ATRX (NM_000489) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JMS; MRX52; RAD54; RAD54L; XH2; XNP; ZNF-HX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309873 representing NM_000489. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCGCTGAGCCCATGAGTGAAAGCAAGTTGAATACATTGGTGCAGAAGCTTCATGACTTCCTTGCA CACTCATCAGAAGAATCTGAAGAAACAAGTTCTCCTCCACGACTTGCAATGAATCAAAACACAGATAAA ATCAGTGGTTCTGGAAGTAACTCTGATATGATGGAAAACAGCAAGGAAGAGGGAACTAGCTCTTCAGAA AAATCCAAGTCTTCAGGATCGTCACGATCAAAGAGGAAACCTTCAATTGTAACAAAGTATGTAGAATCA GATGATGAAAAACCTTTGGATGATGAAACTGTAAATGAAGATGCGTCTAATGAAAATTCAGAAAATGAT ATTACTATGCAGAGCTTGCCAAAAGGTACAGTGATTGTACAGCCAGAGCCAGTGCTGAATGAAGACAAA GATGATTTTAAAGGGCCTGAATTTAGAAGCAGAAGTAAAATGAAAACTGAAAATCTCAAAAAACGCGGA GAAGATGGGCTTCATGGGATTGTGAGCTGCACTGCTTGTGGACAACAGGTCAATCATTTTCAAAAAGAT TCCATTTATAGACACCCTTCATTGCAAGTTCTTATTTGTAAGAATTGCTTTAAGTATTACATGAGTGAT GATATTAGCCGTGACTCAGATGGAATGGATGAACAATGTAGGTGGTGTGCGGAAGGTGGAAACTTGATT TGTTGTGACTTTTGCCATAATGCTTTCTGCAAGAAATGCATTCTACGCAACCTTGGTCGAAAGGAGTTG TCCACAATAATGGATGAAAACAACCAATGGTATTGCTACATTTGTCACCCAGAGCCTTTGTTGGACTTG GTCACTGCATGTAACAGCGTATTTGAGAATTTAGAACAGTTGTTGCAGCAAAATAAGAAGAAGATAAAA GTTGACAGTGAAAAGAGTAATAAAGTATATGAACATACATCCAGATTTTCTCCAAAGAAGACTAGTTCA AATTGTAATGGAGAAGAAAAGAAATTAGATGATTCCTGTTCTGGCTCTGTAACCTACTCTTATTCCGCA CTAATTGTGCCCAAAGAGATGATTAAGAAGGCAAAAAAACTGATTGAGACCACAGCCAACATGAACTCC AGTTATGTTAAATTTTTAAAGCAGGCAACAGATAATTCAGAAATCAGTTCTGCTACAAAATTACGTCAG CTTAAGGCTTTTAAGTCTGTGTTGGCTGATATTAAGAAGGCTCATCTTGCATTGGAAGAAGACTTAAAT TCCGAGTTTCGAGCGATGGATGCTGTAAACAAAGAGAAAAATACCAAAGAGCATAAAGTCATAGATGCT AAGTTTGAAACAAAAGCACGAAAAGGAGAAAAACCTTGTGCTTTGGAAAAGAAGGATATTTCAAAGTCA GAAGCTAAACTTTCAAGAAAACAGGTAGATAGTGAGCACATGCATCAGAATGTTCCAACAGAGGAACAA AGAACAAATAAAAGTACCGGTGGTGAACATAAGAAATCTGATAGAAAAGAAGAACCTCAATATGAACCT GCCAACACTTCTGAAGATTTAGACATGGATATTGTGTCTGTTCCTTCCTCAGTTCCAGAAGACATTTTT GAGAATCTTGAGACTGCTATGGAAGTTCAGAGTTCAGTTGATCATCAAGGGGATGGCAGCAGTGGAACT GAACAAGAAGTGGAGAGTTCATCTGTAAAATTAAATATTTCTTCAAAAGACAACAGAGGAGGTATTAAA TCAAAAACTACAGCTAAAGTAACAAAAGAATTATATGTTAAACTCACTCCTGTTTCCCTTTCTAATTCC