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KDM5B (NM_006618) Human Untagged Clone
CAT＃: SC125457
KDM5B (untagged)-Human lysine (K)-specific demethylase 5B (KDM5B)
CNY 10,568.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CT31; JARID1B; MRT65; PLU-1; PLU1; PPP1R98; PUT1; RBBP2H1A; RBP2-H1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_006618 edited
ATGGAGGCGGCCACCACACTGCACCCAGGCCCGCGCCCGGCGCTGCCCCTCGGGGGCCCG GGCCCGCTGGGCGAGTTCCTGCCTCCACCCGAGTGCCCGGTCTTCGAACCCAGCTGGGAA GAGTTCGCGGACCCCTTCGCTTTCATCCACAAGATCCGGCCCATAGCCGAGCAGACTGGC ATCTGTAAGGTGCGGCCGCCGCCGGATTGGCAGCCACCATTTGCATGTGATGTTGATAAA CTTCATTTTACGCCACGTATCCAGAGACTGAATGAATTGGAGGCCCAAACTCGTGTAAAA TTGAATTTCTTGGACCAGATTGCAAAGTACTGGGAGTTACAGGGAAGTACTCTGAAAATT CCACATGTGGAGAGGAAGATCTTGGACTTATTTCAGCTTAATAAGTTAGTTGCAGAAGAA GGTGGATTTGCAGTTGTTTGCAAGGATAGAAAATGGACCAAAATTGCTACCAAGATGGGG TTTGCTCCTGGCAAAGCAGTGGGCTCACATATCAGAGGGCATTATGAACGAATTCTCAAC CCCTACAACTTATTCCTGTCCGGAGACAGCCTAAGGTGTTTGCAGAAGCCAAACCTGACC ACAGACACTAAGGACAAGGAGTACAAACCCCATGATATTCCCCAGAGGCAGTCTGTGCAG CCTTCGGAAACGTGCCCCCCAGCCCGACGAGCAAAACGCATGAGAGCAGAGGCCATGAAT ATTAAAATAGAACCCGAGGAGACAACAGAAGCCAGAACTCATAATCTGAGACGTCGAATG GGTTGTCCAACTCCAAAATGTGAAAATGAGAAAGAAATGAAGAGTAGCATCAAGCAAGAA CCTATTGAGAGGAAAGATTATATTGTAGAAAATGAGAAGGAAAAGCCCAAGAGTCGATCT AAAAAAGCCACCAATGCTGTGGACCTGTATGTCTGTCTTTTATGTGGCAGTGGCAATGAT GAAGACCGGCTACTGTTGTGTGATGGCTGTGATGACAGTTACCATACCTTTTGCTTGATC CCACCTCTCCATGATGTTCCCAAGGGAGACTGGAGGTGTCCTAAGTGTTTGGCTCAGGAA TGTAGTAAGCCACAAGAAGCATTTGGCTTTGAACAAGCAGCCAGGGACTATACCCTCCGT ACTTTTGGGGAAATGGCAGATGCGTTCAAATCTGATTACTTCAACATGCCAGTCCATATG GTCCCCACAGAGCTTGTTGAGAAAGAATTTTGGAGACTAGTAAGCACTATTGAGGAGGAT GTCACAGTGGAATATGGAGCTGACATTGCCTCAAAGGAATTTGGCAGTGGCTTTCCTGTC CGAGATGGGAAAATCAAACTCTCACCTGAGGAAGAGGAGTATCTTGATAGTGGCTGGAAT TTGAACAACATGCCAGTGATGGAGCAGTCTGTCCTTGCACATATTACTGCTGATATATGT GGCATGAAACTTCCTTGGTTGTATGTGGGAATGTGCTTTTCTTCATTCTGTTGGCACATT GAAGACCACTGGAGCTATTCAATTAACTACTTGCACTGGGGTGAGCCAAAAACCTGGTAT GGAGTCCCAGGGTATGCTGCTGAGCAGCTAGAAAATGTAATGAAGAAACTAGCTCCAGAA CTCTTTGTGTCCCAGCCGGATCTCCTCCATCAGCTTGTGACCATCATGAACCCCAATACC