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Serine Palmitoyltransferase (SPTLC2) (NM_004863) Human Untagged Clone
CAT＃: SC127529
SPTLC2 (untagged)-Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2)
CNY 4,192.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hLCB2a; HSN1C; LCB2; LCB2A; NSAN1C; SPT2 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127529 sequence for NM_004863 edited (data generated by NextGen Sequencing)
ATGCGGCCGGAGCCCGGAGGCTGCTGCTGCCGCCGCACGGTGCGGGCGAATGGCTGCGTG GCGAACGGGGAAGTACGGAACGGGTACGTGAGGAGCAGCGCTGCAGCCGCAGCCGCAGCC GCCGCCGGCCAGATCCATCATGTTACACAAAATGGAGGACTATATAAAAGACCGTTTAAT GAAGCTTTTGAAGAAACACCAATGCTGGTTGCTGTGCTCACGTATGTGGGGTATGGCGTA CTCACCCTCTTTGGATATCTTCGAGATTTCTTGAGGTATTGGAGAATTGAAAAGTGTCAC CATGCAACAGAAAGAGAAGAACAAAAGGACTTTGTGTCATTGTATCAAGATTTTGAAAAC TTTTATACAAGGAATCTGTACATGAGGATAAGAGACAACTGGAATCGGCCAATCTGTAGT GTGCCTGGAGCCAGGGTGGACATCATGGAGAGACAGTCTCATGATTATAACTGGTCCTTC AAGTATACAGGGAATATAATAAAGGGTGTTATAAACATGGGTTCCTACAACTATCTTGGA TTTGCACGGAATACTGGATCATGTCAAGAAGCAGCCGCCAAAGTCCTTGAGGAGTATGGA GCTGGAGTGTGCAGTACTCGGCAGGAAATTGGAAACCTGGACAAGCATGAAGAACTAGAG GAGCTTGTAGCAAGGTTCTTAGGAGTAGAAGCTGCTATGGCGTATGGCATGGGATTTGCA ACGAATTCAATGAACATTCCTGCTCTTGTTGGCAAAGGTTGCCTGATTCTGAGTGATGAA CTGAACCATGCATCACTGGTTCTGGGAGCCAGACTGTCAGGAGCAACCATTAGAATCTTC AAACACAACAATATGCAAAGCCTAGAGAAGCTATTGAAAGATGCCATTGTTTATGGTCAG CCTCGGACACGAAGGCCCTGGAAGAAAATTCTCATCCTTGTGGAAGGAATATATAGCATG GAGGGATCTATTGTTCGTCTTCCTGAAGTGATTGCCCTCAAGAAGAAATACAAGGCATAC TTGTATCTGGATGAGGCTCACAGCATTGGCGCCCTGGGCCCCACAGGCCGGGGTGTGGTG GAGTACTTTGGCCTGGATCCCGAGGATGTGGATGTTATGATGGGAACGTTCACAAAGAGT TTTGGTGCTTCTGGAGGATATATTGGAGGCAAGAAGGAGCTGATAGACTACCTGCGAACA CATTCTCATAGTGCAGTGTATGCCACGTCATTGTCACCTCCTGTAGTGGAGCAGATCATC ACCTCCATGAAGTGCATCATGGGGCAGGATGGCACCAGCCTTGGTAAAGAGTGTGTACAA CAGTTAGCTGAAAACACCAGGTATTTCAGGAGACGCCTGAAAGAGATGGGCTTCATCATC TATGGAAATGAAGACTCTCCAGTAGTGCCTTTGATGCTCTACATGCCTGCCAAAATTGGC GCCTTTGGACGGGAGATGCTGAAGCGGAACATCGGTGTCGTTGTGGTTGGATTTCCTGCC ACCCCAATTATTGAGTCCAGAGCCAGGTTTTGCCTGTCAGCAGCTCATACCAAAGAAATA CTTGATACTGCTTTAAAGGAGATAGATGAAGTTGGGGACCTATTGCAGCTGAAGTATTCC CGTCATCGGTTGGTACCTCTACTGGACAGGCCCTTTGACGAGACGACGTATGAAGAAACA GAAGACTGA Clone variation with respect to NM_004863.3 786 t=>c >OriGene 5' read for NM_004863 unedited
GCGGCCGCGAATTCGGCACCAGCCGCTTTTTCCCTTCGCTGCGTGGCCTTGGCCGAGACC GGTCCTCTGCGGAGAGGGCCCCGCCCTCTGTGAAGGCCCGCCCGGGAATTGGCGGCGGCG CTGCAGCCATTTCCGGTTTCGGGGAGGTGGGTGGGGTGCGGAGCGGGACTTGGAGCAGCC GCCGCCGCTGCCACCGCCTACAGAGCCTGCCTTGCGCCTGGTGCTGCCAGGAAGATGCGG CCGGAGCCCGGAGGCTGCTGCTGCCGCCGCACGGTGCGGGCGAATGGCTGCGTGGCGAAC GGGGAAGTCCGGAACGGGTACGTGAGGGAGCAGCGCTGCAGCCGCAGCCGCAGCCGCCGC CGGCCAGATCCATTCATGTTACACAAAATGGAGGACTATATAAAAGACCCGTTAATGAAA CTTTTGAAAAACACCAATGCTGGTTGCTGTGCTCACGTATGGGGGGTATGGCGTACTCAC CCTCTTTGGATATCTTTGAGATTCCTTGAGGTATTGGAAATTGAAAAGTGTCCCCTGGCA CAAAAGAGAGACCANAGGGCTTTGGGCATTGATCAAGATTTGAAACTTTATACAGGATCT GACTTGAGGTAAAAGACACTGGATCGGCATTTGTAGGGGCCTGGACCAGGGTGGCATATG GAAAGAAGTCTATGATTTACTGGCCTTCG >OriGene 3' read for NM_004863 unedited
TCTTGGCCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTGAGACAGAGTC TCCCTCTGTCGCCAAGGCTGGAGTGCAGTGGTGTGATCTTGGCTCATTGCATCCTCTGCC TCTTGGGTTCAAGCAATTCCTGTGCCTCAGCCTCCTGACGTAGCTGGGATTACAGGCATG TGCCACCATGCCCGGCTAATTTTTGTAATTTTAGTAGAGATGAGGTTTCGCCATGTTGGC CAGACTGTTCTCGAACTCCTGGCCTCAAGCAATCTGCCTGCTTCAGCCTCCCAAAGTGTT GGGATTACAGGCATGAGCCACTGCGCCCAGCCATGGGCACACATTTCATGTGGATAAAGT TGAGGCGAGAAAAAGACATGTTTTTCTCCACATGGAAGGTATTTTTTAAACTGGTTTAAT GTATTAAGATGGCTGCTGGGCACTGAGCATCCACGTGAAGCAAGTAGCAGGGTATCTGTA GGTTCTGGGACTGAAAAAAAAAATCCCTGTAAGTTCTTGGCTTAGTAGCCAAAAAATAAT ATTTGCTTTTTATACCTCCCCATTCACAACCCCTTTGGGCTAAGCTGATTTAAAAAACAT TTTTTTTCTAAATGGGGTCTTGCTGTGTTGCCCAGGCTACGCCTCAATTCCTGGGGTTAA GCAATCCTCCGCCCTCAGCCTTTCAGTAACTGGGATTGCAAGCTGCATCCGCACCCAGCT CCTAACAATTTTTTTTCCCCCTCTTATTGAACCAGGGGCTCACTTTTTCCACCAAGCCTG AAAGGGCAGGGGTGCAATAATTGGTTCACTGCAACCTTTAACTTCCAAGGTTAAAGGGAA TTCTCCACACCGGGTGGGGAAATACCGCAGGGCCCCACCATGGCCGGGTAATTTAAAAAA AAAATTTTTTAAAAAACAGTGCTATTCTTCCCCGGGTGGCCTAACATTCATAAACTCACG AG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004863 |
| Insert Size | 7180 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004863.2, NP_004854.1 |
| RefSeq Size | 7250 bp |
| RefSeq ORF | 1689 bp |
| Locus ID | 9517 |
| UniProt ID | O15270 |
| Domains | aminotran_1_2 |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | This gene encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. [provided by RefSeq, Mar 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Expression of the ORMDLS, Modulators of Serine Palmitoyltransferase, is Regulated by Sphingolipids in Mammalian Cells
,Gupta, SD;Gable, K;Alexaki, A;Chandris, P;Proia, RL;Dunn, TM;Harmon, JM;,
J. Biol. Chem.
,PubMed ID 25395622
[SPTLC2]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202420 | SPTLC2 (Myc-DDK-tagged)-Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2) |
CNY 4,184.00 |
|
| RC202420L1 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202420L2 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), mGFP tagged |
CNY 6,460.00 |
|
| RC202420L3 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC202420L4 | Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2), mGFP tagged |
CNY 6,460.00 |
|
| RG202420 | SPTLC2 (tGFP-tagged) - Human serine palmitoyltransferase, long chain base subunit 2 (SPTLC2) |
CNY 5,784.00 |
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