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TTC35 (EMC2) (NM_014673) Human Untagged Clone
CAT＃: SC126494
EMC2 (untagged)-Human tetratricopeptide repeat domain 35 (TTC35)
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0103; TTC35 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_014673, the custom clone sequence may differ by one or more nucleotides
ATGGCGAAGGTCTCAGAGCTTTACGATGTCACTTGGGAAGAAATGAGAGATAAAATGAGAAAATGGAGAG AAGAAAACTCAAGAAATAGTGAGCAAATTGTGGAAGTTGGAGAAGAATTAATTAATGAATATGCTTCTAA GCTGGGAGATGATATTTGGATCATATATGAACAGGTGATGATTGCAGCACTAGACTATGGTCGGGATGAC TTGGCATTGTTTTGTCTTCAAGAGCTGAGAAGACAGTTCCCTGGCAGTCACAGAGTCAAGCGATTAACAG GCATGAGATTTGAAGCCATGGAAAGATATGATGATGCTATACAGCTATATGATAGGATTTTACAAGAAGA TCCAACTAACACTGCTGCAAGAAAGCGTAAGATTGCCATTCGAAAAGCCCAGGGGAAAAATGTGGAGGCC ATTCGGGAGCTGAATGAGTATCTGGAACAATTTGTTGGAGACCAAGAAGCCTGGCATGAACTTGCAGAAC TTTACATCAATGAACATGACTATGCAAAAGCAGCCTTTTGTTTAGAGGAACTAATGATGACTAATCCACA CAACCACTTATACTGTCAGCAGTATGCTGAAGTTAAGTATACCCAAGGTGGACTTGAAAACCTCGAACTT TCAAGAAAGTATTTTGCACAGGCATTGAAACTGAACAACAGAAATATGAGAGCTTTGTTTGGACTTTATA TGTCGGCAAGTCATATTGCTTCTAATCCAAAAGCAAGTGCAAAAACGAAAAAGGACAACATGAAATATGC TAGTTGGGCAGCTAGTCAAATAAACAGAGCTTATCAGTTTGCAGGTCGAAGTAAGAAGGAAACCAAATAT TCTCTTAAGGCTGTCGAAGACATGTTGGAAACATTGCAGATCACCCAGTCTTAA >OriGene 5' read for NM_014673 unedited
CCCATTTCCCCCGCCCGTTGCCGCATTGGGCGGCAGGCGTGTCCGGTGGGAGGTCTATAT AAGCAGATTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC CGCGAATTCGGCACGAGGGGAAGATGGCGAAGGTCTCAGAGCTTTACGATGTCACTTGGG AAGAAATGAGAGATAAAATGAGAAAATGGAGAGAAGAAAACTCAAGACATAGTGAGCAAA TTGTGGAAGTTGGAGAAGAATTAATTAATGAATATGCTTCTAAGCTGGGAGATGATATTT GGATCATATATGAACAGGTGATGATTGCAGCACTAGACTATGGTCGGGATGACTTGGCAT TGTTTTGTCTTCAAGAGCTGAGAAGACAGTTCCCTGGCAGTCACAGAGTCAAGCGATTAA CAGGCATGAGATTTGAAGCCATGCAAAGATATGATGATGCTATACAGCTATATGATAGGA TTTTACAAGACGATCCAACTAACACTGCTGCAAGAAAGCGTAAGAGTGCCATTCGAAAAG CCCAGGGGAAAAATGTGGAGGCCATTCGGGAGCTGAATGAGTATCTGGAACAATTTGTTG GAGACCAAGACAGCCTGGCATGAACTTGCAGAACTTTACATCAATGAACATGACTATGCA AAAGCAGTCTTTTGTTTAGAGGAACTAATGATGACTAATCCACACAACCACTTATACTGT CAGCAGTATGCTGAAGTTAAGTATACCCAAGGTGGACTTGAACACCTCGAACTTTCAAGA GAGTATTTTGCACAGGCATTGAAACTGAACACTAGAATATGAGAGCCTTGTTTGGACTTA TTATGTCGCAGTCATATTGCTTCTAATCCAACAGCAGTGCACAACGCACAAGGACACATG AATATGCTAGTTGGGCAGCTAGTCCAATAACAGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014673 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014673.2, NP_055488.1 |
| RefSeq Size | 1273 bp |
| RefSeq ORF | 894 bp |
| Locus ID | 9694 |
| UniProt ID | Q15006 |
| Domains | TPR |
| Protein Families | Protease |
| Gene Summary | Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204770 | EMC2 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 35 (TTC35) |
CNY 2,400.00 |
|
| RC204770L3 | Lenti ORF clone of Human tetratricopeptide repeat domain 35 (TTC35), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204770L4 | Lenti ORF clone of Human tetratricopeptide repeat domain 35 (TTC35), mGFP tagged |
CNY 4,800.00 |
|
| RG204770 | EMC2 (tGFP-tagged) - Human tetratricopeptide repeat domain 35 (TTC35) |
CNY 4,000.00 |
