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SNF5 (SMARCB1) (NM_003073) Human Untagged Clone
CAT＃: SC126727
SMARCB1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1
CNY 5,488.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BAF47; CSS3; hSNFS; INI1; MRD15; PPP1R144; RDT; RTPS1; Sfh1p; SNF5; SNF5L1; Snr1; SWNTS1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC126727 sequence for NM_003073 edited (data generated by NextGen Sequencing)
ATGATGATGATGGCGCTGAGCAAGACCTTCGGGCAGAAGCCCGTGAAGTTCCAGCTGGAG GACGACGGCGAGTTCTACATGATCGGCTCCGAGGTGGGAAACTACCTCCGTATGTTCCGA GGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGAGAGG AAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGATCACGGA TACACGACTCTAGCCACCAGTGTGACCCTGTTAAAAGCCTCGGAAGTGGAAGAGATTCTG GATGGCAACGATGAGAAGTACAAGGCTGTGTCCATCAGCACAGAGCCCCCCACCTACCTC AGGGAACAGAAGGCCAAGAGGAACAGCCAGTGGGTACCCACCCTGCCCAACAGCTCCCAC CACTTAGATGCCGTGCCATGCTCCACAACCATCAACAGGAACCGCATGGGCCGAGACAAG AAGAGAACCTTCCCCCTTTGCTTTGATGACCATGACCCAGCTGTGATCCATGAGAACGCA TCTCAGCCCGAGGTGCTGGTCCCCATCCGGCTGGACATGGAGATCGATGGGCAGAAGCTG CGAGACGCCTTCACCTGGAACATGAATGAGAAGTTGATGACGCCTGAGATGTTTTCAGAA ATCCTCTGTGACGATCTGGATTTGAACCCGCTGACGTTTGTGCCAGCCATCGCCTCTGCC ATCAGACAGCAGATCGAGTCCTACCCCACGGACAGCATCCTGGAGGACCAGTCAGACCAG CGCGTCATCATCAAGCTGAACATCCATGTGGGAAACATTTCCCTGGTGGACCAGTTTGAG TGGGACATGTCAGAGAAGGAGAACTCACCAGAGAAGTTTGCCCTGAAGCTGTGCTCGGAG CTGGGGTTGGGCGGGGAGTTTGTCACCACCATCGCATACAGCATCCGGGGACAGCTGAGC TGGCATCAGAAGACCTACGCCTTCAGCGAGAACCCTCTGCCCACAGTGGAGATTGCCATC CGGAACACGGGCGATGCGGACCAGTGGTGCCCACTGCTGGAGACTCTGACAGACGCTGAG ATGGAGAAGAAGATCCGCGACCAGGACAGGAACACGAGGCGGATGAGGCGTCTTGCCAAC ACGGCCCCGGCCTGGTAA Clone variation with respect to NM_003073.3 >OriGene 5' read for NM_003073 unedited
AATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGAGGCGCCAG TACCCGGCCCGGTCCGCATTTCGCCTTCCGGCTTCGGTTTCCCTCGGCCCAGCACGCCCC GGCCCCGCCCCAGCCCTCCTGATCCCTCGCAGCCCGGCTCCGGCCGCCCGCCTCTGCCGC CGCAATGATGATGATGGCGCTGAGCAAGACCTTCGGGCAGAAGCCCGTGAAGTTCCAGCT GGAGGACGACGGCGAGTTCTACATGATCGGCTCCGAGGTGGGAAACTACCTCCGTATGTT CCGAGGTTCTCTGTACAAGAGATACCCCTCACTCTGGAGGCGACTAGCCACTGTGGAAGA GAGGAAGAAAATAGTTGCATCGTCACATGGTAAAAAAACAAAACCTAACACTAAGGATCA CGGATACACGACTCTAGCCACCAGTGTGACCCTGTTAAAAGCCTCGGAAGTGGAAGAGAT TCTGGATGGCAACGATGAGAAGTACAAGGCTGTGTCCATCAGCACAGAGCCCCCCACCTA CCTCAGGGAACAGAAGGCCNNNAGGAACAGCCAGTGGGTACCCACCCTGCCCAACAGCTC CCACCACTTAGATGCCGTGCCATGCTCCACAACCATCAACAGGAACCGCATGGGCCGAGA CAAGAGAGAACCTTCCCCCTTTGCTNTGATGACCATGACCCAGCTGTGATCCATGAGAAC GCATCTCAGCCCCGAGTGCTGGTCCCCCATCCGCTGGACATGGAGATCGATGGGCAGAAG CTGCGAGACGCCTTCACCTGGACATGAATGAGAAGTTGATGACGCCTGAGATGTTTTCAG AATCCTCTGTGACGATCTGGATTGAACCCGCTGACGTTTGTGCN >OriGene 3' read for NM_003073 unedited
GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTGTTGAAGAAAATTGAACATGA CCTGTTGCCTTTTATTTAAAAACTGTTACTAGCCCTGCCTGGGGCTCCTATACAAAAACA AAACACAACCTAAAATAAGGTTTCTTCCTGACCCCAAAAACTGGGGAGGGGTAGGGAGGG TGGGNGTCCCCCCCCCCCCCTCCCCCCCCCCCCCCCCCTTCCCTCCCCCCCCCCCCCCCT CCCCCTCCCCCCCCCTTCCCCCTTCTTCCCCCTCTCTTCCCCTTCCCTTCCCCCCCCCCC CCCCCCCCTCTCTTCCCCTCCTCCCCCCCCCCTCCCCCTTCCCTCCTTTCCTCCCCTCCT CCCCCTCTCCCCTCCCCTCCTCTCCCCCCCCCTCCCCCCCCTCCCCCCCTTTCCTCCCCT TCCCCCCCCCCCCCCCCCCCCCTCCCCCCCCCCCTTCTCCCCCCCTCCTCCTCTTCCTCC CCTCCCTCCCCCTCTCCCCCCCCCCTTCCTCTCCCCCTCCCCCTCCCCCCTCCCCCCCTT CCCCTCCGCCCTTCCCCCCCTCCCCTTTTTCCCCCCTTCCCCCCCCCCCCTTCCCCCCCC TCCCCCCTCCCCCCGTTCCCTACCCCATTCCCCCCCCCCCCCCCCCGCTTCCCTTTCCCC CTCCTCCCCCTCCCTTCCCTCTTCCCCCCCCTCCGGTCTTCCCCCCCCTCCATCCCTTCC CCTTTCCCCCCCCCGCCCCCCTCCGTCCTCCCCTCCTCCTCCTCCCCCTCCCCTCCCTTC CCTTCTCCTCTCCCCCCTCCTCTTTTCCCCCCCTCTTCCCCCCGTCCTTCCCCCCGTCCT TCCCCCCGTCCCCCCTTCCCGTCCCCTCCCCCCCCCGTCTTCCCCTCTCCCCCCTTTCCC CCCCTTTCTCCCCCCCCCTG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003073 |
| Insert Size | 1710 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003073.3, NP_003064.2 |
| RefSeq Size | 1717 bp |
| RefSeq ORF | 1158 bp |
| Locus ID | 6598 |
| UniProt ID | Q12824 |
| Domains | SNF5 |
| Protein Families | Transcription Factors |
| Gene Summary | The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (1) encodes isoform a. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Premature termination of SMARCB1 translation may be followed by reinitiation in schwannomatosis-associated schwannomas, but results in absence of SMARCB1 expression in rhabdoid tumors
,Hulsebos, TJ;Kenter, S;Verhagen, WI;Baas, F;Flucke, U;Wesseling, P;,
Acta Neuropathol. April 2014
,PubMed ID 24740647
[SMARCB1]
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Expression of SMARCB1 (INI1) mutations in familial schwannomatosis
,Miriam J. Smith, James A. Walker, Yiping Shen, Anat Stemmer-Rachamimov, James F. Gusella, and Scott R. Plotkin,
Hum. Mol. Genet., Dec 2012; 21: 5239 - 5245.
[SMARCB1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218809 | SMARCB1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1 |
CNY 5,488.00 |
|
| RC218809L1 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC218809L2 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RC218809L3 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC218809L4 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG218809 | SMARCB1 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 (SMARCB1), transcript variant 1 |
CNY 7,088.00 |
