Nicotinic Acetylcholine Receptor alpha 7 (CHRNA7) (NM_000746) Human Untagged Clone
CAT#: SC124074
CHRNA7 (untagged)-Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1
CNY 5,616.00
Cited in 3 publications. |
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CHRNA7-2; NACHRA7 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_000746, the custom clone sequence may differ by one or more nucleotides
ATGCGCTGCTCGCCGGGAGGCGTCTGGCTGGCGCTGGCCGCGTCGCTCCTGCACGTGTCCCTGCAAGGCG AGTTCCAGAGGAAGCTTTACAAGGAGCTGGTCAAGAACTACAATCCCTTGGAGAGGCCCGTGGCCAATGA CTCGCAACCACTCACCGTCTACTTCTCCCTGAGCCTCCTGCAGATCATGGACGTGGATGAGAAGAACCAA GTTTTAACCACCAACATTTGGCTGCAAATGTCTTGGACAGATCACTATTTACAGTGGAATGTGTCAGAAT ATCCAGGGGTGAAGACTGTTCGTTTCCCAGATGGCCAGATTTGGAAACCAGACATTCTTCTCTATAACAG TGCTGATGAGCGCTTTGACGCCACATTCCACACTAACGTGTTGGTGAATTCTTCTGGGCATTGCCAGTAC CTGCCTCCAGGCATATTCAAGAGTTCCTGCTACATCGATGTACGCTGGTTTCCCTTTGATGTGCAGCACT GCAAACTGAAGTTTGGGTCCTGGTCTTACGGAGGCTGGTCCTTGGATCTGCAGATGCAGGAGGCAGATAT CAGTGGCTATATCCCCAATGGAGAATGGGACCTAGTGGGAATCCCCGGCAAGAGGAGTGAAAGGTTCTAT GAGTGCTGCAAAGAGCCCTACCCCGATGTCACCTTCACAGTGACCATGCGCCGCAGGACGCTCTACTATG GCCTCAACCTGCTGATCCCCTGTGTGCTCATCTCCGCCCTCGCCCTGCTGGTGTTCCTGCTTCCTGCAGA TTCCGGGGAGAAGATTTCCCTGGGGATAACAGTCTTACTCTCTCTTACCGTCTTCATGCTGCTCGTGGCT GAGATCATGCCCGCAACATCCGATTCGGTACCATTGATAGCCCAGTACTTCGCCAGCACCATGATCATCG TGGGCCTCTCGGTGGTGGTGACAGTGATCGTGCTGCAGTACCACCACCACGACCCCGACGGGGGCAAGAT GCCCAAGTGGACCAGAGTCATCCTTCTGAACTGGTGCGCGTGGTTCCTGCGAATGAAGAGGCCCGGGGAG GACAAGGTGCGCCCGGCCTGCCAGCACAAGCAGCGGCGCTGCAGCCTGGCCAGTGTGGAGATGAGCGCCG TGGCGCCGCCGCCCGCCAGCAACGGGAACCTGCTGTACATCGGCTTCCGCGGCCTGGACGGCGTGCACTG TGTCCCGACCCCCGACTCTGGGGTAGTGTGTGGCCGCATGGCCTGCTCCCCCACGCACGATGAGCACCTC CTGCACGGCGGGCAACCCCCCGAGGGGGACCCGGACTTGGCCAAGATCCTGGAGGAGGTCCGCTACATTG CCAACCGCTTCCGCTGCCAGGACGAAAGCGAGGCGGTCTGCAGCGAGTGGAAGTTCGCCGCCTGTGTGGT GGACCGCCTGTGCCTCATGGCCTTCTCGGTCTTCACCATCATCTGCACCATCGGCATCCTGATGTCGGCT CCCAACTTCGTGGAGGCCGTGTCCAAAGACTTTGCGTAA >OriGene 5' read for NM_000746 unedited
TAAAGTCAGATTTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGCGA GGTGCCTCTGTGGCCGCAGGCGCAGGCCCGGGCGACAGCCGAGACGTGGAGCGCGCCGGC TCGCTGCAGCTCCGGGACTCAACATGCGCTGCTCGCCGGGAGGCGTCTGGCTGGCGCTGG CCGCGTCGCTCCTGCACGTGTCCCTGCAAGGCGAGTTCCAGAGGAAGCTTTACAAGGAGC TGGTCAAGAACTACAATCCCTTGGAGAGGCCCGTGGCCAATGACTCGCAACCACTCACCG TCTACTTCTCCCTGAGCCTCCTGCAGATCATGGACGTGGATGAAAAGAACCAAGTTTTAA CCACCAACATTTGGCTGCAAATGTCTTGGACAGATCACTATTTACAGTGGAATGTGTCAT AATATCCAGGGGTGAAGACTGTTCGTTTCCCAGATGGCCACATTTGGAAACCAGACATTC TTCTCTATAACAGTGCTGATGAGCGCTTTGACGCCACATTCCACACTAACGTGTTGGTGA ATTCTTCTGGGCATTGCCAGTACCTGCCTCCAGGCATATTCAAGAGTTCCTGCTACATCG ATGTACGCTGGTTTCCCTTTGATGTGCAGCACTGCATACTGAAGTTTGGGTCCTGGTCTT ACGGACGCTGGCCCTTGGATCTGCAGATGCAGGGAGCAGATATCACTGGCTATATCCGCA AGGGAAAATGGGACCTAGTGGGAATCCCCCGCAAGAGGAGTGAAAGGTTATATGAGTGCT GCAAAGAACCCTACCCCGATGTCAACTTTACAGTGACCATGCGCAAAAGGACGCTCCACT ATTGGGCTCAACGGCTGATCCCCTGGGGGAAAAATTTCCACCCTGCGCTG >OriGene 3' read for NM_000746 unedited
CATGGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT GTTTTTTTTAACGGAGAAACAGCATCAAGCTGTTTCTCTCTACCGTCTTTGATAGAAATA AAAATAAAAATAAAAAGTTGAATTGCAGAAAAGCTAAGAGGTTTTTAGTTTTTGTTTTTT GTTTTCCTTCCACCAGTCAATTATTGGAAAGGATTTAGTGAGTCTGGTTTATTTTAGCTT CAATCTGGGTTTGTACACAAGCAAAAAGCAAATGTTGAATTTTCAAGTAGACCTTCATGC AGACATGCAAAACCAACTGTCTCGGTGGTGAGGAGCCATGGGGAGCTCTCCGAAGGGCTT TCCAAGCAGTGGGCTAATGGGCAAAATGACTACTCAATGGCCCTGCTTACCGATGGTACT GATGTGCCAAGGAATATCTATCAGCCCATCCGAAAATATTGAAACAAATGCTTGAGTTTC TCTTCCCTAAAGTAGCAAAGATACCGTAGGCACCACGACTTGACAGCCAGATGGGACCAC TTGAGTTGAGGCGTGTAATGCTGTCCTGTATTAGCACCCCCAAATCTCGTCAAGCCCAAA GGCCTTGCCCATCTGTGATCTTCCACATGTACAGAACCCAGCGTAGTTACGCCAAGTCTT TTGACACGGCCTTCACGAAGTGGCAGCCGACTACNTGTTGCCAATGGTGCCAATTAGGGT AAGAACCGGAAGGCTTAAGGGAAGGCGTTCTCCCACGGCGGAGAT |
Restriction Sites | Please inquire |
ACCN | NM_000746 |
Insert Size | 4900 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000746.2, NP_000737.1 |
RefSeq Size | 2155 bp |
RefSeq ORF | 1509 bp |
Locus ID | 1139 |
UniProt ID | P36544 |
Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
Protein Pathways | Calcium signaling pathway |
Gene Summary | The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (1) lacks an in-frame portion of the 5' coding region, compared to variant 2. The resulting isoform (1) has a shorter N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
NACHO Engages N-Glycosylation ER Chaperone Pathways for α7 Nicotinic Receptor Assembly
,Kweon, HJ;Gu, S;Witham, E;Dhara, M;Yu, H;Mandon, ED;Jawhari, A;Bredt, DS;,
Cell Rep
,PubMed ID 32783947
[CHRNA7]
|
Uniquely human CHRFAM7A gene increases the hematopoietic stem cell reservoir in mice and amplifies their inflammatory response
,Costantini, TW;Chan, TW;Cohen, O;Langness, S;Treadwell, S;Williams, E;Eliceiri, BP;Baird, A;,
Proc. Natl. Acad. Sci. U.S.A.
,PubMed ID 30944217
[CHRNA7]
|
{alpha}7 Nicotinic Acetylcholine Receptor Regulates Airway Epithelium Differentiation by Controlling Basal Cell Proliferation
,Kamel Maouche, Myriam Polette, Thomas Jolly, Kahina Medjber, Isabelle Cloëz-Tayarani, Jean-Pierre Changeux, Henriette Burlet, Christine Terryn, Christelle Coraux, Jean-Marie Zahm, Philippe Birembaut, and Jean-Marie Tournier,
Am. J. Pathol., Nov 2009; 175: 1868 - 1882
[CHRNA7]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221382 | CHRNA7 (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1 |
CNY 5,608.00 |
|
RC221382L1 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1, Myc-DDK-tagged |
CNY 8,008.00 |
|
RC221382L2 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1, mGFP tagged |
CNY 6,270.00 |
|
RC221382L3 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1, Myc-DDK-tagged |
CNY 8,008.00 |
|
RC221382L4 | Lenti ORF clone of Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1, mGFP tagged |
CNY 8,008.00 |
|
RG221382 | CHRNA7 (tGFP-tagged) - Human cholinergic receptor, nicotinic, alpha 7 (CHRNA7), transcript variant 1 |
CNY 7,208.00 |
其它Nicotinic Acetylcholine Receptor alpha 7产品