CHKL (CHKB) (AK097775) Human Untagged Clone
CAT#: SC126592
(untagged)-ORIGENE UNIQUE VARIANT 1 of Human cDNA FLJ40456 fis, clone TESTI2041664, highly similar to CHOLINE/ETHANOLAMINE KINASE (EC 2.7.1.32)
CNY 4,856.00
CNY 6,270.00
货期*
现货
规格
Product images
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经常一起买 (4)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | CHETK; CHKL; CK; CKB; CKEKB; EK; EKB; MDCMC |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for AK097775, the custom clone sequence may differ by one or more nucleotides
GTCCGAAGGGAGCGGAGCGCAGCCTGGCCTGGGGCCCGGTCGAGCCCGCGCCATGGCGGCCGAGGCGACA GCTGTGGCCGGAAGCGGGGCTGTTGGCGGCTGCCTGGCCAAAGACGGCTTGCAGCAGTCTAAGTGCCCGG ACACTACCCCAAAACGGCGGCGCGCCTCGTCGCTGTCGCGTGACGCCGAGTGCAGCCCGAGGAGCTGAGG GTTTACCCCGTGAGCGGAGGCCTCAGCAACCTGCTCTTCCGCTGCTCGCTCCCGGACCACCTGCCCAGCG TTGGCGAGGAGCCCCGGGAGGTGCTTCTGCGGCTGTACGGAGCCATCTTGCAGGTGAGGGGGTTGTGAGC GCCGCAGCACCAGTGGCTTTAGGGCCTGTCGCTTACGCGATGCGGGTAGTATTGTTCCCGTTGCGCAGTT GAGGACACCGAGGTTCACGGTCTGAGTAACACCTCATTACACCGAAGCCTGGGCCTGTATTCCCAGAGCT TTGGGAGGCTGAGGCGAGAGGATCACTTGAGCACAGGAGTTCGAGACCAGCCTGGACAACATAGTGAGAC CCCCATCTCTAAATAAAAATAGACCAACGCTAAAGCCTGTGCTCCAGAGCCTCCAGGCAATTGGATCAGA AGTCGCAGCTCTGGTGGGAGGAAGGCGAGCCCTCATGTGTGTCCCTGTGCCACTTTGCCTTGGCCCCTTT GCTGTCCATCCTTTTTCAGGGCGTGGACTCCCTGGTGCTAGAAAGCGTGATGTTCGCCATACTTGCGGAG CGGTCGCTGGGGCCCCAGCTGTACGGAGTCTTCCCAGAGGGCCGGCTGGAACAGTACATCCCAGTACGGG CCCAGTCCTACCCTCTCCTCCCCAAGGCACCTCCACCCCCTAACCCTACCCCAGTGCCCAATGTCTGCCT CCACATCCCTCACCCCAAGTAGGGAATTTCCCCCAAAACCCTGACTTCCCCTCATTGCCCCAGCCCTTCC CACTCCTGCCCCAGCCCCATCACCACCCTGATAGCTTCCTGGGTGCAGAGTCGGCCATTGAAAACTCAAG AGCTTCGAGAGCCAGTGTTGTCAGCAGCCATTGCCACGAAGATGGCGCAATTTCATGGCATGGAGATGCC TTTCACCAAGGAGCCCCACTGGCTGTTTGGGACCATGGAGCGGTACCTAAAACAGATCCAGGACCTGCCC CCAACTGGCCTCCCTGAGATGAACCTGCTGGAGATGTACAGCCTGAAGGATGAGATGGGCAACCTCAGGA AGTTACTAGAGTCTACCCCATCGCCAGTCGTCTTCTGCCACAATGACATCCAGGAAGGGAACATCTTGCT GCTCTCAGAGCCAGAAAATGCTGACAGCCTCATGCTGGTGGACTTCGAGTACAGCAGTTATAACTATAGG GGCTTTGACATTGGGAACCATTTTTGTGAGTGGGTTTATGATTATACTCACGAGGAATGGCCTTTCTACA AAGCAAGGCCCACAGACTACCCCACTCAAGAACAGCAGAGGCAAAGAAAGGTGAGACCCTCTCCCAAGAG GAGCAGAGAAAACTGGAAGAAGATTTGCTGGTAGAAGTCAGTCGGTATGCTCTGGCATCCCATTTCTTCT GGGGTCTGTGGTCCATCCTCCAGGCATCCATGTCCACCATAGAATTTGGTTACTTGGACTATGCCCAGTC TCGGTTCCAGTTCTACTTCCAGCAGAAGGGGCAGCTGACCAGTGTCCACTCCTCATCCTGACTCCACCCT CCCACTCCTTGGATTTCTCCTGGAGCCTCCAGGGCAGGACCTTGGAGGGAGGAACAACGAGCAGAAGGCC CTGGCGACTGGGCTGAGCCCCCAAGTGAAACTGAGGTTCAGGAGACCGGCCTGTTCCTGAGTTTGAGTAG GTCCCCATGGCTGGCAGGCCAGAGCCCCGTGCTGTGTATGTAACACAATAAACAAGCTTCTTCTTCC >OriGene 5' read for AK097775 unedited
NCGGTTCAAAATTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGCGGAGCG CAGCCTGGCCTGGGGCCCGGTCGAGCCCGCGCCATGGCGGCCGAGGCGACAGCTGTGGCC GGAAGCGGGGCTGTTGGCGGCTGCCTGGCCAAAGACGGCTTGCAGCAGTCTAAGTGCCCG GACACTACCCCAAAACGGCGGCGCGCCTCGTCGCTGTCGCGTGACGCCGAGCGCCGAGCC TACCAATGGTGCCGGGAGTACTTGGGCGGGGCCTGGCGCCGAGTGCAGCCCGAGGAGCTG AGGGTTTACCCCGTGAGCGGAGGCCTCAGCAACCTGCTCTTCCGCTGCTCGCTCCCGGAC CACCTGCCCAGCGTTGGCGAGGAGCCCCGGGAGGTGCTTCTGCGGCTGTACGGAGCCATC TTGCAGGGCGTGGACTCCCTGGTGCTAGAAAGCGTGATGTTCGCCATACTTGCGGAGCGG TCGCTGGGGCCCCAGCTGTACGGAGTCTTCCCAGAGGGCCGGCTGGAACAGTACATCCCA AGTCGGCCATTGAAAACTCAAGAGCTTCGAGAGCCAGTGTTGTCAGCAGCCATTGCCACG AAGATGGCGCAATTTCATGGCATGGAGATGCCTTTCACCAAGGAGCCCCACTGGCTGTTT GGGACCATGGAGCGGTACCTAAAACAGATCCAGGACCTGCCCCCAACTGGCCTCCCTGAG ATGAACCTGCTGGAGATGTACAGCCTGAAGGATGAGATGGGCAACCTCANGAAGTTACTA GAGTCTACCCATCGNCAGTCGTCTTCTGCCACATGACACCAGGAAGGTANGAGAAGCATC TGAGTCTCCTAACCCAGATGGAGAGCCAGAGGCTCTGGAGTGAGCAGAACTCACCCATTC CCCAGGNACATCTGCTGCTCTCAGAGCCAGAATGCTGACAGCTCATGCTGTGA >OriGene 3' read for AK097775 unedited
NNNAACGTTAAGCTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTT TTTGCGAAAAAAAACTTGTTTATTGTGTTACATACACAGCACGGGGCTCTGGCCTGCCAG CCATGGGGACCTACTCAAACTCAGGGACAGGCCGGTCTCCTGAACCTCAGTTTCACTTGG GGGCTCAGCCCAGTCGCCAGGGCCTTCTGCTCGTTGTTCCTCCCTCCAAGGTCCTGCCCT GGAGGCTCCAGGAGAAATCCAAGGAGTGGGAGGGTGGAGTCAGGATGAGGAGTGGACACT GGTCAGCTGCCCCTTCTGCTGGAAGTAGAACTGGAACCGAGACTGGGCATAGTCCAAGTA ACCAAATTCTATGGTGGACATGGATGCCTGGAGGATGGACCACAGACCCCAGAAGAAATG GGATGCCAGAGCATACCGACTGACTTCTACCAGCAAATCTTCTTCCAGTTTTCTCTGCTC CTCTTGGGAGAGGGTCTCACCTTTCTTTGCCTCTGCCAGGTAATGACGAATAAAATGCAA CTGCTGTTCTTGAGTGGGGTAGTCTGTGGGCCTTGCTTTGTAGAAAGGCCATTCCTCGTG AGTATAATCATAAACCCACTCACAAAAATGGTTCCCAATGTCAAAGCCCCTATAGTTATA ACTGCTGTACTCGAAGTCCACCAGCATGAGGCTGTCAGCATTTTCTGGCTCTGAGAGCAG CAAGATGTTCCCTGGGGGAATGGGGGTGAGGTTCTGCTCACTCCAGAGCCCTCTGGCTCT TCCATCTTGGGTTAAGAGACTCAGATGCCTTCTNCTACCTTCCCGGATGTCATTGTGGCA GAAGACGACTGGCGATGGGGTG |
Restriction Sites | NotI-NotI |
ACCN | AK097775 |
Insert Size | 1650 bp |
OTI Disclaimer | The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required. |
OTI Annotation | This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | AK097775.1 |
RefSeq Size | 1957 bp |
RefSeq ORF | 1957 bp |
Locus ID | 1120 |
Domains | Choline_kinase |
Protein Families | Druggable Genome |
Protein Pathways | Glycerophospholipid metabolism, Metabolic pathways |
Gene Summary | Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009] |
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