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SGSH (NM_000199) Human Untagged Clone
CAT＃: SC127166
SGSH (untagged)-Human N-sulfoglucosamine sulfohydrolase (SGSH)
CNY 3,744.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSS; MPS3A; SFMD |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127166 sequence for NM_000199 edited (data generated by NextGen Sequencing)
ATGAGCTGCCCCGTGCCCGCCTGCTGCGCGCTGCTGCTAGTCCTGGGGCTCTGCCGGGCG CGTCCCCGGAACGCACTGCTGCTCCTCGCGGATGACGGAGGCTTTGAGAGTGGCGCGTAC AACAACAGCGCCATCGCCACCCCGCACCTGGACGCCTTGGCCCGCCGCAGCCTCCTCTTT CGCAATGCCTTCACCTCGGTCAGCAGCTGCTCTCCCAGCCGCGCCAGCCTCCTCACTGGC CTGCCCCAGCATCAGAATGGGATGTACGGGCTGCACCAGGACGTGCACCACTTCAACTCC TTCGACAAGGTGCGGAGCCTGCCGCTGCTGCTCAGCCAAGCTGGTGTGCGCACAGGCATC ATCGGGAAGAAGCACGTGGGGCCGGAGACCGTGTACCCGTTTGACTTTGCGTACACGGAG GAGAATGGCTCCGTCCTCCAGGTGGGGCGGAACATCACTAGAATTAAGCTGCTCGTCCGG AAATTCCTGCAGACTCAGGATGACCGGCCTTTCTTCCTCTACGTCGCCTTCCACGACCCC CACCGCTGTGGGCACTCCCAGCCCCAGTACGGAACCTTCTGTGAGAAGTTTGGCAACGGA GAGAGCGGCATGGGTCGTATCCCAGACTGGACCCCCCAGGCCTACGACCCACTGGACGTG CTGGTGCCTTACTTCGTCCCCAACACCCCGGCAGCCCGAGCCGACCTGGCCGCTCAGTAC ACCACCGTCGGCCGCATGGACCAAGGAGTTGGACTGGTGCTCCAGGAGCTGCGTGACGCC GGTGTCCTGAACGACACACTGGTGATCTTCACGTCCGACAACGGGATCCCCTTCCCCAGC GGCAGGACCAACCTGTACTGGCCGGGCACTGCTGAACCCTTACTGGTGTCATCCCCGGAG CACCCAAAACGCTGGGGCCAAGTCAGCGAGGCCTACGTGAGCCTCCTAGACCTCACGCCC ACCATCTTGGATTGGTTCTCGATCCCGTACCCCAGCTACGCCATCTTTGGCTCGAAGACC ATCCACCTCACTGGCCGGTCCCTCCTGCCGGCGCTGGAGGCCGAGCCCCTCTGGGCCACC GTCTTTGGCAGCCAGAGCCACCACGAGGTCACCATGTCCTACCCCATGCGCTCCGTGCAG CACCGGCACTTCCGCCTCGTGCACAACCTCAACTTCAAGATGCCCTTTCCCATCGACCAG GACTTCTACGTCTCACCCACCTTCCAGGACCTCCTGAACCGCACCACAGCTGGTCAGCCC ACGGGCTGGTACAAGGACCTCCGTCATTACTACTACCGGGCGCGCTGGGAGCTCTACGAC CGGAGCCGGGACCCCCACGAGACCCAGAACCTGGCCACCGACCCGCGCTTTGCTCAGCTT CTGGAGATGCTTCGGGACCAGCTGGCCAAGTGGCAGTGGGAGACCCACGACCCCTGGGTG TGCGCCCCCGACGGCGTCCTGGAGGAGAAGCTCTCTCCCCAGTGCCAGCCCCTCCACAAT GAGCTGTGA Clone variation with respect to NM_000199.3 >OriGene 5' read for NM_000199 unedited
ACGACTTCCTATAGGGCGGCCGCGAATTCGGCACGAGGGAGAGCCGGTTCCGGATCCCGA TCCCGAGTCCGAGCCGCCGCCGCCATGAGCTGCCCCGTGCCCGCCTGCTGCGCGCTGCTG CTAGTCCTGGGGCTCTGCCGGGCGCGTCCCCGGAACGCACTGCTGCTCCTCGCGGATGAC GGAGGCTTTGAGAGTGGCGCGTACAACAACAGCGCCATCGCCACCCCGCACCTGGACGCC TTGGCCCGCCGCAGCCTCCTCTTTCGCAATGCCTTCACCTCGGTCAGCAGCTGCTCTCCC AGCCGCGCCAGCCTCCTCACTGGCCTGCCCCAGCATCAGAATGGGATGTACGGGCTGCAC CAGGACGTGCACCACTTCAACTCCTTCGACAAGGTGCGGAGCCTGCCGCTGCTGCTCAGC CAAGCTGGTGTGCGCACAGGCATCATCGGGAAGAAGCACGTGGGGCCGGAGACCGTGTAC CCGTTTGACTTTGCGTACACGGAGGAGAATGGCTCCGTCCTCCAGGTGGGGCGGAACATC ACTAGAATTAAGCTGCTCGTCCGGAAATTCCTGCAGACTCAGGATGACCGGCCTTTCTTC CTCTACGTCGCCTTCCACGACCCCCACCGCTGTGGGCACTCCCAGCCCCAGTACGGAACC TTCTGTGAGAAGTTTGGCAACGGAGAGAGCGGCATGGGTCGTATCCCAGACTGGACCCCC CAGGCCTACGACCCACTGGACGTGCTGGTGCCTTACTTCGTCCCCACACCCGGCAGCCCG AGCCGACCTGGCCGCTCAGTCACCACCGTCGGCCGATGGACCAANGAGTGNACTGGTGCT NCAGAGCTGGTGACGCCCGTGTCCTGACGACCACTGGTGATCTTACGTCCGACACGGGAT CCCTTCCCAGCGGAGACCAACTGACTGGNCGCCCTGCTGACCTTACTGTTGCTNCCCGAG CACCATAGCTGGGCAGTACGAGGCCACTGACTCCN >OriGene 3' read for NM_000199 unedited
