Phospholipase C epsilon 1 (PLCE1) (NM_016341) Human Untagged Clone

CAT#: SC308896

PLCE1 (untagged)-Human phospholipase C, epsilon 1 (PLCE1), transcript variant 1



  "NM_016341" in other vectors (2)

CNY 26,790.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PLCE1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms NPHS3; PLCE; PPLC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC308896 representing NM_016341.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGACTTCTGAAGAAATGACAGCTTCTGTTCTCATACCTGTGACTCAGAGAAAAGTGGTTTCTGCCCAG
TCGGCTGCAGATGAAAGTAGTGAAAAGGTCTCAGACATCAATATTTCAAAAGCACATACTGTCAGACGA
AGTGGGGAGACTTCTCATACCATCTCACAACTGAACAAACTTAAAGAAGAACCTTCTGGAAGCAACTTG
CCAAAGATTCTCTCAATAGCGAGGGAGAAAATAGTGAGTGATGAGAACAGTAATGAAAAATGTTGGGAG
AAAATCATGCCAGATTCTGCGAAAAACCTTAACATTAACTGCAACAACATATTGAGAAACCATCAGCAT
GGCCTTCCTCAGAGACAATTTTATGAAATGTACAACTCTGTTGCTGAGGAAGACTTGTGTTTAGAAACT
GGAATTCCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAATTCAACTGGAACTAGACAGACCTTCCATG
GGCATTAGTCCTTTAGGAAATCAGTCAGTGATCATAGAGACAGGCAGAGCACACCCTGACAGCAGAAGG
GCAGTATTTCATTTTCATTATGAAGTTGACAGAAGAATGTCAGACACTTTCTGTACCCTATCAGAAAAC
TTAATTTTAGACGATTGTGGAAATTGTGTACCACTACCTGGGGGTGAGGAGAAGCAAAAGAAAAACTAT
GTGGCATATACCTGTAAACTGATGGAATTGGCCAAAAATTGTGATAATAAGAATGAGCAGCTGCAGTGT
GATCATTGTGACACCTTGAATGATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAAGGTTGACATGGTA
TATTCAGGTGATAGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGCTGCCAAGACCTTTTTGAGCCAT
TTTGAGGACTTCCCTGATAATTGTGATGATGTAGAAGAAGACGCTTTTAAAAGCAAAAAGGAGCGATCC
ACTTTGTTAGTCAGGAGATTCTGTAAAAATGACAGAGAAGTTAAGAAATCTGTGTATACTGGAACAAGA
GCAATTGTGAGAACTCTGCCTTCTGGCCACATTGGGCTGACTGCATGGAGTTACATAGATCAGAAGAGA
AATGGTCCCTTACTGCCTTGTGGGAGAGTAATGGAACCCCCGTCAACAGTGGAGATAAGGCAAGATGGG
AGCCAACGTCTGTCAGAAGCCCAGTGGTATCCTATCTACAATGCAGTGAGAAGAGAAGAAACAGAAAAT
ACAGTTGGATCTCTACTCCATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGCCCATGGAAGGATAAGC
GTTGGTCCATGCTTAAAGCAATGTGTCCGAGACACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACT
TCAATATCGCAGTACATCACCGGTTCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTT
CTCAGTACTTTTGGAGGATCCACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCC
AATTCCAATGCCCTCCCTTCAAGTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTCATC
CAGTTCCCAGAGGAAGTCGCCAGCATCCTGATGGAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCCA
GTCGACTACCTTTGCTTCTTAACACGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTTGCCCTGCCTC
AAAGCATCCATCTCAGCGTCGATTCTTACCACTCAGAATGGAGAGCACAATGCCCTTGAAGATCTGGTG
ATGAGGTTTAATGAGGTGAGCTCCTGGGTGACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAG
CGAGAAGTCTTTTCATATTTGGTGCATGTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTC
ATGGAGTTCTTGGCTGGCCTCAGGTCAAGAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGAT
ATTGAGACCATGAGGAGCCTGAAGGATGCTATGGCCCAGCATGAGTCCTCTTGTGAGTACAGAAAGGTG
GTGACACGTGCCCTGCACATCCCTGGCTGTAAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGAGCTC
TGTGAAGTGCTTGACGGCGCCTCCGGTCTCATGAAGCTTTGCCCGCGGTACAATTCCCAAGAAGAAACT
