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PAK4 (NM_005884) Human Untagged Clone
CAT＃: SC124043
PAK4 (untagged)-Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1
CNY 6,608.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124043 sequence for NM_005884 edited (data generated by NextGen Sequencing)
ATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGC GTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAG AGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGACCCCGCCTGCATCACC TCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGGG GCCCTCACGCTGCTGCTGGACGAGTTTGAGAACATGTCGGTGACACGCTCCAACTCCCTG CGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAGGAG CCGGCCACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCGGTTCGCCGGT CACAGCGAGGCGGGTGGCGGCAGTGGTGACAGGCGACGGGCGGGGCCAGAGAAGAGGCCC AAGTCTTCCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCTCCCGGGACAAACGCCCCCTC TCCGGGCCTGATGTCGGCACCCCCCAGCCTGCTGGTCTGGCCAGTGGGGCGAAACTGGCA GCTGGCCGGCCCTTTAACACCTACCCGAGGGCTGACACGGACCACCCATCCCGGGGTGCC CAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCTGGCCATCCCC CAGTCCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGAGGTGCCCCCAGCCCTGGA GTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTCCAGCCTGCACCCCCGCCGCC CCTGCTGTTCCTGGGCCCCCTGGCCCCCGCTCACCACAGCGGGAGCCACAGCGAGTATCC CATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGCGACCCCCGCTCCTAC CTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGCCACCGTG CGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACCTGCGCAAGCAGCAGAGGCGC GAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCACGAGAATGTGGTGGAG ATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGA GGCGCCCTCACCGACATCGTCACCCACACCAGGATGAACGAGGAGCAGATCGCGGCCGTG TGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCACCGGGAC ATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGGTGAAGCTGTCAGACTTTGGG TTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCTGGTCGGCACGCCCTAC TGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCG CTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCCCCCTACTTCAACGAGCCACCC CTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCTGCACAAG GTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGGTGCGAGACCCTGCCCAGCGG GCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGCAGGGCCGCCTGCCAGC ATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGA Clone variation with respect to NM_005884.3 >OriGene 5' read for NM_005884 unedited
NGTAACGTTCAGCATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGT GTGGGGATTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGG GAGGCGCGAGGGCGCGGAGTTCCAGGTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCG AGCCGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATC TCCGCGCCGTCCAACTTCGAGCACCGCGTGCACACGGGCTTCGACCAGCACGAGCAGAAG TTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAG CCCCTCGTCGACCCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTG CGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGACGAGTTTGAGAAC ATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCC CGCCAGGAAAATGGGATGCCAGAGGAGCCGGCCACCACGGCCAGAGGGGGCCCAGGGAAG GCAGGCAGCCGAGGCCGGTTCGCCGGTCACAGCGAGCCGGGTTGGCCGGCAGTGGTTGAC ATGCCGACGGGCCGGGGCCACGATAATAGCCCCAAGTTCTTCCAGGGGAGGGCTCAGGGT GGTCCCCCAGGAATCCCTCCCGGGACAAACCCCCCCCTTCTCCCGGCCTGGATGTTCGGC CCCCCCACAACCCGCCTGATCTGGCGCCAGTGGGGCTTAAACTGANAACCTGGGCTGGCC CTTTGAAAACCTTCCCCGAGGTGCTGACCACGGTCCACCCTTATCCGGGGGGCGCCCTGT GGTGTTCTTTTATTA >OriGene 3' read for NM_005884 unedited
GCATTCAACTTCCCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGCCCCCCTTTTTTTTTGGGT TTTTTTTTTTTTTTTTTACAAGACAATGTTTTAATTGTAAAACTAACTCGAGGCAGGGGT GGGCGGGCTGGGGCTGCGCTGACCGGGCAGGAACCTGGTTCTTCAGGCAGTGGTTCTGCC AGGGCCACCCCGCAGGACAGGGACCATCTGTCCCCCAATAAGGGCAGGGGCTAGAGTGTT ATAAAATGACAATATAAATAGACTTCTAGAAAAGAGGAGGCTGTCCAGGTGCAGTAGTCA TTTGCTGCAGGGGCTGGAGAGGGTGGGGGGCCCTGACCTGTCTCCCCATCCAGCCACAGA AAGGGGGCGCAGGGCTACGGGAAAAGGCCACTCTTCGGACATTCATGATCGACCCCCACA ATGGCTCANGGGGAGAGGGGGCTGGGTGTCANGCAAGGCGCCAGACACCCCTTGCGGGCT GGAGGACGGGGTGGCTGGACCTTTGCACACACACACACATGCACACTCACACGCGTGCAC ACACTCATACATGTTCACCTCTTAGTGTTCTCTCCACCCCCAGCGACCCCCACTGCAAAA CTTCCAGTGATGGGAGGGGCCGCTGCCCTTTCCTGGAGAACAGAAAAACATGGGGGGAAG GCCTTGTCCCCAAAAGGGTGGGGGTTCTCGGGAACCCTCGCTTGTGTTCAACCAAGTTCC TGGGGTTTTTTCTAAAAAATCCACCATATTCAAAAACTGGGGTTTCAATAATGAGGGGTC TCAATCTCCCCCACAAAGGAGGGTGGGTGCCACGCCGGAAATTCTTCCGGGCTTGGAGAA ATTGGAAGCCTGTCCCCCTATATGGCTTTAATGGGGCGGGGAGACCCCGGGGCTTT |
| Restriction Sites | ECoRI-NOT |
| ACCN | NM_005884 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005884.3, NP_005875.1 |
| RefSeq Size | 2838 bp |
| RefSeq ORF | 1776 bp |
| Locus ID | 10298 |
| UniProt ID | O96013 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway |
| Gene Summary | PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Pak4, a Novel Gab1 Binding Partner, Modulates Cell Migration and Invasion by the Met Receptor
,Grigorios N. Paliouras, Monica A. Naujokas, and Morag Park,
Mol. Cell. Biol., Jun 2009; 29: 3018 - 3032.
[PAK4]
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Cdc42- and Rac1-mediated endothelial lumen formation requires Pak2, Pak4 and Par3, and PKC-dependent signaling
,Wonshill Koh, Rachel D. Mahan, and George E. Davis,
J. Cell Sci. 2008; 121(7): p. 989-1001
[PAK4]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202302 | PAK4 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1 |
CNY 6,600.00 |
|
| RC202302L1 | Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1, Myc-DDK-tagged |
CNY 9,000.00 |
|
| RC202302L2 | Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1, mGFP tagged |
CNY 9,000.00 |
|
| RC202302L3 | Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202302L4 | Lenti ORF clone of Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1, mGFP tagged |
CNY 9,000.00 |
|
| RG202302 | PAK4 (tGFP-tagged) - Human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1 |
CNY 8,200.00 |
