DOCK4 (NM_014705) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309436 representing NM_014705. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGATACCTACGGAGCACGAGAAATACGGCGTGGTTATTGCCAGTTTCCGAGGAACCGTTCCATAT GGCCTGTCATTGGAAATTGGAGATACAGTTCAGATCCTGGAGAAGTGTGATGGCTGGTACAGAGGATTT GCCTTAAAAAACCCAAATATCAAGGGTATATTTCCTTCCAGCTACGTTCACTTGAAAAATGCCTGTGTA AAGAACAAAGGACAATTTGAAATGGTTATTCCCACTGAAGACTCTGTTATCACAGAAATGACATCAACA TTAAGAGACTGGGGAACCATGTGGAAACAACTCTATGTGCGTAATGAAGGCGATCTCTTCCACCGGCTG TGGCACATCATGAATGAAATCCTGGACCTGAGGCGGCAGGTGCTGGTGGGCCACCTCACCCACGACCGG ATGAAGGACGTGAAGCGCCACATTACTGCCCGGCTTGACTGGGGCAATGAACAACTGGGACTGGACCTG GTGCCTAGGAAAGAGTACGCAATGGTGGATCCGGAAGACATCAGCATTACTGAGCTCTACCGATTGATG GAACATCGACATCGGAAGAAAGACACCCCGGTGCAGGCCAGCAGTCACCACCTCTTTGTCCAGATGAAG AGCCTCATGTGTTCCAACCTGGGAGAGGAGCTGGAGGTCATCTTCTCACTCTTTGACAGTAAAGAGAAC CGGCCAATCAGTGAGAGATTTTTCTTGAGGCTGAATAGAAACGGGCTTCCCAAAGCCCCTGATAAACCG GAACGACATTGCTCCCTCTTTGTGGATTTGGGCAGCAGTGAGCTAAGAAAGGACATTTATATCACCGTG CACATTATCCGAATCGGTCGAATGGGAGCAGGAGAAAAAAAGAATGCCTGTAGTGTCCAGTACCGACGA CCCTTTGGCTGTGCAGTTCTTAGCATCGCTGACCTGCTAACAGGAGAGACAAAGGATGACCTCATTCTG AAAGTATACATGTGTAACACAGAGAGTGAGTGGTACCAAATCCATGAGAACATCATCAAAAAGCTGAAT GCACGTTATAACTTGACTGGCTCCAATGCAGGTTTAGCAGTTTCCTTACAGCTATTGCACGGAGACATT GAACAAATCAGAAGGGAATATTCATCAGTATTTTCTCATGGAGTATCCATAACAAGGAAGCTGGGATTT TCAAATATTATTATGCCTGGTGAAATGAGGAATGATTTATATATCACTATTGAAAGGGGAGAATTTGAG AAAGGAGGGAAGAGCGTGGCCAGAAATGTGGAAGTTACGATGTTCATTGTAGACAGTAGTGGCCAAACC CTGAAGGATTTTATCTCCTTCGGCTCTGGGGAGCCACCAGCCAGTGAGTACCACTCCTTTGTGCTTTAC CATAACAACAGTCCCAGGTGGTCTGAACTGCTGAAACTTCCCATTCCTGTGGATAAATTCCGGGGTGCA CACATCCGCTTCGAGTTTCGGCATTGTTCCACAAAGGAGAAAGGAGAGAAGAAGTTGTTTGGGTTTTCT TTTGTCCCTCTGATGCAAGAAGATGGTAGGACTCTTCCAGATGGCACTCATGAGCTCATCGTGCATAAG TGTGAAGAAAACACAAATCTTCAGGATACTACCCGCTACCTCAAACTTCCCTTTTCCAAGGGCATTTTC CTTGGGAATAATAATCAAGCCATGAAGGCCACAAAGGAGTCCTTTTGTATTACATCTTTTCTCTGTTCC ACAAAACTCACACAAAATGGTGATATGCTTGATCTTTTGAAATGGAGAACCCACCCAGACAAGATCACT GGCTGTCTCTCTAAATTAAAAGAAATTGATGGCTCAGAGATAGTAAAGTTTCTGCAGGATACACTGGAT ACCTTATTTGGAATTTTAGATGAAAATTCCCAAAAATATGGGTCTAAAGTGTTTGATTCTTTGGTTCAC