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EGF (NM_001963) Human Untagged Clone
CAT＃: SC127840
EGF (untagged)-Human epidermal growth factor (EGF), transcript variant 1
CNY 8,408.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HOMG4; URG |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC127840 sequence for NM_001963 edited (data generated by NextGen Sequencing)
ATGCTGCTCACTCTTATCATTCTGTTGCCAGTAGTTTCAAAATTTAGTTTTGTTAGTCTC TCAGCACCGCAGCACTGGAGCTGTCCTGAAGGTACTCTCGCAGGAAATGGGAATTCTACT TGTGTGGGTCCTGCACCCTTCTTAATTTTCTCCCATGGAAATAGTATCTTTAGGATTGAC ACAGAAGGAACCAATTATGAGCAATTGGTGGTGGATGCTGGTGTCTCAGTGATCATGGAT TTTCATTATAATGAGAAAAGAATCTATTGGGTGGATTTAGAAAGACAACTTTTGCAAAGA GTTTTTCTGAATGGGTCAAGGCAAGAGAGAGTATGTAATATAGAGAAAAATGTTTCTGGA ATGGCAATAAATTGGATAAATGAAGAAGTTATTTGGTCAAATCAACAGGAAGGAATCATT ACAGTAACAGATATGAAAGGAAATAATTCCCACATTCTTTTAAGTGCTTTAAAATATCCT GCAAATGTAGCAGTTGATCCAGTAGAAAGGTTTATATTTTGGTCTTCAGAGGTGGCTGGA AGCCTTTATAGAGCAGATCTCGATGGTGTGGGAGTGAAGGCTCTGTTGGAGACATCAGAG AAAATAACAGCTGTGTCATTGGATGTGCTTGATAAGCGGCTGTTTTGGATTCAGTACAAC AGAGAAGGAAGCAATTCTCTTATTTGCTCCTGTGATTATGATGGAGGTTCTGTCCACATT AGTAAACATCCAACACAGCATAATTTGTTTGCAATGTCCCTTTTTGGTGACCGTATCTTC TATTCAACATGGAAAATGAAGACAATTTGGATAGCCAACAAACACACTGGAAAGGACATG GTTAGAATTAACCTCCATTCATCATTTGTACCACTTGGTGAACTGAAAGTAGTGCATCCA CTTGCACAACCCAAGGCAGAAGATGACACTTGGGAGCCTGAGCAGAAACTTTGCAAATTG AGGAAAGGAAACTGCAGCAGCACTGTGTGTGGGCAAGACCTCCAGTCACACTTGTGCATG TGTGCAGAGGGATACGCCCTAAGTCGAGACCGGAAGTACTGTGAAGATGTTAATGAATGT GCTTTTTGGAATCATGGCTGTACTCTTGGGTGTAAAAACACCCCTGGATCCTATTACTGC ACGTGCCCTGTAGGATTTGTTCTGCTTCCTGATGGGAAACGATGTCATCAACTTGTTTCC TGTCCACGCAATGTGTCTGAATGCAGCCATGACTGTGTTCTGACATCAGAAGGTCCCTTA TGTTTCTGTCCTGAAGGCTCAGTGCTTGAGAAAGATGGGAAAACATGTAGCGGTTGTTCC TCACCCGATAATGGTGGATGTAGCCAGCTCTGCGTTCCTCTTAGCCCAGTATCCTGGGAA TGTGATTGCTTTCCTGGGTATGACCTACAACTGGATGAAAAAAGCTGTGCAGCTTCAGGA CCACAACCATTTTTGCTGTTTGCCAATTCTCAAGATATTCGACACATGCATTTTGATGGA ACAGACTATGGAACTCTGCTCAGCCAGCAGATGGGAATGGTTTATGCCCTAGATCATGAC CCTGTGGAAAATAAGATATACTTTGCCCATACAGCCCTGAAGTGGATAGAGAGAGCTAAT ATGGATGGTTCCCAGCGAGAAAGGCTTATTGAGGAAGGAGTAGATGTGCCAGAAGGTCTT GCTGTGGACTGGATTGGCCGTAGATTCTATTGGACAGACAGAGGGAAATCTCTGATTGGA AGGAGTGATTTAAATGGGAAACGTTCCAAAATAATCACTAAGGAGAACATCTCTCAACCA CGAGGAATTGCTGTTCATCCAATGGCCAAGAGATTATTCTGGACTGATACAGGGATTAAT CCACGAATTGAAAGTTCTTCCCTCCAAGGCCTTGGCCGTCTGGTTATAGCCAGCTCTGAT CTAATCTGGCCCAGTGGAATAACGATTGACTTCTTAACTGACAAGTTGTACTGGTGCGAT GCCAAGCAGTCTGTGATTGAAATGGCCAATCTGGATGGTTCAAAACGCCGAAGACTTACC CAGAATGATGTAGGTCACCCATTTGCTGTAGCAGTGTTTGAGGATTATGTGTGGTTCTCA