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PDE6 alpha (PDE6A) (NM_000440) Human Untagged Clone
CAT＃: SC300070
PDE6A (untagged)-Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A)
CNY 6,304.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGPR-A; PDEA; RP43 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000440 edited
TTGCAGACTGCAAAACTGCCATTGGAAGGCCTCCGTCCCAGTCCTTCTACAGAGTAGCCA GTGGGATTCCCAGCCATGGGCGAGGTGACAGCAGAGGAGGTGGAGAAGTTCCTGGACTCG AATATTGGCTTTGCCAAACAGTACTACAACCTCCACTACCGGGCCAAGCTCATCTCCGAC CTCCTTGGGGCCAAGGAGGCTGCCGTGGACTTCAGCAACTACCACTCCCCGAGCAGCATG GAGGAGAGCGAAATCATCTTTGATCTCCTGCGGGACTTTCAGGAGAATTTACAGACAGAG AAATGCATCTTCAATGTCATGAAGAAGCTGTGCTTCCTCCTGCAGGCAGACCGCATGAGC CTGTTCATGTACCGGACCCGCAATGGCATCGCAGAGCTGGCCACCAGGCTTTTCAATGTC CACAAGGATGCTGTCCTCGAGGACTGCCTGGTGATGCCCGACCAAGAGATCGTCTTCCCT TTGGACATGGGCATCGTGGGCCATGTCGCACACTCTAAGAAGATTGCTAACGTCCCCAAC ACAGAGGAGGATGAGCATTTCTGTGACTTTGTGGACATCCTCACAGAGTACAAGACCAAG AACATCTTGGCTTCCCCCATAATGAATGGGAAGGATGTGGTGGCCATAATCATGGCTGTG AATAAAGTGGATGGATCCCACTTCACCAAGAGAGATGAAGAGATTCTTCTCAAGTACCTC AATTTTGCAAATCTAATCATGAAGGTGTACCACCTGAGTTACCTGCACAACTGTGAAACT CGACGTGGCCAGATACTGCTGTGGTCTGGGAGCAAAGTCTTTGAAGAACTTACGGACATC GAACGACAGTTCCACAAAGCCCTGTACACAGTCCGTGCTTTCCTCAACTGTGACAGATAC TCTGTGGGTCTCTTAGACATGACCAAGCAGAAGGAATTTTTTGATGTGTGGCCGGTTCTG ATGGGTGAAGTTCCACCTTACTCTGGTCCCAGGACTCCGGATGGAAGAGAAATTAACTTT TACAAGGTCATTGACTACATCCTGCATGGCAAAGAGGACATCAAAGTCATCCCGAATCCA CCTCCTGACCATTGGGCTTTAGTAAGCGGTCTCCCAGCTTATGTTGCCCAGAATGGCCTG ATTTGCAACATCATGAACGCGCCTGCGGAGGACTTTTTTGCATTTCAGAAAGAACCTCTG GATGAGTCTGGATGGATGATTAAAAATGTGCTTTCAATGCCGATTGTGAACAAGAAGGAA GAAATTGTTGGAGTGGCCACATTTTACAATCGTAAAGATGGGAAGCCCTTTGATGAAATG GATGAGACGCTCATGGAGTCTTTGACTCAATTTCTGGGCTGGTCTGTCTTAAATCCTGAC ACCTATGAGTCAATGAATAAACTTGAAAATAGGAAGGATATTTTCCAGGACATAGTAAAA TATCATGTGAAGTGTGACAATGAAGAAATTCAGAAAATCTTGAAAACCAGAGAGGTGTAT GGGAAGGAGCCATGGGAGTGTGAGGAAGAGGAGCTGGCTGAGATCCTGCAAGCGGAGCTG CCAGATGCAGATAAATACGAAATTAATAAATTTCACTTCAGTGACTTACCCCTAACAGAA CTGGAGCTGGTAAAATGTGGAATACAGATGTATTATGAGCTCAAAGTGGTGGATAAATTT CACATTCCACAAGAGGCCCTGGTGCGGTTCATGTACTCCCTGAGTAAGGGCTACCGCAAG ATCACCTACCACAACTGGCGGCACGGCTTCAACGTGGGGCAGACCATGTTCTCCCTGCTG GTGACGGGAAAGCTGAAGCGCTACTTCACGGACCTAGAGGCCTTGGCCATGGTCACTGCT GCTTTCTGCCATGACATTGACCACAGAGGCACCAATAACCTCTACCAGATGAAATCCCAG AACCCACTGGCCAAGCTCCATGGGTCCTCTATCTTGGAAAGACACCACTTGGAGTTTGGC AAAACACTGCTCAGAGACGAGAGCCTGAATATCTTTCAAAACCTCAATCGTCGACAGCAT GAGCATGCCATCCACATGATGGACATTGCAATCATTGCCACAGACCTCGCCCTGTATTTC AAGAAGAGGACGATGTTCCAAAAGATCGTGGATCAGTCTAAGACATATGAGAGTGAACAG GAGTGGACACAGTACATGATGCTGGAGCAGACACGGAAGGAAATCGTTATGGCCATGATG ATGACCGCCTGTGATCTCTCAGCCATCACCAAACCCTGGGAGGTGCAGAGCCAGGTAGCT CTGCTGGTGGCTGCTGAATTCTGGGAACAAGGTGACCTGGAGCGCACGGTGCTGCAACAG AATCCCATTCCCATGATGGACAGAAACAAAGCAGATGAACTCCCTAAGCTTCAAGTCGGC TTCATTGACTTTGTTTGCACCTTCGTCTACAAGGAATTCTCCCGTTTCCACGAGGAGATC ACCCCAATGTTGGACGGGATCACCAACAATCGCAAGGAGTGGAAGGCGCTTGCTGATGAG TACGATGCCAAGATGAAGGTGCAGGAGGAGAAGAAGCAGAAACAGCAGTCGGCCAAGTCA GCAGCCGCAGGAAATCAGCCGGGGGGAAACCCCAGCCCAGGGGGTGCAACTACATCCAAG TCCTGCTGCATCCAGTAACACCACTGGGGATGTGCTGGCTGGACGGCACCACCCTTTCCT GGGAAGAGATGACTCAAGCCAGTGGAAGACCACACACCTTGAGAAGTAGAAGAGTCATAG GATTTGAAAGCTGTTAGAGAATTTAGCTTCCAGGACTGTTCAATCTTTTGGCTTCCCTGG GCCACATTGGAAGAATTGTCTTGGGTCACACATAAAATACAGTAACACTAATGATAGCTG TTGAACTTAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_000440 unedited
