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LIMCH1 (NM_014988) Human Untagged Clone
CAT＃: SC304197
LIMCH1 (untagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1
CNY 7,552.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LIMCH1A; LMO7B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304197 representing NM_014988.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTTGTCCCGCTCTCGGTCTGGAAGCTCTTCAGCCCCTGCAGCCCGAGCCGCCCCCCGAGCCCGCC TTCTCCGAGGCGCAGAAGTGGATTGAGCAAGTAACTGGCAGAAGTTTTGGTGATAAAGATTTTCGGACA GGTTTAGAAAATGGAATCCTCCTCTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATC AATAGATTGCCTACCCCCATTGCAGGACTGGACAATATTATCTTATTCTTGAGAGGTTGTAAAGAGCTC GGCCTTAAAGAATCTCAACTTTTTGACCCGAGTGACCTCCAGGATACATCCAACAGAGTAACAGTCAAG AGCCTTGATTATAGTAGGAAGCTGAAAAATGTATTAGTTACCATTTACTGGCTGGGAAAAGCAGCAAAC AGCTGCACATCCTACAGCGGAACGACACTAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATGCGA AAGGACACTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATCGACTGCTGGGAT TCCGAGCGCAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTCCTTCGACAGCCTGGATTCC TTTGGCTCTCGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGAGGA AGCGACTCTGAATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCACGG CGGACTTCCCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCAG ACGGCCTACGTCCCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGT ACCGCCACCTCCCCGCTGGGTGGGGAGAGGCCCTTCAGATACGGTCCGAGAACTCCTGTGTCTGATGAC GCAGAGAGCACCAGCATGTTTGACATGCGGTGTGAGGAGGAGGCCGCGGTGCAGCCGCACAGCAGGGCC CGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAGGGAAGAGGACGACAAATGGCAAGATGACCTG GCTCGTTGGAAGAGTCGTAGAAGAAGTGTTTCTCAGGACTTAATCAAGAAAGAGGAAGAAAGGAAAAAA ATGGAGAAGTTACTGGCTGGAGAAGATGGGACAAGTGAACGAAGGAAAAGCATCAAAACCTACAGAGAA ATTGTTCAAGAAAAAGAGCGGAGAGAGAGAGAGCTGCATGAAGCATATAAGAACGCTCGGTCCCAGGAG GAGGCAGAGGGGATCCTTCAACAGTACATTGAGAGGTTCACCATCAGTGAGGCTGTTCTCGAACGCTTG GAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACAGAGCCAAATTTATCCTCCTTCCTGAATGACCCC AATCCCATGAAATACCTGCGGCAACAGTCACTGCCTCCACCCAAATTCACTGCCACTGTTGAAACCACC ATTGCTCGTGCCAGTGTTCTGGATACCAGCATGTCAGCAGGCAGTGGGTCTCCAAGCAAAACTGTCACT CCCAAAGCAGTGCCTATGCTGACACCCAAGCCTTACTCCCAGCCCAAAAATTCTCAAGATGTTCTGAAG ACCTTTAAGGTAGACGGGAAAGTCAGTGTGAATGGAGAGACGGTTCATAGAGAGGAGGAGAAGGAAAGA GAGTGTCCCACGGTGGCACCTGCCCACTCCTTAACCAAATCCCAGATGTTTGAAGGTGTGGCCAGAGTG CACGGGTCTCCACTGGAGCTGAAACAAGACAACGGTAGCATCGAGATCAACATAAAGAAGCCAAACTCT GTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACGGAACCGAATAGTCAAGAGGACAAGAATGATGGT GGAAAATCAAGAAAAGGGAATATAGAACTTGCCTCATCAGAACCACAGCATTTTACAACAACTGTGACT CGATGCAGCCCGACCGTGGCCTTTGTGGAATTTCCCTCCAGCCCCCAGCTGAAGAATGATGTGTCGGAA GAAAAAGACCAGAAGAAACCAGAAAATGAAATGAGTGGAAAGGTGGAGTTGGTGCTGTCACAAAAGGTG GTAAAGCCAAAATCTCCAGAACCCGAAGCAACGCTGACATTTCCATTTCTGGACAAAATGCCTGAAGCC AACCAACTACATTTGCCAAATCTCAATTCTCAAGTGGATTCTCCAAGCAGTGAGAAGTCACCTGTTACG ACACCTCAGTTTAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCAGGCGACAGGAAAAATGGCAACAG GAACAGGAACGTTTGCTCCAGGAGAGATACCAGAAGGAGCAGGACAAGCTGAAAGAAGAGTGGGAAAAG GCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGATACTATGAGGAGGAGCGTAAGATAATTGAAGACACT GTGGTTCCATTTACTGTTTCTTCAAGTTCCGCTGACCAGCTGTCTACCTCTTCCTCCATGACTGAAGGC AGTGGGACAATGAATAAGATAGACCTGGGAAACTGTCAAGATGAAAAACAAGACAGAAGATGGAAGAAA TCATTCCAGGGAGATGACAGTGACTTATTGCTGAAGACTAGGGAAAGTGATCGACTGGAGGAGAAGGGC AGCCTAACTGAAGGGGCCTTGGCTCATTCTGGGAACCCTGTATCAAAAGGAGTCCATGAAGACCATCAG CTGGATACCGAGGCTGGGGCCCCACACTGTGGAACAAACCCACAGCTTGCTCAGGATCCATCCCAGAAT CAGCAGACATCAAATCCAACGCACAGTTCAGAAGATGTGAAGCCAAAAACCCTCCCGCTGGATAAAAGC ATTAACCATCAGATCGAGTCTCCCAGTGAAAGGCGGAAGAAAAGTCCCCGAGAGCACTTCCAGGCTGGG CCTTTCTCTCCCTGTTCTCCCACCCCTCCCGGTCAGTCACCAAACAGGTCTATAAGTGGAAAGAAGCTG TGCTCTTCCTGTGGGCTTCCTTTGGGTAAAGGAGCTGCAATGATCATCGAGACCCTCAATCTCTATTTT CACATCCAGTGTTTCAGGTGTGGAATTTGTAAAGGCCAGCTTGGAGATGCAGTGAGTGGGACGGATGTT AGGATTCGAAATGGTCTCCTGAACTGTAATGATTGCTACATGCGATCCAGAAGTGCCGGGCAGCCTACA ACATTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_014988 |
| Insert Size | 3252 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014988.1 |
| RefSeq Size | 6184 bp |
| RefSeq ORF | 3252 bp |
| Locus ID | 22998 |
| UniProt ID | Q9UPQ0 |
| MW | 121.8 kDa |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform (a). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221099 | LIMCH1 (Myc-DDK-tagged)-Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1 |
CNY 7,544.00 |
|
| RC221099L3 | Lenti ORF clone of Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, Myc-DDK-tagged |
CNY 13,300.00 |
|
| RC221099L4 | Lenti ORF clone of Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1, mGFP tagged |
CNY 13,300.00 |
|
| RG221099 | LIMCH1 (tGFP-tagged) - Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 1 |
CNY 12,540.00 |
