ATP5F1B (NM_001686) Human Untagged Clone
CAT#: SC127589
ATP5B (untagged)-Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein
CNY 5,920.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ATP5B; ATPMB; ATPSB; HEL-S-271 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_001686, the custom clone sequence may differ by one or more nucleotides
ATGTTGGGGTTTGTGGGTCGGGTGGCCGCTGCTCCGGCCTCCGGGGCCTTGCGGAGACTCACCCCTTCAG CGTCGCTGCCCCCAGCTCAGCTCTTACTGCGGGCCGCTCCGACGGCGGTCCATCCTGTCAGGGACTATGC GGCGCAAACATCTCCTTCGCCAAAAGCAGGCGCCGCCACCGGGCGCATCGTGGCGGTCATTGGCGCAGTG GTGGACGTCCAGTTTGATGAGGGACTACCACCAATTCTAAATGCCCTGGAAGTGCAAGGCAGGGAGACCA GACTGGTTTTGGAGGTGGCCCAGCATTTGGGTGAGAGCACAGTAAGGACTATTGCTATGGATGGTACAGA AGGCTTGGTTAGAGGCCAGAAAGTACTGGATTCTGGTGCACCAATCAAAATTCCTGTTGGTCCTGAGACT TTGGGCAGAATCATGAATGTCATTGGAGAACCTATTGATGAAAGAGGTCCCATCAAAACCAAACAATTTG CTCCCATTCATGCTGAGGCTCCAGAGTTCATGGAAATGAGTGTTGAGCAGGAAATTCTGGTGACTGGTAT CAAGGTTGTCGATCTGCTAGCTCCCTATGCCAAGGGTGGCAAAATTGGGCTTTTTGGTGGTGCTGGAGTT GGCAAGACTGTACTGATCATGGAGTTAATCAACAATGTCGCCAAAGCCCATGGTGGTTACTCTGTGTTTG CTGGTGTTGGTGAGAGGACCCGTGAAGGCAATGATTTATACCATGAAATGATTGAATCTGGTGTTATCAA CTTAAAAGATGCCACCTCTAAGGTAGCGCTGGTATATGGTCAAATGAATGAACCACCTGGTGCTCGTGCC CGGGTAGCTCTGACTGGGCTGACTGTGGCTGAATACTTCAGAGACCAAGAAGGTCAAGATGTACTGCTAT TTATTGATAACATCTTTCGCTTCACCCAGGCTGGTTCAGAGGTGTCTGCATTATTGGGCCGAATCCCTTC TGCTGTGGGCTATCAGCCTACCCTGGCCACTGACATGGGTACTATGCAGGAAAGAATTACCACTACCAAG AAGGGATCTATCACCTCTGTACAGGCTATCTATGTGCCTGCTGATGACTTGACTGACCCTGCCCCTGCTA CTACGTTTGCCCATTTGGATGCTACCACTGTACTGTCGCGTGCCATTGCTGAGCTGGGCATCTATCCAGC TGTGGATCCTCTAGACTCCACCTCTCGTATCATGGATCCCAACATTGTTGGCAGTGAGCATTACGATGTT GCCCGTGGGGTGCAAAAGATCCTGCAGGACTACAAATCCCTCCAGGATATCATTGCCATCCTGGGTATGG ATGAACTTTCTGAGGAAGACAAGTTGACCGTGTCCCGTGCACGGAAAATACAGCGTTTCTTGTCTCAGCC ATTCCAGGTTGCTGAGGTCTTCACAGGTCATATGGGGAAGCTGGTACCCCTGAAGGAGACCATCAAAGGA TTCCAGCAGATTTTGGCAGGTGAATATGACCATCTCCCAGAACAGGCCTTCTATATGGTGGGACCCATTG AAGAAGCTGTGGCAAAAGCTGATAAGCTGGCTGAAGAGCATTCATCGTGA >OriGene 5' read for NM_001686 unedited
CGCGAATTCGGCACCAGCTACGCCATGTTGGGGTTTGTGGGTCGGGTGGCCGCTGCTCCG GCCTCCGGGGCCTTGCGGAGACTCACCCCTTCAGCGTCGCTGCCCCCAGCTCAGCTCTTA CTGCGGGCCGCTCCGACGGCGGTCCATCCTGTCAGGGACTATGCGGCGCAAACATCTCCT TCGCCAAAAGCAGGCGCCGCCACCGGGCGCATCGTGGCGGTCATTGGCGCAGTGGTGGAC GTCCAGTTTGATGAGGGACTACCACCAATTCTAAATGCCCTGGAAGTGCAAGGCAGGGAG ACCAGACTGGTTTTGGAGGTGGCCCAGCATTTGGGTGAGAGCACAGTAAGGACTATTGCT ATGGATGGTACAGAAGGCTTGGTTAGAGGCCAGAAAGTACTGGATTCTGGTGCACCAATC AAAATTCCTGTTGGTCCTGAGACTTTGGGCAGAATCATGAATGTCATTGGAGAACCTATT GATGAAAGAGGTCCCATCAAAACCAAACAATTTGCTCCCATTCATGCTGAGGCTCCAGAG TTCATGGAAATGAGTGTTGAGCAGGAAATTCTGGTGACTGGTATCAAGGTTGTCGATCTG CTAGCTCCCTATGCCAAGGGTGGNCAAATTGGGCTNTTTGGTGGTGCTGGAGTTGGCAAG ACTGTACTGATCATGGAGTTAATCAAACATGTCGCCAAAGCCCATGGTGGTTACTCTGTG TTTGCTGGTNGTNGGTGAAAGGACCCGTGAN >OriGene 3' read for NM_001686 unedited
ATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGGGTGTACATT TTATTGGAAACCTTAAATACTGTTCAGAAAGAATATATCTTCAATCAAGGCTCTTGTGCA GCCTACACAGAAAAATGAAGCTTTTTGGGTTAGGGGCAAGGAGAGAGACAGTACAGAGGA CAAAGACCCCTCACGATGAATGCTCTTCAGCCAGCTTATCAGCTTTTGCCACAGCTTCTT CAATGGGTCCCACCATATAGAAGGCCTGTTCTGGGAGATGGTCATATTCACCTGCCAAAA TCTGCTGGAATCCTTTGATGGTCTCCTTCAGGGGTACCAGCTTCCCCATATGACCTGTGA AGACCTCAGCAACCTGGAATGGCTGAGACAAGAAACGCTGTATTTTCCGTGCACGGGACA CGGTCAACTTGTCTTCCTCAGAAAGTTCATCCATACCCAGGATGGCAATGATATCCTGGA GGGATTTGTAGTCCTGCAGGATCTTTTGCACCCCACGGGCAACATCGTAATGCTCACTGC CAACAATGTTGGGATCCATGATACGAGAGGTGGAGTCTAGAGGATCCACAGCTGGATAGA TGCCCAGCTCAGCAATGGCACGCGACAGTACAGTGGTAGCATCCAAATGGGCAAACGTAG TAGCAGGGGCAGGGTCAGTCAAGTCATCAGCAGGCACATAGATAGCCTGTACAGAGGTGA TAGATCCCTTCTTGGTAGTGGTAATTCTTTCCTGCATAGTCCCCATGTCAGTGGCCAGGG TAAGCTGATACCCCCCACATAAGGAATTCGGCCCATAATGCANACACCTCTGAACCAGCC TGGGTGAACCGCAGCAGCCTTTATTTAACCCCCCCCTTCTCGACCCTTTTGGTCTTGAAG TTTTCACCCCATCAGCCCATACAGATTACCGGCAACAGCCCAGGGGTTATTAATTGACCC CACAACGCTCCCTCAAGGGCATTTCTAAGTGAAACCCA |
Restriction Sites | NotI-NotI |
ACCN | NM_001686 |
Insert Size | 1750 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001686.3, NP_001677.2 |
RefSeq Size | 1857 bp |
RefSeq ORF | 1590 bp |
Locus ID | 506 |
UniProt ID | P06576 |
Domains | ATP-synt_ab, ATP-synt_ab_C, AAA, ATP-synt_ab_N |
Protein Families | Druggable Genome |
Protein Pathways | Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease |
Gene Summary | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008] |
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Resources
Other Versions
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RC201638 | ATP5B (Myc-DDK-tagged)-Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein |
CNY 5,904.00 |
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RC201638L1 | Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,304.00 |
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RC201638L2 | Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
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RC201638L3 | Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC201638L4 | Lenti ORF clone of Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,304.00 |
|
RG201638 | ATP5B (tGFP-tagged) - Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein |
CNY 7,504.00 |
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SC322509 | ATP5B (untagged)-Human ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), nuclear gene encoding mitochondrial protein |
CNY 5,920.00 |
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