HE4 (WFDC2) (NM_080736) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HE4, WAP5, MGC57529, dJ461P17.6
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_080736 edited ATGCCTGCTTGTCGCCTAGGCCCGCTAGCCGCCGCCCTCCTCCTCAGCCTGCTGCTGTTC GGCTTCACCCTAGTCTCAGGCACAGGAGCAGAGAAGACTGGCGTGTGCCCCGAGCTCCAG GCTGACCAGAACTGCACGCAAGAGTGCGTCTCGGACAGCGAATGCGCCGACAACCTCAAG TGCTGCAGCGCGGGCTGTGCCACCTTCTGCTCTCTGCCCAATGCACTGTTCCACTGGCAC CTAAAGACACGGAGGCTCTGGGAGATTTCTGGCCCTAGGCCACGAAGGCCCACTTGGGAC TCAAGCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_080736 unedited AATAAATTGTGCCTCCTAATCGTAAACGACTCCTATAGGGCGGCCGTGAATTATGCCTGC TTGTCGCCTAGGCCCGCTAGCCGCCGCCCTCCTCCTCAGCCTGCTGCTGTTCGGCTTCAC CCTAGTCTCAGGCACAGGAGCAGAGAAGACTGGCGTGTGCCCCGAGCTCCAGGCTGACCA GAACTGCACGCAAGAGTGCGTCTCGGACAGCGAATGCGCCGACAACCTCAAGTGCTGCAG CGCGGGCTGTGCCACCTTCTGCTCTCTGCCCATGCACTGTTCCACTGGCACCTAAAGACA CGGAGGCTCTGGGAGATTTCTGGCCCTAGGCCACGAAGGCCCACTTGGGACTCAAGCTGA CTCGACTCTAAATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATC CCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCA GCCTTGTACTAATAAAATTAACTTGCATCATTTAGACTGACTAAGTGTCCTTCCATAATA TTATCGGGTCCAGGGGGGTGGTATGGAGCCAGGCACATTAAAGAATATAACTAGTAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_080736 unedited AGGCACGGAAACTAGGGCAGGAGAGGCACCGGGGAGGGGTAAGGGAGCACCCGGGATTGC CGGAAAAGCCACGACCGCGGCCGCAATCTAGAGACGAGACAGCACGAGCCCCAAGCGGGC CCCCGTGGCCTAGGGCCAGAAATCTCCCAGAGCCTCCGTGCCCTTAGGCGCCAGTGGAAC AGTGCATCGGGCAGAGAGCAGAAGGTGGCACAGCCCGCGCCGCAGCACCCGAGGTCGTCG GCGCATACGCTGTCCGAGACGCACTCTTGCGTGCAGTTCTGGTCAGCCTGGAGCTCGGGG CACACGCCAGTCTTCTCTGCTCCTGTGCCTGAGACTAGGGTGAAGCCGAACAGCAGCAGG CTGAGGAGGAGGGCGGCGGCTAGCGGGCCTAGGCGACAAGCAGGCATGAATTCGCGGCCG CCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATA GACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACA TTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTG GAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCAAAA CCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGAAAGTCC CGTAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATA GGGGCGGACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAATA CTCCACCCATTGACGTCAATGGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGT
Restriction Sites	Please inquire
ACCN	NM_080736
Insert Size	300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_080736.1, NP_542774.1
RefSeq Size	358 bp
RefSeq ORF	309 bp
Locus ID	10406
Protein Families	Secreted Protein, Transmembrane
Gene Summary	This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) contains an alternate 3' end including a portion of the coding region and the 3' UTR, as compared to variant 1. Isoform 2 encoded by this variant thus has a distinct C-terminus from that of isoform 1, and it is smaller and contains only one WFDC domain.

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