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CD16 (FCGR3A) (NM_000569) Human Untagged Clone
CAT＃: SC124061
FCGR3A (untagged)-Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1
CNY 3,600.00
| Cited in 6 publications. |
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD16; CD16A; FCG3; FCGR3; FCGRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC124061 sequence for NM_000569 edited (data generated by NextGen Sequencing)
ATGGGTGGAGGGGCTGGGGAAAGGCTGTTTACTTCCTCCTGTCTAGTCGGTTTGGTCCCT TTAGGGCTCCGGATATCTTTGGTGACTTGTCCTCTCCAGTGTGGCATCATGTGGCAGCTG CTCCTCCCAACTGCTCTGCTACTTCTAGTTTCAGCTGGCATGCGGACTGAAGATCTCCCA AAGGCTGTGGTGTTCCTGGAGCCTCAATGGTACAGGGTGCTCGAGAAGGACAGTGTGACT CTGAAGTGCCAGGGAGCCTACTCCCCTGAGGACAATTCCACACAGTGGTTTCACAATGAG AGCCTCATCTCAAGCCAGGCCTCGAGCTACTTCATTGACGCTGCCACAGTCGACGACAGT GGAGAGTACAGGTGCCAGACAAACCTCTCCACCCTCAGTGACCCGGTGCAGCTAGAAGTC CATATCGGCTGGCTGTTGCTCCAGGCCCCTCGGTGGGTGTTCAAGGAGGAAGACCCTATT CACCTGAGGTGTCACAGCTGGAAGAACACTGCTCTGCATAAGGTCACATATTTACAGAAT GGCAAAGGCAGGAAGTATTTTCATCATAATTCTGACTTCTACATTCCAAAAGCCACACTC AAAGACAGCGGCTCCTACTTCTGCAGGGGGCTTGTTGGGAGTAAAAATGTGTCTTCAGAG ACTGTGAACATCACCATCACTCAAGGTTTGGCAGTGTCAACCATCTCATCATTCTTTCCA CCTGGGTACCAAGTCTCTTTCTGCTTGGTGATGGTACTCCTTTTTGCAGTGGACACAGGA CTATATTTCTCTGTGAAGACAAACATTCGAAGCTCAACAAGAGACTGGAAGGACCATAAA TTTAAATGGAGAAAGGACCCTCAAGACAAATGA Clone variation with respect to NM_000569.6 93 a=>t;634 t=>g >OriGene 5' read for NM_000569 unedited
NNCCCAACGCCTTCACGATTTGTGAAACGACTTCTATAGGCGGCACGCGATTCCCGGGCA GATTCTGTGTGTGTCCTCAGCAGCTCAGCCACAGACCTTTGAGGGAGTAAAGGGGGCAGA CCCACCCACCTTGCCTCCAGGCTCTTTCCTTCCTGGTCCTGTTCTATGGTGGGGCTCCCT TGCCAGACTTCAGACTGAGAAGTCAGATGAAGTTTCAAGAAAAGGAAATTGGTGGGTGAC AGAGATGGGTGGAGGGGCTGGGGAAAGGCTGTTTACTTCCTCCTGTCTAGTCAGTTTGGT CCCTTTAAGGCTCCGGATATCTTTGGTGACTTGTCCTCTCCAGTGTGGCATCATGTGGCA GCTGCTCCTCCCAACTGCTCTGCTACTTCTAGTTTCAGCTGGCATGCGGACTGAAGATCT CCCAAAGGCTGTGGTGTTCCTGGAGCCTCAATGGTACAGGGTGCTCGAGAAAGACAGTGT GACTCTGAAGTGCCAGGGAGCCTACTCCCCTGAGGACAATTCCACACAGTGGTTTCACAA TGAGAGCCTCATCTCAAGCCAGGCCTCGAGCTACTTCATTGACGCTGCCACAGTCGACGA CAGTGGAGAGTACAGGTGCCAGACAAACCTCTCCACCCTCAGTGACCCGGTGCAGCTAGA AGTCCATATCGGCTGGCTGTTGCTCCAGGCCCCTCGGTGGGTGTTCAAGGAGGAAGACCC TATTCACCTGAAGTGTCACAGCTGGAAGAACACTGCTCTGCATAAAGTCACATATTTACA AGATGGCAAAAGCAGGAAGTATTTTCATCATAATTCTGACTTCTACATTCCAAAAGCCAC ACTCAAAGACAGCGGCTCCTACTTCTGCAAGGGAGCTTGTTGGGAGTAAAAATGTGTCTT CAGAAACTGTGA >OriGene 3' read for NM_000569 unedited
NAGGGTCAAGGGCTGCCACCCGGGTATCTGTTCAAGAAAACAGCTATGACCGCGGCCGCA ATCTACAGCGGCCGCCCTCTTTTTTNTTTTTTATTTATTTTTTATTTTTTCTTTTTTTAT CATTCATATTTTATTACCATGGTTTTCCCATCTTCTATCTAAAAGTAACATGCAATTTTT TTTAAAATGCTTTATTGGAACCAAAAAATGTTGCGCTTAAAGCTTACAAAACAAAGACAG CTAAAGCTTTCTTTCATAAACAACAATTGTCTTCTCCATCCCCACCTTATTGGAACTGAC ATGATGAAAGATTTGAAAGTTTCATAACTTAACTCAGCGAAGCTCAGTAGTACATTTAGT ATTGGTTATACAACATTTGTTTAATAAATGCAATGAACAAAGCTACACAGGAATTATATA TTGAAGCAAAAAAAGTGGTTTTACAGTCCCTGCATTAACCTCTAATTCTTACTACCCTGG CCAAAAAAGCATTTTCACCTCCTGCGCTTTCCTTCCTGTGTGCTTGAGGTTGGTTCTTTC TTCTCAAGCTTTCTCATTCTGATGCTGAGATAGTTCTGTTCACTTAGCAACTTGGGACAG AGACACAAGGTTTGTTCTGTACTTTCTTTTCCACCCCACCCCCCACCGAAATCCTCAATC CCCTTCCTAAGACCATTTTCTACTCCTAACATTAAAAAGACTCATCGTTATATACTTGAG AAATGGTCCAATTCTTATAAAATCCCCCTGGAAGACTCTATTTCTACCTCCCCCCCATGA ATTTCCTGGTAATTCCCCCTACGGTTATAGTTTTAAGAAAACACCCTTTTTTCTGGGGCT AAGATGGAAACCACCCCCCTTAAACTATCCTGGACT |
| Restriction Sites | Please inquire |
| ACCN | NM_000569 |
