Apc1 (ANAPC1) (NM_022662) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APC1; MCPR; TSG24
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_022662, the custom clone sequence may differ by one or more nucleotides ATGTCGAACTTCTATGAAGAAAGGACAACGATGATTGCAGCAAGGGATTTGCAGGAATTTGTTCCTTTTG GTCGAGACCACTGCAAGCACCACCCTAATGCTTTGAACCTTCAACTTCGCCAGCTGCAGCCAGCTTCTGA ATTATGGTCTTCTGATGGTGCTGCTGGCTTGGTGGGATCCCTTCAGGAGGTTACAATCCACGAGAAACAG AAGGAAAGCTGGCAGTTAAGGAAAGGAGTAAGTGAAATTGGAGAAGATGTGGACTATGATGAGGAACTCT ATGTTGCTGGAAATATGGTGATATGGAGCAAAGGAAGTAAAAGCCAGGCATTGGCAGTTTATAAAGCATT TACAGTTGACAGTCCTGTTCAGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAGTCTGAAAAG GCCTACAGTAGCAATGAAGTAGAAAAATGCATATGTATATTGCAAAGCTCATGTATTAACATGCATAGCA TAGAAGGAAAGGATTACATAGCTTCATTACCATTTCAGGTTGCAAATGTTTGGCCCACTAAATATGGATT GCTGTTTGAACGAAGCGCTTCTTCACATGAAGTACCTCCAGGTTCACCCAGAGAACCTTTACCTACTATG TTCAGCATGCTGCACCCACTAGATGAAATAACTCCACTTGTTTGTAAATCTGGAAGTCTTTTTGGTTCAT CACGGGTGCAATATGTTGTAGATCATGCAATGAAAATTGTTTTCCTCAATACTGACCCCTCTATTGTAAT GACTTATGATGCTGTTCAAAATGTGCATTCTGTGTGGACTCTCCGGAGAGTCAAATCAGAGGAAGAGAAT GTTGTTTTAAAGTTCTCTGAACAGGGGGGAACCCCACAGAATGTGGCCACTAGCAGCTCCCTCACAGCAC ATCTCAGAAGCCTCTCCAAAGGAGATTCCCCTGTGACTTCACCTTTCCAGAATTACTCCTCCATTCACAG CCAGAGTCGCTCAACCTCATCACCCAGTCTACATTCTCGCTCACCTTCTATTTCCAACATGGCAGCTCTA AGTCGTGCTCATTCTCCTGCGTTAGGAGTGCACTCTTTTTCAGGGGTGCAAAGGTTCAACATTTCAAGCC ATAATCAGTCTCCAAAGAGACATAGTATTTCTCATTCTCCAAATAGTAATTCTAATGGCTCCTTTCTTGC ACCAGAAACGGAGCCAATTGTTCCTGAACTGTGTATTGACCATTTGTGGACAGAAACGATTACTAATATA AGAGAGAAAAATTCACAAGCCTCAAAAGTGTTTATTACATCTGACCTATGTGGGCAAAAGTTCCTGTGCT TTTTAGTAGAGTCCCAGCTCCAGTTACGCTGTGTAAAGTTTCAAGAGAGTAATGATAAAACCCAGCTCAT CTTTGGTTCAGTGACCAACATACCAGCAAAGGATGCAGCACCAGTGGAGAAAATAGACACCATGCTGGTC TTGGAAGGCAGTGGAAACCTGGTGCTATACACAGGAGTGGTTCGGGTGGGAAAGGTTTTTATTCCTGGAC TGCCAGCTCCCTCTCTGACGATGTCCAACACAATGCCTCGGCCCAGTACTCCACTAGATGGCGTTAGTAC TCCAAAGCCTCTTAGTAAACTCCTTGGATCATTGGACGAGGTTGTTCTGTTGTCCCCAGTTCCAGAACTG AGGGATTCTTCAAAACTTCATGATTCTCTCTATAATGAGGATTGTACTTTCCAACAGCTTGGAACTTACA TTCATTCTATCAGAGATCCTGTCCATAACAGAGTCACCCTGGAACTGAGTAATGGCTCCATGGTTAGGAT CACTATTCCTGAAATTGCCACCTCTGAGTTAGTACAAACGTGTTTGCAAGCAATTAAGTTTATCCTGCCA