Plakophilin 1 (PKP1) (NM_000299) Human Untagged Clone

CAT#: SC300042

PKP1 (untagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1b



  "NM_000299" in other vectors (4)

CNY 5,480.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms B6P; EDSFS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC300042 representing NM_000299.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAACCACTCGCCGCTCAAGACCGCCTTGGCGTACGAATGCTTCCAGGACCAGGACAACTCCACGTTG
GCTTTGCCGTCGGACCAAAAGATGAAAACAGGCACGTCTGGCAGGCAGCGCGTGCAGGAGCAGGTGATG
ATGACCGTCAAGCGGCAGAAGTCCAAGTCTTCCCAGTCGTCCACCCTGAGCCACTCCAATCGAGGTTCC
ATGTATGATGGCTTGGCTGACAATTACAACTATGGGACCACCAGCAGGAGCAGCTACTACTCCAAGTTC
CAGGCAGGGAATGGCTCATGGGGATATCCGATCTACAATGGAACCCTCAAGCGGGAGCCTGACAACAGG
CGCTTCAGCTCCTACAGCCAGATGGAGAACTGGAGCCGGCACTACCCCCGGGGCAGCTGTAACACCACC
GGCGCAGGCAGCGACATCTGCTTCATGCAGAAAATCAAGGCGAGCCGCAGTGAGCCCGACCTCTACTGT
GACCCACGGGGCACCCTGCGCAAGGGCACGCTGGGCAGCAAGGGCCAGAAGACCACCCAGAACCGCTAC
AGCTTTTACAGCACCTGCAGTGGTCAGAAGGCCATAAAGAAGTGCCCTGTGCGCCCGCCCTCTTGTGCC
TCCAAGCAGGACCCTGTGTATATCCCGCCCATCTCCTGCAACAAGGACCTGTCCTTTGGCCACTCTAGG
GCCAGCTCCAAGATCTGCAGTGAGGACATCGAGTGCAGTGGGCTGACCATCCCCAAGGCTGTGCAGTAC
CTGAGCTCCCAGGATGAGAAGTACCAGGCCATTGGGGCCTATTACATCCAGCATACCTGCTTCCAGGAT
GAATCTGCCAAGCAACAGGTCTATCAGCTGGGAGGCATCTGCAAGCTGGTGGACCTCCTCCGCAGCCCC
AACCAGAACGTCCAGCAGGCCGCGGCAGGGGCCCTGCGCAACCTGGTGTTCAGGAGCACCACCAACAAG
CTGGAGACCCGGAGGCAGAATGGGATCCGCGAGGCAGTCAGCCTCCTGAGGAGAACCGGGAACGCCGAG
ATCCAGAAGCAGCTGACTGGGCTGCTCTGGAACCTGTCTTCCACTGACGAGCTGAAGGAGGAACTCATT
GCCGACGCCCTGCCTGTTCTGGCCGACCGCGTCATCATTCCCTTCTCTGGCTGGTGCGATGGCAATAGC
AACATGTCCCGGGAAGTGGTGGACCCTGAGGTCTTCTTCAATGCCACAGGCTGCTTGAGAAAGAGACTG
GGCATGCGGGAGCTTCTGGCTCTTGTTCCGCAAAGGGCCACTAGTAGCAGGGTGAACCTGAGCTCGGCC
GATGCAGGCCGCCAGACCATGCGTAACTACTCAGGGCTCATTGATTCCCTCATGGCCTATGTCCAGAAC
TGTGTAGCGGCCAGCCGCTGTGACGACAAGTCTGTGGAAAACTGCATGTGTGTTCTGCACAACCTCTCC
TACCGCCTGGACGCCGAGGTGCCCACCCGCTACCGCCAGCTGGAGTATAACGCCCGCAACGCCTACACC
GAGAAGTCCTCCACTGGCTGCTTCAGCAACAAGAGCGACAAGATGATGAACAACAACTATGACTGCCCC
CTGCCTGAGGAAGAGACCAACCCCAAGGGCAGCGGCTGGTTGTACCATTCAGATGCCATCCGCACCTAC
CTGAACCTCATGGGCAAGAGCAAGAAAGATGCTACCCTGGAGGCCTGTGCTGGTGCCCTGCAGAACCTG
ACAGCCAGCAAGGGGCTGATGTCCAGTGGCATGAGCCAGTTGATTGGGCTGAAGGAAAAGGGCCTGCCA
CAAATTGCCCGCCTCCTGCAATCTGGCAACTCTGATGTGGTGCGGTCCGGAGCCTCCCTCCTGAGCAAC
ATGTCCCGCCACCCTCTGCTGCACAGAGTGATGGGGAACCAGGTGTTCCCGGAGGTGACCAGGCTCCTC
ACCAGCCACACTGGCAATACCAGCAACTCCGAAGACATCTTGTCCTCGGCCTGCTACACTGTGAGGAAC
CTGATGGCCTCGCAGCCACAACTGGCCAAGCAGTACTTCTCCAGCAGCATGCTCAACAACATCATCAAC
CTGTGCCGAAGCAGTGCCTCACCCAAGGCCGCAGAAGCTGCCCGGCTTCTCCTGTCTGACATGTGGTCC
AGCAAGGAACTGCAGGGTGTCCTCAGACAGCAAGGTTTCGATAGGAACATGCTGGGAACCTTAGCTGGG
GCCAACAGCCTCAGGAACTTCACCTCCCGATTCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_000299
Insert Size 2244 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000299.2
RefSeq Size 5447 bp
RefSeq ORF 2244 bp
Locus ID 5317
UniProt ID Q13835
Protein Families Druggable Genome
MW 82.9 kDa
Gene Summary This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Transcript Variant: This variant (1b) represents the longer transcript variant and encodes the longer protein (isoform 1b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...