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SIRT1 (NM_012238) Human Untagged Clone
CAT＃: SC127917
SIRT1 (untagged)-Human sirtuin 1 (SIRT1), transcript variant 1
CNY 8,216.00
| Cited in 6 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SIR2; SIR2alpha; SIR2L1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_012238, the custom clone sequence may differ by one or more nucleotides
ATGGCGGACGAGGCGGCCCTCGCCCTTCAGCCCGGCGGCTCCCCCTCGGCGGCGGGGGCCGACAGGGAGG CCGCGTCGTCCCCCGCCGGGGAGCCGCTCCGCAAGAGGCCGCGGAGAGATGGTCCCGGCCTCGAGCGGAG CCCGGGCGAGCCCGGTGGGGCGGCCCCAGAGCGTGAGGTGCCGGCGGCGGCCAGGGGCTGCCCGGGTGCG GCGGCGGCGGCGCTGTGGCGGGAGGCGGAGGCAGAGGCGGCGGCGGCAGGCGGGGAGCAAGAGGCCCAGG CGACTGCGGCGGCTGGGGAAGGAGACAATGGGCCGGGCCTGCAGGGCCCATCTCGGGAGCCACCGCTGGC CGACAACTTGTACGACGAAGACGACGACGACGAGGGCGAGGAGGAGGAAGAGGCGGCGGCGGCGGCGATT GGGTACCGAGATAACCTTCTGTTCGGTGATGAAATTATCACTAATGGTTTTCATTCCTGTGAAAGTGATG AGGAGGATAGAGCCTCACATGCAAGCTCTAGTGACTGGACTCCAAGGCCACGGATAGGTCCATATACTTT TGTTCAGCAACATCTTATGATTGGCACAGATCCTCGAACAATTCTTAAAGATTTATTGCCGGAAACAATA CCTCCACCTGAGTTGGATGATATGACACTGTGGCAGATTGTTATTAATATCCTTTCAGAACCACCAAAAA GGAAAAAAAGAAAAGATATTAATACAATTGAAGATGCTGTGAAATTACTGCAAGAGTGCAAAAAAATTAT AGTTCTAACTGGAGCTGGGGTGTCTGTTTCATGTGGAATACCTGACTTCAGGTCAAGGGATGGTATTTAT GCTCGCCTTGCTGTAGACTTCCCAGATCTTCCAGATCCTCAAGCGATGTTTGATATTGAATATTTCAGAA AAGATCCAAGACCATTCTTCAAGTTTGCAAAGGAAATATATCCTGGACAATTCCAGCCATCTCTCTGTCA CAAATTCATAGCCTTGTCAGATAAGGAAGGAAAACTACTTCGCAACTATACCCAGAACATAGACACGCTG GAACAGGTTGCGGGAATCCAAAGGATAATTCAGTGTCATGGTTCCTTTGCAACAGCATCTTGCCTGATTT GTAAATACAAAGTTGACTGTGAAGCTGTACGAGGAGATATTTTTAATCAGGTAGTTCCTCGATGTCCTAG GTGCCCAGCTGATGAACCGCTTGCTATCATGAAACCAGAGATTGTGTTTTTTGGTGAAAATTTACCAGAA CAGTTTCATAGAGCCATGAAGTATGACAAAGATGAAGTTGACCTCCTCATTGTTATTGGGTCTTCCCTCA AAGTAAGACCAGTAGCACTAATTCCAAGTTCCATACCCCATGAAGTGCCTCAGATATTAATTAATAGAGA ACCTTTGCCTCATCTGCATTTTGATGTAGAGCTTCTTGGAGACTGTGATGTCATAATTAATGAATTGTGT CATAGGTTAGGTGGTGAATATGCCAAACTTTGCTGTAACCCTGTAAAGCTTTCAGAAATTACTGAAAAAC CTCCACGAACACAAAAAGAATTGGCTTATTTGTCAGAGTTGCCACCCACACCTCTTCATGTTTCAGAAGA CTCAAGTTCACCAGAAAGAACTTCACCACCAGATTCTTCAGTGATTGTCACACTTTTAGACCAAGCAGCT AAGAGTAATGATGATTTAGATGTGTCTGAATCAAAAGGTTGTATGGAAGAAAAACCACAGGAAGTACAAA CTTCTAGGAATGTTGAAAGTATTGCTGAACAGATGGAAAATCCGGATTTGAAGAATGTTGGTTCTAGTAC TGGGGAGAAAAATGAAAGAACTTCAGTGGCTGGAACAGTGAGAAAATGCTGGCCTAATAGAGTGGCAAAG GAGCAGATTAGTAGGCGGCTTGATGGTAATCAGTATCTGTTTTTGCCACCAAATCGTTACATTTTCCATG GCGCTGAGGTATATTCAGACTCTGAAGATGACGTCTTATCCTCTAGTTCTTGTGGCAGTAACAGTGATAG TGGGACATGCCAGAGTCCAAGTTTAGAAGAACCCATGGAGGATGAAAGTGAAATTGAAGAATTCTACAAT GGCTTAGAAGATGAGCCTGATGTTCCAGAGAGAGCTGGAGGAGCTGGATTTGGGACTGATGGAGATGATC AAGAGGCAATTAATGAAGCTATATCTGTGAAACAGGAAGTAACAGACATGAACTATCCATCAAACAAATC ATAG >OriGene 5' read for NM_012238 unedited
