DCAMKL2 (DCLK2) (NM_001040260) Human Untagged Clone

CAT#: SC310962

DCLK2 (untagged)-Human doublecortin-like kinase 2 (DCLK2), transcript variant 1



  "NM_001040260" in other vectors (7)

CNY 14,820.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal DCLK2 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CL2; CLICK-II; CLICK2; CLIK2; DCAMKL2; DCDC3; DCDC3B; DCK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310962 representing NM_001040260.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCCAGCACCAGGAGTATCGAGCTGGAGCACTTTGAGGAACGGGACAAAAGGCCGCGGCCGGGGTCG
CGGAGAGGGGCCCCCAGCTCCTCCGGGGGCAGCAGCAGCTCGGGCCCCAAGGGGAACGGGCTCATCCCC
AGTCCGGCGCACAGTGCCCACTGCAGCTTCTACCGCACGCGGACCCTGCAGGCCCTCAGCTCGGAGAAG
AAGGCCAAGAAGGCGCGCTTCTACCGGAACGGGGACCGCTACTTCAAGGGCCTGGTGTTTGCCATCTCC
AGCGACCGCTTCCGGTCCTTCGATGCGCTCCTCATAGAGCTCACCCGCTCCCTGTCGGACAACGTGAAC
CTGCCCCAGGGTGTCCGCACTATCTACACCATCGACGGCAGCCGGAAGGTCACCAGCCTGGACGAGCTG
CTGGAAGGTGAGAGTTACGTGTGTGCATCCAATGAACCATTTCGTAAAGTCGATTACACCAAAAATATT
AATCCAAACTGGTCTGTGAACATCAAGGGTGGGACATCCCGAGCGCTGGCTGCTGCCTCCTCTGTGAAA
AGTGAAGTAAAAGAAAGTAAAGATTTCATCAAACCCAAGTTAGTGACTGTGATTCGAAGTGGAGTGAAG
CCTAGAAAAGCCGTGCGGATCCTTCTGAATAAAAAGACTGCTCATTCCTTTGAACAAGTCTTAACAGAT
ATCACCGAAGCCATTAAACTAGACTCAGGAGTCGTCAAGAGGCTCTGCACCCTGGATGGAAAGCAGGTT
ACTTGTCTGCAAGACTTTTTTGGTGATGACGATGTTTTTATTGCATGTGGACCAGAAAAATTTCGTTAT
GCCCAAGATGACTTTGTCCTGGATCATAGTGAATGTCGTGTCCTGAAGTCATCTTATTCTCGATCCTCA
GCTGTTAAGTATTCTGGATCCAAAAGCCCTGGGCCCTCTCGACGCAGCAAATCACCAGCTTCAGTTAAT
GGAACTCCCAGCAGCCAACTTTCTACTCCTAAATCTACGAAATCCTCCAGTTCCTCTCCAACTAGTCCA
GGAAGTTTCAGAGGATTAAAGCAGATTTCTGCTCATGGCAGATCTTCTTCCAATGTAAACGGTGGACCT
GAGCTTGACCGTTGCATAAGTCCTGAAGGTGTGAATGGAAACAGATGCTCTGAATCATCAACTCTTCTT
GAGAAATACAAAATTGGAAAGGTCATTGGTGATGGCAATTTTGCAGTAGTCAAAGAGTGTATAGACAGG
TCCACTGGAAAGGAGTTTGCCCTAAAGATTATAGACAAAGCCAAATGTTGTGGAAAGGAACACCTGATT
GAGAATGAAGTGTCAATACTGCGCCGAGTGAAACATCCCAATATCATTATGCTGGTCGAGGAGATGGAA
ACAGCAACTGAGCTCTTTCTGGTGATGGAATTGGTCAAAGGTGGAGATCTCTTTGATGCAATTACTTCG
TCGACCAAGTACACTGAGAGAGATGGCAGTGCCATGGTGTACAACTTAGCCAATGCCCTCAGGTATCTC
CATGGCCTCAGCATCGTGCACAGAGACATCAAACCAGAGAATCTCTTGGTGTGTGAATATCCTGATGGA
ACCAAGTCTTTGAAACTGGGAGACTTTGGGCTTGCGACTGTGGTAGAAGGCCCTTTATACACAGTCTGT
GGCACACCCACTTATGTGGCTCCAGAAATCATTGCTGAAACTGGCTATGGCCTGAAGGTGGACATTTGG
GCAGCTGGTGTGATCACATACATACTTCTCTGTGGATTCCCACCATTCCGAAGTGAGAACAATCTCCAG
GAAGATCTCTTCGACCAGATCTTGGCTGGGAAGCTGGAGTTTCCGGCCCCCTACTGGGATAACATCACG
GACTCTGCCAAGGAATTAATCAGTCAAATGCTTCAGGTAAATGTTGAAGCTCGGTGTACCGCGGGACAA
ATCCTGAGTCACCCCTGGGTGTCAGATGATGCCTCCCAGGAGAATAACATGCAAGCTGAGGTGACAGGT
AAACTAAAACAGCACTTTAATAATGCGCTCCCCAAACAGAACAGCACTACCACCGGGGTCTCCGTCATC
ATGAACACGGCTCTAGATAAGGAGGGGCAGATTTTCTGCAGCAAGCACTGTCAAGACAGCGGCAGGCCT
GGGATGGAGCCCATCTCTCCAGTTCCTCCCTCAGTGGAGGAGATCCCTGTGCCTGGGGAAGCAGTCCCG
GCCCCCACCCCTCCGGAATCTCCCACCCCCCACCCTCCTCCCGCTGCCCCGGGTGGTGAGCGGGCAGGA
ACCTGGCGCCGCCACCGAGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001040260
Insert Size 2301 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001040260.3
RefSeq Size 4274 bp
RefSeq ORF 2301 bp
Locus ID 166614
UniProt ID Q8N568
Protein Families Druggable Genome, Protein Kinase
MW 83.6 kDa
Gene Summary This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]
Transcript Variant: This variant (1) is known as exon-5-lacking transcript (PMIDs:15611072 and 18075264). It lacks an in-frame coding exon in the 5' region, as compared to variant 2. The resulting isoform (a) lacks an internal segment, as compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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