TTAGAGTTTGTAGCAGATTACAGTGGACAAGATAATTTCTTACAACGAGTGGGACAAAATGGCTTAAAG
AATTCGGAGAAGGAGTCCACTGTCAACAGCATCTTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAG
ACAGACGAGGAGGACAGCCCCAGTGAAGGAAACAGCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGA
TGGCAGTTTATAATTGGAGATTTGTTGGATTCAGACAATGACATCTTTGAGCAATCCAAAGAATACGAC
TCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGACCATGGGACGGAGCTCATCCCTTGGTACGTGCTG
TCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGGGGGCCACGGTCATCCACTACGACCAGGACACA
CACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGACAATAGCACCTTGACCTGGGTAAAGCCCACA
ACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTTGGTGTACTTAATAACACAGCTGAGCCTGGAAAATTC
CCACTACTGGGTAATGCTGGATTAAGTAGCCTGACGGAAGGGGTCTTGGATCTTTTTGCAGTGAAGGCT
GTATACATGGGCCACCCTGGCATTGATATACACACTGTGTGTGTTCAGAACAAACTGGGTAGCATGTTC
CTGTCAGAGACTGGTGTGACATTGCTCTATGGGCTTCAGACCACAGACAACAGATTATTGCACTTCGTG
GCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGGAACTCACTAGAGCTGTGAGAAAGATG
AGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTACGTCAGCCTTTATCAGGAGGATGGA
CGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTGTTTGGTGGCAGACGGTGGAGTGCTCGAAAC
CCCAGCCCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAATATGCAGAGAAACAATACCCTGGGCATA
AGCACTACCAAGAAAAAGAAGAAAATCCTCATGAGGGGTGAGAGTGGAGAGGTAACTGACGATGAGATG
GCAACCCGAAAGGCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGTTCTGATCCTCAAGACATTAAT
GAACAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCCTCCCTTCCAGAAGAGCCCAC
TCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCCCATCAGGCCAGTGTCCTCC
CCTGTGCTGTCTTCTTCAAACAAGAGCCCATCCAGTGCTTGGAGCAGTAGTAGCTGGCACGGGCGGATC
AAAGGCGGCATGAAGGGATTTCAGAGCTTCATGGTTTCAGATAGCAACATGAGTTTTGTTGAATTTGTT
GAGCTGTTCAAATCATTCAGTGTCAGGAGCCGCAAGGACCTGAAGGATCTGTTTGATGTCTATGCAGTG
CCCTGCAACCGATCTGGCTCCGAGTCAGCCCCACTCTACACCAACCTGACAATTGATGAAAACACCAGC
GATCTTCAGCCTGACCTAGATCTGTTGACCAGAAATGTCTCGGATTTGGGGTTGTTCATTAAGAGTAAA
CAGCAGCTATCGGACAACCAGAGGCAGATATCTGATGCCATTGCTGCTGCAAGCATTGTGACAAATGGC
ACTGGGATTGAGAGCACATCTCTGGGCATTTTTGGGGTGGGCATACTTCAGCTCAACGATTTCCTCGTG
AATTGCCAAGGAGAACACTGCACTTATGATGAAATCCTCAGCATCATCCAGAAGTTCGAGCCTAGCATC
AGTATGTGTCATCAGGGACTAATGTCATTTGAAGGGTTTGCCAGGTTTCTGATGGATAAAGAAAATTTT
GCCTCAAAAAATGATGAGTCACAGGAGAACATTAAAGAACTGCAGCTACCCCTCTCATACTATTACATC
GAATCTTCGCACAATACCTACCTCACGGGCCATCAGCTCAAAGGAGAATCCTCGGTAGAACTCTACAGC
CAGGTCCTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACTGCTGGGACGGAGACGATGGGATGCCCATC
ATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGTTGAAGCCATTGATCGCAGT
GCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAGAACCACTGTTCATTGCCTCAGCAACGA
AAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAGCTGGTGACTAAATTCTTATTTGAGACTGAT
TTCTCAGATGATCCAATGCTTCCTTCACCTGACCAACTCAGAAAGAAAGTTCTTCTTAAAAACAAGAAG
CTAAAAGCCCATCAGACGCCAGTGGATATCTTAAAGCAAAAGGCTCATCAGTTAGCATCTATGCAAGTG
CAGGCTTATAATGGTGGGAATGCCAACCCCCGACCTGCCAATAATGAGGAAGAGGAAGATGAGGAGGAC
GAATATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGAAGACAGACCTGAAAATAAATCA
TGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGGATAAAGAAAGCAGATAACTCT
GCTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAATTTTATCTTGATCAGAATAAAAAG
GAAAGCAGACAGATTGCACCAGAGCTTTCTGACCTTGTAATCTATTGTCAAGCAGTAAAATTTCCAGGA
CTGTCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAAAGAAAGGAAAAGCAGGAAGTCCATTTTTGGC
AACAATCCGGGCAGAATGAGCCCAGGGGAGACAGCATCATTTAACAAAACATCTGGAAAAAGTTCCTGT
GAAGGCATTCGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCAACCACGTCCCTCAGTGCTATC
ATTAGAACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAACGTCTGTGTCGCAGG
TATTCTCAGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTACCCTGCTGCCACCCGCATC
GACTCTTCCAACCCGAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTGGCACTCAACTACCAG
ACTGATGATCTCCCTTTACATTTAAATGCTGCAATGTTTGAGGCAAATGGTGGTTGTGGTTATGTATTG
AAACCTCCAGTTCTGTGGGACAAGAACTGCCCCATGTATCAGAAGTTTTCTCCACTAGAAAGAGATCTG
GACAGCATGGATCCTGCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAATGTGTGCCCCAGTAATAGC
ATGGGAAGCCCGTGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTCCGCACAAAG
CCCATCCATCGAAACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCACGTTCACTTCGAAGAT
CTTGTATTTCTTCGTTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTAACTGCTCAGAGAATCATTCCA
CTGAAAGCTTTAAAACGAGGATATCGACATCTTCAGCTGCGAAACCTTCACAATGAAGTCTTGGAGATT
TCTAGTTTATTCATTAACAGCAGAAGGATGGAAGAAAATTCCTCTGGCAATACCATGTCAGCCTCTTCG
ATGTTTAATACAGAAGAAAGAAAATGTTTGCAGACTCACAGAGTCACGGTGCATGGGGTCCCAGGGCCA
GAGCCCTTTACCGTTTTCACTATTAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTGACA
AATGAACAAGACATCAAACCTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATATCTAAA
GAAAAGAATGAATGTAGGAAACAACCATTCCAGAGAGCCATTGGTCCAGAAGAGGAGATCATGCAAATT
TTAAGCAGCTGGTTTCCAGAAGAGGGATACATGGGCAGGATTGTCTTAAAAACCCAGCAGGAAAACCTA
GAAGAGAAAAACATTGTTCAAGATGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTC
CAAGTGCATGATGTTTCTCCAGAGCAACCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACTGCACAG
GATGTCATTCAGCAGACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGC
GACTATGTGCTTTTGGAAGAGGTGGTGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCT
CAGCGGGTCCTTCTGGATCAGGAGTGTGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCAGGAAAATTC
ATCCTTAAGCTAAAGGAGCAGGTGCAGGCATCTCGAGAAGATAAAAAGAAAGGCATTTCTTTCGCAAGT
GAACTCAAGAAGCTCACCAAGTCAACTAAACAGCCCCGAGGACTTACATCACCTTCTCAGCTCTTGACC
TCAGAAAGTATCCAAACCAAGGAGGAGAAACCTGTGGGTGGCTTGTCCTCCAGTGACACAATGGATTAC
CGACAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_016341
Insert Size 6909 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016341.3
RefSeq Size 7992 bp
RefSeq ORF 6909 bp
Locus ID 51196
UniProt ID Q9P212
Domains C2, RA, PI-PLC-X, PI-PLC-Y, RasGEF
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW 258.7 kDa
Gene Summary This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1; also known as PLCepsilon1a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

其它Phospholipase C epsilon 1产品

Customer Reviews 
Loading...