GATTGGGCTATGCCATCAGTAATAAGAGTAAACAAGAGGACTGGCAAAGATAGAGTACGT CTCCAAGGCAGCATGCTGAAGCCCTCATCACTGGTTGTGGTTCATCCATTGGCAAAACCA GGAGCAGATCCCTGCTTATATCAAAACGGAGGCTGTGAACATATTTGCAAAAAGAGGCTT GGAACTGCTTGGTGTTCGTGTCGTGAAGGTTTTATGAAAGCCTCAGATGGGAAAACGTGT CTGGCTCTGGTTGGTCATCAGCTGTTGGCAGGTGGTGAAGTTGATCTAAAGAACCAAGTA ACACCATTGGACATCTTGTCCAAGACTAGAGTGTCAGAAGATAACATTACAGAATCTCAA CACATGCTAGTGGCTGAAATCATGGTGTCAGATCAAGATGACTGTGCTCCTGTGGGATGC AGCATGTATGCTCGGTGTATTTCAGAGGGAGAGGATGCCACATGTCAGTGTTTGAAAGGA TTTGCTGGGGATGGAAAACTATGTTCTGATATAGATGAATGTGAGATGGGTGTCCCAGTG TGCCCCCCTGCCTCCTCCAAGTGCATCAACACCGAAGGTGGTTATGTCTGCCGGTGCTCA GAAGGCTACCAAGGAGATGGGATTCACTGTCTTGATATTGATGAGTGCCAACTGGGGGTG CACAGCTGTGGAGAGAATGCCAGCTGCACAAATACAGAGGGAGGCTATACCTGCATGTGT GCTGGACGCCTGTCTGAACCAGGACTGATTTGCCCTGACTCTACTCCACCCCCTCACCTC AGGGAAGATGACCACCACTATTCCGTAAGAAATAGTGACTCTGAATGTCCCCTGTCCCAC GATGGGTACTGCCTCCATGATGGTGTGTGCATGTATATTGAAGCATTGGACAAGTATGCA TGCAACTGTGTTGTTGGCTACATCGGGGAGCGATGTCAGTACCGAGACCTGAAGTGGTGG GAACTGCGCCACGCTGGCCACGGGCAGCAGCAGAAGGTCATCGTGGTGGCTGTCTGCGTG GTGGTGCTTGTCATGCTGCTCCTCCTGAGCCTGTGGGGGGCCCACTACTACAGGACTCAG AAGCTGCTATCGAAAAACCCAAAGAATCCTTATGAGGAGTCGAGCAGAGATGTGAGGAGT CGCAGGCCTGCTGACACTGAGGATGGGATGTCCTCTTGCCCTCAACCTTGGTTTGTGGTT ATAAAAGAACACCAAGACCTCAAGAATGGGGGTCAACCAGTGGCTGGTGAGGATGGCCAG GCAGCAGATGGGTCAATGCAACCAACTTCATGGAGGCAGGAGCCCCAGTTATGTGGAATG GGCACAGAGCAAGGCTGCTGGATTCCAGTATCCAGTGATAAGGGCTCCTGTCCCCAGGTA ATGGAGCGAAGCTTTCATATGCCCTCCTATGGGACACAGACCCTTGAAGGGGGTGTCGAG AAGCCCCATTCTCTCCTATCAGCTAACCCATTATGGCAACAAAGGGCCCTGGACCCACCA CACCAAATGGAGCTGACTCAGTGA Clone variation with respect to NM_001963.4 1292 g=>a;2124 g=>a;2351 a=>t;2759 a=>t >OriGene 5' read for NM_001963 unedited
TTCTGACGAATCGCCTACGCCCGNCGTCGAGGGGNCGGTTGGAGTGCACGGTGGGAGGTC TATATAAGCTGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGG CGGCCGCGAATTCGGCACGAGAGGAATCGTATCTCCATATTTCTTCTTTCAGCCCCAATC CAAGGGTTGTGGCTGGAACTTTCCATCAGTTCTTCCTTTCTTTTTCCTCTCTAAGCCTTT GCCTTGCTCTGTCACAGTGAAGTCAGCCAGAGCAGGGCTGTTAAACTCTGTGAAATTTGT CATAAGGGTGTCAGGTATTTCTTACTGGCTTCCAAAGAAACATAGATAAAGAAATCTTTC CTGTGGCTTCCCTTGGCAGGCTGCATTCAGAAGGTCTCTCAGTTGAAGAAAGAGCTTGGA GGACAACAGCACAACAGGAGAGTAAAAGATGCCCCAGGGCTGAGGCCTCCGCTCAGGCAG CCGCATCTGGGGTCAATCATACTCACCTTGCCCGGGCCATGCTCCAGCAAAATCAAGCTG TTTTCTTTTGAAAGTTCAAACTCATCAAGATTATGCTGCTCACTCTTATCATTCTGTTGC CAGTAGTTTCAAAATTTAGTTTTGTTAGTCCTCTCAGCACCGCAGCACTTGGAGCTGTCC TGAAGGTACTCTCCGCAGAAATGGGAATTCTACTTGTGTGGGTCCTGGCCCCTTCCTTAT TTTCTCCCTTGGGAATAGTTTCTTTAGGTTTGCCCCGGAAGGACCCATTATGAACCAATG GTGGTGGATGCTGGGGGCTCAGCGATCACGGATTTTCTTATTATGGGCAAAGAAATTTTG GGTGGGTTGAGAAAGACACTTTTGCCAAAGGTTTTTCTGAATGGTCCAGGCGAGGAAAAG TTTGTCAATTAAAGAAAAATGGTTGGGAAGGGACAAAATAG >OriGene 3' read for NM_001963 unedited
