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TLE 1 (TLE1) (NM_005077) Human Untagged Clone
CAT＃: SC310123
TLE1 (untagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1)
CNY 5,648.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ESG; ESG1; GRG1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005077 edited
ACCGCTGTGACTACTCTGGACTCGCCCGCAGAGTTTGCCCGCCGGGGAGGGTGGCCGATT GGCGGAGCGCACTCCTGCTGTTTTCCCCACCATCTCGTGGATGTCGTCCCTCGGGTGGTG AGAGAACTTTGCAGTGGGCTGGAGCGCCCTTTGCGGAGAAGCACACGAAGGTGAAGGAAG GAAAGAAAGAAGACCGAGGAAGAAGGAGCTCGGAAGAAGGGTCCGGAGCGGCCGGTCCGG CCGTGCAGGGCGAGTGCGCCCGAGGCGCGGCGCCCTGATGCTCCCCGGGCTCGAGGAGCA TTGGGCACTTGCGGGACTGCTCTCGGTGGAGTACCNCCGTCGGCCCGGAAGGGGTTTGTG CAACCGCGGAGACNACCGAGTGCTGGTCGACGGGGGGGCGTGCCGAGCCGCCTCCCGGCG CGCCCTCCGCACTTTCCCCGCCTCGTCATCCGTCGCTCCCCGGCCAGGAGCCTCCGCTGC GTCTTCAACCTCGCTCCCCTTTGCGTCCCGGGAGCCTGCGAGCACCCGGCCGAAGGCGCA GCCGAATCTTGCGGGAGTCGCCCCGAAAGCGTCGGGTTTGTGTGGGGTTAGCGGGGGCCG CCGCGCCACCTGCACCTCGCCCGCCGCCGCCTCGGGGAAAGCCCGAAGAGGAGGCGGACC AGGAGAAGAGCAAAGAAAAGCAGTCCGTCTGGATTTGTTTGCCCAGGACTGGCGCCGCGC ACGCGGATCGCCGAGGGGAGTGCGGTCGGAGTCACCGCGCCCCCGCCTCCCCGCCCGGGC AGCTGAGGCCGGGGGTTGGAGCGCTGCCCCCGCGCACAGTCCCCGAGCGCCCGACGTCTC CGCGCAGGTTCTTGAAGCAGCTGGGCCTGGGGCGCCCACTAATGTGGCCCTGAGGGCCGG AGCCCGCACCGACGGGAGCGGGAGCCGGAGCAGCTGCGGGCGCCGAGTGGCCGGTGCGCC CGGCGGAGCGCGCGTGCGTGGCCAGCGCGCTCCCCGCTTCTGCTTGGCTTTCCGGCTTAA TTTTCCTCGGCGGGATTAAAGTTGGAAATTGACCGGAGAATTGAGTTGCCGGGGAACAGA GCCCCGGCCGCCGCCAGAGCGATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCT GCAGGCCAGCCCTTCAAGTTCACTATCCCGGAGTCCCTGGACCGGATTAAAGAGGAATTC CAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATGTGAGAAACTGGCAAGTGAA AAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGATTAAACATT GAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATC CCATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAG GTGACCATGGCAGAATTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTT TCTCATGGCCACGGACCCCCAGTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCT GGAATCCCGCCCCTCGGGGGCAGTGCCGGCCTTCTTGCGCTGTCTAGTGCTCTGAGTGGG CAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGATGCAGAGCACCACAGAGAC AGAGAGCCGGGCACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGCACAGATAAA CGCAGAAATGGACCTGAATTTTCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGAC TCCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCT AATGAGGACCCTTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATC GACAAAAATCGCCTGCTAAAGAAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCG GCAAGTTCCACTTCTTTGAAATCCAAAGAAATGAGCTTGCATGAAAAAGCCAGCACGCCT GTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCCAACGCCGGGCACCAGCGCC ACTCCAGGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATAGACCCCCTCGTTAACCAA GCGGCAGCTGGCTTGAGGACACCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGG ATGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGT TTACACAACATGTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGCCTAC GGGCGCTCCCCCATGGTGGGGTTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCT CCAAACCTGGCAGGAATCCCTGGGGGGAAACCTGCATACTCCTTCCACGTTACTGCAGAC GGTCAGATGCAGCCTGTCCCTTTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGG CATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATCAGC AACCCCACGAGACACGTGTACACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGC CACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATC CGTTCCTGTAAATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAGTACT TTGTCCATTTGGGACCTGGCGGCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCG GCCCCCGCCTGCTACGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGC AGCGACGGCAACATCGCTGTGTGGGATCTGCACAACCAGACACTAGTGAGGCAATTCCAG GGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACG GGTGGTTTGGACAACACAGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAG CACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCA GTGGGCATGGAGAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAG CTGCACCTGCATGAGAGCTGCGTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTT GTGAGTACTGGAAAAGATAACCTCCTCAATGCTTGGCGGACCCCCTATGGAGCCAGCATA TTCCAGTCCAAAGAGTCCTCGTCAGTGCTTAGCTGTGACATCTCTGTGGATGATAAGTAC ATAGTCACTGGCTCGGGGGACAAGAAGGCTACAGTCTATGAAGTCATCTACTGAAAACAT TATGTGGTTTAACGTTTATAGTTGAATTGGGCCAAAATGTTTCGAATTTATAGAAATAGA AAAGTTGTAACTTTAAAAGAGAAAAAAAATTACAAACACCTGTTTCCAAACCTTGACAGA AAACTACTTTGAGTCTACAAAGAGGAGGCGACAAGTCCATCAGCAGAAAGTCACCTGTCT ACATAGACCAAATGGAGCACCAAGGCCAAGCGGACAGAGGGGCCATGGGTTGTAGGATTG AGGAACGGAATCTGCCGACTCACATGACAGCCCATTCTTTCTTTCTGGGTGATCTGGGGA TCACGCCTTGCCCAAGTGTGAGATTACCTTTCTGTTCCTTGCAGTTCACCTCACTTTCCG TCCTTTGTAGAGCAGTGGTGTCTCCAATGAACTTGTTTCCTGGTTTTGCATCTTGTGAAA TGTTTTTTTGTATTTTTGTTGAAGGTTAAACATTTGTATAAATTGTAAATATATTTGGTT TATTACAGTAAAGGCTTTAGTACCAATAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_005077 unedited
NTGGGNCTTAGCTGCTTGTAACATTGCGGCCTTGGCGCCCACTATGTGCCCCTGAGGCCG GAGCCCGCACCGACGGGAGCGGGAGCCGTGACAGCTGCGGGCGCCTAGTGGCCGGTGCTC CCGGCGGAGCGCGCGTGCGTGGCCAGCGCGCTCCCCGCTTCTGCTTGGCTTTCCGGCTTA ATTTTCCTCGGCGGGATTAAAGTTGGAAATTGACCGGATAATTGTGTTGCCGGGGAACAG AGCCCCGGCCGCCGCCAGAGCGATGTTCCCGCAGAGCCGGCTCCCGACGCCGCACCATGC TGCAGGCCAGCCCTTCAAGTTCACTATCCTGGATTCCCTGGACCGGATTAAAAGAGAATT CCAGTTCCTGCGGGCGCAGTATCACATCCTTTAAATTGGAATGTGAGAAACTGGGCTGTC GACAGACTGAACTGCTCAGCCCTATGTGATGGTTTATGAAATGTCATATTGGATTAACCA TTGGAATGCACAACTAGACTTTTATCGTCTAGCAGTTTAATCCCATTTTTTTCCCATGTA TTCACTTATTTTGTTTTATGAACTTTATAGCCAGGTGGCCCCAGACTATTTTTCGTCCCA AGCAGTGTTCCAAGGCTTGATTTGAATCCTTGCTTCGTCCATAGAAAAATTCATATCTAT GATTTGATCTCTTTGGTAGTGGCGCCCATTTTTCCTTTTTACACCTATTTCTATGAGAGT CTTCTACTCCTATAAGATAAACACTTACATTATGGAGTGATGTATCCAGGNAATATATCT AATCGTCTACTATCATTCTCGTTCACCAGCATACTCCCCCTCGCCTGACCCATGTTATAT GTCAGGAGCACCCTTTTGTTTGTCCTCTATATATGTGCATGGTTAGTATTAACTACAAAA TAACAATCGCATACATACCCAACCAATAAATACT >OriGene 3' read for NM_005077 unedited
TTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTATTGGTACTAAAGCCTTTACTGTAATAAA CCAAATATATTTACAATTTATACAAATGTTTAACCTTCAACAAAAATACAAAAAAACATT TCACAAGATGCAAAACCAGGAAACAAGTTCATTGGAGACACCACTGCTCTACAAAGGACG GAAAGTGAGGTGAACTGCAAGGAACAGAAAGGTAATCTCACACTTGGGCAAGGCGTGATC CCCAGATCACCCAGAAAGAAAGAATGGGCTGTCATGTGAGTCGGCAGATTCCGTTCCTCA ATCCTACAACCCATGGCCCCTCTGTCCGCTTGGCCTTGGTGCTCCATTTGGTCTATGTAG ACAGGTGACTTTCTGCTGATGGACTTGTCGCCTCCTCTTTGTAGACTCAAAGTAGTTTTC TGTCAAGGTTTGGAAACAGGCGTTTGTAATTTTTTTTCTCTTTTAAAGTTACAACTTTTC TATTTCTATAAATTCGAAACATTTTGGCCCAATTCAACTATAAACGTTAAACCACATAAT GTTTTCAGTAGATGACTTCATAGACTGTAGCCTTCTTGTCCCCCGAGCCAGTGACTATGT ACTTATCATCCACAGAGATGTCACAGCTAACCACTGACGAGGACTTTTTGAACCTGAATA TGCTGGCTCCATAAGGGGTCCCCCCACCATTGAAGACGTTATTCTTTCCAGTCCTCCAAA CCATTTACCCCAGAAGGCAATTTCAGGGACAGCCCGCCCTTTCATGCCGGTGCACTGGGA CTTCCAAGCTTGTTAACTGCAAAACCTCCAATGGTGTTTTCTGCCAATGCCACCACTCCC GGTGGGCATACCCCGTGAAAAACTTGGAGGGATTCTGCTTCCGCCCGGCCCCCTCCCGAG GCCCAGACCGGACTGTGTTCAAACAACGTGCAGACTGGGGCCACATTTAAATCCATACAC GTGTTCCTTTGCTGCCCCGCTAATTTTTATTTN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_005077 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005077.3, NP_005068.2 |
| RefSeq Size | 3283 bp |
| RefSeq ORF | 2313 bp |
| Locus ID | 7088 |
| UniProt ID | Q04724 |
| Domains | WD40, TLE_N |
| Protein Families | Transcription Factors |
| Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204037 | TLE1 (Myc-DDK-tagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1) |
CNY 8,456.00 |
|
| RC204037L1 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), Myc-DDK-tagged |
CNY 10,856.00 |
|
| RC204037L2 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), mGFP tagged |
CNY 10,856.00 |
|
| RC204037L3 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), Myc-DDK-tagged |
CNY 10,856.00 |
|
| RC204037L4 | Lenti ORF clone of Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), mGFP tagged |
CNY 10,856.00 |
|
| RG204037 | TLE1 (tGFP-tagged) - Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1) |
CNY 10,056.00 |
