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DENND1A (NM_020946) Human Untagged Clone
CAT＃: SC304873
DENND1A (untagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 1
CNY 7,032.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FAM31A; KIAA1608 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304873 representing NM_020946
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCAGGATCAAGCAGAATCCAGAGACCACATTTGAAGTATATGTTGAAGTGGCCTATCCCAGGA CAGGTGGCACTCTTTCAGATCCTGAGGTGCAGAGGCAATTCCCGGAGGACTACAGTGACCAGGAAGTTCT ACAGACTTTGACCAAGTTTTGTTTCCCCTTCTATGTGGACAGCCTCACAGTTAGCCAAGTTGGCCAGAAC TTCACATTCGTGCTCACTGACATTGACAGCAAACAGAGATTCGGGTTCTGCCGCTTATCTTCAGGAGCGA AGAGCTGCTTCTGTATCTTAAGCTATCTCCCCTGGTTCGAGGTATTTTATAAGCTGCTTAACATCCTGGC AGATTACACGACAAAAAGACAGGAAAATCAGTGGAATGAGCTTCTTGAAACTCTGCACAAACTTCCCATC CCTGACCCAGGAGTGTCTGTCCATCTCAGCGTGCATTCTTATTTTACTGTGCCTGATACCAGAGAACTTC CCAGCATACCTGAGAATAGAAATCTGACAGAATATTTTGTGGCTGTGGATGTTAACAACATGTTGCATCT GTACGCCAGTATGCTGTACGAACGCCGGATACTCATCATTTGCAGCAAACTCAGCACTCTGACTGCCTGC ATCCACGGGTCTGCGGCGATGCTCTACCCCATGTACTGGCAGCACGTGTACATCCCCGTGCTGCCGCCGC ATCTGCTGGACTACTGCTGTGCTCCCATGCCCTACCTCATAGGAATCCATTTAAGTTTAATGGAGAAAGT CAGAAACATGGCCCTGGATGATGTCGTGATCCTGAATGTGGACACCAACACCCTGGAAACCCCCTTCGAT GACCTCCAGAGCCTCCCAAACGACGTGATCTCTTCCCTGAAGAACAGGCTGAAAAAGGTCTCCACAACCA CTGGGGATGGTGTGGCCAGAGCGTTCCTCAAGGCCCAGGCTGCTTTCTTCGGTAGCTACCGAAACGCTCT GAAAATCGAGCCGGAGGAGCCGATCACTTTCTGTGAGGAAGCCTTCGTGTCCCACTACCGCTCCGGAGCC ATGAGGCAGTTCCTGCAGAACGCCACACAGCTGCAGCTCTTCAAGCAGTTTATTGATGGTCGATTAGATC TTCTCAATTCCGGCGAAGGTTTCAGTGATGTTTTTGAAGAGGAAATCAACATGGGCGAGTACGCTGGCAG TGACAAACTGTACCATCAGTGGCTCTCCACTGTCCGGAAAGGAAGTGGAGCAATTCTGAATACTGTAAAG ACCAAAGCAAATCCGGCCATGAAGACTGTCTACAAGTTCGCAAAAGATCATGCAAAAATGGGAATAAAAG AGGTGAAAAACCGCTTGAAGCAAAAGGACATTGCCGAGAATGGCTGCGCCCCCACCCCAGAAGAGCAGCT GCCAAAGACTGCACCGTCCCCACTGGTGGAGGCCAAGGACCCCAAGCTCCGAGAAGACCGGCGGCCAATC ACAGTCCACTTTGGACAGGTGCGCCCACCTCGTCCACATGTTGTTAAGAGACCAAAGAGCAACATCGCAG TGGAAGGCCGGAGGACGTCTGTGCCGAGCCCTGAGCAGCCGCAGCCGTATCGGACACTCAGGGAGTCAGA CAGCGCGGAAGGCGACGAGGCAGAGAGTCCAGAGCAGCAAGTGCGGAAGTCCACAGGCCCTGTCCCAGCT CCCCCTGACCGGGCTGCCAGCATCGACCTTCTGGAAGACGTCTTCAGCAACCTGGACATGGAGGCCGCAC TGCAGCCACTGGGCCAGGCCAAGAGCTTAGAGGACCTTCGTGCCCCCAAAGACCTGAGGGAGCAGCCAGG