CTGATGACTCATGAAGTGCCTGTTTACCGAACTAATCAGTGTGCTGGGGAGTTTGTGATT ACATTTCCAAGAGCCTACCACAGTGGTTTTAACCAGGGTTTTAATTTTGCTGAGGCTGTT AACTTCTGCACTGTTGATTGGCTGCCATTAGGCCGACAGTGTGTGGAGCATTATCGCTTG CTTCATCGATATTGTGTGTTTTCCCATGATGAGATGATCTGCAAGATGGCTTCCAAGGCT GATGTATTAGATGTTGTAGTGGCTTCAACTGTTCAGAAAGACATGGCCATTATGATTGAG GATGAGAAAGCTTTAAGAGAAACTGTCCGTAAATTGGGAGTGATTGATTCGGAAAGAATG GATTTTGAGCTGTTGCCAGATGATGAACGTCAGTGTGTAAAATGCAAAACTACATGCTTC ATGTCTGCCATCTCCTGTTCTTGTAAACCTGGCCTTCTTGTTTGCCTGCATCATGTAAAA GAATTGTGTTCCTGTCCTCCTTATAAATATAAATTGCGGTATAGGTACACGCTGGATGAT CTCTACCCTATGATGAATGCATTGAAGCTTCGAGCAGAATCTTACAACGAATGGGCCTTG AATGTGAATGAAGCTTTGGAGGCAAAGATCAACAAGAAGAAAAGCCTTGTCAGCTTCAAG GCTTTAATTGAAGAATCTGAAATGAAGAAATTCCCAGACAATGATCTTTTGCGACACCTT CGCCTAGTCACACAGGATGCAGAGAAGTGTGCCTCTGTTGCGCAGCAGTTGCTTAATGGC AAAAGGCAAACTAGATATCGATCTGGTGGAGGGAAATCCCAAAATCAGTTGACAGTGAAT GAGCTCCGGCAGTTTGTAACACAGCTGTATGCTCTTCCATGTGTCCTCAGTCAGACACCA TTACTAAAGGATCTCTTGAATCGTGTAGAAGATTTTCAACAGCATAGTCAGAAACTACTC TCTGAGGAAACGCCTAGTGCTGCGGAGCTGCAGGACTTGCTAGATGTCAGCTTTGAATTT GATGTTGAACTTCCACAGCTTGCTGAGATGCGTATCCGTTTGGAACAAGCCCGTTGGCTA GAAGAGGTGCAGCAAGCTTGCCTAGACCCCAGCTCCCTTACTTTAGATGATATGAGACGT CTCATAGACCTAGGGGTAGGGCTGGCCCCGTATTCAGCAGTGGAGAAAGCTATGGCCCGG CTGCAGGAACTGCTCACAGTGTCAGAGCACTGGGACGACAAAGCCAAGAGTCTCCTCAAG GCCAGGCCACGACATTCATTGAATAGCCTTGCTACGGCAGTAAAGGAAATCGAAGAGATC CCTGCATATCTGCCCAATGGTGCGGCTCTGAAAGACTCAGTGCAGAGAGCCAGAGACTGG CTTCAGGATGTAGAGGGCCTGCAGGCTGGAGGACGTGTGCCAGTGTTAGACACACTCATA GAACTTGTTACACGAGGCCGATCTATCCCGGTACATCTGAATTCTTTGCCAAGACTGGAA ACCCTAGTAGCTGAGGTTCAGGCTTGGAAAGAATGTGCTGTTAATACATTCTTGACTGAG AATTCTCCATATTCTCTCTTAGAGGTGCTGTGTCCTCGATGTGATATTGGCCTTTTGGGA TTGAAAAGGAAGCAGAGAAAGTTAAAGGAGCCCTTGCCAAATGGAAAGAAAAAAAGCACC AAATTAGAGAGTCTGAGTGACCTGGAGAGAGCTTTAACTGAAAGCAAGGAGACTGCTTCA GCTATGGCAACTCTTGGGGAAGCTCGCCTAAGGGAAATGGAAGCCTTGCAGTCTCTCAGA CTCGCCAATGAAGGGAAATTGCTGTCGCCTCTCCAAGATGTGGATATAAAAATCTGCCTA TGTCAGAAGGCCCCAGCTGCCCCTATGATTCAATGTGAACTCTGCAGGGATGCTTTCCAC ACCAGTTGTGTGGCGGTACCCAGTATTTCACAGGGCCTGCGAATCTGGCTTTGTCCCCAT TGTCGGAGGTCAGAGAAACCTCCATTAGAGAAAATTCTGCCCCTGCTCGCCTCCCTTCAG