Acetyl CoA synthetase (ACSS2) (NM_139274) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACS, ACSA, ACAS2, AceCS, MYH7B, dJ1161H23.1, DKFZp762G026
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_139274, the custom clone sequence may differ by one or more nucleotides ATGAAAGGAGCAACTACCAACATCTGCTACAATGTACTGGATCGAAATGTCCATGAGAAAAAGCTTGGAG ATAAAGTTGCTTTTTACTGGGAGGGCAATGAGCCAGGGGAGACCACTCAGATCACATACCATCAGCTTCT GGTCCAAGTGTGTCAGTTCAGCAATGTTCTCCGAAAACAGGGCATTCAGAAGGGGGACCGAGTGGCCATC TACATGCCTATGATCCCAGAGCTTGTGGTGGCCATGCTGGCATGTGCCCGCATTGGGGCTTTGCACTCCA TTGTGTTTGCAGGCTTCTCTTCAGAGTCTCTATGTGAACGGATCTTGGATTCCAGCTGCAGTCTTCTCAT CACTACAGATGCCTTCTACAGGGGGGAAAAGCTTGTGAACCTGAAGGAGCTGGCTGACGAGGCCCTGCAG AAGTGTCAGGAGAAGGGTTTCCCAGTAAGATGCTGCATTGTGGTCAAGCACCTGGGGCGGGCAGAGCTCG GCATGGGTGACTCCACCAGCCAGTCCCCCCCAATTAAGAGGTCATGCCCAGATGTGCAGATCTCATGGAA CCAAGGGATTGACTTGTGGTGGCATGAGCTCATGCAAGAGGCAGGGGATGAGTGTGAGCCCGAGTGGTGT GATGCCGAGGACCCACTCTTCATCCTGTACACCAGTGGCTCCACAGGCAAACCCAAGGGTGTGGTTCACA CAGTTGGGGGCTACATGCTCTATGTAGCCACAACCTTCAAGTATGTGTTTGACTTCCATGCAGAGGATGT GTTCTGGTGCACGGCAGACATTGGTTGGATCACTGGTCATTCCTACGTCACCTATGGGCCACTGGCCAAT GGTGCCACCAGTGTTTTGTTTGAGGGGATTCCCACATATCCGGACGTGAACCGCCTGTGGAGCATTGTGG ACAAATACAAGGTGACCAAGTTCTACACAGCACCCACAGCCATCCGTCTGCTCATGAAGTTTGGAGATGA GCCTGTCACCAAGCATAGCCGGGCATCCTTGCAGGTGTTAGGCACAGTGGGTGAACCCATCAACCCTGAG GCCTGGCTATGGTACCACCGGGTGGTAGGTGCCCAGCGCTGCCCCATCGTGGACACCTTCTGGCAAACAG AGACAGGTGGCCACATGTTGACTCCCCTTCCTGGTGCCACACCCATGAAACCCGGTTCTGCTACTTTCCC ATTCTTTGGTGTAGCTCCTGCAATCCTGAATGAGTCCGGGGAAGAGTTGGAAGGTGAAGCTGAAGGTTAT CTGGTGTTCAAGCAGCCCTGGCCAGGGATCATGCGCACAGTCTATGGGAACCACGAACGCTTTGAGACAA CCTACTTTAAGAAGTTTCCTGGATACTATGTTACAGGAGATGGCTGCCAGCGGGACCAGGATGGCTATTA CTGGATCACTGGCAGGATTGATGACATGCTCAATGTATCTGGACACCTGCTGAGTACAGCAGAGGTGGAG TCAGCACTTGTGGAACATGAGGCTGTTGCAGAGGCAGCTGTGGTGGGCCACCCTCATCCTGTGAAGGGTG AATGCCTCTACTGCTTTGTCACCTTGTGTGATGGCCACACCTTCAGCCCCAAGCTCACCGAGGAGCTCAA GAAGCAGATTAGAGAAAAGATTGGCCCCATTGCCACACCAGACTACATCCAGAATGCACCTGGCTTGCCT AAAACCCGCTCAGGGAAAATCATGAGGCGAGTGCTTCGGAAGATTGCTCAGAATGACCATGACCTCGGGG ACATGTCTACTGTGGCTGACCCATCTGTCATCAGTCACCTCTTCAGCCACCGCTGCCTGACCATCCAGTG A 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_139274 unedited TCCGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCGGTTCTAGGAACTTGA CGTGATGGGGCTTCCTGAGGAGCGGGTCCGGAGCGGCAGCGGGAGCCGGGGCCAGGAGGA AGCTGGAGCCGGAGGCCGGGCGCGGAGTTGGTCTCCGCCGCCCGAGGTCAGCCGCTCCGC