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Calcineurin A (PPP3CA) (NM_000944) Human Untagged Clone
CAT＃: SC310299
PPP3CA (untagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1
CNY 5,832.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACCIID; CALN; CALNA; CALNA1; CCN1; CNA1; IECEE; IECEE1; PPP2B |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000944 edited
AGAAAATAAATAAATAACCACCACACACGCGCAGCCCGGAGCGAGTCGGCGGGGCTGGCG GCAGCGGCGGAGGAGGAGTGAAGGCGGCGGCGGCGGAGGAGGGACGCGCGGAAAAGGCAG CAACTTTAAAGCCAGCTCAGAGCCTAGACCTCCAGCCGAGTGGTTTGCAGCGCGGCGGCG GCGGCGGCGGCGGCGGCGGCGGCGTTGAGTGTCTGGCCCGCCGGTCCGGTCGGGGTGTGC AGTCGGACGGACGAGCAGCGCGTCGCTGTCCTCCGGCAGCTGGAGATGTCCGAGCCCAAG GCAATTGATCCCAAGTTGTCGACGACCGACAGGGTGGTGAAAGCTGTTCCATTTCCTCCA AGTCACCGGCTTACAGCAAAAGAAGTGTTTGATAATGATGGAAAACCTCGTGTGGATATC TTAAAGGCGCATCTTATGAAGGAGGGAAGGCTGGAAGAGAGTGTTGCATTGAGAATAATA ACAGAGGGTGCATCAATTCTTCGACAGGAAAAAAATTTGCTGGATATTGATGCGCCAGTC ACTGTTTGTGGGGACATTCATGGACAATTCTTTGATTTGATGAAGCTCTTTGAAGTCGGG GGATCTCCTGCCAACACTCGCTACCTCTTCTTAGGGGACTATGTTGACAGAGGGTACTTC AGTATTGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTCTACCCCAAAACACTGTTT TTACTTCGTGGAAATCATGAATGTAGACATCTAACAGAGTATTTCACATTTAAACAAGAA TGTAAAATAAAGTATTCAGAACGCGTATATGATGCCTGTATGGATGCCTTTGACTGCCTT CCCCTGGCTGCCCTGATGAACCAACAGTTCCTGTGTGTGCATGGTGGTTTGTCTCCAGAG ATTAACACTTTAGATGATATCAGAAAATTAGACCGATTCAAAGAACCACCTGCATATGGA CCTATGTGTGATATCCTGTGGTCAGACCCCCTGGAAGATTTTGGAAATGAGAAGACTCAG GAACATTTCACTCACAACACAGTCAGGGGGTGTTCATACTTCTACAGTTACCCGGCTGTA TGTGAATTCTTACAGCACAATAACTTGTTATCTATACTCCGAGCCCACGAAGCCCAAGAT GCAGGGTACCGCATGTACAGGAAAAGCCAAACAACAGGCTTCCCTTCTCTAATTACAATT TTTTCAGCACCAAATTACTTAGATGTATACAATAACAAAGCTGCAGTATTGAAGTATGAG AACAATGTTATGAATATCAGGCAATTCAACTGTTCTCCTCATCCATACTGGCTTCCAAAT TTCATGGATGTTTTTACTTGGTCCCTTCCATTTGTTGGGGAAAAAGTGACTGAGATGCTG GTAAATGTCCTCAACATCTGCTCAGATGATGAACTAGGGTCAGAAGAAGATGGATTTGAT GGTGCAACAGCTGCAGCCCGGAAAGAGGTGATAAGGAACAAGATCCGAGCAATAGGCAAA ATGGCCAGAGTGTTCTCAGTGCTCAGAGAAGAGAGTGAGAGTGTGCTGACGCTGAAAGGC TTGACCCCAACTGGCATGCTCCCCAGCGGAGTACTTTCTGGAGGGAAGCAAACCCTGCAA AGCGCTACTGTTGAGGCTATTGAGGCTGATGAAGCTATCAAAGGATTTTCACCACAACAT AAGATCACTAGCTTCGAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCT CGCAGAGATGCCATGCCCTCTGACGCCAACCTTAACTCCATCAACAAGGCTCTCACCTCA GAGACTAACGGCACGGACAGCAATGGCAGTAATAGCAGCAATATTCAGTGACCACTTCCT GTTCACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGCTGCGGGGCATGATGGGGATTGC TGCATATCAGCAGTTGGATGTTCTTGCCTCTGACAGTAGCTTATTTGCTCTGGGGGCCAG GAATTGGATTCAGTTTACACTATCATTAAAAAAGAGGGAGAGAGATAATAAACTATATTT TGGTGGGGATGGTGATTAAACACCTCTTTTGGGTATGCCTTTTAAAAATGCTTATAGAGA AAAAAAATTTTAAAAAGAAAGCTAATGCTAGTATATACTGCAATGTTAGGGGAATGAACA TGTTTTCCTACTGCATTGGGGACTTCTAGATAGGTTAATGAAAGGCCTTTTATTCTGTTA CTGGACATGAAAACTTTGTCTAATTTCTTACTCTATTGTACGTTTACAGTCGCAGCACTA AAAATGGATGACATCAAACATTTTTAACAAAATGATGTACAAACTAAGGACTATTTATTG ATAATGTTTTGCTACTCTTGTCAGACAATGGCTATAAACTGAATTAGGCAGTCTTAAAAA AAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000944 |
| Insert Size | 2400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this clone has been fully sequenced and found to be a perfect match to the protein associated with this reference, NM_000944.3. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000944.2, NP_000935.1 |
| RefSeq Size | 4425 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 5530 |
| UniProt ID | Q08209 |
| Domains | Metallophos, PP2Ac |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway |
| Gene Summary | Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:23468591, PubMed:27974827, PubMed:22343722). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed:30611118).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219136 | PPP3CA (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1 |
CNY 5,816.00 |
|
| RC219136L1 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1, Myc-DDK-tagged |
CNY 8,216.00 |
|
| RC219136L2 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1, mGFP tagged |
CNY 8,216.00 |
|
| RC219136L3 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1, Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC219136L4 | Lenti ORF clone of Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1, mGFP tagged |
CNY 8,216.00 |
|
| RG219136 | PPP3CA (tGFP-tagged) - Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 1 |
CNY 7,416.00 |
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