CGTATCCGAGTTCGCCTTCCTGAGGGAGATGCACTTCGATATATGATTGAAAGAACCGTG AACTGGCAGCACAGAGCCCAGCAACTGCTTTCGTCAGGGAATCTTAAATTTGTGCAAGAT CGAGTGGGCTCAGGACTGTTATATAGCAGATGGCAAGCCTCAGCAGGACAGGTGTCAGAC ACAAACAAGGTATCTCAACCTCCTGGCACAACATCATTTTCTTTGCCTGATGACTGGGAC AACAGAACCTCATATTTGCACTCCCCCTTCTCAACTGGACGAAGTTGTATCCCCCTCCAT GGTGTTAGTCCAGAAGTGAATGAACTATTGATGGAAGCCCAGCTGCTCCAGGTATCCCTT CCTGAAATTCAGGAACTTTACCAGACTTTACTTGCAAAGCCAAGCCCTGCTCAGCAGACT GACCGAAGCTCACCAGTGAGACCCAGCAGTGAGAAGAATGACTGTTGCCGAGGGAAGCGA GATGGAATTAACAGTCTTGAGAGAAAACTGAAGAGACGCCTGGAAAGAGAGGGCCTCTCC AGTGAGCGGTGGGAACGAGTTAAGAAAATGCGGACCCCCAAAAAGAAGAAAATCAAACTG AGCCACCCCAAGGACATGAACAATTTCAAGTTAGAGAGAGAGCGTAGCTATGAATTAGTT CGTTCTGCTGAAACTCATTCCCTGCCCTCAGACACATCCTATTCCGAACAGGAAGACTCT GAGGATGAAGATGCCATCTGCCCAGCTGTGAGCTGCCTGCAGCCAGAAGGAGATGAGGTG GACTGGGTCCAGTGTGATGGCAGCTGCAATCAGTGGTTTCATCAGGTCTGTGTTGGTGTC TCCCCAGAGATGGCAGAGAAAGAAGACTACATCTGTGTGCGCTGTACTGTGAAGGACGCA CCAAGCCGAAAGTAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006618 |
| Insert Size | 6400 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006618.3, NP_006609.3 |
| RefSeq Size | 6393 bp |
| RefSeq ORF | 4635 bp |
| Locus ID | 10765 |
| UniProt ID | Q9UGL1 |
| Domains | ARID, PHD, JmjC, JmjN, zf-C5HC2 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016] Transcript Variant: This variant (2) lacks an exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214918 | KDM5B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 5B (KDM5B) |
CNY 10,568.00 |
|
| RC214918L1 | Lenti ORF clone of Human lysine (K)-specific demethylase 5B (KDM5B), Myc-DDK-tagged |
CNY 12,968.00 |
|
| RC214918L2 | Lenti ORF clone of Human lysine (K)-specific demethylase 5B (KDM5B), mGFP tagged |
CNY 15,680.00 |
|
| RC214918L3 | Lenti ORF clone of Human lysine (K)-specific demethylase 5B (KDM5B), Myc-DDK-tagged |
CNY 15,680.00 |
|
| RC214918L4 | Lenti ORF clone of Human lysine (K)-specific demethylase 5B (KDM5B), mGFP tagged |
CNY 12,968.00 |
|
| RG214918 | KDM5B (tGFP-tagged) - Human lysine (K)-specific demethylase 5B (KDM5B) |
CNY 12,168.00 |