GCACGTCCCCTCGCTGCAGCGCTACCGCGAGCTGCACCGGCGCTCCGTGGAGGAGCCGCG GGAATTCTGGGGAGACATTGCCAAGGAATTTTACTGGAAGACTCCATGCCCTGGCCCATT CCTTCGGTACAACTTTGATGTGACTAAAGGGAAAATCTTTATTGAGTGGATGAAAGGAGC AACTACCAACATCTGCTACAATGTACTGGATCGAAATGTCCATGAGAAAAAGCTTGGAGA TAAAGTTGCTTTTTACTGGGAGGGCAATGAGCCAGGGGAGACCACTCAGATCACATACCA TCAGCTTCTGGTCCAAGTGTGTCAGTTCAGCAATGTTCTCCGAANACAGGGCATTCAGAA GGGGGACCGAGTGGCCATCTACATGCCTATGATCCCAGAGCTTGTGGTGGCCATGCTGGC ATGTGCCCGCATTGGGGCTNTGCACTCCATTGTGTTTGCAGGCTTCTCTTCAGAGTCTCT ATGTGAACGGATCTTGGATTCAGCTGCAGTCTTCTCATCACTACAGATGCCTTCTACAGG NGGGAAAAGCTTGTGAACCTGAAGGAGCTGGCTGACGAGCCCTGCAAAGTGTCANGAGAA TGGTTTCCAGTAAGAGCTGCATTGTGTCAAGCACCTGGGGCGGCANAGCTCGCATGGTGA CTCACCAGCCAGCCCCCCAATAAGAGTCTGCCCAGAGTGCAGACTCATGGACCAGGATTG ACTGGGNGGCATGACTATGCANAGCAGGCAGATGGAACCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_139274 unedited TTTGAACCGTGTGCCGCAATCTACAGTCGAGTTTTTTTTTTTTTTTTTTGAGATCCAGGC TTTATTCCTACAACCACAGGGCTTGAGCCTGACTGGGGCAAGAAACAGAGTTTCATCTGA GAATGTCTCTTATGGGCTGGGTTCTGTTCAGGGGAGGGTGGGAACAGAGGACAAGGAAGA CAAGCTCCTCTGGCCCTAGGAACAAAACACATTTACTCCTTCAAAGAAGCAGATGATCTG AATACCCTCTGGAGACTGAATCTGCCCATACAGCCCCTGGAGCCAATGGGCAGACAGTAC TGGCATCTGGCACAAAAGGGAATTCAGACCCAGAACAGAAGCAGCAAAATATTTTAAAAA TAGCAAATTGTTCCTGGACTCACAAATCATTGTTTTTAAGGGCAAGTGCATGCCCAATAT AAGTACTGGGGCTCCCTAACAGAGCTGACCTAGGATTACACAGCTGCCTCCCTGCTTCAG TGGAGGACCTAACATCCCCTTTGAACACTTAACTTGGGCAGGAGAGGTACCCTTTCCCAC TCTGTTCTGGGCTCTGAGAGCTCTGCAGTCTGGAGGCACAGCACACTGACGCTGACCTGC GCCCTGCCCTTTATGCCTGCCGCCCCAGGATGTTGCCTTTTTCTGGAGACAAAACTGGCT CTCGGTCCCCCACAGTTGGTCAAGGGAGGGAACTGGGCGCCACCACTGTCCCCCACAACA CCTGAGGGGCCCAAAGGATGGGTTCCTCTTTGCCCCACGAGGAACCTCCCGCCACGGTCC CGATCCTGCCTCTCGCATGGCCCGCCCCCGATGGTTGATCAGTGACTGGTGTCCCAGGGC TTTCCCCCCCCACTTGCCCCCGGCCCCGCCCTTCTACCCACTCTTCCCACCTCCCCTCCT GTTTCCCACTCGCCGTTACCCGTTATCTCCCCGCCTTCGCCGCCCCCCCCTCTCCCTTTC CT
Restriction Sites	NotI-NotI
ACCN	NM_139274
Insert Size	3160 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_139274.1, NP_644803.1
RefSeq Size	2773 bp
RefSeq ORF	1821 bp
Locus ID	55902
Protein Pathways	Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
Gene Summary	This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009] Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame segment of the 5' coding region, compared to variant 1. The resulting isoform (b) has a shorter N-terminus when compared to isoform a.

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