AGGGGAGGGGGGNNNGGGGGGGTCATATTTGTATACGACTCACTATAGGCGGCCGCGAAT TCGGCACGAGGTTGCAGACTGCAAAACTGCCATTGGAAGGCCTCCGTCCCAGTCCTTCTA CAGAGTAGCCAGTGGGATTCCCAGCCATGGGCGAGGTGACAGCAGAGGAGGTGGAGAAGT TCCTGGACTCGAATATTGGCTTTGCCAAACAGTACTACAACCTCCACTACCGGGCCAAGC TCATCTCCGACCTCCTTGGGGCCAAGGAGGCTGCCGTGGACTTCAGCAACTACCACTCCC CGAGCAGCATGGAGGAGAGCGAAATCATCTTTGATCTCCTGCGGGACTTTCAGGAGAATT TACAGACAGAGAAATGCATCTTCAATGTCATGAAGAAGCTGTGCTTCCTCCTGCAGGCAG ACCGCATGAGCCTGTTCATGTACCGGACCCGCAATGGCATCGCAGAGCTGGCCACCAGGC TTTTCAATGTCCACAAGGATGCTGTCCTCGAGGACTGCCTGGTGATGCCCGACCAAGAGA TCGTCTTCCCTTTGGACATGGGCATCGTGGGCCATGTCGCACACTCTAAGAAGATTGCTA ACGTCCCCAACACAGAGGAGGATGAGCATTTCTGTGACTTTGTGGACATCCTCACAGAGT ACAAGACCAAGAACATCTTGGCTTCCCCCATAATGAATGGGAAGGATGTGGTGGCCATAA TCATGGCTGTGAATAAAGTGGATGGATCCCACTTCACCAAGAGAGATGAAGAGATTCTTC TCAAGTACCTCAATTNTGCAAATCTAATCATGAAGGTGTACCACCTGAGTTACCTGCANC ACTGTGAAACTCGACGTGGCCAGATACTGCTGTGGTCTGGGAG >OriGene 3' read for NM_000440 unedited
NGGGTTTTGNNNNNNNNCCCGCCTTGGNNGATGGCACTTNCCAGGNCCAGNAAAGCACTG GGGNAGGGTCACAGGGNTGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCG CAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAGTTCAACAGCTATCATTAGTGTTA CTGTATTTTATGTGTGACCCAAGACAATTCTTCCAATGTGGCCCAGGGAAGCCAAAAGAT TGAACAGTCCTGGAAGCTAAATTCTCTAACAGCTTTCAAATCCTATGACTCTTCTACTTC TCAAGGTGTGTGGTCTTCCACTGGCTTGAGTCATCTCTTCCCAGGAAAGGGTGGTGCCGT CCAGCCAGCACATCCCCAGTGGTGTTACTGGATGCAGCAGGACTTGGATGTAGTTGCACC CCCTGGGCTGGGGTTTCCCCCCGGCTGATTTCCTGCGGCTGCTGACTTGGCCGACTGCTG TTTCTGCTTCTTCTCCTCCTGCACCTTCATCTTGGCATCGTACTCATCAGCAAGCGCCTT CCACTCCTTGCGATTGTTGGTGATCCCGTCCAACATTGGGGTGATCTCCTCGTGGAAACG GGAGAATTCCTTGTAGACGAAGGTGCAAACAAAGTCAATGAAGCCGACTTGAAGCTTAGG GAGTTCATCTGCTTTGTTTCTGTCCATCATGGGAATGGGATTCTGTTGCAGCACCGTGCG CTCCANGTCACCTTGTTCCCAGATTTCAGCAGCCACCAGCAGAGCTACCTGGCTCTGCAC CTCCCAGGNTTTGGTGATGGCTGAGAGATCACAGGCGGTCATCATCATGGCCATAACGAT TTCCTTCCGTGTCTGCTNCAGCATCATGTACTGTGTCCACTCCTGGTCACTCTCATATGA |
| Restriction Sites | Please inquire |
| ACCN | NM_000440 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000440.1, NP_000431.1 |
| RefSeq Size | 2944 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 5145 |
| UniProt ID | P16499 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207783 | PDE6A (Myc-DDK-tagged)-Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A) |
CNY 6,296.00 |
|
| RC207783L3 | Lenti ORF clone of Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207783L4 | Lenti ORF clone of Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), mGFP tagged |
CNY 5,890.00 |
|
| RG207783 | PDE6A (tGFP-tagged) - Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A) |
CNY 7,896.00 |
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