Nogo A (RTN4) (NM_153828) Human Untagged Clone

CAT#: SC128187

RTN4 (untagged)-Human reticulon 4 (RTN4), transcript variant 2



  "NM_153828" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
RTN4 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ASY; Nbla00271; Nbla10545; NI220/250; NOGO; NSP; NSP-CL; RTN-X; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC128187 sequence for NM_153828 edited (data generated by NextGen Sequencing)
ATGGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCCGCAG
CCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAAGAGGAGGAG
GAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCCCGCC
GCCGGGCTGTCCGCGGCCCCAGTGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGATGGAC
TTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCCCGTC
GCCCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGCGCCA
TCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGACGAGCCTCCG
GCCCGGCCTCCCCCTCCTCCCCCGGCCAGCGTGAGCCCCCAGGCAGAGCCCGTGTGGACC
CCGCCAGCCCCGGCTCCCGCCGCGCCCCCCTCCACCCCGGCCGCGCCCAAGCGCAGGGGC
TCCTCGGGCTCAGTGGTTGTTGACCTCCTGTACTGGAGAGACATTAAGAAGACTGGAGTG
GTGTTTGGTGCCAGCCTATTCCTGCTGCTTTCATTGACAGTATTCAGCATTGTGAGCGTA
ACAGCCTACATTGCCTTGGCCCTGCTCTCTGTGACCATCAGCTTTAGGATATACAAGGGT
GTGATCCAAGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATCTGGAATCT
GAAGTTGCTATATCTGAGGAGTTGGTTCAGAAGTACAGTAATTCTGCTCTTGGTCATGTG
AACTGCACGATAAAGGAACTCAGGCGCCTCTTCTTAGTTGATGATTTAGTTGATTCTCTG
AAGTTTGCAGTGTTGATGTGGGTATTTACCTATGTTGGTGCCTTGTTTAATGGTCTGACA
CTACTGATTTTGGCTCTCATTTCACTCTTCAGTGTTCCTGTTATTTATGAACGGCATCAG
GCACAGATAGATCATTATCTAGGACTTGCAAATAAGAATGTTAAAGATGCTATGGCTAAA
ATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCTGAATGA

Clone variation with respect to NM_153828.2
>OriGene 5' read for NM_153828 unedited
TTGGATTTTGTAATACGACTCTCTATTAGGGCGGCCGCGAATTCGCACGAGCCACCCTCC
AGCCATGGAAGACCTGGACCAGTCTCCTCTGGTCTCGTCCTCGGACAGCCCACCCCGGCC
GCAGCCCGCGTTCAAGTACCAGTTCGTGAGGGAGCCCGAGGACGAGGAGGAAGAAGAGGA
GGAGGAAGAGGAGGACGAGGACGAAGACCTGGAGGAGCTGGAGGTGCTGGAGAGGAAGCC
CGCCGCCGGGCTGTCCGCGGCCCCAGTGCCCACCGCCCCTGCCGCCGGCGCGCCCCTGAT
GGACTTCGGAAATGACTTCGTGCCGCCGGCGCCCCGGGGACCCCTGCCGGCCGCTCCCCC
CGTCGCCCCGGAGCGGCAGCCGTCTTGGGACCCGAGCCCGGTGTCGTCGACCGTGCCCGC
GCCATCCCCGCTGTCTGCTGCCGCAGTCTCGCCCTCCAAGCTCCCTGAGGACGACGAGCC
TCCGGCCCGGGCCTCCCCCTTCTTCCCCGGNCAGNGNGAGCCCCCNAGCAGAGCCCGNGN
GGACCCCGCCAGCCCCGGCTTCCGGCGNGCCCCCCNCCACCCCGGCCGCGCCCAAACGCA
GGGGCNCCCCGGCCCCAGGGGNGGGGGACCCCCCGGACGGGAAAGACACAAAAAGACCGG
AGGGGCGGCNGGNGCCCACCCAANCCCGCCGCCCACAGGCAGGACCCACGCAGGGGAGCG
GAACAGCCCACAANGGCCGGGGCCCGGCCCCCGGACCAACAACCCGAAGGAAAACAGGGG
GGGAACCAAGCGCCCCAAAAAAAAGAGGAGGGCCCCCCACCCGGGGCAAANNGGGACCCC
GCAACGGCCCAACCCGAAGGGGGGGGCACAAAGACACCAAACCCGGCCCTGGACAAGGGA
GCCGGCCAAAAAGCAACCCAGGCCCCCCNNCCAACGGCGACCCAACAGACCCCC
>OriGene 3' read for NM_153828 unedited
GACTCGGATTTTTTTCTTTTTTTTTTTCTAGCTCCACCATCTCTGCAACTTGCCAANATG
CGGCAAGACTATCTGCAACAAAGTAAAATATACAGGTTTTTTATTCCACCAGTGCCTCAG
ATTAGATAGGAAAAAGATATGATTACGGTTTAAATCCATACATAGCAGCTTACAATACTT
AAGATGATGAACACATGGCAGTCAAGACAGGTAATTTTTCCTCACAACAGTGCATGGCTA
AAAATAAAGATCTAACAACGATCTGTGAAACTGCACTGCAACGTCAAGGTTCGTTCTTCC
CTGACCCTCCCCCGTATAATCAAATGAATATCCCCTTTAAAGATGAACTCCTACTAATTA
TTTTGGGCGTTTTCATTCAGCTTTGCGCTTCAATCCAGGGATTTTTGCTTGGATTTTAGC
CATAGCATCTTTAACATTCTTATTTGCAAGTCCTAGATAATGATCTATCTGTGCCTGATG
CCGTTCATAAATAACAGGGACACTGAAGAGTGAAAGGAGAGCCAAAATCAGTGGTGTCAG
CCCATTAAACAAGGCCCCAACTTAGGGAAATACCCACATTAACCCTGCAAACTTTAGGGA
ATCAACTTAATCATCCACTAAGAAGAGGCCCCTGGGTTCCTTTTTCGGGGCAGTTACCTG
ACCCAGGGCGGAATTCCTGTACTTTTGAACCAACTCCTCAGATTTGGAACCTTAGATTCC
CAGTTTTGCCCTGGAGGGGGGGGCCTTCAACTGGATTCTTGGGAAGGTTGGGACACACCC
TTTGGTTTTCCAAAAGCTGTTGGGAACAGGAGGCAGGCCCAGGGAATGGTAGGCTGGAAC
CCCCCCCAAGGTTGAATCCT
Restriction Sites NotI-NotI     
ACCN NM_153828
Insert Size 1700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153828.2, NP_722550.1
RefSeq Size 2414 bp
RefSeq ORF 1122 bp
Locus ID 57142
UniProt ID Q9NQC3
Protein Families Transmembrane
Gene Summary This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2), also known as Foocen-M, Nogo-B, RTN-XS and RTN4-B1, lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (B), that is missing an internal segment, compared to isoform A.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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