DDX5 (NM_004396) Human Untagged Clone

CAT#: SC125285

DDX5 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5)



  "NM_004396" in other vectors (6)

CNY 4,576.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDX5 Rabbit monoclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms G17P1; HLR1; HUMP68; p68
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC125285 sequence for NM_004396 edited (data generated by NextGen Sequencing)
ATGTCGGGTTATTCGAGTGACCGAGACCGCGGCCGGGACCGAGGGTTTGGTGCACCTCGA
TTTGGAGGAAGTAGGGCAGGGCCCTTATCTGGAAAGAAGTTTGGAAACCCTGGGGAGAAA
TTAGTTAAAAAGAAGTGGAATCTTGATGAGCTGCCTAAATTTGAGAAGAATTTTTATCAA
GAGCACCCTGATTTGGCTAGGCGCACAGCACAAGAGGTGGAAACATACAGAAGAAGCAAG
GAAATTACAGTTAGAGGTCACAACTGCCCGAAGCCAGTTCTAAATTTTTATGAAGCCAAT
TTCCCTGCAAATGTCATGGATGTTATTGCAAGACAGAATTTCACTGAACCCACTGCTATT
CAAGCTCAGGGATGGCCAGTTGCTCTAAGTGGATTGGATATGGTTGGAGTGGCACAGACT
GGATCTGGGAAAACATTGTCTTATTTGCTTCCTGCCATTGTCCACATCAATCATCAGCCA
TTCCTAGAGAGAGGCGATGGGCCTATTTGTTTGGTGCTGGCACCAACTCGGGAACTGGCC
CAACAGGTGCAGCAAGTAGCTGCTGAATATTGTAGAGCATGTCGCTTGAAGTCTACTTGT
ATCTACGGTGGTGCTCCTAAGGGACCACAAATACGTGATTTGGAGAGAGGTGTGGAAATC
TGTATTGCAACACCTGGAAGACTGATTGACTTTTTAGAGTGTGGAAAAACCAATCTGAGA
AGAACAACCTACCTTGTCCTTGATGAAGCAGATAGAATGCTTGATATGGGCTTTGAACCC
CAAATAAGGAAGATTGTGGATCAAATAAGACCTGATAGGCAAACTCTAATGTGGAGTGCG
ACTTGGCCAAAAGAAGTAAGACAGCTTGCTGAAGATTTCCTGAAAGACTATATTCATATA
AACATTGGTGCACTTGAACTGAGTGCAAACCACAACATTCTTCAGATTGTGGATGTGTGT
CATGACGTAGAAAAGGATGAAAAACTTATTCGTCTAATGGAAGAGATCATGAGTGAGAAG
GAGAATAAAACCATTGTTTTTGTGGAAACCAAAAGAAGATGTGATGAGCTTACCAGAAAA
ATGAGGAGAGATGGGTGGCCTGCCATGGGTATCCATGGTGACAAGAGTCAACAAGAGCGT
GACTGGGTTCTAAATGAATTCAAACATGGAAAAGCTCCTATTCTGATTGCTACAGATGTG
GCCTCCAGAGGGCTAGATGTGGAAGATGTGAAATTTGTCATCAATTATGACTACCCTAAC
TCCTCAGAGGATTATATTCATCGAATTGGAAGAACTGCTCGCAGTACCAAAACAGGCACA
GCATACACTTTCTTTACACCTAATAACATAAAGCAAGTGAGCGACCTTATCTCTGTGCTT
CGTGAAGCTAATCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAGGTTCA
GGTCGTTCCAGGGGTAGAGGAGGCATGAAGGATGACCGTCGGGACAGATACTCTGCGGGC
AAAAGGGGTGGATTTAATACCTTTAGAGACAGGGAAAATTATGACAGAGGTTACTCTAGC
CTGCTTAAAAGAGATTTTGGGGCAAAAACTCAGAATGGTGTTTACAGTGCTGCAAATTAC
ACCAATGGGAGCTTTGGAAGTAATTTTGTGTCTGCTGGTATACAGACCAGTTTTAGGACT
GGTAATCCAACAGGGACTTACCAGAATGGTTATGATAGCACTCAGCAATACGGAAGTAAT
GTTCCAAATATGCACAATGGTATGAACCAACAGGCATATGCATATCCTGCTACTGCAGCT
GCACCTATGATTGGTTATCCAATGCCAACAGGATATTCCCAATAA

