U2AF35 (U2AF1) (AK098266) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FP793; RN; RNU2AF1; U2AF35; U2AFBP
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK098266, the custom clone sequence may differ by one or more nucleotides TTATAATATTTCTTAGTTTGAGCCCAGGTGCGGTGTCTCACGCCTGTAATCACAGCACTTTGGGAGGCCA AGGCAGGCGGATCACTTGAGGTCAGGAGTTCAAGACCAGCCTGCCAACGTGGTGAAACCCTGTCTGTACT AAAAATTAAAAAAAAAAAAAAAAAAATTAGCCGGGCGTGGTGGCAGTCGCCTGTAGTCCCAGCAACTCCA GAGGCTGAGACAGGAGAATCGCTTGAACCCCAGAGGTGGAGGTTGCAGTGAGCTGAGATGGTGCCACTGC ACTCCAGCCTGGGCGACAGAGCCAGACTGCATCTTGTGGGTGTAAAAAAAAAATTTGTAGTTTGAGAGTC AACTTTTTCCTCACAGTTTCTGAAAATGTGGCCCTTTTGATGCTGATTAAAGCTGGTTGTGATTTTAACA CCTTAGTAGCCAGAATCGAGACTGTCATGGGGACATTTAAAATCTCACCACACTTGACACCATTCACAAG TAGCCATGTGGCTCAGTCTCCTGAGTGCTCCTGCAGAGTGCAGCTCTGCCAGGCTTTTCTCTAGATAATC TCGTGTGTGTTACTGTAGCAGTTTGAGTTTAAACACAAGAGTTCACTCGGTTAACATGTTCTAACCAGCA AGATTTCTGTTGTTTGCAGGTGCCGTGAGCGATGTGGAGATGCAGGAACACTATGATGAGTTTTTTGAGG TGAGAGAACTGAACTCTGTGACTGATAGACCTGTTGATCTTCTGTATCTGTTTGGAAGGATTTAAAATGT TTCTCTGCTAATAAACATACATGAGTTATATCTAATGACACTTTGTAGTGTATAACGAGTCTTAAACCAC TGTGGTCTCCCTTCAGGAGGTTTTTACAGAAATGGAGGAGAAGTATGGGGAAGTAGAGGAGATGAACGTC TGTGACAACCTGGGAGACCACCTGGTGGGGAACGTGTACGTCAAGGTAGGAGCCAGGGCGAGACGGCGGC GTGGCCGCTTAGTGTGCCGGGGCCAAGGCCAACAGGCGAAAGCGAGACAGTGGTGCAGCCGCTTAGTGTG CCGGGGCCAAGGCCGACAGAGGATGGGGTGCCTGTCGCCATGCACCTTGAGATCCTGGATGCTCAGGCGC ACAGCAGGGCTCAGGTGCACTGGCTCGGCGCCTACCGGCTGCGTCATATGTGGCGGTGGCCAGCCCACAC GTGCGCCCAGCCAGCAGAACTGAAGGACGGACCGTACACCACAGCCATCGTTATTTCAGTGTAGAATATT TAGTACCTGTTGGTTTAGAAGCTGTGTGGACTAAGGAAAAGTTAGGTTTACTCACATGTTTGGTTTCTTT TTTCTTTTTTAGTGAATAAAACTTTATTTGCCTACACAGCCATATTTGCTCCTTTAACCAGTTGAATTTG AAAAATGAAGGCAACATTTGGCAAAATCTTGGACTATCTTGAAGTTAATCTCATTGAAAATAAAGGATTT TTCAATTATCCTGTAATACTATTCCACCTTACTATACCTGAGTGTGTATATCTCTCTCTGATACATACTT GGATTGGTAATTGAGAAAGTCTTATTAAAGCGTGGATGGCAAGCACTTCTGTTTTTGTCTTTTCAGTTTC GCCGTGAGGAAGATGCGGAAAAGGCTGTGATTGACTTGAATAACCGTTGGTTTAATGGACAGCCGATCCA CGCCGAGCTGTCACCCGTGACGGACTTCAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGAGAATGCACA CGAGGCGGCTTCTGCAACTTCATGCATTTGAAGCCCATTTCCAGAGAGCTGCGGCGGGAGCTGTATGGCC GCCGTCGCAAGAAGTGAGTATTCCTGTGCCCGTTGTTAAGCTCAGTGGCTTCTTTCTCCCCTCACAACCC TCCCCCTTCCAAAAAAAAAAATTTTGGCATAGGTGTCGTTTCTGTTTTTTAAAAGGCAGTGCTTAGAACA TAGTACATATTTTTAAATATCCAAATGTCCTCTTTGGAGAAGAATATTCACCTGCACACAGGTAACTTCA TTATCCAACTTCTCAAGTAGAGATCAGATCTTAGTAATCAAGCAAGAGGGCGGTTAGCTGGGCAGCCGCG GACATGAGGCTCGTGGTGAAAGTGGGCATGCAGTTTATTCTCCCAGATATTACGAAGAAGAAGAATAGAT