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IDUA (NM_000203) Human Untagged Clone
CAT＃: SC300024
IDUA (untagged)-Human iduronidase, alpha-L- (IDUA)
CNY 7,304.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IDA; MPS1; MPSI |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000203 edited
GAATTCCGCGTGGCCATGCGTCCCCTGCGCCCCCGCGCCGCGCTGCTGGCGCTCCTGGCC TCGCTCCTGGCCGCGCCCCCGGTGGCCCCGGCCGAGGCCCCGCACCTGGTGCATGTGGAC GCGGCCCGCGCGCTGTGGCCCCTGCGGCGCTTCTGGAGGAGCACAGGCTTCTGCCCCCCG CTGCCACACAGCCAGGCTGACCAGTACGTCCTCAGCTGGGACCAGCAGCTCAACCTCGCC TATGTGGGCGCCGTCCCTCACCGCGGCATCAAGCAGGTCCGGACCCACTGGCTGCTGGAG CTTGTCACCACCAGGGGGTCCACTGGACGGGGCCTGAGCTACAACTTCACCCACCTGGAC GGGTACCTGGACCTTCTCAGGGAGAACCAGCTCCTCCCAGGGTTTGAGCTGATGGGCAGC GCCTCGGGCCACTTCACTGACTTTGAGGACAAGCAGCAGGTGTTTGAGTGGAAGGACTTG GTCTCCAGCCTGGCCAGGAGATACATCGGTAGGTACGGACTGGCGCATGTTTCCAAGTGG AACTTCGAGACGTGGAATGAGCCAGACCACCACGACTTTGACAACGTCTCCATGACCATG CAAGGCTTCCTGAACTACTACGATGCCTGCTCGGAGGGTCTGCGCGCCGCCAGCCCCGCC CTGCGGCTGGGAGGCCCCGGCGACTCCTTCCACACCCCACCGCGATCCCCGCTGAGCTGG GGCCTCCTGCGCCACTGCCACGACGGTACCAACTTCTTCACTGGGGAGGCGGGCGTGCGG CTGGACTACATCTCCCTCCACAGGAAGGGTGCGCGCAGCTCCATCTCCATCCTGGAGCAG GAGAAGGTCGTCGCGCAGCAGATCCGGCAGCTCTTCCCCAAGTTCGCGGACACCCCCATT TACAACGACGAGGCGGACCCGCTGGTGGGCTGGTCCCTGCCACAGCCGTGGAGGGCGGAC GTGACCTACGCGGCCATGGTGGTGAAGGTCATCGCGCAGCATCAGAACCTGCTACTGGCC AACACCACCTCCGCCTTCCCCTACGCGCTCCTGAGCAACGACAATGCCTTCCTGAGCTAC CACCCGCACCCCTTCGCGCAGCGCACGCTCACCGCGCGCTTCCAGGTCAACAACACCCGC CCGCCGCACGTGCAGCTGTTGCGCAAGCCGGTGCTCACGGCCATGGGGCTGCTGGCGCTG CTGGATGAGGAGCAGCTCTGGGCCGAAGTGTCGCAGGCCGGGACCGTCCTGGACAGCAAC CACACGGTGGGCGTCCTGGCCAGCGCCCACCGCCCCCAGGGCCCGGCCGACGCCTGGCGC GCCGCGGTGCTGATCTACGCGAGCGACGACACCCGCGCCCACCCCAACCGCAGCGTCGCG GTGACCCTGCGGCTGCGCGGGGTGCCCCCCGGCCCGGGCCTGGTCTACGTCACGCGCTAC CTGGACAACGGGCTCTGCAGCCCCGACGGCGAGTGGCGGCGCCTGGGCCGGCCCGTCTTC CCCACGGCAGAGCAGTTCCGGCGCATGCGCGCGGCTGAGGACCCGGTGGCCGCGGCGCCC CGCCCCTTACCCGCCGGCGGCCGCCTGACCCTGCGCCCCGCGCTGCGGCTGCCGTCGCTT TTGCTGGTGCACGTGTGTGCGCGCCCCGAGAAGCCGCCCGGGCAGGTCACGCGGCTCCGC GCCCTGCCCCTGACCCAAGGGCAGCTGGTTCTGGTCTGGTCGGATGAACACGTGGGCTCC AAGTGCCTGTGGACATACGAGATCCAGTTCTCTCAGGACGGTAAGGCGTACACCCCGGTC AGCAGGAAGCCATCGACCTTCAACCTCTTTGTGTTCAGCCCAGACACAGGTGCTGTCTCT GGCTCCTACCGAGTTCGAGCCCTGGACTACTGGGCCCGACCAGGCCCCTTCTCGGACCCT GTGCCGTACCTGGAGGTCCCTGTGCCAAGAGGGCCCCCATCCCCGGGCAATCCATGAGCC TGTGCTGAGCCCCAGTGGGTTGCACCTCCACCGGCAGTCAGCGAGCTGGGGCTGCACTGT GCCCATGCTGCCCTCCCATCACCCCCTTTGCAATATATTTTTATATTTTTTTAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000203 |
| Insert Size | 2100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this clone has been fully sequenced and found to be a perfect match to the protein associated with this reference, NM_000203.3. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000203.2, NP_000194.1 |
| RefSeq Size | 2197 bp |
| RefSeq ORF | 1962 bp |
| Locus ID | 3425 |
| UniProt ID | P35475 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycosaminoglycan degradation, Lysosome, Metabolic pathways |
| Gene Summary | This gene encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Mutations in this gene that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the functional protein. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219336 | IDUA (Myc-DDK-tagged)-Human iduronidase, alpha-L- (IDUA) |
CNY 7,288.00 |
|
| RC219336L1 | Lenti ORF clone of Human iduronidase, alpha-L- (IDUA), Myc-DDK-tagged |
CNY 9,688.00 |
|
| RC219336L2 | Lenti ORF clone of Human iduronidase, alpha-L- (IDUA), mGFP tagged |
CNY 7,600.00 |
|
| RC219336L3 | Lenti ORF clone of Human iduronidase, alpha-L- (IDUA), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC219336L4 | Lenti ORF clone of Human iduronidase, alpha-L- (IDUA), mGFP tagged |
CNY 9,688.00 |
|
| RG219336 | IDUA (tGFP-tagged) - Human iduronidase, alpha-L- (IDUA) |
CNY 8,888.00 |
