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OGT (NM_181672) Human Untagged Clone
CAT＃: SC124261
OGT (untagged)-Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1
CNY 10,936.00
| Cited in 2 publications. |
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HINCUT-1; HRNT1; MRX106; O-GLCNAC; OGT1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_181672, the custom clone sequence may differ by one or more nucleotides
ATGGCGTCTTCCGTGGGCAACGTGGCCGACAGCACAGAACCAACGAAACGTATGCTTTCCTTCCAAGGGT TAGCTGAGTTGGCACATCGAGAATATCAGGCAGGAGATTTTGAGGCAGCTGAGAGACACTGCATGCAGCT CTGGAGACAAGAGCCAGACAATACTGGTGTGCTTTTATTACTTTCATCTATACACTTCCAGTGTCGAAGG CTGGACAGATCTGCTCACTTTAGCACTCTGGCAATTAAACAGAACCCCCTTCTGGCAGAAGCTTATTCGA ATTTGGGGAATGTGTACAAGGAAAGAGGGCAGTTGCAGGAGGCAATTGAGCATTATCGACATGCATTGCG TCTCAAACCTGATTTCATCGATGGTTATATTAACCTGGCAGCCGCCTTGGTAGCAGCGGGTGACATGGAA GGGGCAGTACAAGCTTACGTCTCTGCTCTTCAGTACAATCCTGATTTGTACTGTGTTCGCAGTGACCTGG GGAACCTGCTCAAAGCCCTGGGTCGCTTGGAAGAAGCCAAGGCATGTTATTTGAAAGCAATTGAGACGCA ACCGAACTTTGCAGTAGCTTGGAGTAATCTTGGCTGTGTTTTCAATGCACAAGGGGAAATTTGGCTTGCA ATTCATCACTTTGAAAAGGCTGTCACCCTTGACCCAAACTTTCTGGATGCTTATATCAATTTAGGAAATG TCTTGAAAGAGGCACGCATTTTTGACAGAGCTGTGGCAGCTTATCTTCGTGCCCTAAGTTTGAGTCCAAA TCACGCAGTGGTGCACGGCAACCTGGCTTGTGTATACTATGAGCAAGGCCTGATAGATCTGGCAATAGAC ACCTACAGGCGGGCTATCGAACTACAACCACATTTCCCTGATGCTTACTGCAACCTAGCCAATGCTCTCA AAGAGAAGGGCAGTGTTGCTGAAGCAGAAGATTGTTATAATACAGCTCTCCGTCTGTGTCCCACCCATGC AGACTCTCTGAATAACCTAGCCAATATCAAACGAGAACAGGGAAACATTGAAGAGGCAGTTCGCTTGTAT CGTAAAGCATTAGAAGTCTTCCCAGAGTTTGCTGCTGCCCATTCAAATTTAGCAAGTGTACTGCAGCAGC AGGGAAAACTGCAGGAAGCTCTGATGCATTATAAGGAGGCTATTCGAATCAGTCCTACCTTTGCTGATGC CTACTCTAATATGGGAAACACTCTAAAGGAGATGCAGGATGTTCAGGGAGCCTTGCAGTGTTATACGCGT GCCATCCAAATTAATCCTGCATTTGCAGATGCACATAGCAATCTGGCTTCCATTCATAAGGATTCAGGGA ATATTCCAGAAGCCATAGCTTCTTACCGCACGGCTCTGAAACTTAAGCCTGATTTTCCTGATGCTTATTG TAACTTGGCTCATTGCCTGCAGATTGTCTGTGATTGGACAGACTATGATGAGCGAATGAAGAAGTTGGTC AGTATTGTGGCTGACCAGTTAGAGAAGAATAGGTTGCCTTCTGTGCATCCTCATCATAGTATGCTATATC CTCTTTCTCATGGCTTCAGGAAGGCTATTGCTGAGAGGCACGGCAACCTGTGCTTAGATAAGATTAATGT TCTTCATAAACCACCATATGAACATCCAAAAGACTTGAAGCTCAGTGATGGTCGGCTGCGTGTAGGATAT GTGAGTTCCGACTTTGGGAATCATCCTACTTCTCACCTTATGCAGTCTATTCCAGGCATGCACAATCCTG ATAAATTTGAGGTGTTCTGTTATGCCCTGAGCCCAGACGATGGCACAAACTTCCGAGTGAAGGTGATGGC AGAAGCCAATCATTTCATTGATCTTTCTCAGATTCCATGCAATGGAAAAGCAGCTGATCGCATCCATCAG GATGGAATTCATATCCTTGTAAATATGAATGGCTATACTAAGGGCGCTCGAAATGAGCTTTTTGCTCTCA GGCCAGCTCCTATTCAGGCAATGTGGCTGGGATACCCTGGGACGAGTGGTGCGCTTTTCATGGATTATAT TATCACTGATCAGGAAACTTCGCCAGCTGAAGTTGCTGAGCAGTATTCCGAGAAATTGGCTTATATGCCC CACACTTTTTTTATTGGTGATCATGCTAATATGTTCCCTCACCTGAAGAAAAAAGCAGTCATCGATTTTA AGTCCAATGGGCACATTTATGACAATCGGATAGTTCTGAATGGCATCGACCTCAAAGCATTTCTTGATAG TCTACCAGATGTGAAAATTGTCAAGATGAAGTGTCCTGATGGAGGAGACAATGCAGATAGCAGTAACACA GCTCTTAATATGCCTGTTATTCCTATGAATACTATTGCAGAAGCAGTTATTGAAATGATTAACCGAGGAC AGATTCAAATAACAATTAATGGATTCAGTATTAGCAATGGACTGGCAACTACTCAGATCAACAATAAGGC TGCAACTGGAGAGGAGGTTCCCCGTACCATTATTGTAACCACCCGTTCTCAGTACGGGTTACCAGAAGAT GCCATCGTATACTGTAACTTTAATCAGTTGTATAAAATTGACCCTTCTACTTTGCAGATGTGGGCAAACA TTCTGAAGCGTGTTCCCAATAGTGTACTCTGGCTGTTGCGTTTTCCAGCAGTAGGAGAACCTAATATTCA