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DENND3 (NM_014957) Human Untagged Clone
CAT＃: SC304188
DENND3 (untagged)-Human DENN/MADD domain containing 3 (DENND3)
CNY 23,090.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_014957 edited
ATGCGCTCCTTGAGAAAGAAGAGAGAGAAGCCCAGACCAGAGCAGTGGAAGGGCCTCCCG GGGCCCCCCAGAGCGCCAGAGCCTGAGGATGTCGCCGTCCCGGGCGGCGTGGACCTCCTC ACCCTGCCGCAGCTGTGCTTCCCAGGGGGTGTGTGCGTGGCCACTGAACCTAAGGAGGAT TGCGTCCACTTCCTGGTGCTGACCGATGTCTGCGGGAATAGGACCTATGGCGTGGTGGCC CAGTACTACCGGCCCCTGCATGATGAGTACTGTTTCTACAATGGCAAAACGCACCGGGAG TGTCCTGGCTGCTTCGTGCCCTTCGCGGTGTGCGTGGTCTCCAGGTTTCCCTATTACAAC TCCCTCAAGGACTGCCTTTCCTGTTTATTGGCTCTTCTGAAGCCCTGTAAAGATTTTGAA GTGGACAGTCATATAAAAGATTTCGCTGCGAAGCTGTCTTTAATACCCAGCCCGCCACCT GGACCGCTCCATTTGGTATTTAACATGAAGTCGCTCCAGATTGTGTTACCTGCCCGAGCA GACCCCGAAAGCCCCATCCTGGACCTGGACCTTCACCTGCCCTTGCTGTGCTTCAGGCCT GAGAAGGTGCTACAGATCCTGACATGCATCCTGACGGAACAGCGGATCGTCTTCTTCTCC TCGGACTGGGCTCTGCTGACGCTGGTCACTGAGTGCTTCATGGCCTACCTGTATCCGCTG CAGTGGCAGCACCCCTTCGTGCCCATCCTGTCGGACCAGATGCTGGATTTCGTCATGGCC CCCACGTCCTTCCTGATGGGCTGCCATCTCGACCACTTCGAAGAAGTCAGCAAGGAAGCC GACGGTTTAGTTCTGATAAATATTGATCATGGGAGCATCACCTACTCCAAGTCCACGGAC GATAACGTGGACATTCCTGATGTCCCCCTCCTGGCAGCCCAGACGTTTATTCAGAGGGTG CAGAGCCTCCAGCTCCACCATGAGCTGCACGCCGCCCACCTCCTCTCCAGCACAGACCTG AAGGAGGGCCGAGCCCACCGGCGGTCCTGGCAGCAGAAACTCAACTGCCAGATACAGCAG ACCACCCTGCAGCTGCTCGTGAGCATCTTCAGGGATGTAAAGAATCATTTAAACTATGAA CACAGAGTCTTTAATAGTGAAGAATTTCTCAAAACCAGGGCTCCAGGGGACCATCAGTTT TATAAGCAGGTCTTAGACACCTACATGTTCCATTCTTTTCTTAAAGCCCGGCTCAATAGG AGGATGGACGCCTTTGCTCAGATGGACCTCGACACCCAGTCGGAGGAGGACAGAATAAAT GGAATGCTTCTAAGTCCAAGGAGACCGACCGTTGAGAAAAGAGCCTCCCGGAAGTCCTCG CACCTGCATGTCACCCACAGGCGCATGGTGGTCAGCATGCCCAACCTGCAGGACATTGCC ATGCCTGAGCTGGCACCCAGGAACTCCTCGCTCCGGCTGACGGACACCGCAGGCTGTAGG GGCAGCAGCGCAGTTCTGAATGTCACGCCGAAGTCCCCGTATACATTCAAGATTCCCGAA ATCCACTTTCCGCTGGAGAGCAAGTGCGTGCAGGCATACCATGCCCACTTTGTCTCCATG CTGAGCGAGGCCATGTGCTTTCTGGCCCCCGATAACTCTCTGCTCCTGGCCCGCTATTTG TACCTCCGAGGGCTCGTTTATCTGATGCAGGGACAGCTGCTGAACGCCCTCTTGGACTTC CAGAATCTGTATAAAACAGACATACGGATCTTTCCCACTGATTTGGTGAAGAGGACGGTG GAATCCATGTCTGCCCCTGAGTGGGAGGGGGCTGAGCAGGCGCCGGAGCTGATGAGGCTC ATCAGCGAGATCCTGGACAAGCCGCACGAGGCCTCGAAGCTGGACGACCACGTGAAGAAG TTCAAGCTGCCCAAGAAGCACATGCAGCTGGGCGACTTCATGAAGCGGGTCCAGGAGTCA GGGATCGTGAAGGACGCCAGCATCATACACCGGCTGTTCGAGGCCTTGACTGTAGGACAG GAGAAACAAATCGACCCAGAAACATTCAAAGATTTCTACAACTGCTGGAAGGAGACGGAA GCAGAAGCCCAGGAGGTCAGTCTGCCGTGGCTGGTGATGGAACACCTGGATAAAAACGAG TGTGTGTGTAAGTTGTCCAGCTCCGTCAAGACAAACCTAGGCGTTGGCAAGATCGCCATG ACCCAGAAGCGCCTGTTCCTCCTAACCGAAGGAAGGCCAGGCTACTTGGAGATTTCCACC TTCAGAAATATAGAGGAGGTCAGGAGAACCACTACTACATTTCTACTTCGGAGAATACCC ACTTTAAAAATCAGAGTGGCGTCCAAGAAAGAAGTCTTCGAAGCCAACCTGAAAACCGAG