GTTGTAACCTCCTTTAGATCAGGGATGGCTTCCCTGGGACACATTGGAAGAAGAATTGTCTTGGGTCACA AATAAAATACACTAATGATAGCTGATAACTTTTTTTTTAATTGCAAAAAAAATCTCATAATGTTTTAAGC AAGTTGATGAATTTGTGTTGGGCTGCAGGTTGGACAAGCTTTTGCTTTACATATTTCACATGTCCACTGG GGTTTCTTTTGGTTTTGCTTGTTGGTTTTAGGGCATTTCATTATGTCTTGGGATCGCTGGCATCCTCCCT TCGGGACACTGGCAGAATCAGGCCCAGCGGTCACATCTCTCTGATCCTACATTTAAATGTACGACAGGTC ACAGAGCAGAGAAAATCAAGACGTCAGAATCGAACCGATTGAGTTCTGAGTCTGGAGAGGTTCTCATCCC TCTTCTGTTAAACATGGACTAGTAAATAGATGGGTGGCGATTGTTTTATTGTCAGCATTCAAGAAGCATA AGAGAAAAACAGGCCTGCTCTCCCCAGCCCAAAAACCTGATGGACACAGTAGCTATGAGCGCCTGAAGCG TGGGGTCAGTGAGGGTTGAAGATGGTATGTGTCCAGCTCATTTTGATTATGATTTCCTGTGACCATTTGA GTTGGTTCTAGAAGAAGACAGAAGATGCAAGGTATTACACCTGTTCACTTCTAAGTTGCACATTTTTTTC TCCTGAAACTTTTCACACCTGAAATCAGGGTGTGTCTCAGTCACAGCGTGCAGTCTCTTCAGTGTTTTTC CTTATCAGTGCCGTTGGCTTGAGGAGCTGCGCGTGTTGAGAGAGTGGGTGTGGTTCTGTGCTGCGTGCCG TGGTTTGGATGGCACGGTTTTCTTTATTTTCCTTGGTTGCAGGCATAGATCAAGATCCCGATCCCGGGAG CGTCGTTCTCGGTCTAGAGACCGTGGTCGTGGCGGTGGCGGTGGCGGTGGTGGAGGTGGCGGCGGACGGG AGCGTGACAGGAGGCGGTCGAGAGATCGTGAAAGATCTGGGCGATTCTGAGCCATGCCATTTTTACCTTA TGTCTGCTAGAAAGTGTTGTAGTTGATTGACCAAACCAGTTCATAAGGGGAATTTTTTTTAAAAAACAAC AAAAAAAAAAACATACAAAGATGGGTTTCTGAATAAAATTTGTAGTGAT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK098266 unedited GGGCGGCCCGCGAATTCGCACGAGGCAGGAACACTATGATGAGTTTTTTGAGGAGGTTTT TACAGAAATGGAGGAGAAAGTATGGGGAAGTAGAGGAGATGAACGTCTGTGACAACCTGG GAGACCACCTGGTGGGGAACGTGTACGTCAAGGTAGGAGCCAGGGCGAGACGGCGGCGTG GCCGCTTAGTGTGCCGGGGCCAAGGCCAACAGGCGAAAGCGAGACAGTGGTGCAGCCGCT TAGTGTGCCGGGGCCAAGGCCGACAGAGGATTGGGGTGCCTGTCGCCATGCACCTNTGAG ATCCTGGATGCTCAGGCGCACAGCAGGGGCTCAGGTGCACTGGCTCGGCGCCTACCGGCT GCGTCATATGTGGGCGGTGGCCAGCCCACACGTGCGCCCAGCCAGCAGAACTGAAGGACG GACCGTACACCACAGCCATCGTTATTTCAGTGTAGAATATTTAGTACCTGGTTGGTTTAG AAGCTGTGTGGACTAAGGAAAAGTTAGGTTTACTCACATGTTTGGTTTCTTTTTTCTTTT TTAGTGAATAAAACTTTATTTGCCTACACAGCCATATTTGCTCCTTTAACCAGTTGAATT TGAAAAATGAAGGCAACATTTGGCAAAATCTTGGACTTCTTTTGAAGTTAATCTCATTGA AAATAAAAGGATTTTTCAATTATCCTGGTATACTATTCCACCTTACTATACCTGGAAGTG GGTATATCTCTCTCTGATACATACTTGGGATGGGGATGGGAAAAAGTCTTATTAAGCGTG GGATGGCAGCACTTCTGGGTTTTGCCTTTTCAGTTCCCCCGGGAGGAAAATGCGGAAAAG CTTGGGATGACTTGATAAACCGTGGGTTTAATGACGGNCGATCCA
Restriction Sites	NotI-NotI
ACCN	AK098266
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK098266.1
RefSeq Size	3339 bp
RefSeq ORF	3339 bp
Locus ID	7307
Protein Families	Stem cell - Pluripotency
Protein Pathways	Spliceosome
Gene Summary	This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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