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Plakophilin 1 (PKP1) (NM_000299) Human Untagged Clone
CAT＃: SC300042
PKP1 (untagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1b
CNY 5,480.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | B6P; EDSFS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC300042 representing NM_000299.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACCACTCGCCGCTCAAGACCGCCTTGGCGTACGAATGCTTCCAGGACCAGGACAACTCCACGTTG GCTTTGCCGTCGGACCAAAAGATGAAAACAGGCACGTCTGGCAGGCAGCGCGTGCAGGAGCAGGTGATG ATGACCGTCAAGCGGCAGAAGTCCAAGTCTTCCCAGTCGTCCACCCTGAGCCACTCCAATCGAGGTTCC ATGTATGATGGCTTGGCTGACAATTACAACTATGGGACCACCAGCAGGAGCAGCTACTACTCCAAGTTC CAGGCAGGGAATGGCTCATGGGGATATCCGATCTACAATGGAACCCTCAAGCGGGAGCCTGACAACAGG CGCTTCAGCTCCTACAGCCAGATGGAGAACTGGAGCCGGCACTACCCCCGGGGCAGCTGTAACACCACC GGCGCAGGCAGCGACATCTGCTTCATGCAGAAAATCAAGGCGAGCCGCAGTGAGCCCGACCTCTACTGT GACCCACGGGGCACCCTGCGCAAGGGCACGCTGGGCAGCAAGGGCCAGAAGACCACCCAGAACCGCTAC AGCTTTTACAGCACCTGCAGTGGTCAGAAGGCCATAAAGAAGTGCCCTGTGCGCCCGCCCTCTTGTGCC TCCAAGCAGGACCCTGTGTATATCCCGCCCATCTCCTGCAACAAGGACCTGTCCTTTGGCCACTCTAGG GCCAGCTCCAAGATCTGCAGTGAGGACATCGAGTGCAGTGGGCTGACCATCCCCAAGGCTGTGCAGTAC CTGAGCTCCCAGGATGAGAAGTACCAGGCCATTGGGGCCTATTACATCCAGCATACCTGCTTCCAGGAT GAATCTGCCAAGCAACAGGTCTATCAGCTGGGAGGCATCTGCAAGCTGGTGGACCTCCTCCGCAGCCCC AACCAGAACGTCCAGCAGGCCGCGGCAGGGGCCCTGCGCAACCTGGTGTTCAGGAGCACCACCAACAAG CTGGAGACCCGGAGGCAGAATGGGATCCGCGAGGCAGTCAGCCTCCTGAGGAGAACCGGGAACGCCGAG ATCCAGAAGCAGCTGACTGGGCTGCTCTGGAACCTGTCTTCCACTGACGAGCTGAAGGAGGAACTCATT GCCGACGCCCTGCCTGTTCTGGCCGACCGCGTCATCATTCCCTTCTCTGGCTGGTGCGATGGCAATAGC AACATGTCCCGGGAAGTGGTGGACCCTGAGGTCTTCTTCAATGCCACAGGCTGCTTGAGAAAGAGACTG GGCATGCGGGAGCTTCTGGCTCTTGTTCCGCAAAGGGCCACTAGTAGCAGGGTGAACCTGAGCTCGGCC GATGCAGGCCGCCAGACCATGCGTAACTACTCAGGGCTCATTGATTCCCTCATGGCCTATGTCCAGAAC TGTGTAGCGGCCAGCCGCTGTGACGACAAGTCTGTGGAAAACTGCATGTGTGTTCTGCACAACCTCTCC TACCGCCTGGACGCCGAGGTGCCCACCCGCTACCGCCAGCTGGAGTATAACGCCCGCAACGCCTACACC GAGAAGTCCTCCACTGGCTGCTTCAGCAACAAGAGCGACAAGATGATGAACAACAACTATGACTGCCCC CTGCCTGAGGAAGAGACCAACCCCAAGGGCAGCGGCTGGTTGTACCATTCAGATGCCATCCGCACCTAC CTGAACCTCATGGGCAAGAGCAAGAAAGATGCTACCCTGGAGGCCTGTGCTGGTGCCCTGCAGAACCTG ACAGCCAGCAAGGGGCTGATGTCCAGTGGCATGAGCCAGTTGATTGGGCTGAAGGAAAAGGGCCTGCCA CAAATTGCCCGCCTCCTGCAATCTGGCAACTCTGATGTGGTGCGGTCCGGAGCCTCCCTCCTGAGCAAC ATGTCCCGCCACCCTCTGCTGCACAGAGTGATGGGGAACCAGGTGTTCCCGGAGGTGACCAGGCTCCTC ACCAGCCACACTGGCAATACCAGCAACTCCGAAGACATCTTGTCCTCGGCCTGCTACACTGTGAGGAAC CTGATGGCCTCGCAGCCACAACTGGCCAAGCAGTACTTCTCCAGCAGCATGCTCAACAACATCATCAAC CTGTGCCGAAGCAGTGCCTCACCCAAGGCCGCAGAAGCTGCCCGGCTTCTCCTGTCTGACATGTGGTCC AGCAAGGAACTGCAGGGTGTCCTCAGACAGCAAGGTTTCGATAGGAACATGCTGGGAACCTTAGCTGGG GCCAACAGCCTCAGGAACTTCACCTCCCGATTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_000299 |
| Insert Size | 2244 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000299.2 |
| RefSeq Size | 5447 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 5317 |
| UniProt ID | Q13835 |
| Protein Families | Druggable Genome |
| MW | 82.9 kDa |
| Gene Summary | This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] Transcript Variant: This variant (1b) represents the longer transcript variant and encodes the longer protein (isoform 1b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217943 | PKP1 (Myc-DDK-tagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1b |
CNY 7,890.00 |
|
| RC217943L3 | Lenti-ORF clone of PKP1 (Myc-DDK-tagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1b |
CNY 9,790.00 |
|
| RC217943L4 | Lenti-ORF clone of PKP1 (mGFP-tagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1b |
CNY 9,790.00 |
|
| RG217943 | PKP1 (tGFP-tagged) - Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1b |
CNY 8,650.00 |
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