GACTTCCGCCCGCTTGCAGGGCGGAGCGTAGGGATGGAGGTGGTATAAGCGAGCTCGTGT AGTGATCGTCAGAATTTTGTACCAGGGCAGTTGGAAGATGGCGGACGAGGCGGCCCTCGC CCTTCAGCCCGGCGGCTCCCCCTCGGCGGCGGGGGCCGACAGGGAGGCCGCGTCGTCCCC CGCCGGGGAGCCGCTCCGCAAGAGGCCGCGGAGAGATGGTCCCGGCCTCGAGCGGAGCCC GGGCGAGCCCGGTGGGGCGGCCCCAGAGCGTGAGGTGCCGGCGGCGGCCAGGGGCTGCCC GGGTGCGGCGGCGGCGGCGCTGTGGCGGGAGGCGGAGGCAGAGGCGGCGGCGGCAGGCGG GGAGCAAGAGGCCCAGGCGACTGCGGCGGCTGGGGAAGGAGACAATGGGCCGGGCCTGCA GGGCCCATCTCGGGAGCCACCGCTGGCCGACAACTTGTACGACGAAGACGACGACGACGA GGGCGAGGAGGAGGAAGAGGCGGCGGCGGCGGCGATTGGGTACCGAGATAACCTTCTGTT CGGTGATGAAATTATCACTAATGGTTTTCATTCCTGTGAAAGTGATGAGGAGGATAGAGC CTCACATGCAAGCTCTAGTGACTGGACTCCAAGGCCACGGATAGGTCCATATACTTTTGT TCAGCAACATCTTATGATTGGCACAGATCCTCGAACAATTCTTAAAGATTTATTGCCGGA AACAATACCTCCACCTGAGTTGGATGATATGACACTGTGGCAGATTGTTATTATATCCTT TCAGAACCACCAAAAAGGAAAAAAAGAAAAGATATTAATACAATTGAGATGCTGTGAAAT TACTGCAGGAGTGCAAAAAAACTATAGTTCTAACTGCAGCTGGGGTGTCTGCTTTCATGT AGAATACCTGACTGTCAGTTCAAGGGATGGTATTTTAATGCTCGAC >OriGene 3' read for NM_012238 unedited
GCTACTGCCTACGATACTTGTATTTAAAAGTTGAAGGAAGAAAACCAGTCATTACACACA GTCTACAAAACATATGCCAGTAAATTACATAAAAGACTATGTACAATATAAAAAGAGCTG AAAACAGTCTTCACTGTAAAAATAATTTAAAACAAACTTTTCAATTTAAAATATCATCTA TAGCACACAAACATCATGCAAATGGAAAACTAAATATACTGCATTCTTTAGTGTAGCCAA ATAAATTCAGATTGAGACATCTTATAAGTAGGGAAATGGCCATTCAATACGATTTTTTTC TCTGGCAGTAATGGTCCTAGCTGGGTGTTTTATGCATAAAGAACAGCTATATTTCAAACC CTTTTTATTGTAATAAATACTAAAGCAACAGAGGAATACTTTATTAATTTAGGAGTGATG TTCAAAAATGGTCTGAAAAATAAATGCTTACTGTGCAATTTCATAAAGTATGAACTTCTT GAAAGACTAGAAGCTTTTGCAGCTGAGAAAGTTGATCTCTAGTTTTAAGGCAGGCTAAGC TTTTAAATAAAGATAAATTATAAGAACTAGTTTCATTCATTAACTATTCTGCTATTACAA GTTACATCATCATGTTCATCTCCATAATACTAGGGCTAGCAGTTTGGTATTTAACACCAA ATCCTCCCAATGGGAGACATGTACTTTCTGGCTGAATTACAATTAACATCTTATTACTTT GGAACTGTATAATCAGGGGCCTGTTGCTCTCCTCATTAATGGAAAACAGCAGTCCAAAAT ATTACACTCTCCCCAGTAGAAGTACCATTATTATAGATTCAGAAAGGCTTTGCATATTCA CATTTTTGCAGTTTAATACTTGTGAAGATCAATGCAAGCTCTACACAGTGATAGTATTTA AACACATGCAATATTTCAAACTAAGGGGATCCAGCAGCCACTAATGTTTTCTAAGCACAC TTGAAAACATTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_012238 |
| Insert Size | 4100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012238.3, NP_036370.2 |
| RefSeq Size | 4107 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 23411 |
| UniProt ID | Q96EB6 |
| Domains | SIR2 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Picroside II attenuates hyperhomocysteinemia-induced endothelial injury by reducing inflammation, oxidative stress and cell apoptosis