CCAATACTGTGTACGAGCCGCATCCTAGGATCGAGTTAATTCTTTTTTTTTTTTTACTTC AATTTCTTTTTATTCTGATGTCTGGTATCACCAAAGTCAGGGGTTGAGAAATGAAAGATA AACAAAGGGACTAGTTAATGCATAAACTGAATGATTCAAAGCCCCCAAAATTTAAAGTTA CCATATCAGGTTGTGTTATCTGTAGTAACAATATTTTTTCTGCAAGAAAAATATTTAATT AGAACACCATGTGAAATTTTAATCTACAGAGTTGTAGATTAAAAAATTTTTTTAAAAGTT ACAAGGCTGGGCACCGTGGCTAATGCCTATAATCTAATACGTTGGGAGGCTGAAAGTCAA TCACAATTCATTTTGCAAAATTACCAGCACGACCTTGCTTGAGGCCAGGAGTTCAAGACC AGCCTGGGCAACATTGTGTGATCCCCATGTCTACAAAAAATAAAAATAAAAACCTTAGCC AGGCATGGTGGCCTGTGCCTGTGGTCCTAGTTACTCGGGAGGCTGAGGCAGGAGGATTGC TTGAGCCCAGGAGTTTGAGGCTGCACTGAGCTATGATTGTGCCATTGCACACTCCAGCCT GGATAACAGAGTGAGATCCTATCCTCTCTCCGGGAAAAAAAAAAAAACGAAAAACAGAAA CATCAGCAAGCTTGACAAATCATTTTGGTTTCAACTCTAGTACTGCCTTAAACTATAATT AACTACTTTATCCAGCATACAATAAAATGTTTAATGTTTTGGGTGATTTTATTTAAAAAA TTTGAAGAAATTCATCGTTTTTCTATAATGAAAACCAATTCAGGCATATTACTTTCCAAT CTTGTTAACTGAAAAAACATACCAATTTTTGGGCATTCACCCCCAAGAGGGAAAATTTTT GTGGGGAN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001963 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001963.2, NP_001954.1 |
| RefSeq Size | 4877 bp |
| RefSeq ORF | 3624 bp |
| Locus ID | 1950 |
| UniProt ID | P01133 |
| Domains | ldl_recept_b, EGF_CA, EGF, EGF |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane |
| Protein Pathways | Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Autocrine EGF and TGF ‐α promote primary and acquired resistance to ALK / c‐Met kinase inhibitors in non‐small‐cell lung cancer
,null,
Pharmacology Research & Perspectives
,PubMed ID 36583451
[EGF]
|
|
EGFR ligands exhibit functional differences in models of paracrine and autocrine signaling
,null,
Growth factors (Chur, Switzerland)
,PubMed ID 22260327
[EGF]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210817 | EGF (Myc-DDK-tagged)-Human epidermal growth factor (EGF), transcript variant 1 |
CNY 8,408.00 |
|
| RC210817L1 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, Myc-DDK-tagged |
CNY 10,808.00 |
|
| RC210817L2 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, mGFP tagged |
CNY 13,970.00 |
|
| RC210817L3 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, Myc-DDK-tagged |
CNY 10,808.00 |
|
| RC210817L4 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, mGFP tagged |
CNY 10,808.00 |
|
| RG210817 | EGF (tGFP-tagged) - Human epidermal growth factor (EGF), transcript variant 1 |
CNY 13,300.00 |
