ASAP1 (NM_018482) Human Untagged Clone
CAT#: SC127851
ASAP1 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1)
CNY 7,872.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | AMAP1; CENTB4; DDEF1; PAG2; PAP; ZG14P |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_018482, the custom clone sequence may differ by one or more nucleotides
ATGAGATCTTCAGCCTCCAGGCTCTCCAGTTTTTCGTCGAGAGATTCACTATGGAATCGGATGCCGGACC AGATCTCTGTCTCGGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCAC CACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCC CTTCAGAAAGTGAAGAAGTCTGTAAAAGCAATATATAATTCTGGTCAAGATCATGTACAAAATGAAGAAA ACTATGCACAAGTTCTTGATAAGTTTGGGAGTAATTTTTTAAGTCGAGACAACCCCGACCTTGGCACCGC GTTTGTCAAGTTTTCTACTCTTACAAAGGAACTGTCCACACTGCTGAAAAATCTGCTCCAGGGTTTGAGC CACAATGTGATCTTCACCTTGGATTCTTTGTTAAAAGGAGACCTAAAGGGAGTCAAAGGAGATCTCAAGA AGCCATTTGACAAAGCCTGGAAAGATTATGAGACAAAGTTTACAAAAATTGAGAAAGAGAAAAGAGAGCA CGCAAAACAACATGGGATGATCCGCACAGAGATAACAGGAGCTGAGATTGCGGAAGAAATGGAGAAGGAA AGGCGCCTCTTTCAGCTCCAAATGTGTGAATATCTCATTAAAGTTAATGAAATCAAGACCAAAAAGGGTG TGGATCTGCTGCAGAATCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAAGATGGCTTGAAAAC AGCTGATAAGTTGAAACAGTACATTGAAAAACTGGCTGCTGATTTATATAATATAAAACAGACCCAGGAT GAAGAAAAGAAACAGCTAACTGCACTCCGAGACTTAATAAAATCCTCTCTTCAACTGGATCAGAAAGAAG ATTCTCAGAGCCGGCAAGGAGGATACAGCATGCATCAGCTCCAGGGCAATAAGGAATATGGCAGTGAAAA GAAGGGGTACCTGCTAAAGAAAAGTGACGGGATCCGGAAAGTATGGCAGAGGAGGAAGTGTTCAGTCAAG AATGGGATTCTGACCATCTCACATGCCACATCTAACAGGCAACCAGCCAAGTTGAACCTTCTCACCTGCC AAGTAAAACCTAATGCCGAAGACAAAAAATCTTTTGACCTGATATCACATAATAGAACATATCACTTTCA GGCAGAAGATGAGCAGGATTATGTAGCATGGATATCAGTATTGACAAATAGCAAAGAAGAGGCCCTAACC ATGGCCTTCCGTGGAGAGCAGAGTGCGGGAGAGAACAGCCTGGAAGACCTGACAAAAGCCATTATTGAGG ATGTCCAGCGGCTCCCAGGGAATGACATTTGCTGCGATTGTGGCTCATCAGAACCCACCTGGCTTTCAAC CAACTTGGGTATTTTGACCTGTATAGAATGTTCTGGCATCCATAGGGAAATGGGGGTTCATATTTCTCGC ATTCAGTCTTTGGAACTAGACAAATTAGGAACTTCTGAACTCTTGCTGGCCAAGAATGTAGGAAACAATA GTTTTAATGATATTATGGAAGCAAATTTACCCAGCCCCTCACCAAAACCCACCCCTTCAAGTGATATGAC TGTACGAAAAGAATATATCACTGCAAAGTATGTAGATCATAGGTTTTCAAGGAAGACCTGTTCAACTTCA TCAGCTAAACTAAATGAATTGCTTGAGGCCATCAAATCCAGGGATTTACTTGCACTAATTCAAGTCTATG CAGAAGGGGTAGAGCTAATGGAACCACTGCTGGAACCTGGGCAGGAGCTTGGGGAGACAGCCCTTCACCT TGCCGTCCGAACTGCAGATCAGACATCTCTCCATTTGGTTGACTTCCTTGTACAAAACTGTGGGAACCTG GATAAGCAGACGGCCCTGGGAAACACAGTTCTACACTACTGTAGTATGTACAGTAAACCTGAGTGTTTGA