SRP1 (KPNA1) (NM_002264) Human Untagged Clone
CAT#: SC128103
KPNA1 (untagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1
CNY 7,220.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | IPOA5; NPI-1; RCH2; SRP1 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_002264, the custom clone sequence may differ by one or more nucleotides
ATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCCGATGAGA TGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCG GAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCT CAGATTAGTAACATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCA AAAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCC TATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTTCCTCAAACGAAAAGAGAAT TGTACACTGCAGTTTGAATCAGCTTGGGTACTGACAAATATTGCTTCAGGAAATTCTCTTCAGACCCGAA TTGTGATTCAGGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCAGAGTTTGAAGATGTCCAGGA ACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGTACCATGTGCAGGGACTATGTCTTAGACTGC AATATCCTTCCCCCTCTTTTGCAGTTATTTTCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTAT GGGCTTTGTCTAATCTCTGTAGAGGGAAAAGTCCACCTCCAGAATTTGCAAAGGTTTCTCCATGTCTGAA TGTGCTTTCCTGGTTGCTGTTTGTCAGTGACACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATAT CTATCAGATGGACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGACTTGTGGAAC TGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTTGCGAGCTGTGGGAAACATTGTCACAGGGGA TGATATTCAGACACAGGTAATTCTGAATTGCTCAGCTCTGCAGAGTTTATTGCATTTGCTGAGTAGCCCA AAGGAATCTATCAAAAAGGAAGCATGTTGGACGATATCTAATATTACAGCTGGAAATAGGGCACAGATCC AGACTGTGATAGATGCCAACATTTTCCCAGCCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAG AAAAGAAGCAGCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAAGTACCTAGTA GAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGGTCATGGACTCTAAGATTGTACAGGTTGCCC TAAATGGCTTGGAAAATATCCTGAGGCTTGGAGAACAGGAAGCCAAAAGGAATGGCACTGGCATTAACCC TTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAAATTGAGTTCTTACAGAGTCATGAAAACCAG GAGATCTACCAAAAGGCCTTTGATCTTATTGAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTG CACCCCAGGTTGACCTTAACCAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGGAAGGTTTCCA GCTTTGA >OriGene 5' read for NM_002264 unedited
TGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGCTCGGTGCGAGGCGG CGGAGAGCGAGGCCTGGTGAGCACCGCCGAGGCGCGGGCCAGCTCTTCGAGGTTGTGCGC GGGAGTGGCACGGCGGGCGGGCGAGCGAGGGGCTAACTTCAGCGGTGGCACCGGGATCGG TTGCCTTGAGCCTGAAATCATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTA CAAGAACAAATCTCTGAATCCCGATGAGATGCGCAGGAGGAGGGAGGAAGAAGGACTGCA GTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCGGAGAAATGTTGCTACAGCAGA AGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCTCAGATTAATAA CATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCAA AAGCCCAGAGCAACAGCTTTCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACC TAACCCTCCTATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGTT CCTCAAACGAAAAGAGAATTGTACCACTGCAGTTTGAATCAGCTTTGGGTACTGACAAAT ATTGCTTCAGGAAATTCTCTTCAACCCGAATTGTGATTCAGCAGGAGCTGTGCCCATCTT CATAGAGTTGCTCAGCTCAGAGTTTGGAAGATGTCCAGGAACAGGGCAGTCTGGGCTCTT GGNCACATTGCTGNAGATAGTACCATGTGCAGGGACTATGTCTTAGACTGGCATATNCCT TCCCCTCTTTTGCAGTTATTTTCAAGCAAAACGCCTGACCTGACCCGA >OriGene 3' genomic read for NM_002264 unedited
NNNGGGCCCACTTGGGNGGTTGGCCACAATTCCCAGGGCCACAGNAATGAGCCACCTGGG GNAGTGGGCTCACAGGGNATGCCCACCCGGGATTCTGTTCAGGAAACAGCCACGACCGCC GGCCGCAATCTAGAGCCGACCCCCCCCCCCCCCCCCCCCCCCCCAGGAAAACCCCTTCAT AATGCATCCCCCAATCCGGGGGATTAAATCGCTGAACTGCCTACTAGCACTAGAGAACTA AACTCAAAGCCTGCCTGTGGGTAGACTTTACAATAGAACTGGTCTGACAAAGCTTGGTCT TATAAAATATACTTACATATTGTTTCTTAGGCAAAAATCGGCAGCAATCTGGAATAATTC AGATTTTTTATGAGATTAATTGCCAATCTTTTTATCTGTGACGATAATGCCAAAAATGAC TCAAAGCCAATAACCTTCATCTGTCATATGGAAAAAACAATCCTAGGGCAATATTTTTTT CAAAAGCGGAACTGACAAAGATAAGCGTTTAAAAGGGTCTTGATCAATTAGTTACAGGAA CATGAAAGAAAGTGTTCCACAGAGAACTGTGTCTCCTAAAATCTCTTTCTTCTATCATCT ACCTTACCCAAAATTTCTCAATCCAACCACATCTACCTGCTGGTCCATCAGATACCAGAT GCCTTTACATCCAAAATTCTCTTAATGCTTTTTTTCCCCCACCAAAGTTCTCATTACAAA TAATATAGATTTGNNAGTTTGGNAGGATGGAGGGGAATTAAAAAGGAAAAGTAAAACAAA GTCCCTTTTCCCCAATCCTATATAAAACTACAAAGATTTTTCCTGTATTTGAATTAACAT GCTTTTGTAATGAGCAACCCCTTATGGTAGGTTTTTCAGTGAATTTAAACTAAGTTAACA GAATCTTAAGGCCCAAAT |
Restriction Sites | NotI-NotI |
ACCN | NM_002264 |
Insert Size | 4700 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002264.2, NP_002255.2 |
RefSeq Size | 6887 bp |
RefSeq ORF | 1617 bp |
Locus ID | 3836 |
UniProt ID | P52294 |
Domains | Armadillo_seg, IBB |
Gene Summary | The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (1) lacks an alternate exon compared to variant 2 and represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210333 | KPNA1 (Myc-DDK-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 |
CNY 6,008.00 |
|
RC210333L1 | Lenti-ORF clone of KPNA1 (Myc-DDK-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 |
CNY 8,408.00 |
|
RC210333L2 | Lenti-ORF clone of KPNA1 (mGFP-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 |
CNY 5,890.00 |
|
RC210333L3 | Lenti-ORF clone of KPNA1 (Myc-DDK-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 |
CNY 8,408.00 |
|
RC210333L4 | Lenti-ORF clone of KPNA1 (mGFP-tagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 |
CNY 8,408.00 |
|
RG210333 | KPNA1 (tGFP-tagged) - Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 |
CNY 7,608.00 |
|
SC322070 | KPNA1 (untagged)-Human karyopherin alpha 1 (importin alpha 5) (KPNA1), transcript variant 1 |
CNY 6,016.00 |