CCAATTAAAGGTGCTGATTGTCAGGAAGTTCCACAAGATAAAGATGGCTATAAAAGTTGTGGTCTGAAC CCCAAGTTAGAGAAATGTGGACTTGGACAGGAAAACAGTGATAATGAGCATTTGGTTGAAAATGAAGTT TCATTACTTTTAGAGGAATCTGATCTTCGAAGATCCCCACGTGTAAAGACTACACCCTTGAGGCGACCG ACAGAAACTAACCCTGTAACATCTAATTCAGATGAAGAATGTAATGAAACAGTTAAGGAGAAACAAAAA CTATCAGTTCCAGTGAGAAAAAAGGATAAGCGTAATTCTTCTGACAGTGCTATAGATAATCCTAAGCCT AATAAATTGCCAAAATCTAAGCAATCAGAGACTGTGGATCAAAATTCAGATTCTGATGAAATGCTAGCA ATCCTCAAAGAGGTGAGCAGGATGAGTCACAGTTCTTCTTCAGATACTGATATTAATGAAATTCATACA AACCATAAGACTTTGTATGATTTAAAGACTCAGGCGGGGAAAGATGATAAAGGAAAAAGGAAACGAAAA AGTTCTACATCTGGCTCAGATTTTGATACTAAAAAGGGCAAATCAGCTAAGAGCTCTATAATTTCTAAA AAGAAACGACAAACCCAGTCTGAGTCTTCTAATTATGACTCAGAATTAGAAAAAGAGATAAAGAGCATG AGTAAAATTGGTGCTGCCAGAACCACCAAAAAAAGAATTCCAAATACAAAAGATTTTGACTCTTCTGAA GATGAGAAACACAGCAAAAAAGGAATGGATAATCAAGGGCACAAAAATTTGAAGACCTCACAAGAAGGA TCATCTGATGATGCTGAAAGAAAACAAGAGAGAGAGACTTTCTCTTCAGCAGAAGGCACAGTTGATAAA GACACGACCATCATGGAATTAAGAGATCGACTTCCTAAGAAGCAGCAAGCAAGTGCTTCCACTGATGGT GTCGATAAGCTTTCTGGGAAAGAGGAGAGTTTTACTTCTTTGGAAGTTAGAAAAGTTGCTGAAACTAAA GAAAAGAGCAAGCATCTCAAAACCAAAACATGTAAAAAAGTACAGGATGGCTTATCTGATATTGCAGAG AAATTCCTAAAGAAAGACCAGAGCGATGAAACTTCTGAAGATGATAAAAAGCAGAGCAAAAAGGGAACT GAAGAAAAAAAGAAACCTTCAGACTTTAAGAAAAAAGTAATTAAAATGGAACAACAGTATGAATCTTCA TCTGATGGCACTGAAAAGTTACCTGAGCGAGAAGAAATTTGTCATTTTCCTAAGGGCATAAAACAAATT AAGAATGGAACAACTGATGGAGAAAAGAAAAGTAAAAAAATAAGAGATAAAACTTCTAAAAAGAAGGAT GAATTATCTGATTATGCTGAGAAGTCAACAGGGAAAGGAGATAGTTGTGACTCTTCAGAGGATAAAAAG AGTAAGAATGGAGCATATGGTAGAGAGAAGAAAAGGTGCAAGTTGCTTGGAAAGAGTTCAAGGAAGAGA CAAGATTGTTCATCATCTGATACTGAGAAATATTCCATGAAAGAAGATGGTTGTAACTCTTCTGATAAG AGACTGAAAAGAATAGAATTGAGGGAAAGAAGAAATTTAAGTTCAAAGAGAAATACTAAGGAAATACAA AGTGGCTCATCATCATCTGATGCTGAGGAAAGTTCTGAAGATAATAAAAAGAAGAAGCAAAGAACTTCA TCTAAAAAGAAGGCAGTCATTGTCAAGGAGAAAAAGAGAAACTCCCTAAGAACAAGCACTAAAAGGAAG CAAGCTGACATTACATCCTCATCTTCTTCTGATATAGAAGATGATGATCAGAATTCTATAGGTGAGGGA AGCAGCGATGAACAGAAAATTAAGCCTGTGACTGAAAATTTAGTGCTGTCTTCACATACTGGATTTTGC CAATCTTCAGGAGATGAAGCCTTATCTAAATCAGTGCCTGTCACAGTGGATGATGATGATGACGACAAT GATCCTGAGAATAGAATTGCCAAGAAGATGCTTTTAGAAGAAATTAAAGCCAATCTTTCCTCTGATGAG