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PDE6 alpha (PDE6A) (NM_000440) Human Tagged ORF Clone
CAT＃: RC207783
PDE6A (Myc-DDK-tagged)-Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A)
ORF Plasmid: tGFP
"NM_000440" in other vectors (4)
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CNY 6,296.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGPR-A; PDEA; RP43 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207783 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGGTGACAGCAGAGGAGGTGGAGAAGTTCCTGGACTCGAATATTGGCTTTGCCAAACAGTACT ACAACCTCCACTACCGGGCCAAGCTCATCTCCGACCTCCTTGGGGCCAAGGAGGCTGCCGTGGACTTCAG CAACTACCACTCCCCGAGCAGCATGGAGGAGAGCGAAATCATCTTTGATCTCCTGCGGGACTTTCAGGAG AATTTACAGACAGAGAAATGCATCTTCAATGTCATGAAGAAGCTGTGCTTCCTCCTGCAGGCAGACCGCA TGAGCCTGTTCATGTACCGGACCCGCAATGGCATCGCAGAGCTGGCCACCAGGCTTTTCAATGTCCACAA GGATGCTGTCCTCGAGGACTGCCTGGTGATGCCCGACCAAGAGATCGTCTTCCCTTTGGACATGGGCATC GTGGGCCATGTCGCACACTCTAAGAAGATTGCTAACGTCCCCAACACAGAGGAGGATGAGCATTTCTGTG ACTTTGTGGACATCCTCACAGAGTACAAGACCAAGAACATCTTGGCTTCCCCCATAATGAATGGGAAGGA TGTGGTGGCCATAATCATGGCTGTGAATAAAGTGGATGGATCCCACTTCACCAAGAGAGATGAAGAGATT CTTCTCAAGTACCTCAATTTTGCAAATCTAATCATGAAGGTGTACCACCTGAGTTACCTGCACAACTGTG AAACTCGACGTGGCCAGATACTGCTGTGGTCTGGGAGCAAAGTCTTTGAAGAACTTACGGACATCGAACG ACAGTTCCACAAAGCCCTGTACACAGTCCGTGCTTTCCTCAACTGTGACAGATACTCTGTGGGTCTCTTA GACATGACCAAGCAGAAGGAATTTTTTGATGTGTGGCCGGTTCTGATGGGTGAAGTTCCACCTTACTCTG GTCCCAGGACTCCGGATGGAAGAGAAATTAACTTTTACAAGGTCATTGACTACATCCTGCATGGCAAAGA GGACATCAAAGTCATCCCGAATCCACCTCCTGACCATTGGGCTTTAGTAAGCGGTCTCCCAGCTTATGTT GCCCAGAATGGCCTGATTTGCAACATCATGAATGCGCCTGCGGAGGACTTTTTTGCATTTCAGAAAGAAC CTCTGGATGAGTCTGGATGGATGATTAAAAATGTGCTTTCAATGCCGATTGTGAACAAGAAGGAAGAAAT TGTTGGAGTGGCCACATTTTACAATCGTAAAGATGGGAAGCCCTTTGATGAAATGGATGAGACGCTCATG GAGTCTTTGACTCAATTTCTGGGCTGGTCTGTCTTAAATCCTGACACCTATGAGTCAATGAATAAACTTG AAAATAGGAAGGATATTTTCCAGGACATAGTAAAATATCATGTGAAGTGTGACAATGAAGAAATTCAGAA AATCTTGAAAACCAGAGAGGTGTATGGGAAGGAGCCATGGGAGTGTGAGGAAGAGGAGCTGGCTGAGATC CTGCAAGCGGAGCTGCCAGATGCAGATAAATACGAAATTAATAAATTTCACTTCAGTGACTTACCCCTAA CAGAACTGGAGCTGGTAAAATGTGGAATACAGATGTATTATGAGCTCAAAGTGGTGGATAAATTTCACAT TCCACAAGAGGCCCTGGTGCGGTTCATGTACTCCCTGAGTAAGGGCTACCGCAAGATCACCTACCACAAC TGGCGGCACGGCTTCAACGTGGGGCAGACCATGTTCTCCCTGCTGGTGACGGGAAAGCTGAAGCGCTACT TCACGGACCTAGAGGCCTTGGCCATGGTCACTGCTGCTTTCTGCCATGACATTGACCACAGAGGCACCAA TAACCTCTACCAGATGAAATCCCAGAACCCACTGGCCAAGCTCCATGGGTCCTCTATCTTGGAAAGACAC CACTTGGAGTTTGGCAAAACACTGCTCAGAGACGAGAGCCTGAATATCTTTCAAAACCTCAATCGTCGAC AGCATGAGCATGCCATCCACATGATGGACATTGCAATCATTGCCACAGACCTCGCCCTGTATTTCAAGAA GAGGACGATGTTCCAAAAGATCGTGGATCAGTCTAAGACATATGAGAGTGAACAGGAGTGGACACAGTAC