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LCMT1 (NM_001032391) Human Untagged Clone
CAT＃: SC302654
LCMT1 (untagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2
CNY 6,270.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CGI-68; LCMT; PPMT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC302654 representing NM_001032391.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCACTAGGCAGAGGGAATCCTCTATCACCTCCTGCTGTTCCACCTCGAGCTGCGACGCAGACGAC GAGGGCGTGCGCGGCACCTGCGAAGATGCTTCCCTGTGCAAGAGGTTTGCAGTAAGCATTGGCTACTGG CATGACCCTTACATACAGCACTTTGTGAGACTGTCTAAAGAGAGGAAAGCCCCTGAAATCAACAGAGGA TATTTTGCTCGAGTCCATGGTGTCAGTCAGCTTATAAAGGCATTTCTACGGAAGACAGAATGTCATTGT CAAATTGTCAACCTTGGGGCAGGCATGGATACCACCTTCTGGAGATTAAAGGATGAAGATCTTCTCCCA AGTAAATATTTTGAGGTTGACTTTCCAATGATTGTCACGAGAAAGCTGCACAGTATCAAATTGCCAACA CTCCTGATAGCTGAATGTGTGCTGGTTTACATGACTCCAGAGCAGTCCGCAAACCTCCTGAAGTGGGCA GCCAACAGTTTTGAGAGAGCCATGTTCATAAACTACGAACAGGTGAACATGGGTGATCGGTTTGGGCAG ATCATGATTGAAAACCTGCGGAGACGCCAGTGTGACCTGGCGGGAGTGGAGACCTGCAAGTCATTAGAG TCACAGAAAGAACGGCTCCTGTCGAATGGGTGGGAAACAGCATCGGCCGTCGACATGATGGAGTTGTAC AACAGGTTACCTCGAGCTGAAGTGAGCAGGATAGAATCACTTGAATTCCTGGATGAAATGGAGCTGCTG GAGCAGCTCATGCGGCATTACTGCCTTTGCTGGGCAACCAAAGGAGGAAATGAGCTTGGGCTGAAGGAG ATAACTTATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001032391 |
| Insert Size | 840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001032391.1 |
| RefSeq Size | 1209 bp |
| RefSeq ORF | 840 bp |
| Locus ID | 51451 |
| UniProt ID | Q9UIC8 |
| Protein Pathways | Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism |
| MW | 32.2 kDa |
| Gene Summary | LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) lacks two alternate internal exons that encode an in-frame segment, compared to variant 1. The resulting isoform (b) is shorter than isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222891 | LCMT1 (Myc-DDK-tagged)-Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2 |
CNY 2,400.00 |
|
| RC222891L1 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC222891L2 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC222891L3 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222891L4 | Lenti ORF clone of Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG222891 | LCMT1 (tGFP-tagged) - Human leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 2 |
CNY 4,370.00 |