GATGGATCTTCAGATGATGAGCCAGAAGAAGGGAAAAAAAGAACTGGAAAACAAAATGAAGAAAACCCA GGAGATGAGGAAGCAAAAAATCAAGTCAATTCTGAATCAGATTCAGATTCTGAAGAATCTAAGAAGCCA AGATACAGACATAGGCTTTTGCGGCACAAATTGACTGTGAGTGACGGAGAATCTGGAGAAGAAAAAAAG ACAAAGCCTAAAGAGCATAAAGAAGTCAAAGGCAGAAACAGAAGAAAGGTGAGCAGTGAAGATTCAGAA GATTCTGATTTTCAGGAATCAGGAGTTAGTGAAGAAGTTAGTGAATCCGAAGATGAACAGCGGCCCAGA ACAAGGTCTGCAAAGAAAGCAGAGTTGGAAGAAAATCAGCGGAGCTATAAACAGAAAAAGAAAAGGCGA CGTATTAAGGTTCAAGAAGATTCATCCAGTGAAAACAAGAGTAATTCTGAGGAAGAAGAGGAGGAAAAA GAAGAGGAGGAGGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAAGATGAAAATGATGATTCCAAGTCT CCTGGAAAAGGCAGAAAGAAAATTCGGAAGATTCTTAAAGATGATAAACTGAGAACAGAAACACAAAAT GCTCTTAAGGAAGAGGAAGAGAGACGAAAACGTATTGCTGAGAGGGAGCGTGAGCGAGAAAAATTGAGA GAGGTGATAGAAATTGAAGATGCTTCACCCACCAAGTGTCCAATAACAACCAAGTTGGTTTTAGATGAA GATGAAGAAACCAAAGAACCTTTAGTGCAGGTTCATAGAAATATGGTTATCAAATTGAAACCCCATCAA GTAGATGGTGTTCAGTTTATGTGGGATTGCTGCTGTGAGTCTGTGAAAAAAACAAAGAAATCTCCAGGT TCAGGATGCATTCTTGCCCACTGTATGGGCCTTGGTAAGACTTTACAGGTGGTAAGTTTTCTTCATACA GTTCTTTTGTGTGACAAACTGGATTTCAGCACGGCGTTAGTGGTTTGTCCTCTTAATACTGCTTTGAAT TGGATGAATGAATTTGAGAAGTGGCAAGAGGGATTAAAAGATGATGAGAAGCTTGAGGTTTCTGAATTA GCAACTGTGAAACGTCCTCAGGAGAGAAGCTACATGCTGCAGAGGTGGCAAGAAGATGGTGGTGTTATG ATCATAGGCTATGAGATGTATAGAAATCTTGCTCAAGGAAGGAATGTGAAGAGTCGGAAACTTAAAGAA ATATTTAACAAAGCTTTGGTTGATCCAGGCCCTGATTTTGTTGTTTGTGATGAAGGCCATATTCTAAAA AATGAAGCATCTGCTGTTTCTAAAGCTATGAATTCTATACGATCAAGGAGGAGGATTATTTTAACAGGA ACACCACTTCAAAATAACCTAATTGAGTATCATTGTATGGTTAATTTTATCAAGGAAAATTTACTTGGA TCCATTAAGGAGTTCAGGAATAGATTTATAAATCCAATTCAAAATGGTCAGTGTGCAGATTCTACCATG GTAGATGTCAGAGTGATGAAAAAACGTGCTCACATTCTCTATGAGATGTTAGCTGGATGTGTTCAGAGG AAAGATTATACAGCATTAACAAAATTCTTGCCTCCAAAACACGAATATGTGTTAGCTGTGAGAATGACT TCTATTCAGTGCAAGCTCTATCAGTACTACTTAGATCACTTAACAGGTGTGGGCAATAATAGTGAAGGT GGAAGAGGAAAGGCAGGTGCAAAGCTTTTCCAAGATTTTCAGATGTTAAGTAGAATATGGACTCATCCT TGGTGTTTGCAGCTAGACTACATTAGCAAAGAAAATAAGGGTTATTTTGATGAAGACAGTATGGATGAA TTTATAGCCTCAGATTCTGATGAAACCTCCATGAGTTTAAGCTCCGATGATTATACAAAAAAGAAGAAA AAAGGGAAAAAGGGGAAAAAAGATAGTAGCTCAAGTGGAAGTGGCAGTGACAATGATGTTGAAGTGATT