Glucokinase (GCK) (BC001890) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATP:D-hexose 6-phosphotransferase; GK; GLK; glucokinase; glucokinase (hexokinase 4); hexokinase D, pancreatic isozyme; HHF3; HK4; HKIV; HXKP; MODY2; OTTHUMP00000024521; OTTHUMP00000159308
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC127236 sequence for BC001890 edited (data generated by NextGen Sequencing) ATGCTGGACGACAGAGCCAGGATGGAGGCCGCCAAGAAGGAGAAGGTAGAGCAGATCCTG GCAGAGTTCCAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAG ATGGACCGCGGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACC TACGTGCGCTCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGT GGCACTAACTTCAGGGTGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGC GTGAAGACCAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAG ATGCTCTTCGACTACATCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAA CACAAGAAGCTGCCCCTGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGAT AAGGGCATCCTTCTCAACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAAT GTCGTGGGGCTTCTGCGAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTG GCAATGGTGAATGACACGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAGTGC GAGGTCGGCATGATCGTGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAGAAT GTGGAGCTGGTGGAGGGGGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCCTTC GGGGACTCCGGCGAGCTGGACGAGTTCCTGCTGGAGTATGACCGCCTGGTGGACGAGAGC TCTGCAAACCCCGGTCAGCAGCTGTATGAGAAGCTCATAGGTGGCAAGTACATGGGCGAG CTGGTGCGGCTTGTGCTGCTCAGGCTCGTGGACGAAAACCTGCTCTTCCACGGGGAGGCC TCCGAGCAGCTGCGCACACGCGGAGCCTTCGAGACGCGCTTCGTGTCGCAGGTGGAGAGC GACACGGGCGACCGCAAGCAGATCTACAACATCCTGAGCACGCTGGGGCTGCGACCCTCG ACCACCGACTGCGACATCGTGCGCCGCGCCTGCGAGAGCGTGTCTACGCGCGCTGCGCAC ATGTGCTCGGCGGGGCTGGCGGGCGTCATCAACCGCATGCGCGAGAGCCGCAGCGAGGAC GTAATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCCAGCTTCAAG GAGCGGTTCCATGCCAGCGTGCGCAGGCTGACGCCCAGCTGCGAGATCACCTTCATCGAG TCGGAGGAGGGCAGTGGCCGGGGCGCGGCCCTGGTCTCGGCGGTGGCCTGTAAGAAGGCC TGTATGCTGGGCCAGTGA Clone variation with respect to BC001890.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001890 unedited CCCGCCGATANGGTAANTGTNAAACGATTTACTATAGGCGGCCGCGNAATTCGCACGAGG GAATCAGGNAAATGCCGAGCGGCGCCTGAGCCCCAGGAAGCAGGCTAGGATGTGAGAGAC ACAGTCACCTGCAGCCTAATTACTCAAAAGCTGTCCCCAGGTCACAGAAGGGAGAGGACA TTTCCCACTGAATCTGTCTGAAGGACACTAAGCCCCACAGCTCAACACAACCAGGAGAGA AAGCGCTGAGGACGCCACCCAAGCGCCCAGCAATGGCCCTGCCTGGAGAACATCCAGGCT CAGTGAGGAAGGGTCCAGAAGGGAATGCTTGCCGACTCGTTGGAGAACAATGAAAAGGAG GAAACTGTGACTGAACCTCAAACCCCAAACCAGCCCGAGGAGAACCACATTCTCCCAGGG ACCCAGGGCGGGCCGTGACCCCTGCGGCGGAGAAGCCTTGGATATTTCCACTTCAGAAGC CTACTGGGGAAGGCTGAGGGGTCCCAGCTCCCCACGCTGGCTGCTGTGCAGATGCTGGAC GACAGAGCCAGGATGGAGGCCGCCAAGAAGGAGAAGGTAGAGCAGATCCTGGCAGAGTTC CAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATGGACCGC GGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTACGTGCGC TCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGTGTGGCACTAAC TTCANGGTGATGCTGGTNGAAGTGTGAAAGGTNGAGAGGGGCAGTGGAGCGTGAAGACAA ACACCAGAGTACTCCATCCCCCGAGACGCATGACCGGCACTGCTGAGATGCTCTTCGATC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC001890 unedited CGTTGCCTTCATTGGACGATTGGCACCTTGCCAGGNCCAGGATAGCACTGGGGNAGTGGG TCACAGGGCATTGCCACCCGGGTATCTGTTCAGGAAAACAGCTATGACCGCGGCCGCAAT CTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTAAAACTTTTAACATTTTTAATTAAAAA ACCCACACAATCTTATGTTGGGCATGGAGAGGGTGGCATGTCATATGATGGGGTTGTCCC TTGGGGTGTGCTGAATGCTGCTTGGGGTTTCTTCCTGAGCCAGCGCTATGGGAGCTGAAT ATGTAGGGCTGGCCCTTCTCCCCTTTGTGGGCCCCTTGGGGCAGAAGCTTGGGGGCTCTG TCTCTTGCACCTGCTGCGAGGGTCCATCCCAGAATCACAAGCCACTCAGTGATGGTATGG GAGAGAAAAGGTCTCTGGGGGTATTCAAAGCTTGGGACACCTCCTCCATGGAGCCCCCTC CCATTGCTCCCTGGGCCTCTTGAGCCTCAGGCAAGAAAATGGCCAGGACTGGGTGATGAG CAGCCCCTGGCTGCCCTTTTGGCCCAAGTCTAGGTCTGGTCAAGCTGTTGGAGCTGCCTC CCCACACAGGATGAGTTCCCAGGGAAGTGAGGCCACAGCAGGATCCAGAGGGCACCCCTT CCCAGAGCCTGGGCCCAGGCGAGGCCAACAGCTCTGACAGTGTGGAAATGCATCAAAGTC CTGAGTGAGCAACTCCCTTCTGGGAAATTGATTCCAGCGAGAAAGGTGGGGGGGCTTCCA GAGTCTCTGTTCCTGCTCCTGCCGCACCCCCGTTAGGGGCCTGGGGCTGTCCCACCGAAA ACTGATGGAAGAGCCCCTCTGTCCACGTTTTTTGGGAAAANNNNCTTTGGTCCTGAAAGG GCAAGCCTCCTGCCCCGCCAT
Restriction Sites	Please inquire
ACCN	BC001890
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001890.1, AAH01890.1
RefSeq Size	2748 bp
Locus ID	2645
Protein Families	Druggable Genome
Protein Pathways	Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus
Gene Summary	This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]

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