,Wang, Y;Hong, Y;Zhang, C;Shen, Y;Pan, YS;Chen, RZ;Zhang, Q;Chen, YH;,
J. Cell. Mol. Med.
,PubMed ID 30394648
[SIRT1]
|
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Resveratrol inhibits epithelial-mesenchymal transition of retinal pigment epithelium and development of proliferative vitreoretinopathy
,Ishikawa, K;He, S;Terasaki, H;Nazari, H;Zhang, H;Spee, C;Kannan, R;Hinton, DR;,
Sci Rep
,PubMed ID 26552368
[SIRT1]
|
|
A potent and selective Sirtuin 1 inhibitor alleviates pathology in multiple animal and cell models of Huntington's disease
,Smith, MR;Syed, A;Lukacsovich, T;Purcell, J;Barbaro, BA;Worthge, SA;Wei, SR;Pollio, G;Magnoni, L;Scali, C;Massai, L;Franceschini, D;Camarri, M;Gianfriddo, M;Diodato, E;Thomas, R;Gokce, O;Tabrizi, SJ;Caricasole, A;Landwehrmeyer, B;Menalled, L;Murphy, C;Ramboz, S;Luthi-Carter, R;Westerberg, G;Marsh, JL;,
Hum. Mol. Genet., Jan 2014.
,PubMed ID 24436303
[SIRT1]
|
|
Overexpression of SIRT1 Protein in Neurons Protects against Experimental Autoimmune Encephalomyelitis through Activation of Multiple SIRT1 Targets
,Vamshi K. Nimmagadda, Christopher T. Bever, Narasimha R. Vattikunta, Saifi Talat, Vakas Ahmad, Naveen K. Nagalla, David Trisler, Susan I. V. Judge, Walter Royal, III, Krish Chandrasekaran, James W. Russell, and Tapas K. Makar,
J. Immunol., May 2013; 190: 4595 - 4607.
,PubMed ID 23547115
[SIRT1]
|
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The novel growth factor, progranulin, stimulates mouse cholangiocyte proliferation via sirtuin-1-mediated inactivation of FOXO1
,Gabriel Frampton, Yoshiyuki Ueno, Matthew Quinn, Matthew McMillin, Hae Yong Pae, Cheryl Galindo, Dinorah Leyva-Illades, and Sharon DeMorrow,
Am J Physiol Gastrointest Liver Physiol, Dec 2012; 303: G1202 - G1211.
[SIRT1]
|
|
Discovery, In Vivo Activity, and Mechanism of Action of a Small-Molecule p53 Activator
,null,
Cancer Cell
,PubMed ID 18455128
[SIRT1]
|
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Resources
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| RC218134L2 | Lenti ORF clone of Human sirtuin 1 (SIRT1), transcript variant 1, mGFP tagged |
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| RC218134L3 | Lenti ORF clone of Human sirtuin 1 (SIRT1), transcript variant 1, Myc-DDK-tagged |
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| RC218134L4 | Lenti ORF clone of Human sirtuin 1 (SIRT1), transcript variant 1, mGFP tagged |
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| RG218134 | SIRT1 (tGFP-tagged) - Human sirtuin 1 (SIRT1), transcript variant 1 |
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