AAGGTCTGGAATTCAAGATCTCGGGGAGGTGGTGAAGGAAATGTGGATGAAACAGGAAACAATCCTTCT GTTTCTTTAAAACTGGAAGAAAGTAAAGCTACTTCTTCTTCTAATCCAAGCAGCCCAGCTCCAGACTGG TACAAAGATTTTGTTACAGATGCTGATGCTGAGGTTTTAGAGCATTCTGGGAAAATGGTACTTCTCTTT GAAATTCTTCGAATGGCAGAGGAAATTGGGGATAAAGTCCTTGTTTTCAGCCAGTCCCTCATATCTCTG GACTTGATTGAAGATTTTCTTGAATTAGCTAGTAGGGAGAAGACAGAAGATAAAGATAAACCCCTTATT TATAAAGGTGAGGGGAAGTGGCTTCGAAACATTGACTATTACCGTTTAGATGGTTCCACTACTGCACAG TCAAGGAAGAAGTGGGCTGAAGAATTTAATGATGAAACTAATGTGAGAGGACGATTATTTATCATTTCT ACTAAAGCAGGATCTCTAGGAATTAATCTGGTAGCTGCTAATCGAGTAATTATATTCGACGCTTCTTGG AATCCATCTTATGACATCCAGAGTATATTCAGAGTTTATCGCTTTGGACAAACTAAGCCTGTTTATGTA TATAGGTTCTTAGCTCAGGGAACCATGGAAGATAAGATTTATGATCGGCAAGTAACTAAGCAGTCACTG TCTTTTCGAGTTGTTGATCAGCAGCAGGTGGAGCGTCATTTTACTATGAATGAGCTTACTGAACTTTAT ACTTTTGAGCCAGACTTATTAGATGACCCTAATTCAGAAAAGAAGAAGAAGAGGGATACTCCCATGCTG CCAAAGGATACCATACTTGCAGAGCTCCTTCAGATACATAAAGAACACATTGTAGGATACCATGAACAT GATTCTCTTTTGGACCACAAAGAAGAAGAAGAGTTGACTGAAGAAGAAAGAAAAGCAGCTTGGGCTGAG TATGAAGCAGAGAAGAAGGGACTGACCATGCGTTTCAACATACCAACTGGGACCAATTTACCCCCTGTC AGTTTCAACTCTCAAACTCCTTATATTCCTTTCAATTTGGGAGCCCTGTCAGCAATGAGTAATCAACAG CTGGAGGACCTCATTAATCAAGGAAGAGAAAAAGTTGTAGAAGCAACAAACAGTGTGACAGCAGTGAGG ATTCAACCTCTTGAGGATATAATTTCAGCTGTATGGAAGGAGAACATGAATCTCTCAGAGGCCCAAGTA CAGGCGTTAGCATTAAGTAGACAAGCCAGCCAGGAGCTTGATGTTAAACGAAGAGAAGCAATCTACAAT GATGTATTGACAAAACAACAGATGTTAATCAGCTGTGTTCAGCGAATACTTATGAACAGAAGGCTCCAG CAGCAGTACAATCAGCAGCAACAGCAACAAATGACTTATCAACAAGCAACACTGGGTCACCTCATGATG CCAAAGCCCCCAAATTTGATCATGAATCCTTCTAACTACCAGCAGATTGATATGAGAGGAATGTATCAG CCAGTGGCTGGTGGTATGCAGCCACCACCATTACAGCGTGCACCACCCCCAATGAGAAGCAAAAATCCA GGACCTTCCCAAGGGAAATCAATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_000489
Insert Size	7479 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000489.4
RefSeq Size	11238 bp
RefSeq ORF	7479 bp
Locus ID	546
UniProt ID	P46100
Domains	SNF2_N, DEAD, helicase_C
Protein Families	Druggable Genome, Transcription Factors
MW	282.6 kDa
Gene Summary	The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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