TRPA1 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,260.00
RC219290L3	Lenti-ORF clone of TRPA1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,260.00
RC219290L4	Lenti-ORF clone of TRPA1 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 14,088.00
RG219290	TRPA1 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 13,288.00

其它TRPA1产品

Expression Plasmids

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CRISPR

RNAi

Proteins

Antibodies