AAAGAAATAGCAGTTCAGATGCTTGTCAAGTGGTACAATGTCCACAGTGCTCCAGGAGGACCCAGTTATC ACTCAGAGTGGAATTTATTTGTGACTTGTCTCATGAACATGATGGGTTATAACACAGACCGCTTAGCATG GACTAGAAATTTTGACTTTGAAGGATCACTTTCTCCTGTCATTGCGCCCAAAAAAGCAAGGCCTTCCGAG ACTGGATCTGATGATGACTGGGAATATTTACTAAATTCAGACTACCACCAGAATGTTGAGTCTCATCTTT TGAACAGATCTTTATGTCTGAGTCCTTCAGAAGCTTCACAGATGAAGGATGAGGATTTTTCACAGAATCT CAGTCTGGATTCTTCTACACTTCTCTTTACTCACATACCTGCAATTTTTTTCGTTCTTCACCTTGTGTAT GAGGAGCTTAAGTTGAATACTCTAATGGGAGAAGGAATTTGTTCACTTGTTGAACTTCTCGTTCAGTTGG CAAGGGACTTAAAATTGGGGCCTTATGTAGATCATTACTATAGAGACTACCCAACGCTTGTCAGAACTAC TGGACAAGTGTGCACAATTGATCCAGGTCAAACAGGATTTATGCATCATCCATCATTTTTTACGTCTGAG CCACCAAGTATTTATCAGTGGGTGAGTTCTTGTCTGAAGGGTGAAGGAATGCCACCTTATCCTTACCTCC CTGGAATCTGTGAAAGAAGCAGACTTGTAGTCTTGAGTATTGCACTGTACATACTTGGTGATGAGAGCTT GGTTTCTGATGAATCCTCACAGTATTTAACCAGAATAACTATAGCCCCCCAGAAGTTGCAAGTAGAACAA GAGGAAAACAGGTTTAGTTTCAGGCATTCTACATCTGTTTCTAGTCTAGCTGAAAGATTGGTTGTCTGGA TGACTAATGTAGGATTCACTTTAAGAGATTTGGAAACTCTTCCCTTTGGAATTGCTCTTCCCATCAGAGA TGCAATTTATCACTGTCGTGAGCAGCCTGCCTCAGACTGGCCAGAAGCTGTCTGTCTCTTGATTGGACGT CAGGATCTTTCCAAGCAGGCCTGCGAAGGAAACTTACCCAAAGGGAAGTCTGTGCTCTCATCAGATGTTC CTTCAGGAACAGAAACTGAGGAGGAAGATGACGGCATGAATGACATGAATCACGAGGTCATGTCATTAAT ATGGAGTGAAGATTTAAGGGTGCAGGATGTGCGAAGGCTTCTTCAGAGTGCGCATCCTGTCCGTGTCAAC GTAGTGCAGTACCCAGAGCTCAGTGACCACGAGTTCATCGAGGAAAAGGAAAACAGATTGCTCCAATTGT GTCAGCGAACTATGGCTCTTCCTGTAGGACGAGGAATGTTTACCTTGTTTTCGTACCATCCTGTTCCAAC AGAGCCATTGCCTATTCCTAAATTGAATCTGACTGGGCGTGCCCCTCCTCGGAACACAACAGTAGACCTT AATAGTGGAAACATCGATGTGCCTCCCAACATGACAAGCTGGGCCAGCTTTCATAATGGTGTGGCTGCTG GCCTGAAGATAGCTCCTGCCTCCCAGATCGACTCAGCTTGGATTGTTTACAATAAGCCCAAGCATGCTGA GTTGGCCAATGAGTATGCTGGCTTTCTCATGGCTCTGGGTTTGAATGGGCACCTTACCAAGCTGGCGACT CTCAATATCCATGACTACTTGACCAAGGGCCATGAAATGACAAGCATTGGACTGCTACTTGGTGTTTCTG CTGCAAAACTAGGCACCATGGATATGTCTATTACTCGGCTTCTTAGCATTCACATTCCTGCTCTCTTACC CCCAACGTCCACAGAGCTGGATGTTCCTCACAATGTCCAAGTGGCTGCAGTGGTTGGCATTGGCCTTGTA TATCAAGGGACAGCTCACAGACATACTGCAGAAGTCCTGTTGGCTGAGATAGGACGGCCTCCTGGTCCTG AAATGGAATACTGCACTGACAGAGAGTCATACTCCTTAGCTGCTGGCTTGGCCCTGGGCATGGTCTGCTT GGGGCATGGCAGCAATTTGATAGGTATGTCTGATCTCAATGTGCCTGAGCAGCTCTATCAGTACATGGTT