GACCTTTGACTATCAGAGGCTGGATCTGGGCGGGAGTGAGAGGAGCCGCGGGGTGACAGTGGCCTTGAAG CTTACCCACCCGTACAACAAGCTCTGGAGCCTGGGCCAGGACGACATGGCCATCCCCAGCAAGCCCCCAG CTGCCTCCCCTGAGAAGCCCTCGGCCCTGCTCGGGAACTCCCTGGCCCTGCCTCGAAGGCCCCAGAACCG GGACAGCATCCTGAACCCCAGTGACAAGGAGGAGGTGCCCACCCCTACTCTGGGCAGCATCACCATCCCC CGGCCCCAAGGCAGGAAGACCCCAGAGCTGGGCATCGTGCCTCCACCGCCCATTCCCCGCCCGGCCAAGC TCCAGGCTGCCGGCGCCGCACTTGGTGACGTCTCAGAGCGGCTGCAGACGGATCGGGACAGGCGAGCTGC CCTGAGTCCAGGGCTCCTGCCTGGTGTTGTCCCCCAAGGCCCCACTGAACTGCTCCAGCCGCTCAGCCCT GGCCCCGGGGCTGCAGGCACGAGCAGTGACGCCCTGCTCGCCCTCCTGGACCCGCTCAGCACAGCCTGGT CAGGCAGCACCCTCCCGTCACGCCCCGCCACCCCGAATGTAGCCACCCCATTCACCCCCCAATTCAGCTT CCCCCCTGCAGGGACACCCACCCCATTCCCACAGCCACCACTCAACCCCTTTGTCCCATCCATGCCAGCA GCCCCACCCACCCTGCCCCTGGTCTCCACACCAGCCGGGCCTTTCGGGGCCCCTCCAGCTTCCCTGGGGC CGGCTTTTGCGTCCGGCCTCCTGCTGTCCAGTGCTGGCTTCTGTGCCCCTCACAGGTCTCAGCCCAACCT CTCCGCCCTCTCCATGCCCAACCTCTTTGGCCAGATGCCCATGGGCACCCACACGAGCCCCCTACAGCCG CTGGGTCCCCCAGCAGTTGCCCCGTCGAGGATCCGAACGTTGCCCCTGGCCCGCTCAAGTGCCAGGGCTG CTGAGACCAAGCAGGGGCTGGCCCTGAGGCCTGGAGACCCCCCGCTTCTGCCTCCCAGGCCCCCTCAAGG CCTGGAGCCAACACTGCAGCCCTCTGCTCCTCAACAGGCCAGAGACCCCTTTGAGGATTTGTTACAGAAA ACCAAGCAAGACGTGAGCCCGAGTCCGGCCCTGGCCCCGGCCCCAGACTCGGTGGAGCAGCTCAGGAAGC AGTGGGAGACCTTCGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_020946 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020946.1, NP_065997.1 |
| RefSeq Size | 5041 bp |
| RefSeq ORF | 3030 bp |
| Locus ID | 57706 |
| UniProt ID | Q8TEH3 |
| Gene Summary | Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010] Transcript Variant: This variant (1) encodes isoform 1. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212248 | DENND1A (Myc-DDK-tagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 1 |
CNY 7,024.00 |
|
| RC212248L3 | Lenti-ORF clone of DENND1A (Myc-DDK-tagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 1 |
CNY 11,970.00 |
|
| RC212248L4 | Lenti-ORF clone of DENND1A (mGFP-tagged)-Human DENN/MADD domain containing 1A (DENND1A), transcript variant 1 |
CNY 11,970.00 |
|
| RG212248 | DENND1A (tGFP-tagged) - Human DENN/MADD domain containing 1A (DENND1A), transcript variant 1 |
CNY 8,624.00 |
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