ATAATAAATTTGCTGCAAGATAGCAAATTTCATCATTTTAAACCTGTAATGGACACTTACATTGAGAGT CATTTTGCTGGGGCACTTGCATACAGAGATCTCATCAAAGTGCTCAAATGGTACGTGGACCGGATCACA GAAGCAGAGCGGCAAGAGCATATCCAGGAGGTGCTGAAGGCACAAGAATACATTTTTAAGTATATAGTT CAATCTCGAAGGCTGTTTTCCCTTGCCACTGGTGGGCAAAACGAAGAGGAGTTCCGCTGCTGCATTCAG GAGCTTCTCATGTCAGTCCGTTTCTTTCTTTCGCAAGAGAGCAAAGGGTCTGGAGCATTATCTCAGTCA CAGGCTGTGTTTCTGAGCTCTTTCCCTGCCGTGTACTCAGAACTGTTGAAGCTCTTTGATGTCCGGGAA GTAGCCAACTTGGTCCAGGACACCCTGGGCAGTCTGCCGACCATCCTGCATGTGGATGATTCCCTGCAG GCCATCAAACTGCAGTGCATTGGCAAAACCGTGGAAAGCCAGCTTTATACCAACCCAGATTCCCGATAC ATTCTTCTGCCTGTCGTGTTACATCACCTCCACATTCACTTGCAAGAACAGAAGGACCTGATCATGTGT GCACGTATCCTTAGCAACGTATTTTGTCTTATCAAGAAAAATAGCTCAGAAAAATCTGTGCTGGAGGAA ATAGATGTGATAGTGGCCAGCTTGCTGGATATTCTGCTGAGGACCATATTGGAGATCACCAGCCGACCT CAGCCATCCAGCTCAGCAATGCGGTTCCAGTTCCAGGATGTCACTGGGGAGTTTGTTGCTTGTCTCCTG TCCCTATTACGACAAATGACAGATAGACATTATCAACAGCTTCTTGATAGTTTTAATACAAAGGAAGAA CTAAGGGATTTCCTGCTGCAGATATTTACTGTGTTCCGAATATTGATACGCCCGGAGATGTTTCCAAAG GACTGGACTGTTATGCGCTTGGTTGCTAACAATGTTATTATTACAACAGTTCTATACCTCTCAGATGCA CTTCGTAAGAACTTCTTAAATGAAAACTTTGATTATAAGATCTGGGATTCCTACTTTTACCTCGCAGTC ATTTTTATAAACCAGTTGTGTCTGCAGTTGGAGATGTTCACACCTTCCAAGAAGAAAAAGGTGTTAGAA AAGTATGGTGACATGCGGGTAACAATGGGTTGTGAAATTTTCAGCATGTGGCAAAACCTAGGAGAGCAC AAGCTTCATTTTATCCCTGCCCTGATTGGCCCCTTCCTAGAAGTGACCTTGATACCCCAGCCAGATCTT CGGAATGTCATGATTCCAATTTTTCATGATATGATGGACTGGGAGCAGAGGCGGAGTGGCAACTTTAAA CAGGTGGAAGCCAAGCTAATTGACAAACTGGATAGCCTGATGTCAGAAGGCAAAGGTGACGAAACATAC CGCGAGCTCTTCAACAGTATTCTACTAAAGAAAATTGAGCGGGAAACATGGCGGGAAAGTGGCGTTTCA TTAATTGCTACTGTAACTCGTCTAATGGAGAGGTTGTTAGATTACAGGGACTGCATGAAAATGGGAGAG GTAGATGGCAAAAAGATTGGCTGCACAGTTAGCCTTCTGAACTTCTATAAGACTGAACTGAACAAGGAG GAGATGTATATACGCTACATTCACAAACTCTATGATCTGCATCTCAAAGCACAGAACTTTACAGAAGCT GCATATACCCTCCTCTTATATGACGAGCTACTGGAATGGTCTGATCGGCCCCTCAGGGAGTTCCTGACC TACCCCATGCAAACAGAATGGCAGCGCAAAGAGCACCTGCACCTCACCATCATCCAGAACTTTGACAGA GGCAAATGTTGGGAGAATGGCATTATCTTGTGCCGGAAGATTGCAGAGCAGTATGAGAGTTATTATGAC TACAGAAACCTGAGCAAGATGCGGATGATGGAAGCCTCTTTGTATGACAAAATTATGGACCAGCAACGT CTTGAACCAGAGTTCTTCAGAGTTGGATTTTATGGAAAAAAATTTCCATTTTTCTTAAGAAATAAGGAG TTTGTGTGTCGAGGGCATGACTACGAGAGGCTGGAAGCCTTCCAACAGAGAATGCTGAACGAGTTCCCC