TTC35 (EMC2) (NM_014673) Human Tagged ORF Clone

CAT#: RC204770

EMC2 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 35 (TTC35)

ORF Plasmid: DDK tGFP



  "NM_014673" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-TTC35 Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms KIAA0103; TTC35
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204770 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAGGTCTCAGAGCTTTACGATGTCACTTGGGAAGAAATGAGAGATAAAATGAGAAAATGGAGAG
AAGAAAACTCAAGAAATAGTGAGCAAATTGTGGAAGTTGGAGAAGAATTAATTAATGAATATGCTTCTAA
GCTGGGAGATGATATTTGGATCATATATGAACAGGTGATGATTGCAGCACTAGACTATGGTCGGGATGAC
TTGGCATTGTTTTGTCTTCAAGAGCTGAGAAGACAGTTCCCTGGCAGTCACAGAGTCAAGCGATTAACAG
GCATGAGATTTGAAGCCATGGAAAGATATGATGATGCTATACAGCTATATGATAGGATTTTACAAGAAGA
TCCAACTAACACTGCTGCAAGAAAGCGTAAGATTGCCATTCGAAAAGCCCAGGGGAAAAATGTGGAGGCC
ATTCGGGAGCTGAATGAGTATCTGGAACAATTTGTTGGAGACCAAGAAGCCTGGCATGAACTTGCAGAAC
TTTACATCAATGAACATGACTATGCAAAAGCAGCCTTTTGTTTAGAGGAACTAATGATGACTAATCCACA
CAACCACTTATACTGTCAGCAGTATGCTGAAGTTAAGTATACCCAAGGTGGACTTGAAAACCTCGAACTT
TCAAGAAAGTATTTTGCACAGGCATTGAAACTGAACAACAGAAATATGAGAGCTTTGTTTGGACTTTATA
TGTCGGCAAGTCATATTGCTTCTAATCCAAAAGCAAGTGCAAAAACGAAAAAGGACAACATGAAATATGC
TAGTTGGGCAGCTAGTCAAATAAACAGAGCTTATCAGTTTGCAGGTCGAAGTAAGAAGGAAACCAAATAT
TCTCTTAAGGCTGTCGAAGACATGTTGGAAACATTGCAGATCACCCAGTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204770 protein sequence
Red=Cloning site Green=Tags(s)

MAKVSELYDVTWEEMRDKMRKWREENSRNSEQIVEVGEELINEYASKLGDDIWIIYEQVMIAALDYGRDD
LALFCLQELRRQFPGSHRVKRLTGMRFEAMERYDDAIQLYDRILQEDPTNTAARKRKIAIRKAQGKNVEA
IRELNEYLEQFVGDQEAWHELAELYINEHDYAKAAFCLEELMMTNPHNHLYCQQYAEVKYTQGGLENLEL
SRKYFAQALKLNNRNMRALFGLYMSASHIASNPKASAKTKKDNMKYASWAASQINRAYQFAGRSKKETKY
SLKAVEDMLETLQITQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014673
ORF Size 891 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014673.5
RefSeq Size 1268 bp
RefSeq ORF 894 bp
Locus ID 9694
UniProt ID Q15006
Domains TPR
Protein Families Protease
MW 34.8 kDa
Gene Summary Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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