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Epsin 2 (EPN2) (NM_148921) Human Untagged Clone
CAT＃: SC310244
EPN2 (untagged)-Human epsin 2 (EPN2), transcript variant 1
CNY 7,220.00
| Cited in 3 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EHB21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310244 representing NM_148921.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACGACTTCGTCTATCAGACGGCAGATGAAAAACATCGTGAACAATTACTCAGAGGCAGAAATCAAA GTCCGGGAAGCCACCTCCAATGACCCGTGGGGCCCGTCCAGTTCTCTGATGACCGAGATTGCCGACCTG ACCTACAACGTGGTGGCCTTCTCGGAGATCATGAGCATGGTGTGGAAGCGGCTGAATGACCATGGCAAG AACTGGCGGCATGTGTACAAGGCGCTGACCCTGCTGGACTACCTCATCAAGACAGGCTCCGAACGTGTG GCCCAGCAGTGCCGGGAGAACATCTTCGCCATCCAGACCCTGAAGGACTTCCAGTACATTGACCGAGAT GGCAAGGACCAGGGCATCAATGTGCGTGAGAAGTCAAAGCAACTGGTGGCTCTCCTCAAGGACGAGGAA CGGTTGAAGGCTGAGAGGGCCCAGGCTCTCAAAACCAAAGAGCGCATGGCCCAGGTTGCCACTGGCATG GGCAGCAACCAGATCACCTTTGGGCGAGGCTCCAGCCAGCCCAACCTCTCCACCAGCCACTCGGAGCAG GAGTATGGCAAGGCCGGGGGCTCCCCGGCCTCCTACCATGGCTCCACCTCCCCGCGAGTGTCCTCCGAG CTGGAGCAAGCCCGGCCCCAGACTAGTGGAGAAGAGGAGCTTCAGCTGCAGCTGGCACTTGCCATGAGC AGAGAAGTGGCTGAGCAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGATTACAGATGGCCCTGGAA GAAAGCCGAAGGGACACAGTTAAAATTCCAAAAAAGAAAGAGCATGGCTCTCTCCCACAGCAGACTACG CTGTTGGATTTAATGGATGCTCTCCCCAGCTCGGGCCCCGCGGCCCAGAAAGCAGAGCCCTGGGGCCCG TCAGCCTCCACTAACCAGACCAACCCCTGGGGCGGGCCAGCGGCTCCTGCGAGTACTTCAGACCCCTGG CCATCGTTTGGTACCAAGCCAGCTGCCTCCATTGACCCATGGGGGGTGCCCACTGGAGCCACCGTACAA TCTGTCCCCAAGAACTCGGACCCCTGGGCAGCTTCACAGCAGCCTGCCTCCAGTGCTGGGAAAAGAGCT TCTGACGCGTGGGGCGCAGTCTCCACCACCAAGCCCGTGTCTGTCTCTGGGTCCTTTGAGCTCTTCAGT AATCTGAATGGTACAATTAAAGATGACTTTTCTGAATTTGACAACCTTCGGACTTCAAAAAAAACAGCC GAATCTGTGACCTCTCTGCCATCCCAAAACAATGGAACTACCAGCCCTGACCCCTTTGAGTCTCAACCC CTGACTGTCGCCTCAAGCAAGCCCAGCAGTGCCCGGAAAACACCTGAGTCCTTCCTGGGCCCCAACGCG GCCCTGGTGAACCTGGACTCACTGGTGACCAGGCCTGCCCCACCAGCCCAGTCCCTCAACCCTTTCCTG GCACCAGGTGCTCCCGCCACCTCGGCCCCTGTTAACCCTTTCCAGGTGAACCAGCCCCAGCCGCTGACA CTGAACCAGCTTCGGGGGAGCCCAGTCCTGGGGACCAGCACATCCTTTGGGCCTGGCCCAGGAGTGGAG TCCATGGCTGTGGCCTCGATGACCTCCGCGGCCCCACAGCCAGCTCTGGGGGCCACTGGTTCCTCTCTG ACACCACTGGGCCCTGCAATGATGAACATGGTGGGCAGTGTGGGTATACCCCCATCAGCAGCCCAGGCC ACTGGCACAACCAACCCTTTCCTTCTCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_148921 |
| Insert Size | 1755 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_148921.3 |
| RefSeq Size | 4664 bp |
| RefSeq ORF | 1755 bp |
| Locus ID | 22905 |
| UniProt ID | O95208 |
| Domains | ENTH, UIM |
| Protein Pathways | Endocytosis |
| MW | 62.3 kDa |
| Gene Summary | This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 2. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Effects of alternative splicing on function of Bestrophin-1 calcium-activated chloride channels
,null,
The Biochemical journal
,PubMed ID 24341532
[EPN2]
|
|
DCPIB, the Proposed Selective Blocker of Volume-Regulated Anion Channels, Inhibits Several Glutamate Transport Pathways in Glial Cells
,Nicole H. Bowens, Preeti Dohare, Yu-Hung Kuo, and Alexander A. Mongin,
Mol. Pharmacol., Jan 2013; 83: 22 - 32.
[EPN2]
|
|
,null,
Molecular Pharmacology
,PubMed ID 23012257
[EPN2]
|
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Resources
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| RC210899L4 | Lenti ORF clone of Human epsin 2 (EPN2), transcript variant 1, mGFP tagged |
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| RG210899 | EPN2 (tGFP-tagged) - Human epsin 2 (EPN2), transcript variant 1 |
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