TATGGACCGCGNGCCGCAATCTAGAGTCGAGTTTTTNCCTTTTTTTTTTTTTTTTTTTTT TTTAAACTTCTGTATATTTTACTAAAAAAAAACTTTTACAATAGCTGGCCTGGGGCCTCC TCCAGCCCACCCCAGTATGGAGTGATAGGGAGGGAAGGGCAAGGGGAGCCCACCTACTCA AGGAAGCGCCCTCTCCTTCGAGGGGTGTCCAGGCCGAGGGGTGTCCAGGCCGGGCTTCTG CTCCCGAGGTGGGTGGAGGCAGGGCAGGAACGGCACATTCTCCCAGCAACGCCGACGTCA TCCAAGAATTAACCCAAGCCAGAGGACGGGCATCGCCACCCAGCAACGCCAGTGTCACCG AAAAATTAGCCCAAGCCAGAGGACGGGCATTGCCACCCAGCAACGCCAGTGTCACCGAAG AATTAACCCAAGCCAGAGGACGGGCATCACCACCCAGCAATGCCAGTGTCACCGAAGAAT TAACCCAAGCCAGAGGACGGGCATCGCCATGCCTTCATCTTCGGACACTCTCAAAAAAGA TAAGCTTCTGCCCAGAAACACCACAGGTTCAAGCTCATCACTCAGGGAGCCTGCTGCCAA TCAGCTGAAACCTGCCTGGGGAACAGGGACTTGACCATGGGGCAAAACAGAAGAAGCAGA AACCTGCCCAAAGGTCGCTCAAAGGCTTCCTCTAGGCCAGACGCTGGTAAGAGCCACGGC GCCGTGCTCCCAGGTGAGTGTCGGTGGGACCTGCGTACTGCCCCAGCGGAACCGGAAGCC CCTGCCTGCTCTTTGGGAAGGTTCAGAAGTATCTGTGGCTGCCCAAGCCTGAAGAGGCCT AAGGCCTCCATTTGAAGTCCCCAACCAAGCAAGAACAAGACTCCCTGAATGGGCTGGGGC CTGCCTGTCCGAAACACTTTGTTAAAATTCCCGGCTGGAATGGTTACACCAGAACAATGG CTCCTGCATGAAGAAGCCCN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000199 |
| Insert Size | 2880 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000199.2, NP_000190.1 |
| RefSeq Size | 2740 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 6448 |
| UniProt ID | P51688 |
| Domains | Sulfatase |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycosaminoglycan degradation, Lysosome, Metabolic pathways |
| Gene Summary | This gene encodes the enzyme sulfamidase; one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with the lysosomal storage disease mucopolysaccaridosis IIIA, also known as Sanfilippo syndrome A, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jun 2017] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208270 | SGSH (Myc-DDK-tagged)-Human N-sulfoglucosamine sulfohydrolase (SGSH) |
CNY 3,736.00 |
|
| RC208270L1 | Lenti ORF clone of Human N-sulfoglucosamine sulfohydrolase (SGSH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208270L2 | Lenti ORF clone of Human N-sulfoglucosamine sulfohydrolase (SGSH), mGFP tagged |
CNY 5,890.00 |
|
| RC208270L3 | Lenti ORF clone of Human N-sulfoglucosamine sulfohydrolase (SGSH), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208270L4 | Lenti ORF clone of Human N-sulfoglucosamine sulfohydrolase (SGSH), mGFP tagged |
CNY 5,890.00 |
|
| RG208270 | SGSH (tGFP-tagged) - Human N-sulfoglucosamine sulfohydrolase (SGSH) |
CNY 5,336.00 |
|
| SC322658 | SGSH (untagged)-Human N-sulfoglucosamine sulfohydrolase (SGSH) |
CNY 3,744.00 |
