United States
Germany
Japan
United Kingdom
China
COX2 (PTGS2) (NM_000963) Human Untagged Clone
CAT＃: SC128243
PTGS2 (untagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2)
CNY 6,752.00
| Cited in 6 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COX-2; COX2; GRIPGHS; hCox-2; PGG/HS; PGHS-2; PHS-2 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_000963, the custom clone sequence may differ by one or more nucleotides
ATGCTCGCCCGCGCCCTGCTGCTGTGCGCGGTCCTGGCGCTCAGCCATACAGCAAATCCTTGCTGTTCCC ACCCATGTCAAAACCGAGGTGTATGTATGAGTGTGGGATTTGACCAGTATAAGTGCGATTGTACCCGGAC AGGATTCTATGGAGAAAACTGCTCAACACCGGAATTTTTGACAAGAATAAAATTATTTCTGAAACCCACT CCAAACACAGTGCACTACATACTTACCCACTTCAAGGGATTTTGGAACGTTGTGAATAACATTCCCTTCC TTCGAAATGCAATTATGAGTTATGTGTTGACATCCAGATCACATTTGATTGACAGTCCACCAACTTACAA TGCTGACTATGGCTACAAAAGCTGGGAAGCCTTCTCTAACCTCTCCTATTATACTAGAGCCCTTCCTCCT GTGCCTGATGATTGCCCGACTCCCTTGGGTGTCAAAGGTAAAAAGCAGCTTCCTGATTCAAATGAGATTG TGGAAAAATTGCTTCTAAGAAGAAAGTTCATCCCTGATCCCCAGGGCTCAAACATGATGTTTGCATTCTT TGCCCAGCACTTCACGCATCAGTTTTTCAAGACAGATCATAAGCGAGGGCCAGCTTTCACCAACGGGCTG GGCCATGGGGTGGACTTAAATCATATTTACGGTGAAACTCTGGCTAGACAGCGTAAACTGCGCCTTTTCA AGGATGGAAAAATGAAATATCAGATAATTGATGGAGAGATGTATCCTCCCACAGTCAAAGATACTCAGGC AGAGATGATCTACCCTCCTCAAGTCCCTGAGCATCTACGGTTTGCTGTGGGGCAGGAGGTCTTTGGTCTG GTGCCTGGTCTGATGATGTATGCCACAATCTGGCTGCGGGAACACAACAGAGTATGCGATGTGCTTAAAC AGGAGCATCCTGAATGGGGTGATGAGCAGTTGTTCCAGACAAGCAGGCTAATACTGATAGGAGAGACTAT TAAGATTGTGATTGAAGATTATGTGCAACACTTGAGTGGCTATCACTTCAAACTGAAATTTGACCCAGAA CTACTTTTCAACAAACAATTCCAGTACCAAAATCGTATTGCTGCTGAATTTAACACCCTCTATCACTGGC ATCCCCTTCTGCCTGACACCTTTCAAATTCATGACCAGAAATACAACTATCAACAGTTTATCTACAACAA CTCTATATTGCTGGAACATGGAATTACCCAGTTTGTTGAATCATTCACCAGGCAAATTGCTGGCAGGGTT GCTGGTGGTAGGAATGTTCCACCCGCAGTACAGAAAGTATCACAGGCTTCCATTGACCAGAGCAGGCAGA TGAAATACCAGTCTTTTAATGAGTACCGCAAACGCTTTATGCTGAAGCCCTATGAATCATTTGAAGAACT TACAGGAGAAAAGGAAATGTCTGCAGAGTTGGAAGCACTCTATGGTGACATCGATGCTGTGGAGCTGTAT CCTGCCCTTCTGGTAGAAAAGCCTCGGCCAGATGCCATCTTTGGTGAAACCATGGTAGAAGTTGGAGCAC CATTCTCCTTGAAAGGACTTATGGGTAATGTTATATGTTCTCCTGCCTACTGGAAGCCAAGCACTTTTGG TGGAGAAGTGGGTTTTCAAATCATCAACACTGCCTCAATTCAGTCTCTCATCTGCAATAACGTGAAGGGC TGTCCCTTTACTTCATTCAGTGTTCCAGATCCAGAGCTCATTAAAACAGTCACCATCAATGCAAGTTCTT CCCGCTCCGGACTAGATGATATCAATCCCACAGTACTACTAAAAGAACGTTCGACTGAACTGTAG >OriGene 5' read for NM_000963 unedited
AATCCGCCCGTTGCCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT TCGGCACGAGGCTTATAAAAAGGAAGGTTCTCTCGGTTAGCGACCAATTGTCATACGACT TGCAGTGAGCGTCAGGAGCACGTCCAGGAACTCCTCAGCAGCGCCTCCTTCAGCTCCACA GCCAGACGCCCTCAGACAGCAAAGCCTACCCCCGCGCCGCGCCCTGCCCGCCGCTGCGAT GCTCGCCCGCGCCCTGCTGCTGTGCGCGGTCCTGGCGCTCAGCCATACAGCAAATCCTTG CTGTTCCCACCCATGTCAAAACCGAGGTGTATGTATGAGTGTGGGATTTGACCAGTATAA GTGCGATTGTACCCGGACAGGATTCTATGGAGAAAACTGCTCAACACCGGAATTTTTGAC AAGAATAAATTATTTCTGAAACCCACTCCAACACAGTGCACTACATACTTACCCACTTCA GGGATTTTGGACCGTGTGATAACATTCCCTTTCTTCNAATGCAATATTAGTATGTGGTGA CATTCAGACACACTTTGATGACAGTTCCACACCTACAATGCTGCCTATGGGTACCAAAGC TGGGAGGCCTTTTTAACCTTCCTATATACTATAGCCCTTTCTCCTGTGCCTGAGAATGCC CGATCCCTTGTTGGTAAAGTAAAAACAGCTTCTGTTAAAAGAAAAGTGGAAAAATGCTTT TAAAAAAAATAATCCTGTTCCCCGGCCCAACAGAGGTTGCGTTTTTTGCCCCCTTTCCCC CACTTTTTAAGAAAAAAATACAAGGCCGTTTTCCACGGCTTGGCTGGGGGGGGTCTAAAA ATTTTCCGGGAATCTGGTTCACGCGC >Forward primer walk for NM_000963 unedited
ATNCTGNACCAGNAATACAACTATCAACAGTTTATCTACAACAACTCTTATTGCTGGAAC ATGGAATTACCCAGTTTGTTGAATCATTCACCAGGCAAATTGCTGGCAGGGTTGCTGGTG GTAGGAATGTTCCACCCGCAGTACAGAAAGTATCACAGGCTTCCATTGACCAGAGCAGGC AGATGAAATACCAGTCTTTTAATGAGTACCGCAAACGCTTTATGCTGAAGCCCTATGAAT CATTTGAAGAACTTACAGGAGAAAAGGAAATGTCTGCAGAGTTGGAAGCACTCTATGGTG ACATCGATGCTGTGGAGCTGTATCCTGCCCTTCTGGTAGAAAAGCCTCGGCCAGATGCCA TCTTTGGTGAAACCATGGTAGAAGTTGGAGCACCATTCTCCTTGAAAGGACTTATGGGTA ATGTTATATGTTCTCCTGCCTACTGGAAGCCAAGCACTTTTGGTGGAGAAGTGGGTTTTC AAATCATCAACACTGCCTCAATTCAGTCTCTCATCTGCAATAACGTGAAGGGCTGTCCCT TTACTTCATTCAGTGTTCCAGATCCAGAGCTCATTAAAACAGTCACCATCAATGNCAGTT CTTCCCGCTCCNGACTAGATGATATCAATCCCACAGTACTACTAANAGAACGTTCGACTG AACTGTAGAAGTCTAATGATCATATTTANTTATTTATATGAACCATGTCTATTATTTAAN NTATTAAATATATTTATATAAAACTCCNTATGNTACTTAAACATCTNCTGTAACAGAAGT CAGTACTCCTGGTGCCGAAAAAGGAGTCACTACTGTGAAGACTTTTATGTACTACTCTAA AGATTTGCTGTTGCTGNTAAGTTTGGAAAACAGTTTTATA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000963 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | A TrueClone. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000963.1, NP_000954.1 |
| RefSeq Size | 4465 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 5743 |
| UniProt ID | P35354 |
| Domains | An_peroxidase, EGF |
| Protein Families | Druggable Genome |
| Protein Pathways | Arachidonic acid metabolism, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway |
| Gene Summary | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009] |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hormophysa triquerta polyphenol, an elixir that deters CXCR4- and COX2-dependent dissemination destiny of treatment-resistant pancreatic cancer cells
,Aravindan, S;Ramraj, S;Kandasamy, K;Thirugnanasambandan, SS;Somasundaram, DB;Herman, TS;Aravindan, N;,
Oncotarget
,PubMed ID 27974694
[PTGS2]
|
|
Influenza A viruses suppress cyclooxygenase-2 expression by affecting its mRNA stability
,Dudek, SE;Nitzsche, K;Ludwig, S;Ehrhardt, C;,
Sci Rep
,PubMed ID 27265729
[PTGS2]
|
|
P50-associated COX-2 extragenic RNA (PACER) overexpression promotes proliferation and metastasis of osteosarcoma cells by activating COX-2 gene
,Qian, M;Yang, X;Li, Z;Jiang, C;Song, D;Yan, W;Liu, T;Wu, Z;Kong, J;Wei, H;Xiao, J;,
Tumour Biol.
,PubMed ID 26476537
[PTGS2]
|
|
SIRT6 Promotes COX-2 Expression and Acts as an Oncogene in Skin Cancer
,Ming, M;Han, W;Zhao, B;Sundaresan, NR;Deng, CX;Gupta, MP;He, YY;,
Cancer Res.
,PubMed ID 25320180
[PTGS2]
|
|
Cyclooxygenase-2 Enzyme Induces the Expression of the a-2,3-Sialyltransferase-3 (ST3Gal-I) in Breast Cancer
,Daisy Sproviero, Sylvain Julien, Brian Burford, Joyce Taylor-Papadimitriou, and Joy M. Burchell,
J. Biol. Chem., Dec 2012; 287: 44490 - 44497.
[PTGS2]
|
|
The ß-Arrestin Pathway-selective Type 1A Angiotensin Receptor (AT1A) Agonist [Sar1,Ile4,Ile8]Angiotensin II Regulates a Robust G Protein-independent Signaling Network
,Ryan T. Kendall, Erik G. Strungs, Saleh M. Rachidi, Mi-Hye Lee, Hesham M. El-Shewy, Deirdre K. Luttrell, Michael G. Janech, and Louis M. Luttrell,
J. Biol. Chem., Jun 2011; 286: 19880 - 19891
[PTGS2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202245 | PTGS2 (Myc-DDK-tagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) |
CNY 6,744.00 |
|
| RC202245L1 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), Myc-DDK-tagged |
CNY 9,144.00 |
|
| RC202245L2 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mGFP tagged |
CNY 9,144.00 |
|
| RC202245L3 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), Myc-DDK-tagged |
CNY 9,144.00 |
|
| RC202245L4 | Lenti ORF clone of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), mGFP tagged |
CNY 9,144.00 |
|
| RG202245 | PTGS2 (tGFP-tagged) - Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) |
CNY 8,344.00 |
|
| SC323764 | PTGS2 (untagged)-Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2) |
CNY 6,752.00 |