GGAGGACATAGGCGCTTTCAAACAGGAATGCATAGGGAGAAACATAAATCACCAAGTTATCAAATCAAAG AAGGAGATACCATAAATGTGGATGTGACTTGTCCAGGTGCTACTCTAGCTTTGGCTATGATCTACTTAAA AACCAATAACAGATCTATTGCAGATTGGCTCCGAGCCCCTGACACCATGTATTTGTTGGACTTTGTGAAG CCAGAATTTCTCTTGCTTAGGACACTTGCTCGATGCCTGATTTTGTGGGATGATATTTTACCAAATTCCA AGTGGGTTGACAGCAATGTTCCTCAAATTATAAGAGAAAATAGTATCTCTCTCAGTGAAATCGAATTGCC GTGCTCAGAGGATTTGAATTTGGAAACTTTGTCCCAAGCACATGTCTACATAATTGCAGGAGCCTGCTTG TCTCTGGGTTTTCGATTTGCTGGCTCAGAAAACTTATCAGCATTTAACTGTTTGCATAAATTTGCCAAAG ATTTTATGACTTATTTGTCCGCACCTAATGCTTCTGTTACAGGTCCTCATAACCTAGAAACTTGTCTGAG CGTGGTGCTGCTGTCTCTCGCCATGGTCATGGCTGGCTCAGGAAACCTAAAGGTTTTGCAGCTTTGTCGC TTCTTACACATGAAAACGGGTGGTGAAATGAACTATGGTTTTCACTTAGCCCACCACATGGCCCTTGGAC TTCTATTTTTGGGAGGAGGAAGGTACTCTTTGAGCACATCAAATTCTTCCATTGCCGCTCTTCTCTGTGC CCTTTATCCGCACTTCCCAGCTCACAGCACTGACAACCGGTATCATCTCCAGGCTCTCCGGCACCTCTAT GTGCTGGCCGCGGAGCCCAGGCTTCTAGTGCCTGTGGATGTGGACACAAACACGCCCTGCTATGCCCTCT TAGAAGTTACCTACAAGGGCACTCAGTGGTATGAACAAACCAAAGAAGAATTGATGGCTCCTACCCTTCT TCCAGAACTCCATCTTTTAAAGCAGATTAAAGTAAAAGGCCCAAGATACTGGGAACTGCTCATAGATTTA AGCAAAGGAACACAACACTTGAAGTCCATCCTTTCCAAGGATGGGGTTTTATATGTTAAACTCCGGGCGG GTCAGCTCTCCTACAAAGAAGATCCAATGGGATGGCAAAGTTTGTTGGCTCAGACTGTTGCTAACAGGAA CTCTGAAGCCCGGGCTTTCAAGCCAGAAACAATCTCAGCATTCACTTCTGATCCAGCACTTCTGTCATTT GCTGAATATTTCTGCAAGCCAACTGTGAACATGGGTCAGAAACAGGAAATTCTGGATCTCTTTTCTTCAG TACTCTATGAATGTGTTACCCAGGAGACCCCAGAGATGTTGCCTGCATACATAGCAATGGATCAGGCTAT AAGAAGACTTGGGAGAAGAGAAATGTCTGAGACTTCTGAACTTTGGCAGATAAAGTTGGTGTTAGAGTTT TTCAGCTCCCGAAGCCATCAGGAGCGGCTGCAGAACCACCCTAAGCGGGGGCTCTTTATGAACTCGGAAT TCCTCCCTGTTGTGAAGTGCACCATTGATAATACCCTGGACCAGTGGCTACAAGTCGGGGGTGATATGTG TGTGCACGCCTACCTCAGCGGGCAGCCCTTGGAGGAATCACAGCTGAGCATGCTGGCCTGCTTCCTCGTC TACCACTCTGTGCCAGCTCCACAGCACCTGCCACCTATAGGACTAGAAGGGAGCACAAGCTTTGCTGAAC TGCTCTTCAAATTTAAGCAGCTAAAAATGCCAGTGCGAGCTTTGCTGAGATTGGCTCCTTTGCTTCTTGG AAATCCACAGCCAATGGTGATGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_022662 unedited AACAACCTCATGTTAGGGCGGCCGCGCATTCGGCACGAGGGGACGCGTTCTATTGAACGT CTCGCACGCCTTCCTGCCATTAGCACTCGAGCCCGCTGCTGTTGCCCGTTCTTCCTCCGG AATAGGGGAGGGAGAGGGAATGAGAAGCTGCTGCGGCCCAAGAGTCACTGTGAAGGACCC