SRP1 (KPNA1) (NM_002264) Human Untagged Clone

CAT#: SC128103

KPNA1 (untagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1



  "NM_002264" in other vectors (7)

CNY 7,220.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


KPNA1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms IPOA5; NPI-1; RCH2; SRP1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_002264, the custom clone sequence may differ by one or more nucleotides


ATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCCGATGAGA
TGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCG
GAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCT
CAGATTAGTAACATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCA
AAAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCC
TATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAAT
TGTACACTGCAGTTTGAATCAGCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTTCAGACCCGAA
TTGTGATTCAGGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCAGAGTTTGAAGATGTCCAGGA
ACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGTACCATGTGCAGGGACTATGTCTTAGACTGC
AATATCCTTCCCCCTCTTTTGCAGTTATTTTCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTAT
GGGCTTTGTCTAATCTCTGTAGAGGGAAAAGTCCACCTCCAGAATTTGCAAAGGTTTCTCCATGTCTGAA
TGTGCTTTCCTGGTTGCTGTTTGTCAGTGACACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATAT
CTATCAGATGGACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGACTTGTGGAAC
TGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTGGGAAACATTGTCACAGGGGA
TGATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTGCAGAGTTTATTGCATTTGCTGAGTAGCCCA
AAGGAATCTATCAAAAAGGAAGCATGTTGGACGATATCTAATATTACAGCTGGAAATAGGGCACAGATCC
AGACTGTGATAGATGCCAACATTTTCCCAGCCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAG
AAAAGAAGCAGCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTA
GAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTACAGGTTGCCC
TAAATGGCTTGGAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGGAATGGCACTGGCATTAACCC
TTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAAATTGAGTTCTTACAGAGTCATGAAAACCAG
GAGATCTACCAAAAGGCCTTTGATCTTATTGAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTG
CACCCCAGGTTGACCTTAACCAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGGAAGGTTTCCA
GCTTTGA


>OriGene 5' read for NM_002264 unedited
TGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGCTCGGTGCGAGGCGG
CGGAGAGCGAGGCCTGGTGAGCACCGCCGAGGCGCGGGCCAGCTCTTCGAGGTTGTGCGC
GGGAGTGGCACGGCGGGCGGGCGAGCGAGGGGCTAACTTCAGCGGTGGCACCGGGATCGG
TTGCCTTGAGCCTGAAATCATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTA
CAAGAACAAATCTCTGAATCCCGATGAGATGCGCAGGAGGAGGGAGGAAGAAGGACTGCA
GTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCGGAGAAATGTTGCTACAGCAGA
AGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCTCAGATTAATAA
CATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCAA
AAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACC
TAACCCTCCTATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTT
CCTCAAACGAAAAGAGAATTGTACCACTGCAGTTTGAATCAGCTTTGGGTACTGACAAAT
ATTGCTTCAGGAAATTCTCTTCAACCCGAATTGTGATTCAGCAGGAGCTGTGCCCATCTT
CATAGAGTTGCTCAGCTCAGAGTTTGGAAGATGTCCAGGAACAGGGCAGTCTGGGCTCTT
GGNCACATTGCTGNAGATAGTACCATGTGCAGGGACTATGTCTTAGACTGGCATATNCCT
TCCCCTCTTTTGCAGTTATTTTCAAGCAAAACGCCTGACCTGACCCGA
>OriGene 3' genomic read for NM_002264 unedited
NNNGGGCCCACTTGGGNGGTTGGCCACAATTCCCAGGGCCACAGNAATGAGCCACCTGGG
GNAGTGGGCTCACAGGGNATGCCCACCCGGGATTCTGTTCAGGAAACAGCCACGACCGCC
GGCCGCAATCTAGAGCCGACCCCCCCCCCCCCCCCCCCCCCCCCAGGAAAACCCCTTCAT
AATGCATCCCCCAATCCGGGGGATTAAATCGCTGAACTGCCTACTAGCACTAGAGAACTA
AACTCAAAGCCTGCCTGTGGGTAGACTTTACAATAGAACTGGTCTGACAAAGCTTGGTCT
TATAAAATATACTTACATATTGTTTCTTAGGCAAAAATCGGCAGCAATCTGGAATAATTC
AGATTTTTTATGAGATTAATTGCCAATCTTTTTATCTGTGACGATAATGCCAAAAATGAC
TCAAAGCCAATAACCTTCATCTGTCATATGGAAAAAACAATCCTAGGGCAATATTTTTTT
CAAAAGCGGAACTGACAAAGATAAGCGTTTAAAAGGGTCTTGATCAATTAGTTACAGGAA
CATGAAAGAAAGTGTTCCACAGAGAACTGTGTCTCCTAAAATCTCTTTCTTCTATCATCT
ACCTTACCCAAAATTTCTCAATCCAACCACATCTACCTGCTGGTCCATCAGATACCAGAT
GCCTTTACATCCAAAATTCTCTTAATGCTTTTTTTCCCCCACCAAAGTTCTCATTACAAA
TAATATAGATTTGNNAGTTTGGNAGGATGGAGGGGAATTAAAAAGGAAAAGTAAAACAAA
GTCCCTTTTCCCCAATCCTATATAAAACTACAAAGATTTTTCCTGTATTTGAATTAACAT
GCTTTTGTAATGAGCAACCCCTTATGGTAGGTTTTTCAGTGAATTTAAACTAAGTTAACA
GAATCTTAAGGCCCAAAT
Restriction Sites NotI-NotI     
ACCN NM_002264
Insert Size 4700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002264.2, NP_002255.2
RefSeq Size 6887 bp
RefSeq ORF 1617 bp
Locus ID 3836
UniProt ID P52294
Domains Armadillo_seg, IBB
Gene Summary The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Transcript Variant: This variant (1) lacks an alternate exon compared to variant 2 and represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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