ACAGTATGCACAAAACATGGGCCTGCCCCAGAACCGTATCATTTTTTCACCTGTTGCTCCTAAAGAGGAA CACGTCAGGAGAGGCCAGCTGGCTGATGTCTGCTTGGACACTCCACTCTGTAATGGGCACACCACAGGGA TGGATGTCCTCTGGGCAGGGACCCCCATGGTGACTATGCCAGGAGAGACTCTTGCTTCTCGAGTTGCAGC ATCCCAGCTCACTTGCTTAGGTTGTCTTGAGCTTATTGCTAAAAACAGACAAGAATATGAAGACATAGCT GTGAAGCTGGGAACTGATCTAGAATACCTGAAGAAAGTTCGTGGCAAAGTCTGGAAGCAAAGAATATCTA GCCCTCTGTTCAACACCAAACAATACACAATGGAACTAGAGCGGCTCTATCTACAGATGTGGGAGCATTA TGCAGCTGGCAACAAACCTGACCACATGATTAAGCCTGTTGAAGTCACTGAGTCAGCATAA >OriGene 5' read for NM_181672 unedited
ATAGGCCGGCCGCGAATTCGCACGAGGGTCGCCGCCATTTCAATACCGTACTAGGTAGAT GGTCAATTAGAGTTCCCAGGGTTTGAAGCCTGTAACTGCTGCCGCCGCTCAAGCCCTCCA GAGCATTGCTACGGCTGCTGCCCTTGTACTACTACCTCCAAATACGTTCTTGCTGGTAGT GGCGGCAGCAGGACCAATTACCTCTTTTTTGCTCTCCCTCGAGAAGCTCCAGATGGCGTC TTCCGTGGGCAACGTGGCCGACAGCACAGAACCAACGAAACGTATGCTTTCCTTCCAAGG GTTAGCTGAGTTGGCACATCGAGAATATCAGGCAGGAGATTTTGAGGCAGCTGAGAGACA CTGCATGCAGCTCTGGAGACAAGAGCCAGACAATACTGGTGTGCTTTTATTACTTTCATC TATACACTTCCAGTGTCGAAGGCTGGACAGATCTGCTCACTTTAGCACTCTGGCAATTAA ACAGAACCCCCTTCTGGCAGAAGCTTATTCGAATTTGGGGAATGTGTACAAGGAAAGAGG GCAGTTGCAGGAGGCAATTGAGCATTATCGACATGCATTGCGTCTCAAACCTGATTTCAT CGATGGTTATATTAACCTGNCAGCCGCCTTGGTAGCAGCGGGTGACATGGAANGNGCAGT ACAAGCTTACGTCTCTGCTCTTCAGTACAATCCTGATTTGTACTGTGTNCGCAGTGACCT GNGGAACCTGCTCAAAGCCTGNGTCGCTTGGAAGAGCCAGNGTAGTGGTTGATAGACACA TTTAACATCAGTATATGAAAAACTGTACTTTTTGCCAAGTCTTCAACTCTTCATTGAGCT ATCTTCACAAACAGTCCTTTGAACTNNGAGAAACTGACGGCACGATTCGCCTAAAATAAA CAGGGCCAGGCCTTGGCATATTTGTTCTAAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_181672 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181672.1, NP_858058.1 |
| RefSeq Size | 5475 bp |
| RefSeq ORF | 3141 bp |
| Locus ID | 8473 |
| UniProt ID | O15294 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, O-Glycan biosynthesis |
| Gene Summary | This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Triptolide-induced cell death in pancreatic cancer is mediated by O-GlcNAc modification of transcription factor Sp1
,Banerjee, S;Sangwan, V;McGinn, O;Chugh, R;Dudeja, V;Vickers, SM;Saluja, A;,
J Biol Chem. 2013 Nov 22;288(47):33927-33938
,PubMed ID 24129563
[OGT]
|
|
Modification of RelA by O-linked N-acetylglucosamine links glucose metabolism to NF-B acetylation and transcription
,David F. Allison, J. Jacob Wamsley, Manish Kumar, Duo Li, Lisa G. Gray, Gerald W. Hart, David R. Jones, and Marty W. Mayo,
PNAS, Oct 2012; 109: 16888 - 16893.
[OGT]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206822 | OGT (Myc-DDK-tagged)-Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1 |
CNY 10,928.00 |
|
| RC206822L1 | Lenti ORF clone of Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1, Myc-DDK-tagged |
CNY 13,328.00 |
|
| RC206822L2 | Lenti ORF clone of Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1, mGFP tagged |
CNY 13,328.00 |
|
| RC206822L3 | Lenti ORF clone of Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1, Myc-DDK-tagged |
CNY 10,260.00 |
|
| RC206822L4 | Lenti ORF clone of Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1, mGFP tagged |
CNY 13,328.00 |
|
| RG206822 | OGT (tGFP-tagged) - Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1 |
CNY 12,528.00 |