| Insert Size | 873 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000569.6, NP_000560.5 |
| RefSeq Size | 2406 bp |
| RefSeq ORF | 873 bp |
| Locus ID | 2214 |
| UniProt ID | P08637 |
| Domains | ig, IG |
| Protein Families | ES Cell Differentiation/IPS, Secreted Protein, Transmembrane |
| Protein Pathways | Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, Systemic lupus erythematosus |
| Gene Summary | This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] Transcript Variant: This variant (1) retains an intron in its 5' UTR and 5' coding region compared to variant 3. The encoded isoform (a) has a longer and distinct N-terminus compared to isoform c. This isoform (a) lacks a predicted signal peptide compared to isoform c. |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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HCMV-Specific Antibody Response And Development Of ADCC Against HCMV After Lung Transplantation
,Vietzen, H;Görzer, I;Honsig, C;Jaksch, P;Puchhammer-Stöckl, E;,
J. Infect. Dis.
,PubMed ID 32157310
[FCGR3A]
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Development of a simple new flow cytometric antibody-dependent cellular cytotoxicity (ADCC) assay with excellent sensitivity
,Tanaka, M;Ishige, A;Yaguchi, M;Matsumoto, T;Shirouzu, M;Yokoyama, S;Ishikawa, F;Kitabayashi, I;Takemori, T;Harada, M;,
J. Immunol. Methods
,PubMed ID 30389576
[FCGR3A]
|
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Development of a Cell-Based Assay Measuring the Activation of Fc?RIIa for the Characterization of Therapeutic Monoclonal Antibodies
,Tada, M;Ishii-Watabe, A;Suzuki, T;Kawasaki, N;,
PLoS ONE e95787 9 4. April 2014
,PubMed ID 24752341
[FCGR3A]
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T Lymphocytes Expressing a CD16 Signaling Receptor Exert Antibody-Dependent Cancer Cell Killing
,Kudo, K;Imai, C;Lorenzini, P;Kamiya, T;Kono, K;Davidoff, AM;Chng, WJ;Campana, D;,
Cancer Res.
,PubMed ID 24197131
[FCGR3A]
|
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CD16 Regulates TRIF-Dependent TLR4 Response in Human Monocytes and Their Subsets
,Irina N. Shalova, Tasneem Kajiji, Jyue Yuan Lim, Vanesa Gómez-Piña, Irene Fernández-Ruíz, Francisco Arnalich, Philip Tsau Choong Iau, Eduardo López-Collazo, Siew-Cheng Wong, and Subhra K. Biswas,
J. Immunol., Apr 2012; 188: 3584 - 3593.
[FCGR3A]
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TGF-ß Utilizes SMAD3 to Inhibit CD16-Mediated IFN- Production and Antibody-Dependent Cellular Cytotoxicity in Human NK Cells
,Rossana Trotta, Jessica Dal Col, Jianhua Yu, David Ciarlariello, Brittany Thomas, Xiaoli Zhang, Jeffrey Allard, II, Min Wei, Hsiaoyin Mao, John C. Byrd, Danilo Perrotti, and Michael A. Caligiuri,
J. Immunol., Sep 2008; 181: 3784 - 3792
[FCGR3A]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206429 | FCGR3A (Myc-DDK-tagged)-Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1 |
CNY 2,400.00 |
|
| RC206429L1 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC206429L2 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RC206429L3 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC206429L4 | Lenti ORF clone of Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
| RG206429 | FCGR3A (tGFP-tagged) - Human Fc fragment of IgG, low affinity IIIa, receptor (CD16a) (FCGR3A), transcript variant 1 |
CNY 4,000.00 |