Clone variation with respect to NM_004396.3
>OriGene 5' read for NM_004396 unedited
GGGGGCGGGGGCNCCCNNNNNNTTTTNCNNNNNCGGTTCAGATTTGTATACAACTCATAT
AGGCGGCCGCGNAATTCGCACGAGCCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATC
GGTGTCCTTCCTCCGCTGCCGCCCCCGCAAGGCTTCGCCGTCATCGAGGCCATTTCCAGC
GACTTGTCGCACGCTTTTCTATATACTTCGTTCCCCGCCAACCGCAACCATTGACGCCAT
GTCGGGTTATTCGAGTGACCGAGACCGCGGCCGGGACCGAGGGTTTGGTGCACCTCGATT
TGGAGGAAGTAGGGCAGGGCCCTTATCTGGAAAGAAGTTTGGAAACCCTGGGGAGAAATT
AGTTAAAAAGAAGTGGAATCTTGATGAGCTGCCTAAATTTGAGAAGAATTTTTATCAAGA
GCACCCTGATTTGGCTAGGCGCACAGCACAAGAGGTGGAAACATACAGAAGAAGCAAGGA
AATTACAGTTAGAGGTCACAACTGCCCGAAGCCAGTTCTAAATTTTTATGAAGCCAATTT
CCCTGCAAATGTCATGGATGTTATTGCAAGACAGAATTTCACTGAACCCACTGCTATTCA
AGCTCAGGGATGGCCAGTTGCTCTAAGTGGATTGGATATGGTTGGAGTGGCACAGACTGG
ATCTGGGAAAACATTGTCTTATTTGCTTCCTGCCATTGTCCACATCAATCATCAGCCATT
CCTAGAGAGAGGCGATGGGCCTATTTGTTTGGTGCTGGCACCAACTCGGGAACTGGCCCA
ACAGGTGCAGCAAGTAGCTGCTGAATATTGTAGAGCATGTCGCTTGAAGTCTACTTGTAT
CTACNGTGGTGCTCCTAAGGGACCCAAATACNTGATTGGGAGAGAGGTGTGAAATCTGTA
TTGCACACCTGAAGAAACTGATGACTTTTANATNGTGAANAACCATCTGAGAG
>OriGene 3' read for NM_004396 unedited
NNNNCCCCCTCCTGGGCCATGGTGATGGCACTTGCCAGGNNCCAGNGANGAGCACTGGGG
NAGGGAGTCACAGGGCATGCCACCCGGGTACTGTTCAGGAAACAGCTATGACCGCGGCCG
CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCAGTCTTCAAATATTTTTTATTGGAA
GGCCATGCATTGCCTTCATTTATTGTATTTCAAATCACTGTACATTTACTTTTGTGAAAA
CACTGCCTGCATTTTCTAGTACAAAAAACACCTAAAAATTGTTTCAGGAATGTAGAGAAA
TATCCAACTTAAATAGCGAAAAAGTGCACCATAATTACTGCTGCACTGCAGTCATTTCTG
CAATTCCATGTTTCTTAAAAACTATCTTGTCAGATAACACACATATTAAGAGCAATTATG
AAAAACAGACTTTACATATACTTCTAAAGTCTTATTGGGAATATCCTGTTGGCATTGGAA
TACCAATCATAGGTGCAGCTGCAGTAACAGGATATGCATATGCCCTGTTGGTTCATACCA
TTGGGCTATTTTGAACATTACTTCCGTATGGCTGAGTGCTATCATAACCATTCTGTAAGT
CCCTGTTGGATACCATTCCAAAACTGGGCTGGTTCCAACCAGACCCAAATTACTTTCAAA
GCCTTCCATGGGTGAATTTGGAGCACTGAAAAACCCTTTTTGATTTTTTGCCCAAAATCT
TTTTAAGCGAGCTAAAATAACCTCTGGCAAAATTTTCCCCGGGCTTAAAGGGTTTAAATC
CCCCCTTTTGGCCCCAAAAATTTTTTTCCCCAAGGTAATCCTTAGGCCCCCCTTACCCGG
GAACGACCTGAACCTTGTTTTTCACCAAATGAAACACTTGGAAATAATGCCTTGATACCC
TCCCA
Restriction Sites NotI-NotI     
ACCN NM_004396
Insert Size 2550 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004396.2, NP_004387.1
RefSeq Size 2325 bp
RefSeq ORF 1845 bp
Locus ID 1655
UniProt ID P17844
Domains DEAD, helicase_C
Protein Pathways Spliceosome
Gene Summary This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 through 3 encode the same isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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