ATGATGCTGGAGCAGACACGGAAGGAAATCGTTATGGCCATGATGATGACCGCCTGTGATCTCTCAGCCA TCACCAAACCCTGGGAGGTGCAGAGCCAGGTAGCTCTGCTGGTGGCTGCTGAATTCTGGGAACAAGGTGA CCTGGAGCGCACGGTGCTGCAACAGAATCCCATTCCCATGATGGACAGAAACAAAGCAGATGAACTCCCT AAGCTTCAAGTCGGCTTCATTGACTTTGTTTGCACCTTCGTCTACAAGGAATTCTCCCGTTTCCACGAGG AGATCACCCCAATGTTGGACGGGATCACCAACAATCGCAAGGAGTGGAAGGCGCTTGCTGATGAGTACGA TGCCAAGATGAAGGTGCAGGAGGAGAAGAAGCAGAAACAGCAGTCGGCCAAGTCAGCAGCCGCAGGAAAT CAGCCGGGGGGAAACCCCAGCCCAGGGGGTGCAACTACATCCAAGTCCTGCTGCATCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207783 protein sequence
Red=Cloning site Green=Tags(s) MGEVTAEEVEKFLDSNIGFAKQYYNLHYRAKLISDLLGAKEAAVDFSNYHSPSSMEESEIIFDLLRDFQE NLQTEKCIFNVMKKLCFLLQADRMSLFMYRTRNGIAELATRLFNVHKDAVLEDCLVMPDQEIVFPLDMGI VGHVAHSKKIANVPNTEEDEHFCDFVDILTEYKTKNILASPIMNGKDVVAIIMAVNKVDGSHFTKRDEEI LLKYLNFANLIMKVYHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLL DMTKQKEFFDVWPVLMGEVPPYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPAYV AQNGLICNIMNAPAEDFFAFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEMDETLM ESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEI LQAELPDADKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRKITYHN WRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERH HLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESEQEWTQY MMLEQTRKEIVMAMMMTACDLSAITKPWEVQSQVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELP KLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYDAKMKVQEEKKQKQQSAKSAAAGN QPGGNPSPGGATTSKSCCIQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000440 |
| ORF Size | 2580 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000440.3 |
| RefSeq Size | 5659 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 5145 |
| UniProt ID | P16499 |
| Protein Families | Druggable Genome |
| MW | 99.5 kDa |
| Gene Summary | This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Splicing-accessible coding 3\'UTRs control protein stability and interaction networks
,Preussner, M;Gao, Q;Morrison, E;Herdt, O;Finkernagel, F;Schumann, M;Krause, E;Freund, C;Chen, W;Heyd, F;,
Genome Biol.
,PubMed ID 32727563
[PDE6 alpha]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207783L3 | Lenti ORF clone of Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207783L4 | Lenti ORF clone of Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), mGFP tagged |
CNY 5,890.00 |
|
| RG207783 | PDE6A (tGFP-tagged) - Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A) |
CNY 7,896.00 |
|
| SC300070 | PDE6A (untagged)-Human phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A) |
CNY 6,304.00 |
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