TGTGACCTTTGGCACCTGATGGTGAAGGAGATGTGGGCTGGGAAGAAGCTGGCCGATGAC CACAAGGACCCTCACTACGTCCAGCAGGCGCTGACCAACGTCTTGCTGATGGACGCCGTC GTGGGCACACTGCAGTCACCAGGCGCCATCTACGCTGCCTCCAAGTTATCCTACTTTGAT AAGATGAGTAACGAAATGCCCATGACGCTTCCGGAGACAACCCTGGAAACACTGAAGCAT AAAATCAACCCCTCGGCGGGGGAGGCGTTCCCACAAGCGGTGGACGTGCTGCTCTACACT CCAGGGCATCTTGACCCAGCCGAAAAAGTTGAAGATGCTCACCCCAAGTTATGGTGTGCT CTGAGCGAAGGCAAGGTGACCGTGTTCAATGCTTCTTCATGGACCATCCACCAGCACTCC TTTAAAGTGGGCACTGCAAAAGTGAACTGCATGGTGATGGCCGACCAGAACCAGGTGTGG GTTGGCTCGGAAGACTCCGTCATCTACATCATCAACGTCCACAGCATGTCCTGCAACAAG CAGCTCACAGCCCACTGCTCCAGTGTCACGGATTTGATTGTGCAGGACGGACAGGAGGCA CCCAGCAACGTGTACTCGTGCAGCATGGACGGCATGGTGCTGGTGTGGAATGTGAGCACA CTGCAGGTGACCAGCCGCTTCCAGCTGCCGCGAGGTGGCCTGACGTCCATCAGACTGCAC GGCGGCCGCCTGTGGTGCTGCACAGGTAACAGCATCATGGTCATGAAAATGAATGGATCC CTCCATCAAGAATTGAAGATTGAGGAGAACTTCAAAGACACCAGTACCTCCTTCCTGGCC TTCCAGCTCCTTCCTGAGGAGGAGCAGCTGTGGGCGGCCTGTGCAGGACGCAGCGAGGTT TACATCTGGAGCCTGAAGGACCTGGCCCAGCCCCCGCAGAGGGTGCCCCTCGAGGACTGC TCTGAGATCAACTGCATGATCCGGGTGAAGAAGCAGGTCTGGGTGGGCAGCCGAGGGCTG GGGCAGGGAACACCCAAGGGGAAAATCTACGTGATTGACGCCGAGAGGAAGACCGTGGAG AAGGAGCTGGTGGCGCACATGGACACCGTGAGGACGCTGTGCTCGGCTGAGGACAGATAC GTGCTGAGTGGGTCGGGCAGGGAGGAGGGGAAAGTCGCCATTTGGAAAGGCGAATAA |
| Restriction Sites | Please inquire |
| ACCN | NM_014957 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014957.2, NP_055772.2 |
| RefSeq Size | 5458 bp |
| RefSeq ORF | 3597 bp |
| Locus ID | 22898 |
| UniProt ID | A2RUS2 |
| Gene Summary | Guanine nucleotide exchange factor (GEF) activating RAB12. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (PubMed:20937701). Regulates autophagy in response to starvation through RAB12 activation. Starvation leads to ULK1/2-dependent phosphorylation of Ser-472 and Ser-490, which in turn allows recruitment of 14-3-3 adapter proteins and leads to up-regulation of GEF activity towards RAB12 (By similarity). Also plays a role in protein transport from recycling endosomes to lysosomes, regulating, for instance, the degradation of the transferrin receptor and of the amino acid transporter PAT4 (PubMed:20937701). Starvation also induces phosphorylation at Tyr-858, which leads to up-regulated GEF activity and initiates autophagy (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215141 | DENND3 (Myc-DDK-tagged)-Human DENN/MADD domain containing 3 (DENND3) |
CNY 8,344.00 |
|
| RC215141L3 | Lenti ORF clone of Human DENN/MADD domain containing 3 (DENND3), Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC215141L4 | Lenti ORF clone of Human DENN/MADD domain containing 3 (DENND3), mGFP tagged |
CNY 11,500.00 |
|
| RG215141 | DENND3 (tGFP-tagged) - Human DENN/MADD domain containing 3 (DENND3) |
CNY 9,944.00 |
