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AMPK beta 1 (PRKAB1) (NM_006253) Human Untagged Clone
CAT＃: SC125443
PRKAB1 (untagged)-Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)
CNY 6,270.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AMPK; HAMPKb |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006253, the custom clone sequence may differ by one or more nucleotides
ATGGGCAATACCAGCAGTGAGCGCGCCGCGCTGGAGCGGCATGGTGGCCATAAGACGCCCCGGAGGGACA GCTCGGGGGGCACCAAGGACGGGGACAGGCCCAAGATCCTGATGGACAGCCCCGAAGACGCCGACCTCTT CCACTCCGAGGAAATCAAGGCACCAGAGAAGGAGGAATTCCTGGCCTGGCAGCATGATCTGGAAGTGAAT GATAAAGCTCCCGCCCAGGCTCGGCCAACGGTGTTTCGATGGACGGGGGGCGGAAAGGAAGTTTACTTAT CTGGGTCCTTCAACAACTGGAGTAAACTTCCCCTCACCAGAAGCCACAATAACTTTGTAGCCATCCTGGA TCTGCCGGAAGGAGAGCATCAGTACAAGTTCTTTGTGGATGGTCAGTGGACGCACGACCCTTCCGAGCCC ATAGTAACCAGCCAGCTTGGCACAGTTAACAACATCATTCAAGTGAAGAAAACTGACTTTGAAGTATTTG ATGCTTTAATGGTGGATTCCCAAAAGTGCTCCGATGTGTCTGAGCTGTCCAGTTCTCCCCCAGGACCCTA CCATCAGGAGCCCTACGTCTGCAAACCCGAAGAGCGCTTTCGGGCACCCCCTATTCTCCCCCCACATCTC CTCCAGGTCATCCTGAACAAGGACACGGGGATTTCCTGTGATCCAGCTTTGCTTCCTGAGCCCAATCACG TCATGCTGAACCACCTATACGCGCTGTCTATCAAGGATGGAGTGATGGTGCTCAGCGCAACCCACCGGTA CAAGAAGAAGTACGTCACCACCTTGTTATACAAGCCCATATGA >OriGene 5' read for NM_006253 unedited
NNGGTCAACATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCAGGTA AAGCGCGATTGCGAGAGCTCGGCAACCCTGCCGACTCAGCCGGAACCGGCTCCCGGCCCG AGGGGCGTGGTGTCCTGGTGCTCCGACTCCTTCCGCAGGCTCCTTGGGACCCGCGGTTCC GGGAGTCCCTTGCTCAGGGTCCCTTTCCTGCAGTGAGGCGCCGTCCGCCTTCCCTGTGTC CCCGCAGACCCCCATCATGGGCAATACCAGCAGTGAGCGCGCCGCGCTGGAGCGGCATGG TGGCCATAAGACGCCCCGGAGGGACAGCTCGGGGGGCACCAAGGACGGGGACAGGCCCAA GATCCTGATGGACAGCCCCGAAGACGCCGACCTCTTCCACTCCGAGGAAATCAAGGCACC AGAGAAGGAGGAATTCCTGGCCTGGCAGCATGATCTGGAAGTGAATGATAAAGCTCCCGC CCAGGCTCGGCCAACGGTGTTTCGATGGACGGGGGGCGGAAAGGAAGTTTACTTATCTGG GTCCTTCAACAACTGGAGTAAACTTCCCCTCACCAGAAGCCACAATAACTTTGTAGCCAT CCTGGATCTGCCGGAAGGAGAGCATCAGTACAAGTTCTTTGTGGATGGTCAGTGGACGCA CGACCCTTCCGAGCCCATAGTAACCAGCCAGCTTGGCACAGTTAACAACATCATTCAAGT GAAGAAAACTGACTNTGAAGTATTTGATGCTTTAATGGTGGATTCCCAAAAGTGCTCCGA TGTGTCTGAGCTGTCCAGTTCTCCCCCAGACCCTACCATCANGAGCCCTACGTCTGCAAC CCCGAAGAGCGCTTTCGGGCACCCNCTATTCTCCCCCACATCTNCTNCAGNTCATTNCTG ACAAGGACACNGNGATTTCCTGTGATCCAGCTTTGCTTCCTGAGCCCC >OriGene 3' read for NM_006253 unedited
NGACCTTTGGGATGGCAATCTCCATTTCCAGNANAGCACTGGGGCAGGGTCACAGGGATG CCACCCGGGATCTGTTCAGGAAAAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTT TTTTTTTTTTTTTTACAAAGTTTTATTGAAACTTGTCCATCAAGGTGACAGTAATTCACT GTTTTGAAAAGTCACAAAAAAGGGTACCCCCAAACTCAGGTATACCAAGTAAATACTGGA ACCCAGGGAGCTCACTCCTCCCTCTCCCACCAGGAGCAAAGGGCATAACGGGAGCTTCCG CTGACTCACAGCAACCTGGGCTAGTGGGTACATCTGTGGCGACTCCCGGGAAACTGAGCT GCGTATAAAGAAGTCTGCTTTGGTCCAAAGGACATCTTCCTGACCCAGACTCAGAAGGCT GCTGTCAAGTTCTACATTCCTTTCAGAATAAGCAACAGCAACTGCTGCAGTCTTGCTAAC TAGGGGCAGAGGTCCTGAGCAAGGTGCCACGGGACCTTGTAAAATCACGCTGTGTGATTT TATAGGGAAGAGGGCAGAGTGCCTCTCAAGTTTTCACGGTTAACACTGTCACCTATTATG ACTGGAATAAAAAGCTGGACAACCTGCCAGCTAACTGGTCAGTCCTGAGGATCATCCAGC TGAATGGAGAAATCCGGCAGCAGGTTGAAAAGTCTGTTCTGAGAACAGATTATTGCTGAG CAGAGTTCATGTGACTTCTAGACGTGGTGACTTAAAAAATGGCCTTAANGCTGCAGAGCC AGCCACCTCTGCTTACAAAAAGAGTACTTAGTGCACATGACTGTNAGAAACATTTGTAAA ACCTCATCTAGAAATCAGAAAGCTTNCTATTTCTATANAATGACACCTNCCTGGNACCGA GAGACATTCTTGTGTTAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006253 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006253.4, NP_006244.2 |
| RefSeq Size | 2412 bp |
| RefSeq ORF | 813 bp |
| Locus ID | 5564 |
| UniProt ID | Q9Y478 |
| Domains | isoamylase_N, AMPKBI |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway |
| Gene Summary | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The energy sensor AMP-activated protein kinase directly regulates the mammalian FOXO3 transcription factor
,Eric L Greer, Philip R Oskoui, Max R Banko, Jay M Maniar, Melanie P Gygi, Steven P Gygi, and Anne Brunet,
J Biol Chem. 2007 Oct 12;282(41):30107-19.
[PRKAB1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203911 | PRKAB1 (Myc-DDK-tagged)-Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1) |
CNY 2,400.00 |
|
| RC203911L1 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203911L2 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1), mGFP tagged |
CNY 4,800.00 |
|
| RC203911L3 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203911L4 | Lenti ORF clone of Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1), mGFP tagged |
CNY 4,800.00 |
|
| RG203911 | PRKAB1 (tGFP-tagged) - Human protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1) |
CNY 4,000.00 |
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