CATGCCATCGCCATGCAGCACGCCAACCAGCCCGATGAGACCATCTTCCAGGCAGAAGCTCAGTATTTG CAGATATATGCTGTGACTCCCATTCCAGAGAGCCAGGAGGTCCTGCAGAGAGAGGGTGTTCCGGACAAC ATCAAAAGCTTCTATAAAGTGAATCACATCTGGAAATTCCGCTATGACCGACCATTTCACAAAGGCACA AAAGATAAAGAGAATGAATTCAAGAGTCTCTGGGTGGAGAGAACGTCATTATACTTGGTGCAGAGTTTG CCTGGCATCTCTCGCTGGTTTGAAGTGGAAAAGCGTGAAGTGGTAGAAATGAGTCCTCTGGAAAATGCA ATTGAAGTGCTAGAAAATAAGAATCAGCAGCTGAAGACTCTGATTAGTCAGTGTCAGACAAGACAGATG CAGAATATTAATCCCCTGACTATGTGCCTGAATGGAGTTATAGATGCTGCAGTTAATGGTGGCGTTTCC AGGTATCAAGAGGCATTCTTTGTCAAAGAATATATCTTAAGTCACCCTGAAGATGGGGAGAAAATTGCA CGATTAAGAGAGCTGATGCTTGAGCAGGCACAGATTCTGGAATTTGGTTTGGCCGTGCATGAGAAGTTT GTACCTCAAGATATGAGACCCCTTCACAAAAAGCTGGTTGACCAATTCTTTGTGATGAAGTCGAGCTTA GGGATACAGGAGTTCTCTGCTTGTATGCAAGCCAGTCCTGTCCATTTTCCTAATGGAAGCCCTCGTGTG TGTAGAAACTCAGCACCTGCTTCTGTGAGCCCAGATGGTACCAGGGTAATTCCTAGACGCAGCCCGTTA AGTTACCCAGCTGTCAACCGATATTCTTCCTCCTCACTGTCCTCACAAGCTTCTGCTGAAGTAAGCAAT ATTACAGGGCAATCAGAAAGCTCTGATGAAGTCTTTAACATGCAGCCAAGTCCATCTACCTCAAGCTTG AGTTCTACTCACTCGGCTTCACCTAATGTGACAAGTTCTGCTCCATCGAGTGCCAGAGCTTCTCCTTTG TTGTCTGACAAACACAAACATTCCCGAGAAAACTCTTGCCTGTCACCAAGAGAGAGACCATGCAGTGCC ATCTATCCAACACCTGTGGAGCCTTCGCAGAGGATGCTGTTTAATCATATTGGAGACGGGGCCTTGCCA CGCAGTGACCCAAATCTCTCTGCACCTGAAAAAGCTGTGAACCCCACCCCTAGCAGCTGGAGCCTGGAC AGTGGGAAGGAAGCCAAGAACATGTCGGATAGTGGGAAACTTATCTCTCCCCCTGTCCCTCCAAGACCC ACACAGACTGCTTCACCAGCAAGACACACGACATCAGTATCCCCCTCGCCTGCCGGGCGATCTCCATTG AAGGGCTCTGTGCAGTCTTTCACCCCCTCTCCAGTGGAGTACCACTCGCCAGGACTCATCTCCAACTCC CCTGTCTTGTCGGGCAGCTACAGCAGTGGGATTTCTTCTCTCAGCCGGTGCAGCACGTCGGAAACCTCA GGCTTTGAAAATCAGGTGAATGAACAGTCGGCCCCCCTGCCGGTGCCAGTGCCGGTGCCCGTGCCGAGC TACGGCGGGGAGGAGCCAGTGCGCAAGGAGAGCAAGACTCCGCCCCCGTACAGCGTCTACGAGCGGACT CTGCGGCGCCCCGTCCCGCTACCTCACAGCCTCTCCATCCCCGTCACGTCGGAGCCGCCCGCGCTGCCC CCCAAGCCTCTGGCAGCGCGATCCAGCCACCTGGAGAATGGGGCCCGGAGGACTGACCCCGGCCCGCGG CCCAGGCCCCTGCCCCGCAAGGTCTCTCAGTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_014705
Insert Size	5901 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014705.3
RefSeq Size	8343 bp
RefSeq ORF	5901 bp
Locus ID	9732
UniProt ID	Q8N1I0
MW	225.2 kDa
Gene Summary	This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.