PDE8A (NM_173454) Human Untagged Clone
CAT#: SC306815
PDE8A (untagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 2
CNY 15,110.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HsT19550 |
Vector | pCMV6 series |
Sequence Data |
>NCBI ORF sequence for NM_173454, the custom clone sequence may differ by one or more nucleotides
ATGGGCTGTGCCCCGAGCATCCACATTTCCGAGCGCCTGGTGGCCGAGGACGCGCCTAGC CCCGCGGCACCGCCGCTGTCGTCCGGCGGGCCGCGCCTCCCGCAGGGCCAGAAGACGGCC GCCTTGCCCCGGACCCGCGGCGCCGGCCTCTTGGAGTCGGAGCTTCGCGACGGCAGCGGC AAGAAGGTAGCAGTAGCTGATGTGCAGTTTGGCCCCATGAGATTTCATCAAGATCAACTT CAGGTACTTTTAGTGTTTACCAAAGAAGATAACCAATGTAATGGATTCTGCAGGGCATGT GAAAAAGCAGGGTTTAAGTGTACAGTTACCAAGGAGGCTCAGGCTGTCCTTGCCTGTTTC CTGGACAAACATCATGACATTATCATCATAGACCACAGAAATCCTCGACAGCTGGATGCA GAGGCACTGTGCAGGTCTATCAGATCATCAAAACTCTCAGAAAACACAGTTATTGTTGGT GTAGTACGCAGGGTGGATAGAGAAGAGTTGTCCGTAATGCCTTTCATTTCTGCTGGATTT ACAAGGAGGTATGTAGAAAACCCCAACATCATGGCCTGCTACAATGAACTGCTCCAGCTG GAGTTTGGAGAGGTGCGATCACAACTGAAACTCAGGGCTTGTAACTCAGTATTCACTGCA TTAGAAAACAGTGAAGATGCAATTGAAATTACAAGCGAAGACCGTTTTATACAGGAGTGG CAAGGAATTTACTATGCCAAAAAGAAAAACGGAGATAATATACAACAAAATGTGAAGATA ATACCTGTCATTGGACAGGGAGGAAAAATTAGACACTATGTGTCCATTATCAGAGTGTGC AATGGCAACAATAAGGCTGAGAAAATATCCGAATGTGTTCAGTCTGACACTCATACAGAT AATCAGACAGGCAAACATAAAGACAGGAGAAAAGGCTCACTAGACGTCAAAGCTGTTGCC TCCCGTGCAACTGAAGTTTCCAGCCAGAGACGACACTCTTCCATGGCCCGGATACATTCC ATGACAATTGAGGCGCCCATCACCAAGGTAATCAATATTATCAATGCTGCCCAGGAAAGT AGTCCCATGCCTGTGACAGAAGCCCTAGACCGTGTGCTGGAAATTCTAAGAACCACTGAG TTATATTCACCACAGTTTGGTGCTAAAGATGATGATCCCCATGCCAATGACCTTGTTGGG GGCTTAATGTCTGATGGTTTGCGAAGACTATCAGGGAATGAATATGTTCTTTCAACAAAA AACACTCAAATGGTTTCAAGCAATATAATCACTCCCATCTCCCTTGATGATGTCCCACCA CGGATAGCTCGGGCCATGGAAAATGAGGAATACTGGGACTTTGATATTTTTGAACTGGAG GCTGCCACCCACAATAGGCCTTTGATTTATCTTGGTCTCAAAATGTTTGCTCGCTTTGGA ATCTGTGAATTCTTACACTGCTCCGAGTCAACGCTAAGATCATGGTTACAAATTATCGAA GCCAATTATCATTCCTCCAATCCCTACCACAATTCTACACATTCTGCTGATGTGCTTCAT GCCACTGCCTATTTTCTCTCCAAGGAGAGGATAAAGGAAACTTTAGATCCAATTGATGAG GTCGCTGCACTCATCGCAGCCACCATTCATGATGTGGATCACCCTGGGAGAACCAACTCC TTCCTGTGTAATGCTGGAAGTGAGCTGGCCATTTTGTACAATGACACTGCTGTGCTGGAG AGCCACCATGCGGCCTTGGCCTTCCAGCTGACCACTGGAGATGATAAATGCAATATATTT AAAAACATGGAGAGGAATGATTATCGGACACTGCGCCAGGGGATTATCGACATGGTCTTA GCCACAGAAATGACAAAGCACTTTGAGCATGTCAACAAATTTGTCAACAGCATCAACAAA CCCTTGGCAACACTAGAAGAAAATGGGGAAACTGATAAAAACCAGGAAGTGATAAACACT ATGCTTAGGACTCCAGAGAACCGGACCCTAATCAAACGAATGCTGATTAAATGTGCTGAT GTGTCCAATCCCTGCCGACCCCTGCAGTACTGCATCGAGTGGGCTGCACGCATTTCGGAA GAATATTTTTCTCAGACTGATGAAGAGAAGCAGCAGGGCTTACCTGTGGTGATGCCAGTG TTTGACAGAAATACCTGCAGCATCCCCAAATCCCAAATCTCTTTCATTGATTACTTCATC ACAGACATGTTTGATGCTTGGGATGCCTTTGTAGACCTGCCTGATTTAATGCAGCATCTT GACAACAACTTTAAATACTGGAAAGGACTGGACGAAATGAAGCTGCGGAACCTCCGACCA CCTCCTGAATAG |
Restriction Sites | Please inquire |
ACCN | NM_173454 |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_173454.1, NP_775656.1 |
RefSeq Size | 3793 bp |
RefSeq ORF | 2352 bp |
Locus ID | 5151 |
UniProt ID | O60658 |
Protein Families | Druggable Genome |
Protein Pathways | Progesterone-mediated oocyte maturation, Purine metabolism |
Gene Summary | The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217197 | PDE8A (Myc-DDK-tagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 2 |
CNY 8,270.00 |
|
RC217197L3 | Lenti-ORF clone of PDE8A (Myc-DDK-tagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 2 |
CNY 10,170.00 |
|
RC217197L4 | Lenti-ORF clone of PDE8A (mGFP-tagged)-Human phosphodiesterase 8A (PDE8A), transcript variant 2 |
CNY 10,170.00 |
|
RG217197 | PDE8A (tGFP-tagged) - Human phosphodiesterase 8A (PDE8A), transcript variant 2 |
CNY 9,120.00 |