AGCTTTTGCTCAGGAGCAAGCCCACTGTGGATATAGTTAACCAGGCTGGAGAAACTGCCCTAGACATAGC AAAGAGACTAAAAGCTACCCAGTGTGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTTCAATCCACAC GTCCACGTAGAATATGAGTGGAATCTTCGACAGGAGGAGATAGATGAGAGCGATGATGATCTGGATGACA AACCAAGCCCTATCAAGAAAGAGCGCTCACCCAGACCTCAGAGCTTCTGCCACTCCTCCAGCATCTCCCC CCAGGACAAGCTGGCACTGCCAGGATTCAGCACTCCAAGGGACAAACAGCGGCTCTCCTATGGAGCCTTC ACCAACCAGATCTTCGTTTCCACAAGCACAGACTCGCCCACATCACCAACCACGGAGGCTCCCCCTCTGC CTCCTAGGAACGCCGGGAAAGGTCCAACTGGCCCACCTTCAACACTCCCTCTAAGCACCCAGACCTCTAG TGGCAGCTCCACCCTATCCAAGAAGAGGCCTCCTCCCCCACCACCCGGACACAAGAGAACCCTATCCGAC CCTCCCAGCCCACTACCTCATGGGCCCCCAAACAAAGGCGCAGTTCCTTGGGGTAACGATGGGGGTCCAT CCTCTTCAAGTAAGACTACAAACAAGTTTGAGGGACTATCCCAGCAGTCGAGCACCAGTTCTGCAAAGAC TGCCCTTGGCCCAAGAGTTCTTCCTAAACTACCTCAGAAAGTGGCACTAAGGAAAACAGATCATCTCTCC CTAGACAAAGCCACCATCCCGCCCGAAATCTTTCAGAAATCATCACAGTTGGCAGAGTTGCCACAAAAGC CACCACCTGGAGACCTGCCCCCAAAGCCCACAGAACTGGCCCCCAAGCCCCAAATTGGAGATTTGCCGCC TAAGCCAGGAGAACTGCCCCCCAAACCACAGCTGGGGGACCTGCCACCCAAACCCCAACTCTCAGACTTA CCTCCCAAACCACAGATGAAGGACCTGCCCCCCAAACCACAGCTGGGAGACCTGCTAGCAAAATCCCAGA CTGGAGATGTCTCACCCAAGGCTCAGCAACCCTCTGAGGTCACACTGAAGTCACACCCATTGGATCTATC CCCAAATGTGCAGTCCAGAGACGCCATCCAAAAGCAAGCATCTGAAGACTCCAACGACCTCACGCCTACT CTGCCAGAGACGCCCGTACCACTGCCCAGAAAAATCAATACGGGGAAAAATAAAGTGAGGCGAGTGAAGA CCATTTATGACTGCCAGGCAGACAACGATGACGAGCTCACATTCATCGAGGGAGAAGTGATTATCGTCAC AGGGGAAGAGGACCAGGAGTGGTGGATTGGCCACATCGAAGGACAGCCTGAAAGGAAGGGGGTCTTTCCA GTGTCCTTTGTTCATATCCTGTCTGACTAG >OriGene 5' read for NM_018482 unedited
AGGGCGGCCCGCGAATTCGGCACGAGGCGCCCCTTCCTGCGCCGCGCTCGGCTCCCGCGC CTCGCCTGGCCTGGCCGCTGCCGCGGGCGAGCACTGTGCGCTGGGCAGCCCCGGGCCCGC GCCTCCCGCCCAGCCGGCGCTTCCCGGTCGCAGTTCGCTTTCCCACCCGAGAGCCGCGAG CGCCCGGCCGAACCCGCGATGGAATAATGCCCAGCGGCCCGCCAGGTCCCGGTCGTTTTC TGATGTGACGGCTGAGACATGAGATCTTCAGCCTCCAGGCTCTCCAGTTTTTCGTCGAGA GATTCACTATGGAATCGGATGCCGGACCAGATCTCTGTCTCGGAGTTCATCGCCGAGACC ACCGAGGACTACAACTCGCCCACCACGTCCAGCTTCACCACGCGGCTGCACAACTGCAGG AACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGATAGAACAGCCCTTCAGAAAGTG AAGAAGTCTGTAAAAGCAATATATAATTCTGGTCAAGATCATGTACAAAATGAAGAAAAC TATGCACAAGTTCTTGATAAGTTTGGGAGTAATTTTCTAAGTCGAGACAACCCCGACCTT GGCACCGCGTTTGTCAAGTTTTCTACTCTTACCAAGGAACTGTCCACACTTGCTGAAAAA ATCTGCTCCCAGGGTTTGAACCACCAATGTGATCTTTCCCCTTGGGATTCTTTTGTTAAA AAGGAAAACCTTAAAAGCGATTCCAAGAGAGATCTTAAAGAATGCCATTTGGACCAAAGC CTGGAAAGACTATGAGACAAAGCTTCCAAAAACGGAGAAAGAAAAAAGAGAGCACGCAAA ACACTTGTGATGACCGGCCAGAGATACCAGATCCGTCATCGCCGTAAAATGAGAAAGAAA GGCGCCTCTTTCAACCCAAAGGGTGAAT >OriGene 3' read for NM_018482 unedited