CGCCGCTGCCCTCGGGCCTCCTCTGCCCCTGCGCCTCCGGGGAGCAGCCGGGGCTCGCCG CGCCTGACGCGTCCCGAGTTATACAGAAATAATGTTGATATTTGGAACCCATGTCGAACT TCTATGAAGAAAGGACAACGATGATTGCAGCAAGGGATTTGCAGGAATTTGTTCCTTTTG GTCGAGACCACTGCAAGCACCACCCTAATGCTTTGAACCTTCAACTTCGCCAGCTGCAGC CAGCTTCTGAATTATGGTCTTCTGATGGTGCTGCTGGCTTGGTGGGATCCCTTCAGGAGG TTACAATCCACGAGAAACAGAAGGAAAGCTGGCAGTTAAGGAAAGGAGTAAGTGAAATTG GAGAAGATGTGGACTATGATGAGGAACTCTATGTTGCTGGAAATATGGTGATATGGAGCA AAGGAAGTAAAAGCCAGGCATTGGCAGTTTATAAAGCATTTACAGTTGACAGTCCTGTTC AGCAGGCATTGTGGTGTGACTTCATTATATCACAGGATAAAGTCTGAAAACGCCTACAGT TGCAATGAAGTAGAAAATGCATATGTATATTGCAAAGCTCATGTTTTAACATGCCTAGCC TANAAGGAAAGGATTACATTAGCTTCATAACCATTTCAGGTTGCCAAATGTTTGCGCCAC TAAATATGGATTGCTGGTTGAACCGAGCGCTTTTCACATGNAGTACCTCCAGTTCACCCA GTCTTTTTGGTTCATCACGGTGCCATAAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_022662 unedited NNTTTTAGCTTGCACCGCGGCCCGCAATCTATATCGAGTTTTTTTTTTTTTTTTTTTTTT GGAAACAAACCAAAAACTTTATTTACAAAAGTAAATTTTAACTTGCTTTTATATGTCATA TACCGTTAATGATGACAGCAACAGATTTAAAATACATTGAGGTTTGTGCAGCTCATTTCC CCCTAGTTATACCATAAAACTTTATAAACATTGCTTTACCTTTGATGTTTGGTCACCTTT GTTGTGCATAAGTCACGTTTCATGGTAGGTTCACCGCCAGACACAGTCACATCACCATTG GCTGTGGATTTCCAAGAAGCAAAGGAGCCAATCTCAGCACAGCTCGCACTGGCATTTTTA GCTGCTTAAATTTGAAGAGCAGTTCACCAAAGCTTGTGCTCCCTTCTAGTTCTATAGGTG CGACGCGCTGCGGCCTTGGCACATATTGGTACACCAGCAACCCTGCCCCCCTGCTCACCT GTGATCCCTCCAAGGCTGCCCCCTGTAGCAGTCCCCCACACCCCTATTACCCCCTACTTG TTTCCACTGGTCCCGTGCATTATTACCGGCGCCCCTTATATCTTCCCGTCATTCCTACCT TAAAAGATTCCCCGCTCTACGTGGTTTTCCAACTTTCTTCTATGGTCTCCTATCCCCAAC ACTTACCACCCTTTTTCCCCGTTCCATTCCCCCCCCCCTTAATTTTCTCTTTCTCAACAC TCATAAACCCGATTTATCCTCCGTCAGCATTCACCACTCTGTGGTTTTTCGGCCCCCTTC ACCCTCCAATACTATCCAAACACCCTCCCGCTCTCCCCTTTCTCTTTCCCGCCCCCCATC TACTCCTCTCACCCTTTACAACTCTTACAAATCTCCCCATCCCACACACATTCCCCCTAT CCCCCGACTCACACTCCCGNCCTTCCTC
Restriction Sites	NotI-NotI
ACCN	NM_022662
Insert Size	5600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022662.2, NP_073153.1
RefSeq Size	7753 bp
RefSeq ORF	5835 bp
Locus ID	64682
UniProt ID	Q9H1A4
Domains	PC_rep
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
Gene Summary	This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]

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