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FLI1 (NM_002017) Human Untagged Clone
CAT＃: SC125534
FLI1 (untagged)-Human Friend leukemia virus integration 1 (FLI1), transcript variant 1
CNY 5,488.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BDPLT21; EWSR2; SIC-1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC125534 sequence for NM_002017 edited (data generated by NextGen Sequencing)
ATGGACGGGACTATTAAGGAGGCTCTGTCGGTGGTGAGCGACGACCAGTCCCTCTTTGAC TCAGCGTACGGAGCGGCAGCCCATCTCCCCAAGGCCGACATGACTGCCTCGGGGAGTCCT GACTACGGGCAGCCCCACAAGATCAACCCCCTCCCACCACAGCAGGAGTGGATCAATCAG CCAGTGAGGGTCAACGTCAAGCGGGAGTATGACCACATGAATGGATCCAGGGAGTCTCCG GTGGACTGCAGCGTTAGCAAATGCAGCAAGCTGGTGGGCGGAGGCGAGTCCAACCCCATG AACTACAACAGCTATATGGACGAGAAGAATGGCCCCCCTCCTCCCAACATGACCACCAAC GAGAGGAGAGTCATCGTCCCCGCAGACCCCACACTGTGGACACAGGAGCATGTGAGGCAA TGGCTGGAGTGGGCCATAAAGGAGTACAGCTTGATGGAGATCGACACATCCTTTTTCCAG AACATGGATGGCAAGGAACTGTGTAAAATGAACAAGGAGGACTTCCTCCGCGCCACCACC CTCTACAACACGGAAGTGCTGTTGTCACACCTCAGTTACCTCAGGGAAAGTTCACTGCTG GCCTATAATACAACCTCCCACACCGACCAATCCTCACGATTGAGTGTCAAAGAAGACCCT TCTTATGACTCAGTCAGAAGAGGAGCTTGGGGCAATAACATGAATTCTGGCCTCAACAAA AGTCCTCCCCTTGGAGGGGCACAAACGATCAGTAAGAATACAGAGCAACGGCCCCAGCCA GATCCGTATCAGATCCTGGGCCCGACCAGCAGTCGCCTAGCCAACCCTGGAAGCGGGCAG ATCCAGCTGTGGCAATTCCTCCTGGAGCTGCTCTCCGACAGCGCCAACGCCAGCTGTATC ACCTGGGAGGGGACCAACGGGGAGTTCAAAATGACGGACCCCGATGAGGTGGCCAGGCGC TGGGGCGAGCGGAAAAGCAAGCCCAACATGAATTACGACAAGCTGAGCCGGGCCCTCCGT TATTACTATGATAAAAACATTATGACCAAAGTGCACGGCAAAAGATATGCTTACAAATTT GACTTCCACGGCATTGCCCAGGCTCTGCAGCCACATCCGACCGAGTCGTCCATGTACAAG TACCCTTCTGACATCTCCTACATGCCTTCCTACCATGCCCACCAGCAGAAGGTGAACTTT GTCCCTCCCCATCCATCCTCCATGCCTGTCACTTCCTCCAGCTTCTTTGGAGCCGCATCA CAATACTGGACCTCCCCCACGGGGGGAATCTACCCCAACCCCAACGTCCCCCGCCATCCT AACACCCACGTGCCTTCACACTTAGGCAGCTACTACTAG Clone variation with respect to NM_002017.3 >OriGene 5' read for NM_002017 unedited
ACACGATTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAACAACAAACGTGCACAGG GGAGTGAGGGCAGGGCGCTCGCAGGGGGCACGCAGGGAGGGCCCAGGGCGCCAGGGAGGC CGCGCCGGGCTAATCCGAAGGGGCTGCGAGGTCAGGCTGTAACCGGGTCAATGTGTGGAA TATTGGGGGGCTCGGCTGCAGACTTGGCCAAATGGACGGGACTATTAAGGAGGCTCTGTC GGTGGTGAGCGACGACCAGTCCCTCTTTGACTCAGCGTACGGAGCGGCAGCCCATCTCCC CAAGGCCGACATGACTGCCTCGGGGAGTCCTGACTACGGGCAGCCCCACAAGATCAACCC CCTCCCACCACAGCAGGAGTGGATCAATCAGCCAGTGAGGGTCAACGTCAAGCGGGAGTA TGACCACATGAATGGATCCAGGGAGTCTCCGGTGGACTGCAGCGTTAGCAAATGCAGCAA GCTGGTGGGCGGAGGCGAGTCCAACCCCATGAACTACAACAGCTATATGGACGAGAAGAA TGGCCCCCCTCCTCCCAACATGACCACCAACGAGAGGAGAGTCATCGTCCCCGCAGACCC CACACTGTGGACACAGGAGCATGTGAGGCAATGGCTGGAGTGGGCCATAAAGGAGTACAG CTTGATGGAGATCGACACATCCTTTTTCCAGAACATGGATGGCANGGAACTGTGTAAAAT GAACAGGGATGACTTNNCTCGNGCACCACCCTCTACACACGGAAGTGCTGNTGTCACACC TCAGTTACCTCAGGNAATGNTCACTGCTGGNCCTATATACACCTCCACACCGACCATCCT NACGATGAGTGTCAAAGAGACCCTTCTATGACTCATCAGAAGAGAGCCTGGNGGCATACA TGAATTCTGGCCTCACAAAGTCCTCCCCTTGGAGGGNCACAACGATCAGTAGGATACAGA GCC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002017 |
| Insert Size | 2800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002017.2, NP_002008.2 |
| RefSeq Size | 2957 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 2313 |
| UniProt ID | Q01543 |
| Domains | ETS, SAM_PNT |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A novel oncogenic mechanism in Ewing sarcoma involving IGF pathway targeting by EWS/Fli1-regulated microRNAs
,E L McKinsey, J K Parrish, A E Irwin, B F Niemeyer, H B Kern, D K Birks, P Jedlicka,
Oncogene (6 June 2011) doi:10.1038/onc.2011.197 Short Communication
[FLI1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200695 | FLI1 (Myc-DDK-tagged)-Human Friend leukemia virus integration 1 (FLI1), transcript variant 1 |
CNY 5,488.00 |
|
| RC200695L1 | Lenti ORF clone of Human Friend leukemia virus integration 1 (FLI1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
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| RC200695L2 | Lenti ORF clone of Human Friend leukemia virus integration 1 (FLI1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
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| RC200695L3 | Lenti ORF clone of Human Friend leukemia virus integration 1 (FLI1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC200695L4 | Lenti ORF clone of Human Friend leukemia virus integration 1 (FLI1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG200695 | FLI1 (tGFP-tagged) - Human Friend leukemia virus integration 1 (FLI1), transcript variant 1 |
CNY 7,088.00 |