CATTGAAAAATTATACTCAGGACCACAGTGAAAAAAACAGCATCTGTTAATGCCAAGCTT ATAAATAGTGCTAAATGTAATGAACTCGGGNACACTCGACCAACCTCATATGCAGACTTC CATCCCGGACACTGGTGTGGCTTCAGTACTGAAGCTGCTAAAGCTGCCAATCCCAACCCA TCATGTCAACTGGTTCCTCATAGCTCTGTTTGGTGTGGAAATTCACATGTGCCCTGACAC TGACGAAGCAGTTGCTTAAAATCACTTTCCAATAACATCTGATAAAATATTTTGCAAGTT TGCCATGCAAAGATTATTTATTAAGAGGCTATTCAAAGTTTGTACAACAACCACTTAAAC AGAACTCACTTAATATTCACTAGATGCACTGTATCGATAGGAATAGGGACTTTTCCTAAA GGTTGACTTGGGAATTGTGCTTCTGTGAAATTAACATCTCTCACTGATTGACAAGATTCT ATACTTAAAAATATTAATTCTCTGTGCTGACGCCAATATAGCATTTAAACAGATGCATCC CACATTGATAATTATCGCTGTACTCATGTAAAAATCCCATTGACCGTGAAACTACACCAG TGAATAACTTTCCAAAAATAAGACTGATGTGAAAGACTGACTTCCCAAGTTCTCAAAGTT CCGAAAACCCTGTCCATGTATTACAAGACACTAATATGGAAAAAGTGCTTTTTCTAGTGC AAGCCTTACACGATTCCAAACACATGGCCCCACACTTGAATCTGATTTGAACGGAGTCTG ATATATGGACCAGTTTCCTTAAATTTGCGCCCACACCATTTCGCGCGAAATTTCCCTTGC GCCCTAGTAACAGCCCTTAAATCACGCGACGGACACCGCCACAAGGTTTAAATCCCCCAC TCGATTTGTTATTCCGCCACCCCAATTATTTTTTGTTCCC |
Restriction Sites | NotI-NotI |
ACCN | NM_018482 |
Insert Size | 6000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018482.2, NP_060952.2 |
RefSeq Size | 6042 bp |
RefSeq ORF | 3390 bp |
Locus ID | 50807 |
UniProt ID | Q9ULH1 |
Protein Pathways | Endocytosis, Fc gamma R-mediated phagocytosis |
Gene Summary | This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Identification of a link between the SAMP repeats of APC (adenomatous polyposis coli) tumor suppressor and the SH3 domain of DDEF
,Chiyuki Matsui, Shuji Kaieda, Takahisa Ikegami, and Yuko Mimori-Kiyosue,
J. Biol. Chem., Sep 2008; 10.1074/jbc.M800420200
[ASAP1]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217979 | ASAP1 (Myc-DDK-tagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) |
CNY 7,864.00 |
|
RC217979L1 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), Myc-DDK-tagged |
CNY 10,264.00 |
|
RC217979L2 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mGFP tagged |
CNY 13,210.00 |
|
RC217979L3 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), Myc-DDK-tagged |
CNY 13,210.00 |
|
RC217979L4 | Lenti ORF clone of Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1), mGFP tagged |
CNY 13,210.00 |
|
RG217979 | ASAP1 (tGFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) |
CNY 9,464.00 |